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Association of the 94 KDa Glucose-regulated Protein with Immunoglobulin Heavv Chain Binding Protein (BiP) (94 KDa Glucose-regulated Protein의 BiP과의 결합)

  • 강호성;김한도
    • The Korean Journal of Zoology
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    • v.35 no.4
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    • pp.456-465
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    • 1992
  • The 94 KDa glucose-resulsted Protein (SH 94), one of stress Proteins, is a Ca2+-binding protein in the endoplasmic reticulum (ER). In this study, the possible effect of Ca2+ on the native conformation of grp 94 was examined. When the purified grp 94 was analyzed by Sel filtration in the presence of either EGTA or CaCl2, it was eluted with apparent molecular weight (MW) of 100 KDa in both cases. When similarly analyzed with microtome or cell Ivsate, however, srp 94 was eluted with apparent IW of 200 KDa in the presence of E6TA, while with apparent MW of 100 KDa in the presence of CaCl2, indicating possible association of grp 94 with one or more other proteins in the absence of CaCl2. Consequently, immunoprecipitation with anti-grp 94 was carried out to determine which proteins specifically interact with grp 94. It is sho%un that srp 94 may interact, in a Ca2+_dependent manner. with other proteins including BiP (grp 78) which is also a stress protein in the ER.

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Drag Reduction Phenomena of Surfactant Turbulent Pipe Flows (계면활성제에 의한 난류 관내 유동의 마찰감소 현상)

  • Yoon, Hyung-Kee;Shin, Kwang-Ho;Chang, Ki-Chang;Ra, Ho-Sang;Yoo, Seong-Yeon
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.18 no.12
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    • pp.1025-1032
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    • 2006
  • This is to characterize the fluid mechanics of surfactant water solutions, which exhibit drag reduction in the turbulent flow as compared to pure water. The emphasis is placed on those fluid characteristic aspects of drag reducing solutions which are relevant for application in closed circulation loops for the purpose of pumping power savings, like hydronic cooling and heating systems in buildings. The experiments are carried out with the solutions of the surfactant Beraid DR-IW 616 in concentration of $100{\sim}3,000ppm$. The following key parameters are focused in this study: surfactant concentration, solution temperature and pipe diameter.

Properties of Penicillin Amidohydrolase Immobilized on Nylon Fiber

  • B. L. Seng;Iw-Han Cho;J. S. Rhee;Dewey D. Y. Ryu
    • Bulletin of the Korean Chemical Society
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    • v.1 no.1
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    • pp.10-17
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    • 1980
  • Penicillin amidohydrolase was partially purified from the fermented broth of Bacillus megaterium, and was immobilized on nylon fiber. The surface area of nylon fiber was increased by roughening it with fine sand and activated by acid treatment. The free amino groups on the nylon fiber exposed by such treatment were then utilized to immobilize the penicillin amidase. Enzymatic properties of penicillin amidohydrolase immobilized on the nylon fiber by covalent bonding and cross linking with glutaraldehyde were studied and compared with those of soluble enzyme. The optimal pH and temperature profile of immobilized enzyme showed only slightly broader peaks, and the values of kinetic constants, $K_m$, $K_{ia}$, and $K_{ip}$, of the immobilized enzyme are only slightly greater than those of the soluble enzyme. These results suggest that the mass transfer effect on the reaction rate for the penicillin amidase immobilized on nylon fiber is not so significant as the enzyme immobilized on some other support material like bentonite. The experimental results of batch reaction agreed well with the results of computer simulation for both the immobilized and soluble enzyme systems, confirming the validity of the rate equation derived which was based on the combined double inhibition by two reaction products.

Estimation of High Resolution Soil Moisture Based on Sentinel-1 SAR Sensor (Sentinel-1 SAR 센서 기반 고해상도 토양수분 산정)

  • KIm, Sangwoo;Lee, Taehwa;Shin, Yongchul
    • Proceedings of the Korea Water Resources Association Conference
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    • 2019.05a
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    • pp.141-141
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    • 2019
  • 토양수분은 수문 분석에 있어 매우 중요한 인자 중 하나이며 최근 기후변화로 인한 가뭄, 홍수 및 산불발생과 같은 물 관련 재해 발생에 직 간접적으로 영향을 미치기 때문에 지표 토양수분산정은 매우 중요하다. Sentinel-1 SAR(Synthetic Aperture Radar)는 능동형 위성으로 10m의 공간해상도로 제공되기 때문에 기존의 토양수분 전용위성인 SMOS(Soil Moisure and Ocean Salinity), SMAP(Soil Moisture Active Passive) 및 GCOM-W1(Global Change Observation Mission Water) 등 다르게 고해상도 토양수분 산정이 가능하다. 그러나 Sentinel-1 SAR 센서에서는 고해상도 지표 관측 이미지 자료만 제공하며, 토양수분 자료를 직접적으로 제공하지 않는다. 따라서 본 연구에서는 2018년도 Sentinel-1 A/B IW(Interferometric Wide swath) 모드의 VH(Vertical Transmit - Horizontal Receive) 편파 영상과 Sentinel-1 SAR 위성자료 전처리 도구인 SNAP(Sentinel Application Platform)을 이용하여 후방산란계수를 산정하였으며, 산정된 후 방산란계수와 농촌진흥청에서 제공하는 65개 지점의 실측 TDR(Time Domain Reflectrometry) 토양수분의 관계를 이용하여 회귀모형을 도출 및 토양수분 공간분포를 산정하였다. 비록 불확실성은 어느정도 발생 하였으나, 전체적으로 TDR 관측값과 $10m{\times}10m$ 해상도의 Sentinel-1 SAR 기반 토양수분이 일치하는 경향을 보였다. 본 연구 결과는 수문, 농업, 산림, 재해 등 다양한 분야에 활용될 수 있을 것으로 판단된다.

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Analysis of Water Level Change using D-InSAR Technique (D-InSAR 기법을 활용한 하천 수위 변화 분석)

  • Young Jun Bang;MinJi Seo;Hyock Jin Lim;Chi Young Kim
    • Proceedings of the Korea Water Resources Association Conference
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    • 2023.05a
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    • pp.409-409
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    • 2023
  • 하천 수위는 합리적인 수자원의 이용 및 관리를 위해 반드시 필요한 수문 자료이다. 우리나라에서는 수위 측정을 위해 유역 내에 관측소를 설치하여 장비 또는 인력을 통해 수위를 측정하고 있다. 하지만, 많은 관측소를 운영하고 관리하기에는 예산과 인력이 소모되는 한계가 있다. 위성 영상을 통한 시계열 분석은 전지구적 모니터링과 관측 분야에 중요한 역할을 수행할 것으로 기대되고 있으며, 특히 위성 영상자료를 활용한 수자원 분야 연구가 활발히 진행되고 있다. 위성 영상을 활용하여 수면적을 감지하고 수위와 유량을 판별하는 많은 연구가 진행되었지만, 하천 하상의 경사와 단면 형태에 따라 수면적이 변하여 정량적인 수위 추정에는 한계가 존재한다. 본 연구에서는 Sentinel-1의 SAR 영상과 InSAR 기법을 통해 낙동강 유역의 홍수 전후의 하천 수위 변화를 분석하였다. Sentinel-1 IW 모드의 Single Look Complex(SLC) 영상 12장과 ESA 영상 처리 툴인 SNAP을 활용하여 VV(Vertical-Vertical) 데이터의 간섭을 통해 센티미터(cm) 단위지표 변화에 따른 수위 변위를 분석하였다. 위성 영상을 통해 추출한 수위 변위와 계측 수위 및 단면 자료의 정합성을 비교한 결과, 제방과 수체 경계면 식생과 하상 세굴로 인한 오차로 정량적이 수위의 정합성에는 한계가 존재하였지만, 수위의 정량적인 변동성을 확인할 수 있었으며, 수위 변화의 반응속도를 판별할 수 있었다.

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Two cases of secondary amenorrhoea treated by Hyunburikyungtang gamibang (현부리경탕가미방(玄附理經湯加味方)을 병행한 무월경 환자 치험2례(例))

  • Koo, Jin Suk;Seo, Bu Il
    • The Korea Journal of Herbology
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    • v.31 no.2
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    • pp.7-12
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    • 2016
  • Objectives : Secondary amenorrhoea is the absence of menses for three months in a woman with previously normal menstruation or nine months for women with a history of oligomenorrhoea. It can be caused by stress, extreme weight loss, and excessive exercise. The purpose of this study was to report the clinical effects of herbal medicine on secondary amenorrhoea.Methods : We employed oriental medical treatments; herbal - medication (Hyunburikyungtang gamibang), acupuncture and moxibustion. We treated the patients one or two times a month with oriental therapy method. They took medicine three times a day after a meal. During taking medicine, we let the patients avoid fatty food, flour based food and alcohol. We evaluated the status of the patient, on the basis of the state of menstration and F2 level of Yangdorak. Because we diagnosed the condition of patients with the pattern of liver depression and qi stagnation, so F2 level of Yangdorak was an important point of the diagnosis. Yangdorak machine was Tormeter Iw - zen at Towatech Co.,Ltd.Results : After taking treatment - several times acupuncture and moxibustion during some period and taking herbal-medicine, they had menstrain naturally without taking any hormone drug. Also the amount of menstration has gradually increased. The F2 level of Yangdorak returned to normal range. The feeling of cold on hands, feet and lower abdomen was much improved.Conclusions : Herbal medicine (Hyunburikyungtang gamibang) with oriental medical treatments, acupuncture and moxibustion was effective in the treatment of secondary amenorrhoea.

Investigation of Seasonal Characteristics of Contaminants and Hydrochemical Factors in an Aquifer for Application of In Situ Reactive Zone Technology (원위치 반응존 공법 적용을 위한 대수층내 오염물질 및 환경영향인자의 계절 특성 평가)

  • Ahn, Jun-Young;Kim, Cheolyong;Kim, Tae Yoo;Jun, Seong-Chun;Hwang, Inseong
    • Journal of Soil and Groundwater Environment
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    • v.21 no.6
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    • pp.192-203
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    • 2016
  • A field investigation was conducted on an aquifer contaminated with trichloroethylene (TCE) for application of in situ reactive zone treatment using nanosized zero-valent iron (NZVI). The aquifer was an unconfined aquifer with a mean hydraulic conductivity of $5.14{\times}10^{-4}cm/sec$, which would be favorable for NZVI injection. Seasonal monitoring of TCE concentration revealed a presence of non-aqueous phase liquid form of TCE near IW (injection well). The hydrochemical data characterized the site groundwater to be a $Ca-HCO_3$ type. The average value of Langelier Saturation Index of the groundwater was -1.33, which implied that the site was favorable for corrosion of NZVI. Dissolved oxygen (DO) concentration varied between 2.5~11.5 mg/L, which indicated that DO would greatly compete with TCE as an electron acceptor. The hydrogeological and hydrochemical characterization reveals that the time around November would be appropriate for NZVI injection when water level and temperature are relatively high and DO concentration is low.

Partial Oxidation of n-Octane over Rh-Containing Alumina-Supported Catalysts (알루미나에 담지된 Rh 함유 촉매의 n-옥탄 부분산화반응)

  • Lee, Shin-Hwa;Suh, Young-Woong;Suh, Dong-Jin;Park, Tae-Jin;Lee, Kwan-Young
    • Transactions of the Korean hydrogen and new energy society
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    • v.19 no.1
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    • pp.10-17
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    • 2008
  • This study has been focused on the partial oxidation(POX) of n-octane over Rh-containing catalysts supported on alumina. The catalysts for this reaction were prepared by incipient wetness(IW) and co-gel(CG) methods, followed by the calcination at $900{\circ}C$ or $1,200{\circ}C$. When applied to the POX of n-octane carried out at $600{\circ}C$ with C/O=3 and GHSV=3,450/h, the catalyst prepared by the CG method and calcined at $1,200{\circ}C$ showed the best activity, yielding 42% syngas($H_2$+CO) with the $H_2$/CO ratio of $2{\sim}2.4$. To enhance the activity and stability of catalysts, bimetallic catalysts were synthesized by the CG method. As a result, the performance of Rh-Ni/$Al_2O_3$ catalyst was superior to that of Rh/$Al_2O_3$ catalyst in terms of the catalyst stability, due to the retarding effect on the Rh-to-$Rh_2O_3$ transition by the addition of Ni. This result was confirmed by XRD, TEM, and TPR characterizations.

The 40~50Day Intraseasonal Oscillation of the Geostationary Meteorological Satellite High Cloud Amount (GMS 상층운량의 40~50일 계절만 진동)

  • 하경자;서애숙
    • Journal of Environmental Science International
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    • v.5 no.5
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    • pp.619-633
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    • 1996
  • Intraseasonal variability of the tropical convection over the Indian/western Pacific is studied using the Geostationary Meteorological Satellite high cloud amount. This study is directed to find the tropical-extratropical interaction in the frequency range of intraseasonal and interannual variabilities of the summer monsoon occured over the domain of 90E-171W and 495-50N. Especially, in order to investigate the intraseasonal interaction of last Asia summer monsoon associated with the tropical convections in the high cloud amounts, the spatial and time structure of the intraseasonal oscillation for the movement-and the evolution of the large-scale connections are studied. To describe the spatial and the time evolution, the extended empirical orthogonal function analysis is applied. The first mode may be considered to a normal structure, indicating that the strong convection band over 90E-120E is extended to sastward but this mode was detected as the intraseasonal variability during summer monsoon. It is found that the dominant intraseasonal mode of the tropical convection consists of the spatial changes over a broad period range centered around 40~50days.

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Density Fluctuation of Tetranychus urticae and Three Predatory Mite Species(Phytoseiidae) by the Differently Infested Levels (점박이응애와 천적인 3종 이리응애의 접종수준별 밀도 변동)

  • 이영인;권기면;이순원;류하경;류언하
    • Korean journal of applied entomology
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    • v.36 no.3
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    • pp.237-242
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    • 1997
  • Comparative studies on suppression possibility with three phytoseiid mite species (An.thly,seiu,sw omersleyi Schicha. A. ,fidIrrc~i.Gs arman and T\ulcornerphlorlrotiiu.s oc~c~idetitaliNs esbit) to the two-spotted spider mite (Te~trrrt~yc.Iir~l~l\ulcorner.i\c .(re Kwh) on kidney bean leaves in field and greenhouse were carried out. In the field experiments with the initial prey -predator ratio of 4 : 1. I0 : I and 20: I . A. ,firllrrcis suppressed successfully the prey populations at all three ratios 17 days after the initial infestation. A. wornc,r-;leyi \uppressed the prey population only at the ratio of 4 : 1, while T. oc~c~ideritcr1iw.s as unable to suppress the prey population at all tested ratios. In the greenhouse experiments with the initial prey-predator ratio of 10: 1, A. jil1ltrci.s could suppress the prey population continuously during the infestation period. A. ~~otnc~r,slceoyuil d suppress the prey population for 13 days after the initial infestation, while T. occie1mttrli.s could suppress the prey population for 8 - 23 days after the initial infestation.

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