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Studies on the Sex Hormone Levels in Korean Native Goat during Puberty, Estrous Cycle and Pregnancy(I) - Studies on Reproductive Traits, and Changes of Sex Hormone Levels by Growth- (재래산양(在來山羊)의 성성숙(性成熟), 발정주기(發情週期) 및 임신기간(姙娠期間)에 따른 성(性) Hormone 수준(水準)의 변화(變化)에 관(關)한 연구(硏究)(I) -번식형질(繁殖形質)과 체성장(體成長)에 따른 혈청(血淸) 성(性) Hormone 수준(水準)의 변화(變化)-)

  • Lee, Kyu Seung;Park, Chang Sik;Kim, Young Mook
    • Korean Journal of Agricultural Science
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    • v.12 no.1
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    • pp.55-61
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    • 1985
  • This study was carried out to investigate reproductive traits and sex hormone levels by growth in Korean native goats. Serum levels of FSH, LH, prolactin, estradiol-$17{\beta}$ and progesterone were determined every 15 days from the 70 days of age to 190 days by radioimmunoassay. The age and weight at sexual maturity were 183.6 days and 14.3 kg; the length of estrous cycle and estrus period were 20.3 days and 36.7 hours, respectively. The gestation period, litter size and body weight at birth were 148.4 days, 1.4 head and 1.8 kg, respectively. The levels of serum LH were highest with 3.93 mIU/ml at 70 days of age, thereafter decreased gradually to 1.21 mIU/ml at 190 days of age. The concentrations of FSH in serum were below 1.25 mIU/ml throughout the experimental period. The levels of prolactin were lowest with 3.09ng/ml at 85 days of age and highest with 4.65 ng/ml at 175 days of age. The serum levels of estradiol-$17{\beta}$ were increased with age, thus highest with 7.95 pg/ml at 190 days of age. The serum progesterone concentrations were maintained low levels (below 1.0 ng/ml) throughout the experimental period.

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Effects of Dietary Vitamin E and Selenium Supplementation on Meat Color Stability of Hanwoo(Korean Native Cattle) Bull Beef during Retail Display (Vitamin E와 Selenium의 급여가 비거세우육의 진열저장중 육색 안정성에 미치는 영향)

  • 김용선;양성운;김주용;박연수;황환섭
    • Food Science of Animal Resources
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    • v.22 no.2
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    • pp.108-114
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    • 2002
  • The effects of dietary vitamin E and selenium(Se) supplementation on meat color stability in M. Longissimus of Hanwoo(Korean native cattle) bull beef during retail display(5$^{\circ}C$, 1,200 lux) were investigated. Experimental groups were divided into control(Vit E 27 IU/head/day, Se 0.09 mg/head/day), Vit E (2,500 IU/head/day), Se(20 mg/head/day), Vit E+Se(Vit E 2,500 IU/head/day, Se 20 mg/head/day) groups. CIE a*(redness), chroma(C*) values, oxymyoglobin(%) and R630-R580 were significantly (p<0.05) decreased among the 4 treatment groups during retail display, in particular, those values decreased more rapidly in the control group. The metmyoglobin (%) of 0 day(before storage) was not significantly (p<0.05) different among the 4 treatment groups. However, the rate of metmyoglobin accumulation during storage increased more rapidly in the control group. Therefore, discoloration in the control group was more accelerated compared to the other groups. TBARS(thiobarbituric acid reactive substances) which represent lipid rancidity was significantly(p<0.05) lower in Se and Vit E+Se groups than in the control and Vit E groups. Reducing ability of 0 day(before storage) was significantly lower in the control group than in the other groups, and it decreased more rapidly in the control group after 3 days of storage. Consequently, Se-supplemented groups(Se and Vit E+Se groups) were more resistant to lipid oxidation than were the control and Vit E groups. The stability of meat color and myoglobin forms(%) were significantly (p<0.05) higher in Vit E, Se and Vit E+Se groups than in the control group, but there were no difference among Vit E, Se and Vit E+Se groups.

Reproductive Bioligy of the Sevenband Grouper, Epinephelus septemfasciatus I. The Effect of HCG on Ovulation Induction (능성어, Epinephelus septemfasciatus의 번식생물학적 연구 I. HCG 처리에 의한 배란유도)

  • Kim, Byong-Ho;Kim, Kyong-Min;Lee, Young-Don;Song, Choon Bok;Rho, Sum
    • Journal of Aquaculture
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    • v.10 no.1
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    • pp.55-61
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    • 1997
  • The experiment was conducted to understand the effect of HCG upon ovulation induction and egg maturation in the sevenband grouper, Epinephelus septemfasciatus, as a part of the research for its resources management and seed production. Three different size groups of sevenband groupers showing 300~400g, 1,500~2,000g and 2,500~4,000g, were collected from June to August, 1996. They were treated two or three times with HCG (500~1,000 IU/kg). Gonadal development of the sevenband groupers were examined using the paraffin section method and then stained with haematoxylin and eosin. When smaller groups of sevenband grouper having 300~400g, 1,500~2,00g and 2,500~3,200g in weight were treated two or three time with HCG 9500~1,000 IU/g), their gonads contained oogonia, oocytes with the size of 20~30${\mu}{\textrm}{m}$, and stroma tissues. However, in case of two HCG treatments with a dosage of 1,000 IU/kg on sevenband groupers of 3,400~4,000g, both healthy eggs of $800\mu$m in diameter and regressing eggs were ovulated. A sevenband grouper of 4,000g obvulated 4,252 eggs in total. Of these, healthy and regressing eggs turned out to be 20.8% and 79.2%, respectively. In addition, previtellogenic oocytes of around $50\mu$m, oocytes of 20~$30\mu$m, and ovulation trace were also observed in the gonad.

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Comparison of Pregnancy Rates by Intrauterine Insemination after Ovulation Trigger with Endogenous LH Surge, GnRH Agonist or hCG in Stimulated Cycles (인공수정시술주기에서 내인성 LH, GnRH Agonist 및 hCG를 이용한 배란유발후 임신율의 비교연구)

  • Lee, Jong-In;Hur, Young-Mun;Jeon, Eun-Suk;Yoon, Jeong-Im;Jung, Goo-Sung;Hong, Ki-Eon;You, Seung-Hwan;Lee, Hyeon-Sook;Hong, Jeong-Eui;Lee, Ji-Sam
    • Clinical and Experimental Reproductive Medicine
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    • v.26 no.3
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    • pp.389-398
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    • 1999
  • Objective: This study was designed to evaluate the effects of endogenous LH surge, GnRH agonist (GnRH-a) or human chorionic gonadotropin (hCG) as ovulation trigger on pregnancy rate by intrauterine insemination (IUI). Method: Patients received daily 100 mg of clomiphene citrate (CC) for 5 days starting on the third day of the menstrual cycle followed by human menopausal gonadotropin (hMG) for ovulation induction. Follicles larger than >16 mm in diameter were present in the ovary, frequent LH tests in urine were introduced to detect an endogenous LH surge. Final follicular maturation and ovulation were induced by GnRH-a 0.1 mg (s.c.) or hCG $5,000{\sim}10,000$ IU (i.m.) administration except natural ovulation. Pregnancy was classified as clinical if a gestational sac or fetal cardiac activity was seen on ultrasound. Results: There were no differences in age, duration of infertility and follicle size, but more ampules of hMG were used in GnRH-a group compared to hCG 10,000 IU treated group (p<0.05). Lower level of estradiol ($E_2$) on the day of hCG or GnRH-a injection was observed in hCG 10,000 IU group than other treatment groups (p<0.01). The overall clinical pregnancy rate was 19.8% per cycle (32/162) and 22.2% per patient (32/144). Pregnancy rate was higher in natural-endogenous LH surge group (37.5%, 9/24) than GnRH-a (18.8%) or hCG treated group (20.9% & 13.9%), but this difference was not statistically significant. No patient developed ovarian hyperstimulation. Abortion rate was 22.2% (2/9) in hCG 5,000 IU group. Delivery or ongoing pregnancy rate was 37.5% (9/24), 18.8% (3/16), 16.3% (7/43) and 13.9% (11/79) in endogenous LH surge, GnRH-a, hCG 5,000 IU and hCG 10,000 IU treatment groups, respectively. Conclusion: These results support the concept that use of natural-endogenous LH surge in stimulated cycles may be more effective to obtain pregnancies by IUI than GnRH-a or hCG administration.

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The Dose-Dependent Effects of Nitric Oxide on Human Sperm Cell Function (Nitric Oxide가 인간 정자세포의 기능에 미치는 효과에 대한 연구)

  • Joo, Bo-Sun;Moon, Hwa-Sook;Park, Sue-Jin;Moon, Jae-Yeoun; Cho, Jae-Dong;Kim, Han-Do
    • Clinical and Experimental Reproductive Medicine
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    • v.25 no.1
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    • pp.43-50
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    • 1998
  • This study was performed to determine the effects of nitric oxide on human sperm cell function. Semen samples were obtained from normal healthy volunteers. Motile spermatozoas collected by swim-up method were incubated up to 24 hours in Ham's F-10 medium supplemented with a various concentration of sodium nitroprusside (nitric oxide releasing agent). Sperm motility, hyperactivation, acrosome reaction rate, and acrosin activity were determined. The results are as follows; 1. 1mM of SNP resulted in a significant decrease in sperm motility ($44.8%{\pm}8.9%:78.1%{\pm}6.3%$, and hyperactivation $(10.4%{\pm}6.4%:47.7%:{\pm}9.5%)$ after incubation for 3 hours compared with the control group (Ham's F-10 alone), but had no effect on acrosome reaction. 2. At $100{\mu}M$ SNP, sperm motility was reduced after incubation for 6 hours $(54.8%{\pm}3.2%)$ compared with that of the control group $(82.7%{\pm}8.9%)$, but hyperactivation and acrosome reaction were not affected. 3. However, a lower concentration (less than $10{\mu}M$) of SNP had no effect on sperm motility and hyperactivation for 8 hours of incubation but significantly decreased them when incubation periods were increased up to 24 hours compared with the control group. On the other hand, $1{\mu}M$ and $10{\mu}M$ SNP significantly increased the acrosome reaction rate in both acrosomal status ($17.3%{\pm}5.2%$, $23.5%{\pm}4.7%$, respectively) and acrosin activity ($34.3{\mu}IU{\pm}10.5{\mu}IU,\;45.6{\mu}IU{\pm}5.6{\mu}IU$, respectively) as compared with the control group $(7.0%{\pm}4.0%,\;9.5{\mu}IU{\pm}3.4{\mu}IU)$. These results indicate that SNP, NO releasing agent, has a dose-dependent effects on the sperm cell function. Therefore it may positively affect the fertilization by promoting acrosomal reaction at a lower concentration (less than $10{\mu}M$).

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The association of Osteoporosis and Thyroid Hormone in euthyroid adults (갑상선기능이 정상인 성인에서 골다공증과 갑상선호르몬의 관련성)

  • Yoon, Hyun;Ryu, Eun-Jin
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.2
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    • pp.1137-1144
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    • 2015
  • This study examined the impact of osteoporosis on thyroid hormone in health check-up examinees. The study subjects were 1,117 adults, 20 years and over (636 males, 481 females), who underwent a health package check-up at the general hospital G from January to December, 2011. After adjusting for factors, such as year and gender, the mean thyroid stimulating hormone increased with decreasing T-score (Normal[${\geq}-1g/cm^2$], $1.61{\pm}0.07{\mu}IU/m{\ell}$ and osteopenia[-1 >, ${\geq}-2.5g/cm^2$],$1.82{\pm}0.08{\mu}IU/m{\ell}$ and osteoporosis[< $-2.5g/cm^2$],$3.14{\pm}0.27{\mu}IU/m{\ell}$). After adjusting for factors, such as gender and FBS, the mean free thyroxine decreased with decreasing T-score(Normal, $1.30{\pm}0.01ng/d{\ell}$, and osteopenia, $1.22{\pm}0.01ng/d{\ell}$, and osteoporosis, $1.13{\pm}0.04ng/d{\ell}$). Conclusion. These results suggest that a decrease in T-score might increase the thyroid stimulating hormone and decrease the free thyroxine levelin euthyroid adults.

Study on the Interaction between Vitamins A and E on Their Transfer from Diet to Chicken Eggs, and Effect of Flood-dosing of Dietary Vitamin A on its Content in Eggs and Livers (사료내 비타민 A와 E의 계란내 이행시 상호작용과 비타민 A의 다량투여에 따른 계란 및 간내 함량 변화)

  • 강경래;이창환;남기택;강창원
    • Korean Journal of Poultry Science
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    • v.21 no.4
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    • pp.227-237
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    • 1994
  • This study was conducted to investigate the interaction of vitamins A and E on their transfer from diet to chicken eggs and the effect of vitamin A flood-dosing on its concentration in eggs and livers. In Experiment I, forty-two 45-wk-old brown layers (Bobeock) were divided into seven groups and fed one of seven diets: control, three vitamin A supplemented diets(8, OOO, 16, 000, and 64, 000 IU /kg diet) or three vitamin E supplemented diets (50, 100, and 200 IU/kg diet). In Experiment II, a total of thirty-two 35-wk-old white layers (Hy-ine) were divided into four groups and fed one of four diets :control, vitamin A 20, 00O+vitamin E 200 TU /kg, vitamin A 50, O00+vitamin E 200 IU /kg or vitamin A 100, OO0+vitamin E 200 lU/kg supplemented diets. In Experiment III, a total of fifty-six 35-wk-old white layers (Hy-line) was divided into four groups and fed one of four diets: control or three vitamin A supplemented diets (80, 000, 120, 000 and 160, 000 lU/kg diet). In Experiment I, vitamin E levels of egg yolk in hens fed the vitamin A supplemented diets decreased as dietary vitamin A level increased (P

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Pre-and Post-ishemic Changes of the Constituent Enzymes in Isolated Rabbit's Myocardium (허혈전후 적출 가토 심근내의 구성 효소의 변화)

  • 천수봉;전도환;이재성;김송명
    • Journal of Chest Surgery
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    • v.33 no.2
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    • pp.117-124
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    • 2000
  • Background: Nucleoside transport inhibitor(NTI) Keeps AMP, ADP, ATP levels high in myocytes by inhibiting adenosine cataboilsm so that it may preserve the myocardial contractability during ischemia In this study we investigated the effects of cyclic AMP phosphodiesterase inhibor(C-AMP PDSI) and S-P-nitrobenzyl-6 -thioniosine(NBT; a sort of NIT) on myocadial preservation and changes of constituent enzyme. Material and method: Twenty-six isolated rabbit hearts were perfused with Krebs-Henseleit buffer solution for 20 minutes arrested for 20 minutes and ten reperfused for 30 minutes. The following four groups were prepared and hemodynamic changes coronary effluent lactate dehydrogenase (LDH) a-hydroxybutylic accid(a-HBD) levels and myocardial LDH creatine kinase-MB (CK-MB) adenosine deaminase(ADA) a-HBD levels and myocardial LDH creatine kinase-MB (CK-MB) adenosine deaminase(ADA) a-HBD levels were analysed before and after cardiac arest ; Group I(control) ; the heart was only perfused with K-H ; Group II ; the heart was perfused with K-H including C-AMP PDSI(Amrinone 25mg/L); Group III ; the heart was perfused with K-H including NBT(4.19mg/L) ; Group IV ; the heart was perfused with K-H including C-AMP PDSI + NBT. Result : Left venticular developed pressure(LVDP) at 10 minutes of the equilibrium was significantly higher in group III(72.1$\pm$5.3 mmHg p<0.01) and group III(72$\pm$5.6 mmHg P<0.025) as compared with group I (40.8$\pm$4.7mmHg) and LVDP at 20 minutes of the reperfusion was significantly higher in group II(74$\pm$5.3mmHg p<0.01) and group III(72$\pm$5.6mmHg p<0.025) as compared with group I (44.2$\pm$4.6mmHg). Percentage recovery of LVDP at the reperfusion was the highest in group II(123.3%) Percentage recovery of coronary flow at the equilibrium reperfusion were higher in group II(310%, 270%) group III(230%, 290%) group IV(310%, 280%) as compared with group I (100%) respectively. Myocadial LDH level was significant lower in group IV(33495$\pm$1802 IU/gm p<0.04) as compared with group I(48767$\pm$1421 IU/gm) Myocadial CK-MB level was significant higher in group II(74820$\pm$1421 IU/gm) compared with group I (45450$\pm$1737 IU/gm) Myocadial ADA level was significant higher group IV(1215$\pm$8 IU/gm p<0.05) compared with group I(125$\pm$15 IU/gm) but there was no significant difference between group I and group II ,III, IV in changes of coronary effluent LDH, a-HBD levels. Conclusion: C-AMP PDSI solely appears to have a better effect on myocardial preservation after ischemia than NBT but with no synergistic effect and it could keep CK-MB leve high in myocardial tissues.

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Studies on the Serum Total Creatine Phosphokinase (CPK) Activities and CPK Isosenzymes Fractions of Chicken (닭의 혈청 Creatine phosphokinase (CPK) 총 활성 및 CPK isoenzymes분획에 관한 연구)

  • Kim, Bong-Sik;Hurh, In;Kim, Jong-Hyung;Jang, Myong-Whan;Kim, Won-Sun;Kim, Duk-Whan
    • Korean Journal of Veterinary Service
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    • v.16 no.2
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    • pp.120-126
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    • 1993
  • Clinically healthy 66 female Maniker breed chicken(22 of 2-week-old : group A, 23 of 8-week-old: group B and 21 of 27-week-old: group C) and 66 female Manina breed chicken (21 of 2-week-old : group D, 22 of 8-week-old : group E and 23 of 27-week-old : group F) were examined to establish physiological basic data on serum total Creatine phosphokinase (CPK) activities and CPK isoenzymes fractions. The results obtained were summarized as follows; 1. Serum total CPK activities were $1,088{\pm}254.0\;IU\;/{\ell},$ $1,454{\pm}337.2\;IU\;/{\ell}$ and $1,440{\pm}526.3\;IU\;/{\ell}$ in gorup A, group B and group C of Maniker breed, respectively. Group B and group C showed higher values than that of group A (P<0.01) meaning high values nth aging. 2. Serum total CPK activities were $1,676{\pm}420.5\;IU\;/ {\ell},$ $1,007{\pm}283.1\;IU\;/{\ell}$ and $862{\pm}294.5\;IU/{\ell}$ in group D, group E and group F of Manina breed, respectively. Group D showed the highest value among groups (P<0.01) and Manina breed showed the low values of serum total CPK activities with aging. 3. Manina breed at 2-week-old and Maniker breed at 8-week-old and 27-week-old showed significant high values of total serum CPK activities in breed differance (P<0.01) 4. In the pattern of serum CPK isoenzyme fractions, group A and group B were high with decreasing order of $CK_3>CK_2>CK_1$ and group C was high with decreasing order of $CK_2> CK_3>CK_1$ Groihp D, E and F showed the same pattern with decreasing order of $CK_2>CK_3> CK_1.$ 5. Significance of CPK isoenzymes fractions in breed differance was only found at 8-week-old. $CK_1\;and \;CK_3$ in Maniker breed (P<0.05), and $CK_2$ in Manina breed were higher than that of the other breed (P < 0.01).

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The Clinical Investigation Study of Pancreatitis Developed in Burn Patients (화상환자에서 발생한 췌장염의 임상적 고찰)

  • Cho, Gi Yuon;Chun, Jin Woo;Kim, Young Min;Yoon, Jae Chul;Yim, Haejun;Cho, Yong Suk;Kim, Dohern;Hur, Jun;Chun, Wook
    • Journal of the Korean Burn Society
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    • v.22 no.1
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    • pp.10-14
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    • 2019
  • Purpose: To find progression and prognosis of pancreatitis developed in massive burn patients through retrospective analysis. Methods: A retrospective study was conducted on 32 patients with abnormal increase of serum lipase level among 2523 acute burn patients admitted to our burn center from January 1, 2017 to June 30, 2018. Pancreatitis in this study was defined as a serum lipase concentration level that is higher than 180 IU/L which is three times more than the normal level (less than 60 IU/L). In this study, a retrospective analysis was performed on patients with serum lipase level higher than 300 IU/L to better understand causality of burns and pancreatitis. Results: 32 patients (1.27%) had serum lipase level higher than 180 IU/L among 2523 acute burn subjects. And 13 patients (0.52%) of these 32 patients had serum lipase level elevated more than 300 IU/L. The study indicated serum lipase level was increased around 7 days after the injury. It returned to normal level early as after 1 to 2 weeks and late as after 4 to 6 weeks of injury. The serum amylase level was increased as similar modality as to the serum lipase level increase. The serum bilirubin, AST, ALT, LD, and GGT were also observed to be elevated when serum lipase was more than 1000 IU/L. Conclusion: The pancreatitis developed in burn patients are mostly as mild symptom. It could due to the ischemic injury and can easily be treated by a temporary fasting, TPN, and Gabexate intravenous injection.