• Korean Journal of Food Science and Technology
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• v.44 no.4
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• pp.467-471
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• 2012

This study was conducted to evaluate the bactericidal activity of weakly alkalic electrolyzed water (WEW) against Salmonella Typhimurium and Staphylococcus aureus on perilla leaves. The influences of organic matter, inform of bovine serum, and the ratio of WEW to perilla leaves on bactericidal activity of WEW were also examined. Treatment of these organisms with 25, 50, 75, and 100 ppm WEW was performed for 1 min, 3 min and 5 min, respectively. Higher bactericidal activity was observed after a treatment with 100 ppm WEW compared to a treatment with 25 ppm WEW by 0.7 $log_{10}CFU/g$. The bactericidal activity of WEW also decreased with increasing bovine serum concentration. At the ratio of 10:1 (WEW: perilla leave), levels of Salmonella Typhimurium and Staphylococcus aureus were only reduced by 0.57 and 0.79 $log_{10}CFU/g$, respectively. It is suggested that the removal of organic debris prior to application of sanitizers and treatment above the ratio of 25:1 (WEW: perilla leave) is needed in order to improve WEW activity.

• Korean Journal of Food Science and Technology
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• v.42 no.6
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• pp.739-742
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• 2010

In order to select a superior yeast, 8 kinds of commercial active dry yeasts (Lalvin 1116, Lalvin 1118, Lalvin D-47, Lalvin Bourgovin, Parisienne, Fermivin, Red Star Monrachet, and Red Star Premier Cuvee) were utilized for omija wine fermentation. During fermentation, the physicochemical characteristics and sensory properties of the various omija wines were evaluated. According to the results, pH and titratable acidity were in ranges of 3.0-3.3% and 1.8-2.4%, respectively. Sugar content was $24^{\circ}Bx$ at early fermentation and changed to $8.4-10.2^{\circ}Bx$ at 24 days of fermentation. While the omija wines fermented by Lalvin D-47 and Red Star Premier Cuvee showed the highest alcohol contents (13.0%), the omija wine fermented by Parisienne showed the lowest alcohol content (10.8%). The omija wine fermented by Lalvin 1118 had an alcohol content of 12.0% and showed the lowest yeast count of 5.8 log CFU/mL. Hunter's values (L, a, and b) were all different among the 8 omija wines. Moreover, the omija wine fermented by Lalvin 1118 showed the highest scores for taste ($6.75{\pm}1.68$), swallowing ($6.65{\pm}1.50$), and overall acceptability ($6.70{\pm}1.34$). It is concluded that Lalvin 1118 was the best yeast among 8 tested commercial active dry yeasts, having a high potential for omija wine fermentation.

• Journal of Life Science
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• v.16 no.4
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• pp.632-639
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• 2006

Here we examined the expression patterns and regulation of seven photosynthesis (PS) genes (puf, puc, puhA, bchC, bchE, bchF, and bchI) in the anoxygenic photosynthetic bacterium, Rhodobacter sphaeroides, based on lacZ reporter gene assay. Expression of the tested PS genes, except puhA and bchI, were strongly induced in R. sphaeroides grown under anaerobic conditions relative to that under aerobic conditions. The puhA and bchI genes appear to form the operons together with bchFNBHLM-RSP0290 and crtA, respectively. Expression of the puf, puc, and bchCXYZ operons in R. sphaeroides grown photosynthetically was proportional to the incident light intensity, whereas that of bchFNBHLM(RSP0290-puhA) was inversely related to light intensity. Expression of bchEJG was lowest under medium-light photosynthetic conditions $(10\;W/m^2)$ and highest under high light conditions $(100\;W/m^2)$. The regulation of PS genes by the three major regulatory systems involved in oxygen- and light-sensing in R. sphaeroides is as following: puf and bchC are regulated by both the PpsR repressor and the PrrBA two-component system. The puc operon is under control of PpsR, FnrL, and PrrBA system. Expression of bchE is controlled by FnrL and PrrBA two-component system, whereas bchF is regulated exclusively by PpsR. It was demonstrated that the PpsR repressor is responsible for high-light repression of bchF and that FnrL might be involved in perceiving the cellular redox state in addition to sensing $O_2$ itself.

• Journal of Life Science
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• v.19 no.7
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• pp.983-993
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• 2009

The effects of Monascus-fermented Korean red ginseng (MFRG) on the contents of serum lipids and tissues lipid peroxidation was investigated in alcohol feeding rats (AC group). Serum contents of total lipid and free fatty acid in alcohol feeding rats were significantly increased, but these increases tended to decrease in the AMFRG group. Serum triglyceride content was also significantly decreased in the AMFRG group compared to other groups. Serum content of total-cholesterol was significantly increased in AC group compared to normal control (NC) group, whereas there was no significant difference between the AC and AMFRG groups. Content of HDL-cholesterol in serum was slightly increased in the AC group compared to the NC group, but this increase in the AC group was more significantly increased in the AMFRG group. At the same time, atherogenic index (AI) was also significantly decreased in the AMFRG group compared to the AC group. Contents of thiobarbituric acid reactive substances (TBARS) in the liver, heart, spleen and testes were significantly increased in the AC group compared to the NC group, but these increases were significantly less in the AMFRG group. Contents of liver nonheme ion was increased in the AC group and was significantly decreased in the AMFRG group, which suggested that lipid peroxidation contents are inversely correlated with liver nonheme ion content. Hepatic glutathione concentration was significantly decreased in the AC group, but this content was significantly increased in the AMFRG group and it showed the antioxidant abilities of glutathione. These results suggested that Monascus-fermented Korea red ginseng has anti-atherogenic index (AI) effects as well as antioxidative activities through reduced tissue oxidative stress in alcohol feeding rats.

• Journal of Life Science
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• v.24 no.6
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• pp.618-625
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• 2014

Rice flour is used in many food products. However, dough made from rice lacks extensibility and elasticity, making it less suitable than wheat for many food products such as bread and noodles. The high-molecular weight glutenin subunits (HMW-GS) of wheat play a crucial role in determining the processing properties of the wheat grain. This paper describes the development of marker-free transgenic rice plants expressing a wheat Glu-Dy10 gene encoding the HMG-GS from the Korean wheat cultivar 'Jokyeong' using Agrobacterium-mediated co-transformation. Two expression cassettes, consisting of separate DNA fragments containing Glu-1Dy10 and hygromycin phosphotransferase II (HPTII) resistance genes, were introduced separately into Agrobacterium tumefaciens EHA105 for co-infection. Each EHA105 strain harboring Glu-1Dy10 or HPTII was infected into rice calli at a 3: 1 ratio of Glu-1Bx7 and HPTII. Among 290 hygromycin-resistant $T_0$ plants, we obtained 29 transgenic lines with both the Glu-1Dy10 and HPTII genes inserted into the rice genome. We reconfirmed the integration of the Glu-1Dy10 gene into the rice genome by Southern blot analysis. Transcripts and proteins of the Glu-1Dy10 in transgenic rice seeds were examined by semi-quantitative RT-PCR and Western blot analysis. The marker-free plants containing only the Glu-1Dy10 gene were successfully screened in the $T_1$ generation.

• Korean Journal of Agricultural and Forest Meteorology
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• v.18 no.4
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• pp.307-319
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• 2016

A Land-Atmosphere Modeling Package (LAMP) for supporting agricultural and forest management was developed at the National Center for AgroMeteorology (NCAM). The package is comprised of two components; one is the Weather Research and Forecasting modeling system (WRF) coupled with Noah-Multiparameterization options (Noah-MP) Land Surface Model (LSM) and the other is an offline one-dimensional LSM. The objective of this paper is to briefly describe the two components of the NCAM-LAMP and to evaluate their initial performance. The coupled WRF/Noah-MP system is configured with a parent domain over East Asia and three nested domains with a finest horizontal grid size of 810 m. The innermost domain covers two Gwangneung deciduous and coniferous KoFlux sites (GDK and GCK). The model is integrated for about 8 days with the initial and boundary conditions taken from the National Centers for Environmental Prediction (NCEP) Final Analysis (FNL) data. The verification variables are 2-m air temperature, 10-m wind, 2-m humidity, and surface precipitation for the WRF/Noah-MP coupled system. Skill scores are calculated for each domain and two dynamic vegetation options using the difference between the observed data from the Korea Meteorological Administration (KMA) and the simulated data from the WRF/Noah-MP coupled system. The accuracy of precipitation simulation is examined using a contingency table that is made up of the Probability of Detection (POD) and the Equitable Threat Score (ETS). The standalone LSM simulation is conducted for one year with the original settings and is compared with the KoFlux site observation for net radiation, sensible heat flux, latent heat flux, and soil moisture variables. According to results, the innermost domain (810 m resolution) among all domains showed the minimum root mean square error for 2-m air temperature, 10-m wind, and 2-m humidity. Turning on the dynamic vegetation had a tendency of reducing 10-m wind simulation errors in all domains. The first nested domain (7,290 m resolution) showed the highest precipitation score, but showed little advantage compared with using the dynamic vegetation. On the other hand, the offline one-dimensional Noah-MP LSM simulation captured the site observed pattern and magnitude of radiative fluxes and soil moisture, and it left room for further improvement through supplementing the model input of leaf area index and finding a proper combination of model physics.

• Journal of Preventive Medicine and Public Health
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• v.21 no.2 s.24
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• pp.307-319
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• 1988

In order to determine the contents of lead, cadmium and zinc, the tissues of the crussian carp, Carassius carassius, collected from the middle streams of the Nakdong river were examined. During the dry season from 8 to 15, March, 1987, six loci were selected to sample the fish and river water; five of them were the midstreams of the Nakdong river i. e., the vicinities of the Andong dam, the Nakdong bridge, the Waegwan bridge, Gangjung and Gaepori, the other one was the Kumho river around the Paldal bridge(see Fig. 1). The microanalyses of lead and cadmium contents were undertaken by the atomic absorption spectrophotometer(Model IL-551) connected with CTF-IL 655, while that of Zinc by the flame method with IL551 only. The contents of lead and cadmium in water sampled from Gaepori distant from about 30km downward from the junction with the Kumho river were about 1.5 times higher than those from Gangjung distant about 0.5km upward from the junction, and the content of zinc from Gaepori was slightly higher than that from Gangjung. However, the contents of lead and cadmium in water sampled from Gaepori were three fifth of those from the vicinity of Paldal bridge far about 2km upperward from the junction. In the other words, the contents of lead and cadmium in the samples from the vicinity of the Paldal bridge were about 2.5 to 3 times higher than those from the Gangjung and 5 times higher than those from the Andong dam. The contents of the heavy metals in the tissues of Carassius carassius were relatively consistent to those of the aquatic environments where the fish were collected, with higher contents in the tissues of the crussian carp collected from the vicinity of Gaepori and the Paldal bridge which were so much polluted than in those from other loci in upper stream from the junction. And the contents of lead and cadmium in tissues were positively correlated with the age of the fish, however, that of zinc was not. The contents of lead in bone of crussian carp from the vicinity of the Paldal bridge in the group of 0-1 year old fish were similar to those of 4-5 years old fish from Gaepori, and higher than those of 3-4 years old fish collected from the upper stream of the junction. It is likely that fishing from Gaepori and the paldal bridge is not recommended, and all the industrial producers have to pay intensive attention to the water pollution due to the sewage disposal.

• Korean Journal of Weed Science
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• v.16 no.3
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• pp.187-193
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• 1996

To supply seeds with a good quality as plant materials for herbicide screening and to know whether the germination characteristics could be associated with a differential response to herbicides, germination characteristics and differential responses to herbicides were investigated with 3 species of a genus Setaria ; Yellow foxtail(Setaria glauca P. Beauv, SETGL), Giant foxtail(Setaria faberi Hetrm, SETFA), and Green foxtail(Setaria viridis P. Beauv, SETVI). Degree of dormancy was high in the order of SETGL, SETVI and SETFA. The dormancy of SETGL seed was relatively well removed by room temperature and drying storage, but SETFA and SETVI by low temperature and wetting storage(stratification). For breaking dormancy of SETGL, SETVI and SETFA, it was necessary for being kept under the above storage conditions for at least 2, 4 and 4-5 months, respectively. When the dormancy-breaked seeds were transfered to low temperature($4^{\circ}C$) and drying condition, SETGL showed germination rate of 96% even after 2 month storage. However, SETVI and SETFA showed a decreased germination of 54% and 69%, respectively, with a decreased velocity of germination, indicating that secondary dormancy might be induced. On the other hand, a significant change in germination rate was not observed as the seeds were transfered to room temperature($25^{\circ}C$) and drying condition. The germinability of SETGL seed began to decline from 6th year after storage in room temperature and drying condition. All of 3 species showed relatively high germination rate at alternating temperature of $30^{\circ}C$/$20^{\circ}C$(14hr/10hr) and their germination were not increased by light. All of 3 species exhibited similar responses to cycloxydim, sethoxydim and primisulfuron in greenhouse experiment. In contrast, SETVI and SETFA were relatively susceptible to fenoxapropethyl, SETFA to fluazifop-butyl, SETGL and SETFA to clorimuron-ethyl, and SETGL to EK-2612. The difference in herbicidal response among 3 species was the highest in the treatment of EK-2612. These results suggest that there is no a consistent tendency in responses of 3 species to herbicides which have the same target site. And the relationship between germination characteristics and differential responses to herbicides was not found.

• Korean Journal of Weed Science
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• v.16 no.3
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• pp.200-209
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• 1996

It is assumed to be an efficient method for keeping a germinability of weed seeds as long as possible, if a secondary dormancy is not induced by transferring the seeds of which dormancy was broken in wetting condition into drying condition. To investigate its validity, two experiments were carried out on seeds of 9 weed species ; to find out the most effective storage condition in breaking the dormancy of each weed species and to know whether there is a decrease in the germinability by transferring into drying storage condition. The dormancy of Chenopodium album and Stellaria aquatica was released well under the drying condition, but that of Echinochloa crus-galli var. oryzicola by soaking in water. Other weed species were released from dormancy by storage in wetting condition. When the seeds stored in the wetting or soaking condition, are air-dried and then restored at room or low temperature, a decreasing tendency of germinability which might cause a trouble in using them practically, was not observed except on the seeds of Persicaria vulgaris. In the case of Persicaria vulgaris, the low germination since 3 month-storage seemed not to be caused by drying, because a decrease of its germinability was observed with increasing storage period in all of the storage conditions. In contrast, high germination was induced as the seeds of Echinochloa crusgalli var. oryzicola, which were not germinated during the storage in low temperature and wetting condition, were transferred into the room temperature and drying condition. These results suggest that this approach can be one of the efficient methods for keeping a good germinability as long as possible in most weed seeds.

• Korean Journal of Weed Science
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• v.16 no.3
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• pp.210-220
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• 1996

Ten-day-old seedlings of the oxyfluorfen-tolerant and -susceptible rice cultivars with barnyardgrass, a typical susceptible weed were soaked in oxyfluorfen at $10^{-6}M$ for 2 hrs kept for 24 hrs in the dark, and exposed to light for 0, 2, 4, or 6 hrs to investigate changes in the activity and isozyme of antioxidative enzymes. The activities of antioxidative enzymes of APOX, CAL, POX, NR, GR, MDAR and SOD in the tolerant and susceptible rice cultivate themselves didn't show any difference but the activity in the susceptible barnyardgrass was very low in comparison with rice cultivars. The activity of lipoxygenase tended to be some slightly higher in the susceptible rice cultivars and barnyardgrass than in the tolerant rice cultivars. The activities of MDAR, POX, GR and SOD, antioxidative enzymes, were higher in the tolerant rice cultivars than in the susceptible rice cultivars and barnyardgrass after the treatment of oxyfluorfen. After the treatment of oxyfluorfen, in the change of POX isozyme, the activity of C band in the tolerant rice cultivars increased with increased concentration but it didn't in the susceptible rice cultivar. The activity of B band decreased slightly at $10^{-4}M$ in the susceptible barnyardgrass. Isozyme of GR, SOD and AO by the treatment of oxyfluorfen, the activity of each band between the tolerant and susceptible rice cultivars showed no difference but GR isozyme C band was disappeared in the susceptible barnyardgrass at $10^{-4}M$. In the change of esterase isozyme resulting from the treatment of oxyfluorfen, the activities of B, C and D bands decreased more in the susceptible rice cultivars than in tolerant rice cultivars, and A band was disappeared in the susceptible barnyardgrass at $10^{-4}M$.