• Plant Biotechnology Reports
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• v.2 no.3
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• pp.191-198
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• 2008

Germplasm characterization and evolutionary process in viable populations are important links between the conservation and utilization of plant genetic resources. Here, an investigation is made, based on molecular and biochemical techniques for assessing and exploiting the genetic variability in germplasm characterization of taro, which would be useful in plant breeding and ex situ conservation of taro plant genetic resources. Geographical differentiation and phylogenetic relationships of Indian taro, Colocasia esculenta (L.) Schott, were analyzed by random amplified polymorphic DNA (RAPD) and isozyme of seven enzyme systems with specific reference to the Muktakeshi accession, which has been to be proved resistant to taro leaf blight caused by P. colocasiae. The significant differentiations in Indian taro cultivars were clearly demonstrated by RAPD and isozyme analysis. RAPD markers showed higher values for genetic differentiation among taro cultivars and lower coefficient of variation than those obtained from isozymes. Genetic differentiation was evident in the taro accessions collected from different regions of India. It appears that when taro cultivation was introduced to a new area, only a small fraction of genetic variability in heterogeneous taro populations was transferred, possibly causing random differentiation among locally adapted taro populations. The selected primers will be useful for future genetic analysis and provide taro breeders with a genetic basis for selection of parents for crop improvement. Polymorphic markers identified in the DNA fingerprinting study will be useful for screening a segregating population, which is being generated in our laboratory aimed at developing a taro genetic linkage map.

• Journal of Korean Physical Therapy Science
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• v.11 no.4
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• pp.5-10
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• 2004

This study selected 10 university football male players and repeatedly treated them as sports massage group, exercise resumption group, and stability resumption. After maximum exercise, when sport massage, exercise resumption, and stability resumption were applied during recovery, blood lactic acid concentration and $LDH_5$ isozyme concentration were examined. Finally this study obtains the following conclusion 1. There was a significant difference in both lactic acid concentration and time between groups. Besides, the cross-action on group and time was significant. 2. There was a significant difference in both $LDH_5$ concentration and time between groups. Besides, the cross-action on group and time was significant. Based on the above findings, this study suggests that sport massage resumption and exercise resumption remove blood lactic acid more quickly than stability resumption to promote recovery. That means that sport massage and exercise resumption anre very effective for lowering the level of fatigue. Thus sport massage or exercise resumption treatment is considered to have a very positive effect on player's quick recovery and it should be used aggressively in a field.

• Journal of Preventive Medicine and Public Health
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• v.3 no.1
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• pp.111-119
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• 1970

Alterations of H-and M-isozymes of Lactic Dehydrogenase(LDH) were observed in the various tissues after exposing the rats to 50ppm and 250ppm of sulfur dioxide. These isozymes of the respective tissue were separated by Diethlaminoethyl (DTAE)-cellulose from the tissue homogenates of brain, lung and muscle, presenting the activities by rate of reduction of nicotinamide-adenine-dinucleotide ($NAD^+$). Pure LDH and the coenzyme ($NAD^+$) were directly treated with sulfur dioxide in vitro in order to find out the direct to sulfur dioxide on LDH and $NAD^+$ and the results were as follows. 1. In the normal tissues, the H-isozyme activity was dominant in the brain and heart, and the M-isozyme in the muscle. 2. In the lung tissue of normal rats, there was no difference between the activity of H-and M-type of LDH. 3. When rats inhale sulfur dioxide gas in concentration of 50ppm and 250ppm, it appeared that the H-type tend to be suppressed in aerobic tissues and the M-type in anaerobic tissues. 4. In the lung tissue exposed to sulfur dioxide, both the LDH activities were suppressed. 5. It seems that LDH and the coenzyme ($NAD^+$) are not directly affected by exposing in sulfur dioxide gas.

• Korean journal of applied entomology
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• v.29 no.3
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• pp.170-175
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• 1990

These studies were conducted to examine the mechanism of acaricidal resistance in the twospotted spider mite (Tetranychus urticae Koch). The resistant strains were obtained by succssive selection of five acaricides including carbonphenothion and ethion of organophosphorus compound, dicofol of organochlorine compound, cyhexatin of compound and biphenthrin of synthetic pyrethroid. Esterase isozymes were separated by polyacrlyamide gel susceptible strains. The differences of the esterase isozymes of the resistant strains were Est. 1, Est. 3 in the carbonphenothion-selected strain, Est. 3 in the ethion- and the cyhexatin-selected strains, Est. 1, Est. 3, Est. 7 in the dicofol-selected strain, Est. 7 in the biphenthrin-selected strain as compared to the susceptible strain. With the difference of electrophoretic bands and their activities, esterases were related to the resistant mechanism of tested acaricides.

• Korean Journal of Plant Resources
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• v.10 no.3
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• pp.235-240
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• 1997

To study the effects of metal ions on the activity of anti-oxidase enzymes, the activity of superoxide dismutase (SOD) and peroxidase (POD) and isozyme patterns of Brassica juncea have been studied after treating with CD, Cu, Zn, and Al. The activity of SOD after treating with metal ions was higher than that of untreated control. SOD activity in leaves increased by treatment of 50 ppm of Zn and 500 ppm of Al. POD in stems gave highest activity after treating with 500 ppm of Cu. When the activity was compared by plant parts, lowest POD activity was observed in leaves in which protein content was higher than other tissues. When the activity was expressed as percentage of control, SOD activity was increased after treating with metal ions. SOD activity in leaves and roots of metal treated plant was significantly increased under the metal ions stress conditions. In the roots of 50 ppm of Zn treated plant, SOD activity was extremly high. POD activity was inhibited with Cd and Zn treatment in all parts of the plant. However, in leaves and stems, there was marked increase in activity after treating with Cu. The patterns of SOD isozyme after metal treatment show that two bands were stained in all metal ion treated and that no new band appeared. POD isozyme band intensity resulting from the treatment of metal ions was in order of roots > stems > leaves, but there was no significant difference.

• The Korean Journal of Mycology
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• v.16 no.2
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• pp.95-100
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• 1988

Isozyme band patterns were investigated by isoeletric focusing Esterase and Leucine amino peptidase of Pleurotus spp. in Korea. Esterase patterns of mycelia and fruitbody were distinquished. However, those of primordia, cap and stem were similar. Interspecies differences of Pleurotus ostreatus, P. cornucopiae and P. florida of Esterase zymogram were found. Species identification by electrophoretic zymogram may be a role as an additional taxonomic tool.

• The Korean Journal of Zoology
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• v.23 no.4
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• pp.263-272
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• 1980

Polyacrylamide gel electrophoresis was used to investigate the patterns of LDH and MDH isozymes in the embryo and adult of amphibia; Rana nigromaculata, Rana plancyi chosenica and Hynobius leechii. Rana nigromaculata is considered to be heterozygous for the gene specifying the "B" subunit of LDH, and Hynobius leechii to be heterozygous for the gene specifying the "A" subunit of LDH. The LDH isozyme paatern of embryos of the above three species is characterized by a gradual increase in the activity of LDH-5 (muscular form)during development. Two or three molecular forms of MDH is present steadily from early embryos and in adult. Of the MDH isozymes, the more cathodic one (MDH-m) appears weakly in early developing stages, but increases slowly in the activity as the embryo develops.the embryo develops.

• The Korean Journal of Zoology
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• v.19 no.2
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• pp.79-84
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• 1976

$M_4$-LDH isozyme was partially purified from the skeletal muscle of Agkistrodon blomhoffii brevicaudus. The protein was injected into rabbits and the resulting antiserum was tested for reactivity with crude preparations of LDH isozymes of fifteen vertebrate species. Antisera against $M_4$-LDH isozyme of A. blomhoffii brevicaudus reacted very strongly with the LDH isozymes, except the $H_4$-LDH isozyme, of A. saxatilis and A. caliginosus but weakly with those of Rhabdophis tigrinus at fixed conditions. A. caliginosus showed a difference in the immunodiffusion test and was considered to be a species less related to others of genus Agkisrodon. The suggestion that the H and M lactate dehydrogenase subunits are immunclogically distinct has been reaffirmed in the present study.

• YAKHAK HOEJI
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• v.13 no.4
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• pp.101-110
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• 1969

The isozyme alteration of lactic dehydrogenase in the tissues of albino rat inhaled SO$_{2}$ were studied in vivo and in vitro, with the following results: (1) The H-type of LDH activity relatively dominated in the normal brain, heart and kidney tissues of rat, M-type in the normal lung, liver, and muscle tissues of the animal. (2) When rats inhale SO$_{2}$ in the concentration of 250 ppm, it appears that the M-type tends to predominate in the anaerobic tissues such as liver, kidney and muscle tissues and the H-type in the aerobic tissues such as brain and heart tissues. (3) When 5% SO$_{2}$ is introduced into tissue homogenates, LDH activities in the heart, lung, liver and muscle tissues are increased more than that of introducing room-air only. With sam treatment, LDH activity is decreased in the kidney tissue and no alteration is observed in the brain tissue. (4) Although, after the aeration of SO$_{2}$, the oxygen tension seems to bring decreases in the level of LDH activity in the anerobic tissues such as liver and muscle tissues, while, on the other hand, increases in the level of the activity in the aerobic tissues, such as the brain, heart and lung tissues. (5) Accordinglly, SO$_{2}$ affects LDH activities, its isozyme pattern of each organs, and their metabolic pathway by its absorption of the gas.

• Environmental Mutagens and Carcinogens
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• v.9 no.2
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• pp.87-96
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• 1989

Studies on the biodisposition of beta-nicotyrine by lung and liver microsomes was examined in order to provide a better understanding of its fate in this tissue. beta-nicotyrine (100$\mu$M) was incubated with microsomes (1 mg/ml) prepared from New Zealand White rabbits. The rate of oxidation observed in lung microsomal incubations was 1.7 nmoles $\beta$-nicotyrine oxidized mg$^{-1}$ min$^{-1}$ compared with 2.7 nmoles $\beta$-nicotyrine oxidized mg$^{-1}$ min$^{-1}$ by the liver microsomal preparation. However, when these rates were expressed as a function of cytochrome P-450 content, the specific activity of the metabolic oxidation catalyzed by lung (8.3 nmoles $\beta$-nicotyrine oxidized nmole cytochrome P-450$^{-1}$ min$^{-1}$) was approxiamtely 4 times greater than liver microsomes (2.3 nmoles $\beta$-nicotyrine oxidized nmole cytochrome P-450$^{-1}$ min$^{-1}$). Isozyme studies on the oxidation of $\beta$-nicotyrine employed several methods of altering activities of specific isozymes present in pulmonary microsomes, including the use of the isozyme 2 and 6 specific inhibitor $\alpa$-methyl ABT, metabolic inhibitor(MI) complex formation. The results of this inhibition study would appear to indicate the $\beta$-nicotyrine is metabolized predominantly by pulmonary isozyme 5.