• 한국연초학회지
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• 제30권1호
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• pp.14-24
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• 2008

The objective of this study was to determine the effect of smoking conditions on the in vitro toxicological activity of mainstream smoke. The 2R4F reference cigarette was machine-smoked by International Organization for Standardization(ISO) and Canadian Intense(CI) conditions. Smoke was analysed for chemical composition and in vitro toxicity. The cytotoxic potencies of both the total particulate matter(TPM), which were collected in Cambridge filter pad, and gas/vapor phase(GVP), which was bubbled through in phosphate-buffer saline in a gas-washing bottle, were assessed neutral red up take assay with chinese hamster ovary(CHO) cells. The assessment for genotoxicity of TPMs generated under ISO and CI conditions was determined using Salmonella mutagenicity assay and in vitro micronucleus assay. When calculated on an equal TPM basis, in vitro toxicity of TPM obtained under CI condition was decreased compared to TPM generated under ISO condition. The results of chemical composition analyses revealed that the lower toxicological activity under CI condition than that of ISO condition could be explained by the decreased in the contents of phenols, N-nitrosoamines and aromatic amines of TPM on an equal TPM basis.

• 한국연초학회지
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• 제29권2호
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• pp.125-131
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• 2007

Hydrogen cyanide(HCN), formed from pyrolysis of various nitrogenous compounds such as protein, amino acids and nitrate in tobacco, is present in both the particulate phase and vapor phase of cigarette smoke. Typically the determination of HCN in cigarette smoke has been done through colorimetric and electrochemical techniques, such as fluorescence spectrometry, UV-spectrophotometry (UV), continuous flow analyzer (CFA), capillary GC-ECD and ion chromatography (IC). Most of these techniques are known to be time-consuming and some of them lack specificity or sensitivity. The available results from both our laboratory and reported literatures for 2R4F Kentucky reference cigarette, smoked under ISO condition, show a relatively wide variation ranging from 100 to 120 ug/cig of HCN. Especially, the precision and accuracy of the analytical results of HCN tend to get worse in low tar cigarettes and under intense smoking condition. In this paper, a more optimized analytical methods than previous ones are suggested. This method shows lower detection limit and has improved precision and accuracy, so it is applicable for wide tar level cigarettes under intense smoking condition as well as under ISO smoking condition. Important features of this method are improved sample collection and quantification systems such as the number of trapping units, volume, temperature and type of trapping solution. To avoid volatilization loss of HCN in analyzing mainstream smoke, it is highly recommended that pH values of trapping solutions should be maintained over 11 and cold traps should be used in collecting mainstream smoke.

• 한국연초학회지
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• 제33권1호
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• pp.28-38
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• 2011

This experiment was conducted as a part of Asia collaborative study on purpose of verifying the difference between CM6 and CM7 including 3R4F for reference. It carried out using various analytical categories for example, main stream, sidestream and Av. smoke. Additional analysis such as physical properties, blending ratio, combustibility and general leaf component analysis also implemented in order to investigate the difference. We complied with ISO standard and CORESTA recommended method during analytical operating procedures. In this study, we described that comparative analytical result for CM6 and CM7 known as reference or monitoring cigarettes including 3R4F for reference. All sample cigarettes were conditioned at $22^{\circ}C$, 60% relative humidity for 48 hours. Av. Smoke, MS and SS smoke analysis were performed over five times with two smoking condition, ISO and Health Canada with the exception of Av. smoke analysis. We complied with ISO standard method during analytical operating procedures. And, we conducted additional analysis, such as physical properties, blending ratio, combustibility and leaf component analysis also in order to investigate the difference. In conclusion, we found out some differences between CORESTA monitoring cigarette No. 6 and No 7. The smoke components such as total particulate matters, NFDPM, nicotine and carbon monoxide contents of CM7 were a little lower than CM6. And, these phenomena were the same as not only main stream smoke but also side stream smoke and Av. smoke. This tendency was consistent with ISO and Health Canada smoking condition. Besides, leaf constituents' color of CM7 was darker than CM6. In case of combustibility, it showed short combustion time approximately 30 seconds.

• 한국연초학회지
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• 제28권2호
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• pp.136-143
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• 2006

This study was conducted to determine the amount of unprotonated nicotine in cigarette mainstream smoke and to investigate its relationship to level of filter ventilation and machine smoking conditions. Unprotonated nicotine from TPM trapped on a Cambridge filter pad(CFP) was adsorbed by Carboxen/PDMS SPME fiber, thermally desorbed and determined by GC/MS. 2R4F reference cigarette, twelve commercial brands from the Korean market and five test cigarette samples, which had the same tobacco blend with different levels of filter ventilation, were analyzed for unprotonated nicotine. In commercial brands, the amount of unprotonated nicotine changed slightly depending on the pH values of smoke, and decreased as the tar level increased. filter ventilation in these commercial cigarettes was $28{\sim}80%$ and the higher filter ventilation increased relative unprotonated nicotine levels, but not significantly. However, in five test cigarettes with different filter ventilation$(0{\sim}70%)$, unprotonated nicotine levels increased almost linearly with the level of filter ventilation. Concentrations of unprotonated nicotine in mainstream smoke generally increased in order $HC\;<\;ISO\;{\leq}\;MDPH$ machine smoking conditions. The ratio of unprotonated nicotine to total nicotine among $cigarettes({\alpha}_{fb})$ increased in order RC < MDPB < ISO conditions. Concentrations of unprotonated nicotine varied with three machine smoking conditions.

• 한국연초학회지
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• 제31권2호
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• pp.107-114
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• 2009

In this study, we investigated the existence of melamine and cyanuric acid in cigarette smoke because leaf tobacco contains lots of nitrogen compounds and they are capable of pyrolytic synthesis while smoking. The extraction and derivatization conditions of two compounds were performed according to the procedure of U.S. Food and Drug Administration(FDA). Ky3R4F as reference cigarette was smoked on ISO condition(Puff volumne : 35 ml, duration : 60 sec, Interval : 2 sec). Compared with the results Ky3R4F and standard solution, retention time of two compounds was not corresponded. As a result, we concluded that melamine and cyanuric acid were not existed in cigarette smoke.