• Journal of Microbiology
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• 제45권3호
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• pp.227-233
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• 2007

In budding yeast, septin plays as a scaffold to recruits protein components and regulates crucial cellular events including bud site selection, bud morphogenesis, Cdc28 activation pathway, and cytokinesis. Phosphorylation of Bni5 isolated as a suppressor for septin defect is essential to Swe1-dependent regulation of bud morphogenesis and mitotic entry. The mechanism by which Bni5 regulates normal septin function is not completely understood. Here, we provide evidence that Bni5 phosphorylation is important for interaction with septin component Cdc11 and for timely delocalization from septin filament at late mitosis. Phosphorylation-deficient bni5-4A was synthetically lethal with $hof1{\Delta}$. bni5-4A cells had defective structure of septin ring and connected cell morphology, indicative of defects in cytokinesis. Two-hybrid analysis revealed that bni5-4A has a defect in direct interaction with Cdc11 and Cdc12. GFP-tagged bni5-4A was normally localized at mother-bud neck of budded cells before middle of mitosis. In contrast, at large-budded telophase cells, bni5-4A-GFP was defective in localization and disappeared from the neck approximately 2 min earlier than that of wild type, as evidenced by time-lapse analysis. Therefore, earlier delocalization of bni5-4A from septin filament is consistent with phosphorylation-dependent interaction with the septin component. These results suggest that timely de localization of Bni5 by phosphorylation is important for septin function and regulation of cytokinesis.

• 한국기후변화학회지
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• 제9권1호
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• pp.103-115
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• 2018

The uncertainty of climate scenarios, as initial information, is one of the significant factors among uncertainties of climate change impacts and vulnerability assessments. In this sense, the quantification of the uncertainty of climate scenarios is essential to understanding these assessments of impacts and vulnerability for adaptation to climate change. Here we quantified the precision of surface temperature of ensemble scenarios (high resolution (1km) RCP4.5 and 8.5) provided by Korea Meteorological Administration, with spatiotemporal variation of the standard deviation of them. From 2021 to 2050, the annual increase rate of RCP8.5 was higher than that of RCP4.5 while the annual variation of RCP8.5 was lower than that of RCP4.5. The standard deviations of ensemble scenarios are higher in summer and winter, particularly in July and January, when the extreme weather events could occur. In general, the uncertainty of ensemble scenarios in summer were lower than those in winter. In spatial distribution, the standard deviation of ensemble scenarios in Seoul Metropolitan Area is relatively higher than other provinces, while that of Yeongnam area is lower than other provinces. In winter, the standard deviations of ensemble scenarios of RCP4.5 and 8.5 in January are higher than those of December. Especially, the standard deviation of ensemble scenarios is higher in the central regions including Gyeonggi, and Gangwon, where the mean surface temperature is lower than southern regions along with Chungbuk. Such differences in precisions of climate ensemble scenarios imply that those uncertainty information should be taken into account for the implementation of national climate change policy.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제36권6호
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• pp.481-489
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• 2010

Introduction: TLR-5, a member of the toll-like receptor (TLR) family, is a element of the type I transmembrane receptors, which are characterized by an intracellular signaling domain homolog to the interleukin-1 receptor. These receptors recognize microbial components, particularly bacterial flagellin. All-trans retinoic acid (atRA, tretinoin), a natural metabolite of vitamin A, acts as a growth and differentiation factor in many tissues, and is also needed for immune functions. In this study, THP-1 human macrophage-monocytes were used to examine the mechanisms by which atRA regulated the expression of TLR-5. Because the molecular mechanism underlying this regulation at the transcriptional level is also unclear, this study examined which putative transcription factors are responsible for TLR-5 expression by atRA in immune cells. Materials and Methods: This study examined whether atRA induces the expression of TLR-5 in THP-1 cells using reverse transcription-polymerase chain reaction (RT-PCR), and which transcription factors are involved in regulating the TLR-5 promoter in RAW264.7 cells using a reporter assay system. Western blot analysis was used to determine which signal pathway is involved in the expression of TLR-5 in atRA-treated THP-1 cells. Results: atRA at a concentration of 10 nM greatly induced the expression of TLR-5 in THP-1 cells. Human TLR-5 promoter contains three Sp-1/GC binding sites around -50 bp and two NF-kB binding sites at -380 bp and -160 bp from the transcriptional start site of the TLR-5 gene. Sp-1/GC is primarily responsible for the constitutive TLR-5 expression, and may also contribute to NF-kB at -160 bp to induce TLR-5 after atRA stimulation in THP-1 cells. The role of NF-kB in TLR-5 expression was further confirmed by inhibitor pyrrolidine dithiocarbamate (PDTC) experiments, which greatly reduced the TLR-5 transcription by 70-80%. Conclusion: atRA induces the expression of the human TLR-5 gene and NF-kB is a critical transcription factor for the atRA-induced expression of TLR-5. Accordingly, it is conceivable that retinoids are required for adequate innate and adaptive immune responses to agents of infectious diseases. atRA and various synthetic retinoids have been used therapeutically in human diseases, such as leukemia and other cancers due to the antiproliferative and apoptosis inducing effects of retinoids. Therefore, understanding the molecular regulatory mechanism of TLR-5 may assist in the design of alternative strategies for the treatment of infectious diseases, leukemia and cancers.

• 대기
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• 제29권2호
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• pp.149-164
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• 2019

The purpose of this study is to introduce the improvement of current operational climate prediction system of KMA and to compare previous and improved that. Whereas the previous system is based on GloSea5GA3, the improved one is built on GloSea5GC2. GloSea5GC2 is a fully coupled global climate model with an atmosphere, ocean, sea-ice and land components through the coupler OASIS. This is comprised of component configurations Global Atmosphere 6.0 (GA6.0), Global Land 6.0 (GL6.0), Global Ocean 5.0 (GO5.0) and Global Sea Ice 6.0 (GSI6.0). The compositions have improved sea-ice parameters over the previous model. The model resolution is N216L85 (~60 km in mid-latitudes) in the atmosphere and ORCA0.25L75 ($0.25^{\circ}$ on a tri-polar grid) in the ocean. In this research, the predictability of each system is evaluated using by RMSE, Correlation and MSSS, and the variables are 500 hPa geopotential height (h500), 850 hPa temperature (t850) and Sea surface temperature (SST). A predictive performance shows that GloSea5GC2 is better than GloSea5GA3. For example, the RMSE of h500 of 1-month forecast is decreased from 23.89 gpm to 22.21 gpm in East Asia. For Nino3.4 area of SST, the improvements to GloSeaGC2 result in a decrease in RMSE, which become apparent over time. It can be concluded that GloSea5GC2 has a great performance for seasonal prediction.

• 대기
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• 제29권2호
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• pp.165-181
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• 2019

In this study, the regional climate model, RegCM4.0 (25 km), with the HadGEM2-AO data as boundary conditions, was used to simulate the mean climate changes in the mid and late 21st century for CORDEX Phase 2 East Asian region. 122 years (1979~2100) of simulation were performed, and RCP 4.5 and RCP 8.5 were used for the simulation of future climate. In the mid-21st century, the temperature is expected to increase by about 0.5 to $3.0^{\circ}C$ in all regions of East Asia, regardless of season and scenario. The increase in temperature is greater in summer and winter, especially in the northern part of simulation domain. Interannual variability (IAV) is expected to decrease by 25% in summer for RCP 8.5, while it is expected to increase by more than 30% in autumn for both scenarios. Regardless of the scenario, the precipitation in South Korea is expected to increase in late June but decrease in mid-July, with an increase in precipitation greater than $100mm\;day^{-1}$. In RCP 4.5 of the late 21st century, relatively uniform temperature increase ($1.0{\sim}2.5^{\circ}C$) is expected throughout the continent, while RCP 8.5 shows a very diverse increase ($3.0{\sim}6.0^{\circ}C$) depending on season and geographical location. In addition, the IAV of temperature is expected to decrease by more than 35% in both scenarios in the summer. In most of the Northwest Pacific region, precipitation is expected to decrease in all seasons except for the summer, but in South Korea, it is projected to increase by about 10% in all seasons except autumn.

• International Journal of Advanced Culture Technology
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• 제7권3호
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• pp.111-115
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• 2019

A 5G is 20 times faster than 4G. It also has hyper-connectivity, low latency merit and boundless potentials for medical education, transportation, entertainment and so on. In accordance with this, it is time to quickly look over on the utilization plan for 5G and sensible media in dance field, deal with the issue and its utilization. First of all, this study will review potential of 5G and sensible media in dance and its development plan. It seems like dance is able to communicate in a three-dimensional way. Utilizing sensible media can contribute to inform people of dance, and increase fun and interest which will make three-dimensional mutual communication. Also, in 5G environment, one can select whatever one wants in his or her viewpoint when utilizing sensible media such as VR, AR, hologram and so on. Supposing in a case of dancers and judges, it is possible for them to hire their own style of dancers in their countries. So, both the dancer and the judges have the positive merits. Third, streaming is possible without any installation, buffering is reduced. At the same time high-definition of media is allowed. This allowed collaborated performance of celebrities in dance and it also increased concentration and engagement. Dance field should acknowledge 5G sensible media, look for systemic and detailed method and disseminate and spread professional training and performance. In dance, testing fast developing sensible media due to 5G network, produce systemic dance training environment with various try is required and an effort for the performance situations in which advanced 5G sensible media is used.

• Bulletin of the Korean Chemical Society
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• 제31권4호
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• pp.934-940
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• 2010

The reduction of [$Os_3(CO)_{11}P(OMe)_3$] and subsequent ionic coupling of the reduced species with $[Ru({\eta}^5-C_5H_5)(CH_3CN)_3]^+$ resulted in the formation of [$Os_3(CO)_{11}P(OMe)_3(Ru({\eta}^5-C_5H_5))_2$] which can be converted to spiked tetrahedral cluster, [$HOs_3(CO)_{11}P(OMe)_3Ru_2({\eta}^5-C_5H_5)(C_5H_4)$] via the intramolecular hydrogen transfer. Due to the unavailability of a suitable single crystal, the PW91/SDD and LDA/SDD density functional methods were used to predict possible structures and the available spectroscopic information (IR, NMR) of [$Os_3(CO)_{11}P(OMe)_3(Ru({\eta}^5-C_5H_5))_2$]. The most probable geometry found by constrained search is the isomer (a2) in which the phosphite, $P(OMe)_3$, occupies an axial position on one of the two osmium atoms that is edge bridged by the $Ru(CO)_2({\eta}^5-C_5H_5)$ unit. By using the most probably geometry, the predicted infrared frequencies and $^1H$, $^{13}C$ and $^{31}P$ NMR chemical shifts of the compound are in the same range as the experimental values. For this type of complex, the LDA/SDD method is appropriate for IR predictions whereas the OPBE/IGLO-II method is appropriate for NMR predictions. The activation energy and reaction energy of the intramolecular hydrogen transfer coupled with the structural change of the transition metal framework were estimated at the PW91/SDD level to be 110.32 and -0.14 kcal/mol respectively.

• 한국콘텐츠학회논문지
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• 제20권12호
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• pp.250-265
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• 2020

N-Screen 환경에서 HTML5 표준 기반 콘텐츠 개발은 필연적이다. 그러나 높은 개발 비용과 개발 인프라 부족 등의 이유로 HTML5 조작형 콘텐츠 개발은 소극적이다. 이에 동영상, 오디오와 같은 멀티미디어 콘텐츠를 동적 조작 구현이 가능한 HTML5와 융합하여 사용자와 상호작용이 가능한 효율적인 콘텐츠 개발 모형을 제안하고자 한다. 제안 모형은 통합 제어용 플레이어를 포함한 HTML5 레이아웃 내에 멀티미디어와 iframe(inline frame) 영역을 구분 배치하도록 설계하였다. 상호작용형 HTML5 문서들은 화면 단위로 나누어 작성하여 iframe을 통하여 제공된다. HTML5의

• 신재생에너지
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• 제7권2호
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• pp.59-69
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• 2011

5MW wind turbine is regarded as a promising system for offshore wind farms in the western sea of Korean. And the wind turbine is developed in many companies but not much information is known about it. In this study, aerodynamics and control characteristics depending on several control methods is reviewed on 5MW wind turbine, in which configuration data of the turbine are used from the previous study of NREL. For the calculations, GH_Bladed, which is certificated software by GL, is used and compared with data from FAST code of NREL. This study shows that how much power production, and aerodynamic performances and loads can be obtained with different controls in the operation of 5MW wind turbine, which is expected to be useful in the design of the wind turbine system.

• Journal of Power Electronics
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• 제13권5호
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• pp.757-765
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• 2013

For conventional interleaved two-phase forward converters with a common output inductor, the maximum duty cycle is 0.5, which limits the voltage range and increases the difficulty of the transformer's optimization. A new two-phase hybrid forward converter with series-parallel auto-regulated transformer windings is presented in this paper. With interleaved control signals for the two phases, the secondary windings of the transformers can work in series when the duty cycle is larger than 0.5, and they can work in parallel when duty cycle is lower than 0.5. Therefore, the maximum duty cycle is extended and the turns ratio of the transformer can be optimized. Duty cycle dependent auto-regulated windings result in the steady states of the converter being different in different duty cycle ranges (D>0.5 and D<0.5). Fortunately, the steady state gains of the proposed hybrid converter are identical at different duty cycle ranges, which means a stepless shift between two states. A prototype is built to verify the theoretical analysis. A conventional control loop is compatible for the whole input voltage range and load range thanks to the stepless shifting between the different duty cycle ranges.