• Biotechnology and Bioprocess Engineering:BBE
• /
• 제10권6호
• /
• pp.482-493
• /
• 2005

Biomass is originally photosynthesized from inorgainic compounds such as $CO_2$, minerals, water and solar energy. Recent studies have shown that anaerobic bacteria have the ability to convert recalcitrant biomass such as cellullosic or chitinoic materials to useful compounds. The biomass containing agricultural waste, unutilized wood and other garbage is expected to utilize as feed, food and fuel by microbial degradation and other metabolic functions. In this study we isolated several anaerobic, cellulolytic and chitinolytic bacteria from rumen fluid, compost and soil to study their related enzymes and genes. The anaerobic and cellulolytic bacteria, Clostridium thermocellum, Clostridium stercorarium, and Clostridium josui, were isolated from compost and the chitinolytic Clostridium paraputrificum from beach soil and Ruminococcus albus was isolated from cow rumen. After isolation, novel cellulase and xylanase genes from these anaerobes were cloned and expressed in Escherichia coli. The properties of the cloned enzymes showed that some of them were the components of the enzyme (cellulase) complex, i.e., cellulosome, which is known to form complexes by binding cohesin domains on the cellulase integrating protein (Cip: or core protein) and dockerin domains on the enzymes. Several dockerin and cohesin polypeptides were independently produced by E. coli and their binding properties were specified with BIAcore by measuring surface plasmon resonance. Three pairs of cohesin-dockerin with differing binding specificities were selected. Two of their genes encoding their respective cohesin polypeptides were combined to one gene and expressed in E. coli as a chimeric core protein, on which two dockerin-dehydrogenase chimeras, the dockerin-formaldehyde dehydrogenase and the dockerin-NADH dehydrogenase are planning to bind for catalyzing $CO_2$ reduction to formic acid by feeding NADH. This reaction may represent a novel strategy for the reduction of the green house gases. Enzymes from the anaerobes were also expressed in tobacco and rice plants. The activity of a xylanase from C. stercorarium was detected in leaves, stems, and rice grain under the control of CaMV35S promoter. The digestibility of transgenic rice leaves in goat rumen was slightly accelerated. C. paraputrificum was found to solubilize shrimp shells and chitin to generate hydrogen gas. Hydrogen productivity (1.7 mol $H_2/mol$ glucos) of the organism was improved up to 1.8 times by additional expression of the own hydrogenase gene in C. paraputrficum using a modified vector of Clostridiu, perfringens. The hydrygen producing microflora from soil, garbage and dried pelletted garbage, known as refuse derived fuel(RDF), were also found to be effective in converting biomass waste to hydrogen gas.

• 디지털융복합연구
• /
• 제15권7호
• /
• pp.259-269
• /
• 2017

본 연구는 의료급여 사례관리의 중재방법별 접근횟수에 따른 효과를 분석함으로써 의료급여 사례관리 중재의 발전을 모색하고자 하였으며, 2010년 3개의 시 군 구에서 고위험군 564명을 대상으로 실시된 사례관리 이차자료 분석연구이다. 중재방법별 접근횟수에 따른 사례관리 전 후 점수는 방문의 경우 자가건강관리능력과 합리적 의료이용에서 유의한 차이를 보였고, 전화는 자가건강관리능력에서, 우편은 건강 삶의 질, 자가건강관리능력, 합리적 의료이용에서 유의한 차이가 있었다. 중재방법별 접근횟수에 따른 의료이용은 방문과 우편이 총급여일수에서 유의한 차이를 보였다. 중재방법별 접근횟수와 사례관리 점수 간의 상관관계에서는 방문이 자가건강관리능력과 음의 관계를, 전화는 합리적 의료이용, 우편은 사례관리 점수 전 영역, 내소는 건강 삶의 질 영역과 양의 관계로 나타났다. 중재방법별 접근횟수와 의료이용 간의 상관관계에서 방문과 자원연계는 총 급여일수와 음의 관계가 있는 것으로 나타났다. 의료급여 과다이용 수급자의 건강 삶의 질 향상과 적정의료이용을 도모하기 위해서는 사례관리 중재방법별 효율적인 접근횟수를 고려하여 사례관리를 실시하는 것이 필요하다.

• 한국균학회소식:학술대회논문집
• /
• 한국균학회 2016년도 춘계학술대회 및 임시총회
• /
• pp.25-25
• /
• 2016

Biological control has many advantages as a disease control method, particularly when compared with pesticides. One of the most important benefits is that biological control is an environmental friendly method and does not introduce pollutants into the environment. Another great advantage of this method is its selectivity. Selectivity is the important factor regarding the balance of agricultural ecosystems because a great damage to non target species can lead to the restriction of natural enemies' populations. The objective of this research was to evaluate the effects of several different bacterial isolates on the efficacy of biological control of soil borne diseases. White rot caused by Sclerotium cepivorum was reported to be severe disease of garlic and chive. The antifungal bacteria Burkholderia pyrrocinia CAB08106-4 was tested in field bioassays for its ability to suppress white rot disease. In field tests, B. pyrrocinia CAB08106-4 isolates suppressed white rot in garlic and chive, with the average control efficacies of 69.6% and 58.9%, respectively. In addition, when a culture filtrate of B. pyrrocinia CAB08106-4 was sprayed onto wounded garlic bulbs after inoculation with a Penicillium hirstum spore suspension in a cold storage room ($-2^{\circ}C$), blue mold disease on garlic bulbs was suppressed, with a control efficacy of 79.2%. These results suggested that B. pyrrocinia CAB08106-4 isolates could be used as effective biological control agents against both soil-borne and post-harvest diseases of Liliaceae. Chinese cabbage clubroot caused by Plasmodiophora brassicae was found to be highly virulent in Chinese cabbage, turnips, and cabbage. In this study, the endophytic bacterium Flavobacterium hercynium EPB-C313, which was isolated from Chinese cabbage tissues, was investigated for its antimicrobial activity by inactivating resting spores and its control effects on clubroot disease using bioassays. The bacterial cells, culture solutions, and culture filtrates of F. hercynium EPB-C313 inactivated the resting spores of P. brassicae, with the control efficacies of 90.4%, 36.8%, and 26.0%, respectively. Complex treatments greatly enhanced the control efficacy by 63.7% in a field of 50% diseased plants by incorporating pellets containing organic matter and F. hercynium EPB-C313 in soil, drenching seedlings with a culture solution of F. hercynium EPB-C313, and drenching soil for 10 days after planting. Soft rot caused by Pectobacterium carotovorum subsp. carotovorum was reported to be severe disease to Chinese cabbage in spring seasons. The antifungal bacterium, Bacillus sp. CAB12243-2 suppresses the soft rot disease on Chinese cabbage with 73.0% control efficacy in greenhouse assay. This isolate will increase the utilization of rhizobacteria species as biocontrol agents against soft rot disease of vegetable crops. Sclerotinia rot caused by Sclerotinia sclerotiorum has been reported on lettuce during winter. An antifungal isolate of Pseudomonas corrugata CAB07024-3 was tested in field bioassays for its ability to suppress scleritinia rot. This antagonistic microorganism showed four-year average effects of 63.1% of the control in the same field. Furthermore, P. corrugata CAB07024-3 has a wide antifungal spectrum against plant pathogens, including Sclerotinia sclerotiorum, Sclerotium cepivorum, Botrytis cinerea, Colletotrichum gloeosporioides, Phytophotra capsici, and Pythium myriotylum.

• 한국IT서비스학회지
• /
• 제17권2호
• /
• pp.17-33
• /
• 2018

The purpose of this study is to verify the moderating effect of mobile divide on the relationship between individual characteristics and the use outcomes of smart device. Data was collected through a survey for periods from May 12, 2017, to May 17, 2017. As a result of multiple regression analysis based on collected data, self-efficacy, information seeking desire and culture capital have a positive effect on the use outcomes of smart device. To examine the moderating effect of mobile divide, we conducted a hierarchical regression analysis by calculating the interaction variables of the independent variable and the mobile literacy. As a result of the analysis, it was found that mobile literacy has negative moderating effect between self-efficacy and the use outcomes of smart device. The results of this study can be interpreted as the higher the self-efficacy and information desire for ICT technology users are. In addition, it can be interpreted that the smart device is convenient in the cultural activities of individuals, and therefore, it is highly utilized. Next, among those who have excellent mobile use ability, those who have high confidence in ICT technology can be interpreted as having low utilization because they recognize that the benefits provided by smart devices are familiar and natural. The results of this study confirm that the digital divide problem in the smart environment is influenced by individual characteristics as well as demographic variables. Therefore, we expect the government to set appropriate policies considering the characteristics of individuals.

• 컴퓨터교육학회논문지
• /
• 제16권4호
• /
• pp.13-21
• /
• 2013

최근 초등학생을 대상으로 다양한 교육용 프로그래밍 언어와 교수학습방법이 개발됨에 따라 교육현장에서 활발하게 프로그래밍 교육이 이루어지고 있다. 그러나 대부분의 프로그래밍 교육이 초등학교 고학년 학생을 대상으로 하고 있으며, 문제해결과정 보다 도구의 사용법에 중점을 두고 있어서 정보교육 전반에 활용되기에는 한계가 있다. 정보교육에서 프로그래밍 활동이 활발히 이루어지기 위해서는 학습대상을 넓히고 교육내용을 사고력 측면에서 고려할 필요성이 있다. 따라서 본 연구에서는 초등학교 저학년 학생도 쉽게 사용할 수 있는 프로그래밍 도구와 사고력 향상을 중심으로 하는 프로그래밍 과제를 제안하였다. 프로그래밍 도구는 텐지블 유저 인터페이스(Tangible User Interface, TUI)를 기반으로 초등학교 저학년 학생도 쉽게 사용할 수 있도록 하였으며, 프로그래밍 과제는 로봇을 활용하여 논리적 사고를 향상시키는 목적으로 개발하였다. 실험을 통해 텐지블 프로그래밍 도구가 초등학교 저학년도 쉽게 사용할 수 있는 도구적 사용성을 나타내었고, 개발한 과제 또한 논리사고력 향상에 효과가 있음을 보여주었다.

• Advances in Traditional Medicine
• /
• 제5권1호
• /
• pp.1-15
• /
• 2005

Ginseng is a popular medicinal plant highly valued throughout the world. Asian ginseng is one of the most common species of ginseng. It has long occupied a significant position in oriental medicine and has been justified its name as the 'king herb'. As a nutritional supplement, ginseng is an extremely common and popular herbal medicine in the United States and Canada in recent decades. The multiple constituents of ginseng possess equally multifaceted pharmacological actions as demonstrated by numerous studies. Ginseng root and its constituents influenced the central nervous system, endocrine, cardiovascular, gastrointestinal system, sexual, renal organ and immune system, etc. One important action is its anti-hyperglycemic effect. Previous studies on ginseng demonstrate that only the root of ginseng has been used in the treatment of diabetes, while the other parts of ginseng plant were always neglected. Recently, we analyzed the constituents of ginseng berry, leaf and discovered that ginseng berry, leaf extracts and its total ginsenosides have the ability to reduce hyperglycemia and body weight and increase the peripheral glucose utilization in obese or diabetic ob/ob or db/db mice. Our data suggest that all parts of ginseng plant, including root, berry, leaf and stem exhibit potent anti-hyperglycemic and anti-obese effects and may provide an opportunity to develop a novel class of anti-diabetic agents.

• KSBB Journal
• /
• 제31권2호
• /
• pp.120-125
• /
• 2016

Two-dimensional cultivation is typically used for cell growth, but the method reduces the characteristics of chondrocytes and stem cells, and limits culture area. Therefore, development of three-dimensional culture method is needed to mimic in vivo environment, improve quality of cells and scale-up efficiently. Improving proliferation and chondrogenesis is available by co-culture of chondrocytes and mesenchymal stem cells (MSCs) that leads to interaction between two kinds of cells. However, the co-culture has problems that permeability of sphere diminishes as aggregate size increased and ratio of two kinds of cells composing each spheres is different. In this work, co-cultivation method using controlled sphere composed of chondrocytes and MSCs was established and enhanced chondrogenesis. Periosteum-derived progenitor cells (PDPCs) that are appropriate for cell therapy source of articular cartilage were used as MSCs. Controlled spheres were formed in the hanging-drop plates and shifted for being induced chondrogenesis in 35-mm non-adhesive culture dishes at a rotation rate of 60 rpm. After inducing chondrogenesis, gene expressions related with chondrogenesis were found to be improved and it was apparent that the utilization of controlled spheres promoted chondrogenesis. As a result, available numbers of cells per unit area were increased and chondrogenic differentiation ability was improved compared to typical two-dimensional culture. This approach shows the potential in cartilage regeneration as it can provide sufficient numbers of chondrocytes.

• 정보관리연구
• /
• 제42권1호
• /
• pp.199-219
• /
• 2011

국내 외의 지식관리와 관련한 연구 대부분은 민간기업을 대상으로 이루어졌고, 공공부문 중에서 지방자치단체의 지식관리시스템 효과나 핵심요인에 대한 구체적인 사례연구는 부족한 상황이고, 그 기존 연구도 대부분 지식관리시스템 만족 요인 탐색인데 반해서 본 연구에서는 지식관리시스템의 이용 정도를 높이기 위해서는 어떤 영향 요인이 중요한지를 탐색하는 데 있다. 본 연구에서는 지식관리시스템이 갖고 있는 어떤 요인이 지식관리시스템 이용 유무에 원인이 되는지 고찰하기 위하여 분석모형에 포함된 지식관리시스템 케퍼빌리티의 구성요소와 개인 및 조직 케퍼빌리티의 구성요소 및 인구 통계학적 요인들을 독립변수로 하여 로짓회귀모형으로 분석 검증한다. '지식관리시스템 이용 유무'에 가장 영향을 미치는 변수는 '지식창조', 다음으로 유의한 변수인 '지식인프라', '부서장의 추진 의지' 요인도 유의하게 산출되었다.

• 사회복지연구
• /
• 제40권3호
• /
• pp.149-179
• /
• 2009

본 연구의 목적은 근로빈곤가구의 주거 상향이동에 영향을 미치는 요인이 무엇인지를 파악하는 것이다. 분석결과를 살펴보면, 연령, 교육수준, 근로능력정도, 가구형태, 아동여부, 재산, 수급형태, 지역, 주거위치, 최저시설기준, 주거환경, 대중교통, 교육시설, 영구임대, 국민임대, 전제자금, 월세지원, 대출연체횟수, 부채 요인이 주거상향이동에 영향을 미치는 것으로 나타났다. 분석결과 따른 주요 함의는 다음과 같다. 첫째, 주거지원정책 수립 시 사회복지 측면에서 접근이 이루어져 수급대상의 가구특성 및 개인특성이 충분히 고려되어야 하고, 이를 위해 주거비 지불능력을 제고시킬 수 있는 임대료보조제도가 실시되어야 할 것이다. 둘째, 근로빈곤가구의 주택대출을 위한 금융권의 접근성을 높여야 할 것이다. 마지막으로 정부에서 실시하는 다양한 주거지원정책에 대한 충분한 홍보와 이용률을 높일 수 있는 노력들이 수반 되어야만 근로빈곤가구의 주거빈곤 문제가 해결될 것이다.

• 한국산업융합학회 논문집
• /
• 제22권1호
• /
• pp.61-69
• /
• 2019

All the parts of lotus, including the seed, rhizome, leaf, stalk, petal, anther, pericarp, and fruit receptacle, have been used in traditional medicine system as a health beneficial supplement. However the most usually used from lotus plant is only the root. Therefore in this study, it will be discussed more the utilization of other parts of the lotus, namely the flower of lotus. The petals and stamens of lotus actually are also rich in bioactive components such as flavonoids and alkaloids, are used in the treatment of tissue inflammation, cancer, skin disease, and also for us as antidotes. One of the biotechnological process that can be used to improve the nutritional content, sensory, and also antioxidant activities is fermentation process. The final product desired from the fermentation process in this study is vinegar. The microbial strain powder used is Uinkin fermented powder with three variations of fermentation. The variations given in this study were initial sugar 32%, 24%, and 14% with the same fermentation temperature, $35^{\circ}C$ for 3 months. The results obtained showed that the pH value and sugar content of products during the fermentation process were decreasing during the fermentation process, with total polyphenol content of $283.7{\pm}97.6mg/100g\;QAE$, and total flavonoid content of $3.3{\pm}0.0mg/100g\;QAE$. For the DPPH radical scavenging ability of the fermentation product also increased in a concentration dependent manner, with ORAC activity of the product showed a high activity of $20.7{\pm}0.41{\mu}M$ TE. Therefore, fermentation process can be the one of method for improving the product. The efficiency of lotus flower vinegar fermentation can be reached with an initial sugar condition of 25% (sample B).