• The Journal of Korean Medicine
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• v.31 no.4
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• pp.20-37
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• 2010

Objective: This study was performed to investigate the effect of Yagwan-cheunghyeoltang (YCT) on insulin secretion in RIN-m5F cells. Methods: After treatment with various concentrations of YCT to RIN-m5F cells, cell viability, free radical-scavenging activity, SOD activity, and insulin secretion were measured. Additionally, insulin-related gene expressions were measured using real-time RT-PCR. Results: 1. YCT didn't show any influence on RIN-m5F cells viability. 2. YCT showed free radical-scavenging activity by 16% at $100{\mu}g/m{\ell}$ of concentration. 3. YCT showed enhancement of SOD activity by 60% at $100{\mu}g/m{\ell}$ of concentration. 4. YCT significantly increased insulin secretion in RIN-m5F cells in a dose-dependent manner. 5. YCT up-regulated INS-1, INS-2, IRS-1, IRS-2 and IRS-3 mRNA expressions compared to the control group. 6. YCT down-regulated INS-R, GCK, GLP-1R and GLP-2R mRNA expressions compared to the control group. Conclusion: YCT has pharmaceutical properties enhancing insulin production and controlling glucose-associated metabolism, and could be a candidate for drug development after further research.

• The Korea Journal of Herbology
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• v.31 no.1
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• pp.1-5
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• 2016

Objectives : The purpose of this study was to investigate inhibitory effects of steamed Polygonatum odoratum extract (POE) on insulin resistance in rat skeletal muscle cells, L6 cells.Methods : Polygonatum odoratum (P. odoratum) extract was extracted with ethyl acetate. Activity of α-glucosidase in POE was measured for blood glucose regulation. MTT assay was examined for cell toxicity. Western blot analysis for measurement of adiponectine, peroxisome proliferator-activated receptorγ (PPARγ), insulin receptor substrate (IRS), glucose transporter 4 (Glut-4) and phosphorylation of serine/threonine-specific protein kinase (Akt) expressions were performed. Akt signaling pathway were analyzed with LY294002, which is a specific PI3K/Akt inhibitor.Results : The results revealed that POE inhibited α-glucosidase activity. Treatment of POE in L6 cells inhibited the differentiation of L6 cells compared to those of vehicl control. Additionally, protein expressions of adiponectine, PPARγ, IRS and Glut-4 were significantly regulated compared to those of vehicle control (p < 0.05), respectively. Futhermore, phosphorylation of Akt was increased in L6 cells treated with POE compared to that of vehicle control (p < 0.05). pAkt expression was significantly accentuated with Akt inhibitor (LY294002).Conclusions : These results suggest that POE may have potential as a natural agent for prevention/improvement of diabetes, especially, regulation of blood glucose. Therefore, further additional study should be conducted to elucidate in depth the pharmaceutical efficacy of these.

• Journal of the Korea Institute of Military Science and Technology
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• v.20 no.3
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• pp.337-344
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• 2017

In this paper, the optical axis alignment(OAA) of an infrared sensor(IRS) using a microwave sensor(MWS) was presented as a method of an axis alignment to minimize the problems that could be caused by the misalignment of the two sensors in the missile including the dual-mode sensor. The azimuth(AZ) and elevation(EL) angles of the two targets used for each sensor test were calculated by using the transformation equation and the test results of the MWS and IRS, and then the proposed OAA was verified by comparing the angles. Furthermore, the validity of the proposed OAA was demonstrated by confirming the abnormality of the OAA through the test results of the electro optical head(EOH) of the IRS which was equipped with a tilt on the missile fuselage.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.31 no.2 s.257
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• pp.211-220
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• 2007

This work presents an iterated improved reduced system (IIRS) procedure combined with sub-structuring scheme for large structures. Iterated IRS methods are usually more efficient than others because the dynamic condensation matrix is updated repeatedly until the desired convergent values are obtained. However, using these methods simply for large structures causes expensive computational cost and even makes analyses intractable because of the limited computer storage. Therefore, the application of sub-structuring scheme is necessary. Because the large structures are subdivided into several (or more) sub-domains, the construction of dynamic condensation matrix does not require much computation cost in every iteration. This makes the present method much more efficient to compute the eigenpairs both in lower and intermediate modes. In Part I, iterated IRS method combined with sub-structuring scheme for undamped structures is presented. The validation of the proposed method and the evaluation of computational efficiency are demonstrated through the numerical examples.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.31 no.2 s.257
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• pp.221-230
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• 2007

An iterated improved reduced system (IIRS) procedure combined with sub-structuring scheme for nonclassically damped structural systems is presented. For dynamic analysis of such systems, complex eigenproperties are required to incorporate properly the nonclassical damping effect. In complex structural systems, the equations of motion are written in the state space from. Thus, the number of degrees of freedom of the new equations of motion and the size of the associated eigenvalue problem required to obtain the complex eigenvalues and eigenvectors are doubled. Iterated IRS method is an efficient reduction technique because the eigenproperties obtained in each iteration step improve the condensation matrix in the next iteration step. However, although this reduction technique reduces the size of problem drastically, it is not efficient to apply this technique to a single domain finite element model with degrees of freedom over several thousands. Therefore, for a practical application of the reduction method, accompanying sub-structuring scheme is necessary. In the present study, iterated IRS method combined with sub-structuring scheme for nonclssically damped structures is developed. Numerical examples demonstrate the convergence and the efficiency of a newly developed scheme.

• Proceedings of the Botanical Society of Korea Conference
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• 1987.07a
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• pp.391-401
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• 1987

Plant chloroplast DNA exists as an unique circular structure in which large single copy(LSC) region and small single copy (SSC) region are separated by large inverted repeat sequences (IRS). It has been known that the unique existence of inverted repeat sequences in chloroplast DNA has no relation with the stability of the chloroplast DNA, but causes the inversion between inverted repeat its biological significance has not been understood so far. In rice, several gene clusters have been cloned and sequenced which contain ribulose-5-biophosphate car-boxylase large subunit (rbcL). Especially, one rbcL gene is linked with rp12 gene which is located in the IRS region in one of the gene clusters. By comparison of nucleotide sequence, the two genes are found to be linked through 151 bp repeat sequence which is homologous to the rp123 gene in IRS region. The repeat sequence is found to be located 3' downstream of rfcL gene and near psbA gene in LSC region. The existence of these repeat sequences and the presence of gene clusters caused by the gene rearrangement thorough the repeat sequence provide a possible which is found to be dispersed chloroplast DNA provide the model system to explaine the heterogeneity of the chloroplast DNA in rice in term of gene rearrangement.

• Proceedings of the Korea Information Processing Society Conference
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• 2014.11a
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• pp.639-641
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• 2014

효율적인 인터페이스 정의 및 관리를 위하여 개발된 MDMS (Message Definition and Management System) 는 인터페이스 요구사항명세서(IRS: Interface Requirement Specification)과 인터페이스 설계기술서 (IDD: Interface Design Description), 인터페이스 구현 및 통합의 단계로 이루어진 인터페이스 개발 과정을 통합 관리할 수 있도록 해준다. 이러한 통합관리는 인터페이스 개발에 따른 IRS, IDD, 구현코드에 걸친 일관성 유지를 쉽게 해주며, 소프트웨어 개발 과정에서 가장 문제가 많이 발생하는 단계라고 할 수 있는 소프트웨어 통합에 있어서 인터페이스 불일치로 인한 문제의 대부분을 제거해 준다. MDMS 기반의 인터페이스 관리는 한번의 인터페이스 정의로 IRS, IDD, 송수신 코드까지 생성함으로써 인터페이스 정의 및 개발의 단계를 한단계로 축소하여 각 단계 사이의 불일치 발생이 미연에 방지되며, 일관성 유지 노력이 필요없다. 삼성탈레스에서는 2008 년 이후 10 여개의 함정전투체계 프로젝트의 소프트웨어 개발에 MDMS 를 적용하여 소프트웨어 개발 생산성의 향상을 이루었다. 특히 인터페이스 정의, 설계, 문서 작성, 코드 구현, 형상관리가 통합은 소프트웨어 인터페이스 개발과정에서의 불필요한 중복 작업을 최소화하고, 그에 따른 문서와 코드간의 불일치를 근본적으로 제거할 수 있었다. 또한, 소프트웨어 개발 관리 측면에서도 인터페이스의 복잡도를 평가하여, 향후 유사 시스템의 소프트웨어 아키텍처 구성에 활용할 수 있다.

• Journal of Physiology & Pathology in Korean Medicine
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• v.34 no.3
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• pp.136-141
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• 2020

The purpose of this study was to investigate the action mechanism of the roots of Adenophora triphylla var. japonica extract (ATE) in 3T3-L1 adipocytes. Cell toxicity test by MTT assay and lipid accumulation was performed to evaluate the inhibitory effect on the differentiation of adipocyte from preadipocytes induced by MDI differentiation medium, while adipogenesis related proteins expression level were evaluated by western blotting. As a result, ATE inhibited MDI-induced adipocyte differentiation in 3T3-L1 cells dose-dependently without cytotoxicity. Our results showed that ATE inhibited the phosphorylation of IRS1, thereby decreasing the expression of PI3K110α and reducing the phosphorylation of AKT and mTOR, resulting in attenuated protein expression of C/EBPα, PPARγ, ap2 and FAS in 3T3-L1 cells. These results suggest anti-adipogenic functions for ATE, and identified IRS1 as a novel target for ATE in adipogenesis.

• Journal of the Korean Association of Geographic Information Studies
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• v.5 no.4
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• pp.86-92
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• 2002

The feasibility of counting number of small vessels and position in Korean waters using the panchromatic imageries derived from the IRS-1C was tested. The parameters for interpretation of satellite's imageries of small vessels were location(position), size, shape, shadow, tone, texture and pattern, height and depth, situation and association with other vessels. The position of small vessels in the sea without GCP(ground control point) was considered to be inclusive in the satellite imagery with 35 km semi-diameter, denoting rough geographical position of the vessel. The size of vessel was measured by length from stem to stern of the vessel, distinguished by following wave on the surface water. Offshore fishing vessels were separated from merchant ships by their length smaller than 100 m. The shape of vessels on panchromatic imagery of IRS-1C appeared just streamline. In case of clouds which were similar to the shape of small vessels, we were able to distinguish between vessel and cloud by shadow of cloud in the water surface. The tone of sea surface was dark black while small vessel appeared bright white. Small vessel was distinguished from the rough texture of the sea surface and the regular pattern of the waves with white capes when weather was not so good. The situation of the fishing activity was estimated by information of fishing method related to the fishing boat such as the pair trawl in the Yellow Sea.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.10
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• pp.5675-5680
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• 2013

Background: Preexisting type 2 diabetes mellitus (T2DM) affects the prognosis and mortality of patients with some cancers. Insulin like growth factor (IGF) and insulin receptor (IR) signaling axes play important roles in both cancer and diabetes development. We aimed to explore the expression characteristics of proteins in IGF/IR axis in non-small cell lung cancer (NSCLC) cases with preexisting T2DM. Methods: Fifty-five NSCLC patients with preexisting T2DM were retrospectively included and matched by 55 NSCLC without diabetes at a 1:1 ratio. The expression of proteins in IGF/IR axis was detected by immunohistochemical staining. Clinicopathological data were collected to analyze their relationship with the protein expression. Results: Both IGF 1 receptor (IGF-1R) and insulin receptor substrate 2 (IRS-2) showed higher expression in the NSCLC with T2DM group, compared with those without T2DM. The high expression of IGF-1R and IRS-2 were found to be negatively associated with lymph node metastases and T staging in the T2DM group, respectively, and IRS-2 expression was also found more in the subgroup whose T2DM duration was more than 4 years. No difference was detected in the expression of IRS-1, IGF-1, IGF-2, IGFBP3, IR and mTOR between groups with or without T2DM. Conclusion: Our study found higher expression of IGF-1R and IRS-2 proteins in NSCLC patients with preexisting T2DM, and that there was an association with early stage NSCLC, which suggested that IGF signaling may play an important early event in development of NSCLC associated with diabetes.