• BMB Reports
• /
• 제45권6호
• /
• pp.360-364
• /
• 2012

Uptake of circulating glucose into the cells happens via the insulin-mediated signalling pathway, which translocates the glucose transporter 4 (GLUT4) vesicles from the intracellular compartment to the plasma membrane. Rab GTPases are involved in this vesicle trafficking, where Rab GTPases-activating proteins (RabGAP) enhance the GTP to GDP hydrolysis. TBC1D4 (AS160) and TBC1D1 are functional RabGAPs in the adipocytes and the skeletonal myocytes, respectively. These proteins contain two phosphotyrosine-binding domains (PTBs) at the amino-terminus of the catalytic RabGAP domain. The second PTB has been shown to interact with the cytoplasmic region of the insulin-regulated aminopeptidase (IRAP) of the GLUT4 vesicle. In this study, we quantitatively measured the ${\sim}{\mu}M$ affinity ($K_D$) between TBC1D4 PTB and IRAP using isothermal titration calorimetry, and further showed that IRAP residues 1-49 are the major region mediating this interaction. We also demonstrated that the IRAP residues 1-15 are necessary but not sufficient for the PTB interaction.

• 천문학논총
• /
• 제27권4호
• /
• pp.195-200
• /
• 2012

We show how the rotation emission from isolated interstellar Polycyclic Aromatic Hydrocarbons (PAHs) can explain the so-called anomalous microwave emission (AME). AME has been discovered in the last decade as microwave interstellar emission (10 to 70 GHz) that is in excess compared to the classical emission processes: thermal dust, free-free and synchrotron. The PAHs are the interstellar planar nano-carbons responsible for the near infrared emission bands in the 3 to 15 micron range. Theoretical studies show that under the physical conditions of the interstellar medium (radiation and density) the PAHs adopt supra-thermal rotation velocities, and consequently they are responsible for emission in the microwave range. The first results from the PLANCK mission unexpectedly showed that the AME is not only emitted by specific galactic interstellar clouds, but it is present throughout the galactic plane, and is particularly strong in the cold molecular gas. The comparison of theory and observations shows that the measured emission is fully consistent with rotation emission from interstellar PAHs. We draw the main lines of our PLANCK-AKARI collaborative program which intends to progress on this question by direct comparison of the near infrared (AKARI) and microwave (PLANCK) emissions of the galactic plane.

• Journal of Astronomy and Space Sciences
• /
• 제17권2호
• /
• pp.181-188
• /
• 2000

DO Cas는 최근 Shaw (1990)에 의해 근접촉쌍성으로 분류된 공전주기 0.68일의 식쌍성이다. 짧은 공전주기를 가짐에도 불구하고 Mannino (1958) 이후 분광관측에 의한 시선속도 곡선이 발표되지 않고 있다. 그런데 Hill (1991)은 Mannino의 시선속도곡선이 오류를 포함하고 있는 것으 로 보인다고 하였다. 이 연구에서 우리는 Asiago 천문대에 의뢰하여 Mannino가 분석한 분광관측 사진건판을 입수하여 PDS로 측정하여 IRAP로 재분석하였다. 이 시선속도곡선과 1998년에 관측한 BVR 광도곡선을 Wilson-Devinney 방법으로 동시에 분석하였다. 그 결과 DO Cas가 접촉 또는 주성이 로쉬엽을 채우고 있는 접촉에 매우 가까운 준분리형 쌍성임을 확인하였으며 또한 이 계의 절대량을 구하였다.

• Parasites, Hosts and Diseases
• /
• 제41권4호
• /
• pp.209-219
• /
• 2003

The evolutionary course of the CsRn1 long-terminal-repeat (LTR) retrotransposon was predicted by conducting a phylogenetic analysis with its paralog LTR sequences. Based on the clustering patterns in the phylogenetic tree, multiple CsRn1 copies could be grouped into four subsets, which were shown to have different integration times. Their differential sequence divergences and heterogeneous integration patterns strongly suggested that these subsets appeared sequentially in the genome of C. sinensis. Members of recently expanding subset showed the lowest level of divergence in their L TR and reverse transcriptase gene sequences. They were also shown to be highly polymorphic among individual genomes of the trematode. The CsRn1 element exhibited a preference for repetitive, agenic chromosomal regions in terms of selecting integration targets. Our results suggested that CsRn1 might induce a considerable degree of intergenomic variation and, thereby, have influenced the evolution of the C. sinensis genome.

• 천문학논총
• /
• 제32권1호
• /
• pp.97-99
• /
• 2017

Our understanding of dust emission, interaction, and evolution, is evolving. In recent years, electric dipole emission by spinning dust has been suggested to explain the anomalous microwave excess (AME), appearing between 10 and 90 Ghz. The observed frequencies suggest that spinning grains should be on the order of 10nm in size, hinting at polycyclic aromatic hydrocarbon molecules (PAHs). We present data from the AKARI/Infrared Camera (IRC) due to its high sensitivity to the PAH bands. By inspecting the IRC data for a few AME regions, we find a preliminary indication that regions well-fitted by a spinning-dust model have a higher $9{\mu}m$ than $18{\mu}m$ intensity vs. non-spinning-dust regions. Ongoing efforts to improve the analysis by using DustEM and including data from the AKARI Far Infrared Surveyor (FIS), IRAS, and Planck High Frequency Instrument (HFI) are described.

• 천문학회보
• /
• 제42권2호
• /
• pp.66.2-66.2
• /
• 2017

The Korea Astronomy and Space Science Institute plans to develop a coronagraph in collaboration with National Aeronautics and Space Administrative (NASA) and install it on the International Space Station (ISS). The coronagraph is an externally occulted one stage coronagraph with a field of view from 2.5 to 15 solar radii. The observation wavelength is approximately 400 nm where strong Fraunhofer absorption lines from the photosphere are scattered by coronal electrons. Photometric filter observation around this band enables the estimation of 2D electron temperature and electron velocity distribution in the corona. Together with the high time cadence (< 12 min) of corona images to determine the geometric and kinematic parameters of coronal mass ejections, the coronagraph will yield the spatial distribution of electron density by measuring the polarized brightness. For the purpose of technical demonstration, we intend to observe the total solar eclipse in 2017 August for the filter system and to perform a stratospheric balloon experiment in 2019 for the engineering model of the coronagraph. The coronagraph is planned to be installed on the ISS in 2021 for addressing a number of questions (e.g. coronal heating and solar wind acceleration) that are both fundamental and practically important in the physics of the solar corona and of the heliosphere.

• 천문학회지
• /
• 제50권5호
• /
• pp.139-149
• /
• 2017

The Korea Astronomy and Space Science Institute plans to develop a coronagraph in collaboration with National Aeronautics and Space Administration (NASA) and to install it on the International Space Station (ISS). The coronagraph is an externally occulted one-stage coronagraph with a field of view from 3 to 15 solar radii. The observation wavelength is approximately 400 nm, where strong Fraunhofer absorption lines from the photosphere experience thermal broadening and Doppler shift through scattering by coronal electrons. Photometric filter observations around this band enable the estimation of 2D electron temperature and electron velocity distribution in the corona. Together with a high time cadence (<12 min) of corona images used to determine the geometric and kinematic parameters of coronal mass ejections, the coronagraph will yield the spatial distribution of electron density by measuring the polarized brightness. For the purpose of technical demonstration, we intend to observe the total solar eclipse in August 2017 with the filter system and to perform a stratospheric balloon experiment in 2019 with the engineering model of the coronagraph. The coronagraph is planned to be installed on the ISS in 2021 for addressing a number of questions (e.g., coronal heating and solar wind acceleration) that are both fundamental and practically important in the physics of the solar corona and of the heliosphere.

• 천문학논총
• /
• 제30권2호
• /
• pp.439-442
• /
• 2015

We observed multiple CO transition lines and the HCN(1-0) line at ~ 1" (~ 34 pc) or higher resolution toward the Seyfert 2 nucleus of M51 using the IRAM Plateau de Bure Interferometer (PdBI) and the Submillimeter Array (SMA). All the images show very similar overall molecular gas distribution; there are two discrete clouds at the eastern and western sides of the nucleus, and the western cloud exhibits an elongated distribution and velocity gradient along the radio jet. In addition, high HCN(1-0)/CO(1-0) brightness temperature ratios of about unity have been observed, especially along the radio jet, similar to those observed in shocked molecular gas in our Galaxy. This strongly indicates that the molecular gas along the jet is shocked, that the radio jet and the molecular gas are interacting, and the jet is entraining both diffuse (CO) and dense (HCN) molecular gas outwards from the circumnuclear region. This is the first clear imaging of the outflowing molecular gas entrained by the AGN jet, and showing the detailed physical status of outflowing molecular gas. Since a relatively high HCN(1-0)/CO(1-0) ratio has been observed in the high velocity wing of ultraluminous infrared galaxies, it can also be explained by a similar mechanism to those we describe here.

• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 2002년도 창립10주년기념 및 국립독성연구원 의약품동등성평가부서 신설기념 국재학술대회:생물학적 동등성과 의약품 개발 전략을 위한 국제심포지움
• /
• pp.113-113
• /
• 2002

Phylogenetically conserved Bcl-2 family proteins play a pivotal role in the regulation of apoptosis from virus to human. Members of the Bcl-2 family consist of antiapoptotic proteins such as Bcl-2, Bcl-xL, and Bcl-w, and proapoptotic proteins such as BAD, Bax, BOD, and Bok. It has been proposed that anti- and proapoptotic Bcl-2 proteins regulate cell death by binding to each other and forming heterodimers. A delicate balance between anti- and proapoptotic Bcl-2 family members exists in each cell and the relative concentration of these two groups of proteins determines whether the cell survives or undergoes apoptosis. Mcl-1 (Myeloid cell :leukemia-1) is a member of the Bcl-2 family proteins and was originally cloned as a differentiation-induced early gene that was activated in the human myeloblastic leukemia cell line, ML-1 . Mcl-1 is expressed in a wide variety of tissues and cells including neoplastic ones. We recently identified a short splicing variant of Mcl-1 short (Mcl-IS) and designated the known Mcl-1 as Mcl-1 long (Mcl-lL). Mcl-lL protein exhibits antiapoptotic activity and possesses the BH (Bcl-2 homology) 1, BH2, BH3, and transmembrane (TM) domains found in related Bcl-2 proteins. In contrast, Mcl-1 S is a BH3 domain-only proapoptotic protein that heterodimerizes with Mcl-lL. Although both Mc1-lL and Mcl-lS proteins contain BH domains fecund in other Bcl-2 family proteins, they are distinguished by their unusually long N-terminal sequences containing PEST (proline, glutamic acid, serine, and threonine) motifs, four pairs of arginine residues, and alanine- and glycine-rich regions. In addition, the expression pattern of Mcl-1 protein is different from that of Bcl-2 suggesting a unique role (or Mcl-1 in apoptosis regulation. Tankyrasel (TRF1-interacting, ankyrin-related ADP-related polymerasel) was originally isolated based on its binding to TRF 1 (telomeric repeat binding factor-1) and contains the sterile alpha motif (SAM) module, 24 ankyrin (ANK) repeats, and the catalytic domain of poly(adenosine diphosphate-ribose) polymerase (PARP). Previous studies showed that tankyrasel promotes telomere elongation in human cells presumably by inhibiting TRFI though its poly(ADP-ribosyl)action by tankyrasel . In addition, tankyrasel poly(ADP-ribosyl)ates Insulin-responsive amino peptidase (IRAP), a resident protein of GLUT4 vesicles, and insulin stimulates the PARP activity of tankyrase1 through its phosphorylation by mitogen-activated protein kinase (MAPK). ADP-ribosylation is a posttranslational modification that usually results in a loss of protein activity presumably by enhancing protein turnover. However, little information is available regarding the physiological function(s) of tankyrase1 other than as a PARP enzyme. In the present study, we found tankyrasel as a specific-binding protein of Mcl-1 Overexpression of tankyrasel led to the inhibition of both the apoptotic activity of Mel-lS and the survival action of Mcl-lL in mammalian cells. Unlike other known tankyrasel-interacting proteins, tankyrasel did not poly(ADP-ribosyl)ate either of the Mcl-1 proteins despite its ability to decrease Mcl-1 proteins expression following coexpression. Therefore, this study provides a novel mechanism to regulate Mcl-1-modulated apoptosis in which tankyrasel downregulates the expression of Mcl-1 proteins without the involvement of its ADP-ribosylation activity.

• 천문학논총
• /
• 제30권2호
• /
• pp.79-82
• /
• 2015

The physical and chemical properties of prestellar cores, especially massive ones, are still far from being well understood due to the lack of a large sample. The low dust temperature (< 14 K) of Planck cold clumps makes them promising candidates for prestellar objects or for sources at the very initial stages of protostellar collapse. We have been conducting a series of observations toward Planck cold clumps (PCCs) with ground-based radio telescopes. In general, when compared with other star forming samples (e.g. infrared dark clouds), PCCs are more quiescent, suggesting that most of them may be in the earliest phase of star formation. However, some PCCs are associated with protostars and molecular outflows, indicating that not all PCCs are in a prestellar phase. We have identified hundreds of starless dense clumps from a mapping survey with the Purple Mountain Observatory (PMO) 13.7-m telescope. Follow-up observations suggest that these dense clumps are ideal targets to search for prestellar objects.