• 한국산림과학회지
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• 제110권1호
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• pp.116-127
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• 2021

본 연구는 나라꽃인 무궁화의 주요 품종별 해충상을 조사하고, 무궁화 30품종에서 발생하는 주요 해충의 밀도를 비교하기 위해서 수행되었다. 2018년부터 2020년까지 수원과 부산에서 육안조사법으로 해충상을 조사한 결과, 개체수의 대부분을 차지하는 목화진딧물을 제외하고 부산에서는 20종 1,147개체의 해충이 채집되었고, 수원에서는 31종 2,240개체의 해충이 채집되었다. 품종에 따른 종수와 개체수 차이는 없었으나, 지역에 따른 차이는 있었다. 우점종은 목화진딧물, 점노랑들명나방, 왕붉은잎큰나방, 목화명나방, 썩덩나무노린재의 순이었다. 우점종의 발생 역시 품종 보다는 조사 지역과 연도에 따른 차이만 있었다. 결론적으로 무궁화의 품종 차이가 해충상 및 주요 해충의 발생에 미치는 영향은 확인할 수 없었으며, 무궁화가 식재된 주변 환경의 차이가 해충상에 큰 영향을 주는 것으로 판단된다.

• Journal of Applied Biological Chemistry
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• 제43권4호
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• pp.222-229
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• 2000

In this paper we will assess the feasibility of some insect behavior-modifying chemicals for insect control, such as male orientation inhibitor, female calling interrupter and female attractant of cotton bollworm Helicoverpa armigera. These behavior-modifying chemicals have advantages of simple chemical structure, easy to synthesize and low price of products. The effects of sub-lethal insecticides on insect chemical communication system and the differences of pheromone communication systems between the resistant and susceptible strain of H. armigera will also be discussed.

• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2005년도 춘계 학술발표회
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• pp.150-150
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• 2005

• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2005년도 춘계 학술발표회
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• pp.151-151
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• 2005

• International Journal of Industrial Entomology and Biomaterials
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• 제33권2호
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• pp.59-62
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• 2016

Many insects have gained increasing attention as an alternative protein for humans. Among those, the two-spotted cricket, Gryllus bimaculatus (Orthoptera: Gryllidae), was recently approved as a general food ingredient by the Korean Ministry of Food and Drug Safety. For industrial utilization of G. bimaculatus, mass rearing techniques and production system should be standardized first. In this study, we investigated the effects of five different feeds on the growth and development of G. bimaculatus. Feed is the one of the key factors that has considerable effects on rearing insects. With five different kinds of feed on $1^{st}$, $3^{rd}$, and $5^{th}$ instar nymphs, the change of survival rate, body weight were monitored up to eight wk after hatching. We concluded that 50% of soybean flour, 20% of corn powder, 10% of rice bran, 9% of milk serum, 10% of rice flour, 0.5% of microorganisms, and 0.5% of multivitamins and minerals (diet C) was the best mix for promoting growth and development of $3^{rd}$ instar nymphs compared to the control diet.

• 한국응용곤충학회지
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• 제48권4호
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• pp.453-457
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• 2009

본 연구는 2005년부터 2007년까지 수원, 영양, 부여와 예천의 곤충 생태원 부지에서 곤충강의 다양성을 조사하였다. 월별 곤충 개체군 크기는 6월부터 8월에 네 조사구역 모두에서 가장 컸다. 전체적으로 딱정벌레목의 곤충이 가장 많이 채집되었으며, 노린재목과 메뚜기목의 곤충이 뒤를 이었다. 각 곤충생태원은 지리적 특색에 맞는 고유한 교육프로그램을 운영하고 있었다. 이러한 연구와 생태원의 활동은 그 지역의 곤충과 같은 생물의 다양성을 보존하고 관람객에게 인식시키는데 중요한 역할을 할 것이다.

• 대한의생명과학회지
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• 제7권4호
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• pp.161-166
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• 2001

Most mammalian cells take up glucose by passive transport proteins in the plasma membranes. The best known of these proteins is the human erythrocyte glucose transporter, GLUT1. High levels of heterologous expression far the transporter are necessary for the investigation of its three-dimensional structure by crystallization. To achieve this, the baculovirus expression system has become popular choice. However, Spodoptera frugiperda Clone 9 (Sf9) cells, which are commonly employed as the host permissive cell line to support baculovirus replication and protein synthesis, grow well on TC-100 medium that contains 0.1% D-glucose as the major carbon source, suggesting the presence of endogenous glucose transporters. Furthermore, very little is known of the endogenous transporters properties of Sf9 cells. Therefore, human GLUT1 antibodies would play an important role for characterization of the GLUT1 expressed in insect cell. However, the successful use of such antibodies for characterization of GLUT1 expression m insect cells relies upon their specificity for the human protein and lack of cross-reaction with endogenous transporters. It is therefore important to determine the potential cross-reactivity of the antibodies with the endogenous insect cell glucose transporters. In the present study, the potential cross-reactivity of the human GLUT1 antibodies with the endogenous insect cell glucose transporters was examined by Western blotting. Neither the antibodies against intact GLUT1 nor those against the C-terminus labelled any band migrating in the region expected fur a protein of M$_r$ comparable to GLUT1, whereas these antibodies specifically recognized the human GLUT1. Specificity of the human GLUT1 antibodies tested was also shown by cross-reaction with the GLUT1 expressed in insect cells. In addition, the insect cell glucose transporter was found to have very low affinity for cytochalasin B, a potent inhibitor of human erythrocyte glucose transporter.

• International Journal of Industrial Entomology and Biomaterials
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• 제29권2호
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• pp.198-206
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• 2014

The olive fly Bactrocera oleae (Rossi) is the key pest for olive cultivation worldwide. Substantial effort has been invested in the development of the sterile insect technique (SIT) to control this pest. One of the limitations to develop SIT technology for olive fruit fly is the low ability of wild females to lay eggs in other medium than olive fruits, and their slow adaptation to oviposition in artificial substrates. In the present study, fruit grapes were used as an alternative egg collection medium to harvest eggs and young larvae from freshly colonized wild strains originating from France, Italy, Spain and Croatia. The larvae were allowed to develop into the fruits until the second instar, before they were extracted out and further reared on a standard artificial diet. Furthermore, F1 to F4 female flies were alternatively offered wax bottles to oviposit. Finally, the performance of hybrid strains created from crosses between wild and long colonised flies was assessed. The results showed that females of all 4 wild strains readily oviposited eggs in grapes and from the F2 generation onward, females from all strains were adapted to laying eggs in wax bottles. No difference was observed in eggs and pupae production among all strains tested. The findings are discussed for their implications on SIT application against olive fruit fly.

• Journal of Microbiology and Biotechnology
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• 제17권5호
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• pp.761-768
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• 2007

Serratia proteamaculans HY-3 isolated from the digestive tract of a spider produces an extracellular protease named arazyme, with an estimated molecular mass of 51.5 kDa. The purified enzyme was characterized as having high activities at wide pH and temperature ranges. We further characterized biochemical features of the enzymatic reactions under various reaction conditions. The protease efficiently hydrolyzed a broad range of protein substrates including albumin, keratin, and collagen. The dependence of enzymatic activities on the presence of metal ions such as calcium and zinc indicated that the enzyme is a metalloprotease, together with the previous observation that the proteolytic activity of the enzyme was not inhibited by aspartate, cysteine, or serine protease inhibitors, but strongly inhibited by 1,10-phenanthroline and EDTA. The araA gene encoding the exoprotease was isolated as a 5.6 kb BamHI fragment after PCR amplification using degenerate primers and subsequent Southern hybridization. The nucleotide sequence revealed that the deduced amino acid sequences shared extensive similarity with those of the serralysin family of metalloproteases from other enteric bacteria. A gene(inh) encoding a putative protease inhibitor was also identified immediately adjacent to the araA structural gene.

• 한국정보통신학회논문지
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• 제11권8호
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• pp.1604-1611
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• 2007

곤충의 발자국 패턴을 이용하여 곤충을 인식하고자 할 때에는 특징을 추출하기 위한 기본 단위의 영역을 추출할 필요가 있다. 이 논문에서는 기본 단위 영역의 추출을 위한 전 단계 처리 과정으로서 군집화 기법을 사용하였다. 인식의 대상이 되는 곤충들의 크기와 종류에 따라 남겨지는 발자국 패턴의 크기 및 간격이 다르게 나타난다. 따라서 이 논문에서는 패턴의 크기와 간격에 관계없이 인식의 기본 단위가 되는 영역을 추출할 수 있도록 하는 개선된 ART2 알고리즘을 제안하였다. 제안한 ART2 알고리즘에서는 군집화를 위한 임계값이 군집화의 대상이 되는 모든 패턴들의 거리를 축적한 그래프의 형태에 따라 자동으로 설정되도록 하였다. 제안한 기법으로 2 가지 종류의 곤충 발자국 패턴에 대하여 군집화를 실험한 결과 모두 바르게 군집화가 이루어짐을 알 수 있었다.