• Tuberculosis and Respiratory Diseases
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• 제56권6호
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• pp.611-618
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• 2004

서 론 : 특발성 폐섬유화증은 원인 모르게 폐 내에 섬유화가 진행하여 진단 후 중간생존기간이 3-5년 정도로 보고되는 치명적인 질환이나 생존기간을 증가시키는 효과가 있는 치료제는 아직 알려져 있지 않다. 최근 스테로이드나 다른 면역억제제 치료에 반응이 없는 IPF에서 INF-${\gamma}$이 효과가 있다는 보고가 있었으나 아직은 많은 논란이 있어 진행된 IPF 환자에서 INF-${\gamma}$ 효과를 관찰하기 위하여 본 연구를 시행하였다. 방 법 : 1999년 1월부터 2001년 3월까지 IPF-UIP로 진단되었던 환자 중 스테로이드제제 및 기존의 면역억제제에 반응을 보이지 않았던 9명 (연령 $55.4{\pm}15.3$세, 남:여=8:1)을 대상으로 INF-${\gamma}$ 200만 unit를 주당 3회 피하주사로 12개월간 저용량 스테로이드와 합께 투여하였다. INF-${\gamma}$ 투여시에도 UIP가 진행되거나 심각한 부작용 시 투여를 중단하였다. 결 과 : 1) 전체 9례 중 5례는 INF-${\gamma}$ 치료를 도중에 중단하였고 총 4례만이 INF-${\gamma}$ 12개월치료를 완료하였으나, 이들에서 UIP의 호전은 관찰되지 않았다 2) 전체 9례 중 5례가 치료 시작 후 12개월 이내에 질병악화로 인해 사망하였고, 1례는 INF-${\gamma}$ 치료종료 후 폐렴으로 사망하였다. 3) INF-${\gamma}$ 치료 시작시점에 생존군에 비하여 사망군의 폐기능이 저하되어 있었다(FVC: 추정 정상치의 $61.3{\pm}5.1$ %, vs. $45.7{\pm}12.3%$, P=0.048, $D_Lco$: $45.0{\pm}5.0$ % vs. $30.8{\pm}11.2$ %, P=0.048). 결 론 : 진행된 IPF에서 INF-${\gamma}$ 치료는 효과가 없었으며, 특히 심한 폐기능의 손상과 저산소증의 동반 시 INF-${\gamma}$의 투여는 주의를 요하는 것으로 사료되었다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1997년도 한국자동제어학술회의논문집; 한국전력공사 서울연수원; 17-18 Oct. 1997
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• pp.1460-1463
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• 1997

The aim of this paper is to suggest a design method of the model following optimal boiler-turbine H.inf. control system using genetic algorithm. This boiler-turbine H.inf. control system is designed by applying genetic algortihm with reference model to the optimal determination of weighting functions and design parameter .gamma. that are given by Glover-Doyle algornithm whch can design H.inf. contrlaaer in the sate. space. The first method to do this is ghat the gains of weightinf functions and .gamma. are optimized simultaneously by genetic algroithm. And the second method is that not only the gains and .gamma. but also the dynamics of weighting functions are optimized at the same time by genetic algonithm. The effectiveness of this boiler-turbine H.inf. control system is verified and compared with LQG/LTR control system by computer simulation.

• Archives of Pharmacal Research
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• 제28권5호
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• pp.582-586
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• 2005

The present study was designed to characterize the possible roles of spinally located cholera toxin (CTX)- and pertussis toxin (PTX)-sensitive G-proteins in pro- inflammatory cy tokine induced pain behaviors. Intrathecal injection of tumor necrosis factor-a (TNF-${\alpha}$; 100 pg), interleukin-1${\beta}$ (IL-1${\beta}$ 100 pg) and interferon-${\gamma}$ (INF-${\gamma}$; 100 pg) showed pain behavior. Intrathecal pretreatment with CTX (0.05, 0.1 and 0.5 mg) attenuated pain behavior induced by TNF-${\alpha}$ and INF-${\gamma}$ administered intrathecally. But intrathecal pretreatment with CTX (0.05, 0.1 and 0.5${\mu}g$) did not attenuate pain behavior induced by IL-1${\beta}$. On the other hand, intrathecal pretreatment with PTX further increased the pain behavior induced by TNF-${\alpha}$ and IL-1${\beta}$ administered intrathecally, especially at the dose of 0.5 ${\mu}g$. But intrathecal pretreatment with PTX did not affect pain behavior induced by INF-${\gamma}$. Our results suggest that, at the spinal cord level, CTX- and PTX-sensitive G-proteins appear to play important roles in modulating pain behavior induced by pro-inflammatory cytokines administered spinally. Furthermore, TNF-${\alpha}$, IL-1${\beta}$ arid INF-${\gamma}$ administered spinally appear to produce pain behavior by different mechanisms.

• Journal of Acupuncture Research
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• 제27권4호
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• pp.223-231
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• 2010

Objectives : The objective of this study is to study the effects of Euonymi Lignum Suberatatum pharmacopuncture solution on NO and IL-6 production in macrophage. Methods : At first, the RAW 264.7 macrophage was subclutured. In order to evaluate cytotoxicity, MTT assay performed. Then, the cell was induced by LPS, INF-$\gamma$ and Experimental groups were divided into five(Normal, Control, Euonymi Lignum Suberatatum 100, 200, $300{\mu}g/m{\ell}$). Then Euonymi Lignum Suberatatum pharmacopuncture solution was put into cell. We measured IL-6, iNOS, NO. Results : The cytotoxic effect of Euonymi Lignum Suberatatum pharmacopuncture solution in RAW 264.7 macrophage was not appeared. $300{\mu}g/m\ell$ Euonymi Lignum Suberatatum pharmacopuncture solution inhibited IL-6 production in LPS, INF-$\gamma$-stimulated RAW 264.7 macrophages significantly. Euonymi Lignum Suberatatum pharmacopuncture solution inhibited iNOS revelation in LPS, INF-$\gamma$-stimulated RAW 264.7 macrophages. All group of Euonymi Lignum Suberatatum pharmacopuncture solution inhibited NO production in LPS, INF-$\gamma$-stimulated RAW 264.7 macrophages significantly. Conclusions : Our study demonstrated that Euonymi Lignum Suberatatum pharmacopuncture solution had an inhibition effect on NO production, iNOS revelation, IL-6 production. So Euonymi Lignum Suberatatum pharmaco puncture solution may have an Anti-inflammation effect.

• 대한한방내과학회지
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• 제27권1호
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• pp.40-54
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• 2006

Objective: This study aimed to identify the different effects of GMCSBHT water-extract and ethanol-extract on Th1/Th2 differentiation by monitoring Th1/Th2 specific cytokine secretion patterns and the transcriptional activities of T-bet, GATA-3, c-maf, $INF{\gamma}$ and IL-4. Materials and Methods: Spleen cells from eight week-old BALB/c mice were cultured in GMCSBHT extracts containing medium without activation for 24 hours and with activation for 48 hours. CD4+ T cells were isolated and mRNA expression levels of $INF{\gamma}$, IL-4, T-bet, GATA-3, c-maf by RT-PCR and secretion cytokines levels of $IFN{\gamma}$, IL-4 by ELISA were analyzed. Results: GMCSBHT extracts didn't have mitogenic effects on the unstimulated CD4+ T cells. In Th1 skewed condition, GMCSBAHT water extract had no significant effects on mRNA expression levels of $IFN{\gamma}$, T-bet and c-maf, but inhibited mRNA expression levels of IL-4, GATA-3. It showed significantly increased secretion cytokine levels of $IFN{\gamma}$, but had no significant effect on secretion cytokine levels of IL-4. In Th2 skewed condition, GMCSBHT ethanol extract inhibited mRNA expression levels of $INF{\gamma}$, IL-4, GATA-3 and c-maf significantly, but had no significant effects on mRNA expression levels of T-bet. It had no significant effects on secretion cytokine levels of $INF{\gamma}$, but showed remarkable inhibitory effects on secretion cytokine levels of IL-4. Conclusion: Results suggest that on Th1/Th2 deviation, GMCSBHT water extract has both amplifying effects on Th1 differentiation and inhibitory effects on Th2, but GMCSBHT ethanol extract has stronger inhibitory effects on Th2 differentiation than on Th1.

• 대한한방내과학회지
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• 제30권1호
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• pp.64-73
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• 2009

Objectives : Consumption of fermented foods has been known to alleviate some of the symptoms of atopy and may limit allergy development, while there are also many medicinal herbs proved to be effective for immunologically-mediated diseases. In this study, we introduced modern zymology to ferment some herbs to see if fermentation has the possibility of increasing the anti-inflammatory effects of medicinal herbs. Interleukin-4 (IL-4) and interferon-gamma $(INF-\gamma)$ have been demonstrated to be the main factors in the pathology of allergic diseases. Methods : We measured the levels of IL-4 and $INF-\gamma$ on concanavalin A-induced BALB/c mice spleen cells, which were subsequently treated with fermented and unfermented herbs. We then compared the fermented groups with unfermented groups to see if the anti-inflammatory effects of the herbs were influenced by fermentation. Results and Conclusions : Our results showed that fermentation had the potential to increase the anti-inflammatory effects of some medicinal herbs, and Astragalus membranaceus and Salvia miltiorrhiza would be the most suitable medicinal herbs for fermentation among the herbs in this study.

• Tuberculosis and Respiratory Diseases
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• 제45권6호
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• pp.1167-1177
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• 1998

연구배경 : 결핵은 우리나라에서 아직도 흔하게 발생하는 질환으로 세포면역반응에 의해 육아종이 형성되며, 이 과정에서 대식세포가 분비하는 tumor necrosis factor-alpha (TNF-$\alpha$), Th1 세포가 생성하는 gamma-Interferon (INF-$\gamma$), 내피 세포가 표현하는 intercelluar adhesion molecule-1 (ICAM-1)이 중요한 역할을 할 것이라고 생각되었다. 방 법 : 저자들은 결핵의 경중(輕重)과 이러한 물질들이 관련이 있는가를 알아보기 위하여, 환자의 혈액을 채취하여 TNF-$\alpha$, INF-$\gamma$를 radioimmuno assay(RIA)로, ICAM-1이 혈액으로 유리된 형태인 sICAM-1을 enzyme linked immunosolvent assay(ELISA)로 각각 측정하였다. 또한 화학 요법에 따른 변화를 알기 위해 치료 시작후 6개월 시점에서 다시 추적검사를 실시하였다. 결 과 : TNF-$\alpha$, INF-$\gamma$, sICAM-1은 중등증과 중증의 결핵에서는 의미 있게 증가하였고, 경증에서는 의의가 없었다. 6개월간의 항결핵 치료후, sICAM-1은 임상경과에 동반하여 의미있는 감소를 보였지만. TNF-$\alpha$, INF-$\gamma$에서는 감소는 있었지만 의의는 없었다. 결 론 : 본 실험 결과 결핵의 세포 면역 매개과정에는 TNF-$\alpha$와 INF-$\gamma$가 매우 중요한 역할을 하며, 그 반응 정도가 질병의 병기에 따라 심할수록 많이 증가함을 관찰하였다. ICAM-1은 TNF-$\alpha$와 INF-$\gamma$ 농도와 비례하여 sICAM-1이 증가하였고, 질병의 병기에 따라 농도의 차이가 있을뿐 아니라, 치료경과에 비례하여 농도변화를 보여, 질병의 할동성을 나타내는 것 외에 치료 경과를 나타내는 지시자로서의 가능이 있을 것으로 생각된다.

• Journal of Acupuncture Research
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• 제24권3호
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• pp.145-161
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• 2007

Objectives : The purpose of this study is to investigate the effect of electroacupuncture at Joksanmi($ST_{36}$) on Rat's aging by experimental methods. Methods : The author performed several experimental procedures to observe the effects of the EC-HAS at the arthritis. First, I measured the cell survival rate of the mice lung fibroblasts. Second, the incidence rate of arthritis and arthritis index of CIA were analyzed. Third, the levels of IL-6, $INF-{\gamma}$ , $INF-{\alpha}$ , $IL-1{\beta}$, IgG, IgM and anti-collagen II were measured in serum and the level of $INF-{\gamma}$, $INF-{\gamma}$/IL-4 ratio in the CIA mouse spleen cell culture. Fourth, histological analysis of the mice joint was performed. Fifth, the expression ratio of $CD3e^+$ cells to $CD19^+$ cells, $CD4^+$ cells to $CD8^+$ cells, $CD11a^+CD19^+$ cells, $CD4^+CD25^+$ cells, $CD3^+CD69^+$ cells, and $CD11b^+Gr-1^+$ cells were checked. Results: 1. The hematocrit was meaningfully (p<0.01, p<0.05) decreased in $ST_{36}$ group in comparison with the control and sham group. 2. The glucose, ALP were meaningfully (p<0.05, p<0.05) decreased in $ST_{36}$ group in comparison with the control group. 3. In the activation of hepatic antioxidase, the catalase was meaningfully (p<0.01) increased in $ST_{36}$ group in comparison with the control group. 4. In the liver, the NO formation was meaningfully (p<0.001) decreased in $ST_{36}$ group in comparison with the nomal, control and sham group. 5. In the activation of splenetic antioxidase, the GSH was meaningfully (p<0.01) increased in $ST_{36}$ group in comparison with the sham group, and the catalase was meaningfully (p<0.001) increased in $ST_{36}$ group in comparison with the control group. 6. In the spleen, the MDA formation was meaningfully (p<0.01, p<0.05) decreased in $ST_{36}$ group in comparison with the control and sham group. 7. In the spleen cell culture, the levels of IL-6 was meaningfully (p<0.05) decreased in comparison with the sham group, $INF-{\gamma}$( was meaningfully (p<0.05) increased in comparison with the control group. Conclusions: According to the results, it is considered that electroacupuncture at Joksamni($ST_{36}$) is effective in increasing the activities of antioxidative enzyme and inhibiting lipid peroxides, and has an immunomodulatory reaction. In conclusion, it han an significant anti-aging effect and need more experimental study.

• 동의생리병리학회지
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• 제19권1호
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• pp.106-113
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• 2005

Asthma is an inflammatory disease of airways that is induced by Th2 cytokines and inhibited by Th1 cytokines. In this study we wanted to investigate the effect of SCT and BJT on eosinophilla and cytokines of BALF in a mouse model established airway inflammmation. Asthma was induced to male c57/bl6 mice. Allergen-specific antibody responses, cytokine$(IL-4,\;IL-5,\;INF-{\gamma})$, and eosinophil inflammation of the airways were investigated on the BALF and splenocyte. SCT and BJT effectively induced $INF-{\gamma}$ and inhibited IL-4, IL-5 as well as eosinophilic inflammation when SCT and BJT were administered. Total Ig E level in the BALF decreased. SCT was more effectiveness than BJT. It is considered that SCT and BJT have faborable effect on the asthma because the asthma specific series of abnormalities in respiratory system were decreased.

• 생명과학회지
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• 제15권4호
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• pp.652-656
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• 2005

본 연구를 통하여 규칙적인 수영 트레이닝이 체중의 감소를 일으켰으며(온수 및 냉수), 특히 냉수 훈련이 큰 감소를 나타내었다. 온수 훈련군에서는 $INF-\gamma$의 감소를 유발하여 림프구의 증식을 Th0에서 Th2 쪽으로 유도한다는 사실을 알 수 있었으며, 이로 인해 결핵균을 감염시켰을 경우 오히려 폐와 비장에서 결핵균의 수가 현저히 증가하는 사실을 알 수 있어, 수영 훈련군에게서의 결핵균 감염 시 대조군에 비해 균수가 현저히 증가함을 알 수 있었다. 하지만 냉수 훈련군에서는 통제군에 비해 $INF-\gamma$의 현저한 증가를 보여, 결핵균 감염 시 폐에서 결핵균 수가 감소함을 확인 할 수 있었다.