• Journal of Life Science
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• v.31 no.12
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• pp.1079-1087
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• 2021

To analyze gut microbiota of livestock in Korea and compare taxonomic differences, we conducted 16S rRNA metagenomic analysis through next-generation sequencing. Fecal samples from broiler chickens, pigs, and cattle were collected from domestic feedlots randomly. α-diversity results showed that significant differences in estimated species richness estimates (Chao1 and ACE, Abundance-based coverage estimators) and species richness index (OUTs, Operational taxonomic units) were identified among the three groups. However, NPShannon, Shannon, and Simpson indices revealed that abundance and evenness of the species were statistically significant only for poultry (broiler chickens) and mammals (pigs and cattle). Firmicutes was the most predominant phylum in the three groups of fecal samples. Linear discriminant (LDA) effect size (LEfSe) analysis was conducted to reveal the ranking order of abundant taxa in each of the fecal samples. A size-effect over 2.0 on the logarithmic LDA score was used as a discriminative functional biomarker. As shown by the fecal analysis at the genus level, broiler chickens were characterized by the presence of Weissella and Lactobacillus, as well as pigs were characterized by the presence of provetella and cattele were characterized by the presence of Acinetobacter. A permutational multivariate analysis of variance (PERMANOVA) showed that differences of microbial clusters among three groups were significant at the confidence level. (p=0.001). This study provides basic data that could be useful in future research on microorganisms associated with performance growth, as well as in studies on the livestock gut microbiome to increase productivity in the domestic livestock industry.

• Journal of Korean Society of Forest Science
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• v.95 no.3
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• pp.334-341
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• 2006

This study was to investigate the growth of planted red pine (Pinus densiflora S. et. Z.) seedling, soil properties and understory vegetation structure after fertilizer treatments [unfertilized plot (control), CF plot (Combination Fertilizer), UF plot (Urea Formaldehyde Fertilizer)] in a Pinus densiflora stand planted after the forest fires in Gosung, Gangwon province. The height growth rates of seedlings in four years were 264% in unfertilized, 404% in CF, and 388% in UF plots, respectively. The root collar diameters were increased 340% in unfertilized, 454% in CF, and 427% in UF plots, respectively. No significant changes occurred in soil total nitrogen and potassium ion ($K^+$) with the fertilization. However, available $P_2O_5$, content in the soil surface (0-15 cm) increased with the fertilizer application. Soil organic matter increased significantly with fertilizer treatments, while gradual decrease occurred in unfertilized plots. Sodium ion ($Na^-$) decreased in all sites. Soil pH, CEC, calcium ion ($Ca^{2+}$) and magnesium ion ($Mg^{2+}$) contents were not significantly different among treatments. Although Shannon's species diversity index and species richness in understory vegetation did not change with fertilizer treatments, vegetation cover rates in forest floor increased significantly with the fertilization. These results suggest that the increase of pine seedling growth and vegetation cover rates with fertilization could enhance soil stabilization in forest tire areas.

• Korean Journal of Environmental Biology
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• v.25 no.4
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• pp.385-393
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• 2007

Benthic macroinvertebrate communities were studied after a small dam removal from the mid-section of the Gyeongan stream in Gyeonggi-do, Korea. Quantitative sampling was conducted at immediately upstream (upper) and downstream (lower) sites from the dam as well as at the site where the dam was located (dam site: middle) using a Surber sampler (50$\times$50 cm, mesh 0.25 mm), four times (November 2004, May 2005, January 2006, and May 2006) after the dam removal. As a result, 46 species of benthic macroinvertebtates, belonged in 35 genera, 27 families, 11 orders, 5 classes, and 4 phyla, were sampled from the stream sites, but the number of species that occurred at each sampling trial was different (ranged 3$\sim$17 spp.) according to the seasons and sites. Approximately one year after the dam removal, the species number has in-creased and taxa composition has changed as the microhabitat became more heterogeneously due to a riffle formation in the upstream site. Chironomid larvae and tubificid worms, which are common in Korean urban streams, were the dominant species, while Hydropsyche kozhantschikovi was the 2nd dominant species at some sampling trials. In general, McNaughton's dominance indices decreased and Shannon species diversity indices increased approximately one year after the dam removal. Compositions of collector-filterers, clingers, and swimmers increased as hydropsychid caddisflies, heptageniid mayflies, and baetid mayflies increased, respectively, in the upstream site. The group pollution index and the ecological score using benthic macroinvertebrates both indicated that water environment has been improved in the upstream site after the dam removal.

• Journal of the Korean Institute of Landscape Architecture
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• v.47 no.3
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• pp.81-92
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• 2019

This study was conducted to establish vegetation structure characteristics and vegetation management plan of the cultural landscape forests located around Unmun Temple in Cheongdo-gun and to provide the basic data needed to manage the cultural landscape forests. The landscape characteristics are analyzed in two perspectives including the landscape as viewed from inside and outside of Unmun Temple and eight landscape views are selected. Main views are Pinus densiflora forest and Abies holophylla forest around Unmun Temple. As a result of the survey of existing vegetation around Cheongdo-gun Unmun Temple, P. densiflora forests and P. densiflora-A. holophylla forests are widely distributed, occupying 79.2% of the forest. The plant community structure was classified into seven types according to the three topographic characteristics, flat forests, slope forests, and lower forests, Which were divided into a total of 30 survey plots and the average relative importance percentage was determined. The P. densiflora community on the flat are dominated by Carpinus tschonoskii in Under-canopy. The P. densiflora-A. holophylla community on the flat had a relatively high rate of domination in the shrubs. There were no competing species for the A. holophylla community on the plat. The large standard P. densiflora and the small standard P. densiflora were expected to be confined by P. serrulata var. pubescens and the Quercus variabilis on the slopes. The managed P. densiflora community had a relatively high rate of P. densiflora domination in the shrubs. The P. densiflora community on the lowland was dominated by Styrax japonicus and P. serrulata var. pubescens. The Shannon species diversity index was 0.2360 to 1.4088. The results of the correlation analysis with P. densiflora, A. holophylla and other species were P. densiflora had negative correlation with Acer mono, Corylus heterophylla var. heterophylla, Zelkova serrata and A. holophylla, and A. holophylla have negative correlation with S. japonicus and P. densiflora. Landscape characteristics and plant community structures are analyzed to propose management methods of maintaining and restoring The P. densiflora and A. holophylla cultural forest landscapes around Unmun Temple.