• Journal of Microbiology and Biotechnology
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• v.22 no.2
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• pp.234-238
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• 2012

Recombinant Escherichia coli displaying organophosphorus hydrolase (OPH) was used to overcome the diffusion barrier limitation of organophosphorus pesticides. A new anchor system derived from the N-terminal domain of ice-nucleation protein from Pseudomonas syringae InaV (InaV-N) was used to display OPH onto the surface. The designed sequence was cloned in the vector pET-28a(+) and then was expressed in E. coli. Tracing of the expression location of the recombinant protein using SDS-PAGE showed the presentation of OPH by InaV-N on the outer membrane, and the ability of recombinant E. coli to utilize diazinon as the sole source of energy, without growth inhibition, indicated its significant activity. The location of OPH was detected by comparing the activity of the outer membrane fraction with the inner membrane and cytoplasm fractions. Studies revealed that recombinant E. coli can degrade 50% of 2 mM chlorpyrifos in 2 min. It can be concluded that InaV-N can be used efficiently to display foreign functional protein, and these results highlight the high potential of an engineered bacterium to be used in bioremediation of pesticide-contaminated sources in the environment.

• Proceedings of the Korea Crystallographic Association Conference
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• 2005.11a
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• pp.19-19
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• 2005

• Journal of the Korean housing association
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• v.19 no.6
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• pp.137-144
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• 2008

Public housing problem became a major issue in each European country during the period of the reconstruction. This study focuses on the one of the major institutional policies in the public housing sectors in Europe during the second postwar period, specifically the INA (Istituto Nazionale delle Assicurazioni)-Casa in Italy from 1949 to 1963. Thanks to the financial Aid provided by 'Marshall Plan' of United States government, Italy initiated INA-Casa housing program elaborated and realized by minister of Labour, Amintore Fanfani. He was strongly influenced by catholic socialism and Weberian corporatism, and also had previously written theoretical studies on the subject during the Interwar years. The outcome of this policy had been devaluated for its Keynesian aspect as well as vernacular predominance in the architectural forms. On the contrary, this study focuses on themes such as continuity of politics before and after the Liberation, status of the architects and their will to social engagement, notion of quarter as 'self-sufficient community' and 'residential unit'. These factors will allow to comprehend the complex nature of the reality where different categories were involved to resolve the common housing problem.

• The Korean Journal of Archival Studies
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• no.58
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• pp.95-145
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• 2018

As a communication language, non-text records and archives such as photographs, images, and videos are becoming more important than text records. As a result, domestic institution, organizations, and specialists related to record management have been emphasizing the necessity of the archive management system appropriate for the distinctive characteristics of image records. This paper summarizes the points to be considered for the establishment of the Korean audiovisual archives management system, based on the writer's experience of participating in the International Audiovisual Archives Management Training for professionals in the world(INA Frame) of 2018. In particular, various types of contents including cinema, broadcasting, cultural should be managed at the national level. Furthermore, the necessity of a new concept establishment for "public audiovisual heritage" is accentuated. In addition, the tasks regarding the establishment of foundation, such as the modification of the related systems and infrastructures, and the layouts of the institution or governance, should be reviewed and revised. Moreover, the preliminary tasks revised, should be lead to the establishment of stable management system for public audiovisual archives of Korea.

• SUVANNABHUMI
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• v.1 no.1
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• pp.135-143
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• 2009

• Restorative Dentistry and Endodontics
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• v.21 no.1
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• pp.425-435
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• 1996

The effect of application of dentin bonding agent to the exposed dentin on the intradental nerve activity (INA), dentinal fluid movement and sealing of the dentinal tubules, was investigated in this study. The INA was recorded from the single pulp nerve unit dissected from the inferior alveolar nerve. And specimen of dentin was observed by SEM. Dentinal fluid 'movement through exposed dentin surface was measured before and after the application of dentin bonding agent. 1. Eight Ao-fiber units (conduction velocity: $8.0{\pm}4.0m$/sec) were identified. 4M NaCl evoked an irregular burst of action potentials which ceased immediately after washing. 2. In 4 $A{\delta}$-fiber units, appliction of All Bond 2 completely abolished the INA induced by 4M NaCl. Also, application of Scotchbond Multipurpose(SBMP) totally abolished the INA induced by 4M NaCl in 4 $A{\delta}$-fiber units. 3. Before the application of dentin bonding agent, outward dentinal fluid movement of $10.2{\pm}5.7\;pl{\cdot}s^{-1}{\cdot}mm^{-2}$ was obsered. But after the application of dentin bonding agent the movement of dentinal fluid was stopped. 4. The gap width of 2-$3{\mu}m$ was formed between exposed dentin and adhesive resin in the specimens applied with dentin bonding agents of All Bone 2 and SBMP. But the formation of hybrid layer and the penetration of resin into were dentinal tubules were not clearly observed in interface between dentin and adhesive resin.

• Restorative Dentistry and Endodontics
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• v.21 no.2
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• pp.505-516
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• 1996

The effects of application of dentin desensitizer containing glutaraldehyde (Gluma Desensitizer) and dentin adhesive system (All Bond 2) to the exposed dentin on the intradental nerve activity (INA) and the occluding aspects of dentinal tubules were investigated in cat canine teeth. Single pulp nerve units were dissected from the inferior alveolar nerve and indentified as $A{\delta}$-fiber units. The INAs elicited by 4M NaCl before and after the application of each experimental agent were compared. The morphological changes of exposed dentin surfaces and dentinal tubules in the dentin specimens used to record INAs were observed by SEM. The results obtained were as follows. 1. Eight $A{\delta}$-fiber units (conduction velocity: $8.0{\pm}4.0m$/sec) were identified. 4M NaCl evoked an irregular burst of action potentials which ceased immediately after washing. 2. In 4 $A{\delta}$-fiber units, the change of INA after the application of Gluma Desensitizer was $133.9{\pm}80.7%$ when it was compared with the INA before the application of the same agent. 3. In 4 $A{\delta}$-fiber units, application of All Bond 2 completely abolished the INA induced by 4M NaCl. 4. In specimens applied with Gluma Desensitizer, the formation of hybrid layer as well as the identification of resin penetration and reaction products with proteins in dentinal tubules were not clearly observed in interface between dentin and adhesive resin. In specimens applied with All Bond 2, the gap width of 2-$3{\mu}m$ was formed between exposed dentin and adhesive resin, and the hybrid layer and resin tags were not clearly formed either.

• Journal of agriculture & life science
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• v.46 no.4
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• pp.113-118
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• 2012

Effects of plant activators on germination of primed pepper seeds were investigated. The percent germination and T50 of primed seeds with 0.1mM ASM were 98% and 0.96 day at the 7 days after seeding, respectively. However, the germination of primed seeds with 0.5mM ASM were suppressed as 17% at the 7 days after seeding. The percent germination of primed seeds with 0.01mM INA were 90% at the 2 days after seeding. However, those of primed seeds by 0.1mM INA were increased as 90% at the 5 days after seeding. The percent germination of primed seeds was not change by treatment of the BABA and JA.

• Journal of Sericultural and Entomological Science
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• v.20 no.2
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• pp.32-35
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• 1978

The infectious flacherie viruses are serious diseases in the silkworm, which affects the cocoon crops. However, there is only a few study on this diseases up-to-date in Korea and, in this experiment, the authors investigated the resistance of the silkworm varieties to flacherie and Ina-flacherie viruses by peroral infection. A cross, Hansaeng ＃1$\times$ Harisaeng ＃2, showed the highest resistance to the flacherie virus a Mudeung$\times$Geumho showed the lowest resistance among the examined varieties. It seemed that the varietal difference of resistance against Ina-flacherie virus was appeared and Jam117$\times$ Jam118, Gyeongchu$\times$Yeonil, Mudeung$\times$Geumho, Hansaeng＃1$\times$Hansaeng＃2 and Hansaeng＃3 $\times$ Hansaeng ＃4 showed non susceptibility by peroral infection. However it was shown that Jam115 $\times$Jam116 had the lowest resistance to Ina-flacherie virus.

• Membrane and Water Treatment
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• v.13 no.6
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• pp.321-335
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• 2022

Copper-based Metal-organic framework (MOF) namely ([Cu (INA)₂]-MOF) is synthesized by ball milling and characterized using scanning electron microscopy (SEM) for the topography, microstructure, and elemental evidence determination, powdered X-ray diffraction (XRD) for the crystallinity measurement, thermogravimetric (TG) analysis was performed to determine the thermal stability of the material, and Fourier transformed infrared (FTIR) spectroscopy for functional groups identification. The use of [Cu (INA)₂]-MOF as hazardous removal material of β-agonists as persistent hazardous micro-pollutants in our environmental water is first reported in this study. The removal efficiency of the Cu-MOF is successfully determined to be 97.7% within 40 minutes, and the MOF has established an exceptional removal capacity of 835 mg L^-1 with 95 % percent removal on Clenbuterol (CLB) even after the 5th consecutive cycle. The Langmuir model of the adsorption isotherms was shown to be more favourable, while the pseudo-second-order model was found to be favoured in the kinetics. The reaction was exothermic and spontaneous from a thermodynamic standpoint, and the higher temperatures were unfavourable for the adsorption study of the CLB. As a result, the studied MOF have shown promising properties as possible adsorbents for the removal of CLB in wastewater.