• Title/Summary/Keyword: IL-17F

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Effect of Hardening and Abscisic Acid Treatments at Seedling Stage on Chilling Injury and Related Physiological Responses in Rice Plants (수도(水稻)의 냉해(冷害)와 생리적(生理的) 반응(反應)에 미치는 유묘(幼苗)의 경화(硬化) 및 Abscisin 산(酸) 처리효과(處理效果))

  • Lee, Byoung-Moo;Ryu, In-Soo;Hur, Il-Bong
    • Korean Journal of Soil Science and Fertilizer
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    • v.22 no.1
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    • pp.61-66
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    • 1989
  • Plants were exposed to different sub-optimal growth temperature regimes for hardening or sprayed with abscisic acid (ABA) before being placed at $10^{\circ}C$ for three days. Comparisons were made with non-treated plants for plant survival, photosynthetic activities and fatty acids composition of phospholipids. The level of endogenous ABA of the hardened seedlings was compared with that in control and ABA sprayed-seedlings. The results of the above experiment were summarized as follows: 1. Compared to non-treated plants, the hardening and ABA treatment resulted in higher plant survival rate and better seedling growth following earlier transplanting. The low temperature hardening which gradually increased the duration of the low temperature treatment at 2-day intervals was more effective than the controled temperature hardening which gradually decreased the temperature from $25^{\circ}C$ to $20^{\circ}C$ and $15^{\circ}C$. 2. The photosynthetic activities of rice leaves after 3-day exposure to $10^{\circ}C$ were higher in hardened and ABA sprayed seedlings than in non-treated plants. The root activities after low temperature treatment were also higher in hardened and ABA sprayed seedlings than in control. 3. Fatty acids of phospholipids in rice plants such as stearic acid and oleic acid were decreased and the highly unsaturated fatty acids, linoleic acid and lenolenic acid were increased by hardening or ABA treatments. It is assumed that the increase of cold tolerance in these plants was due to the higher ratio of unsaturated to saturated fatty acids. Respectively, the ratio was 1.86, 1.97 and 1.80 in plants given controled temperature hardening, the low temperature hardening and ABA treatments. While in control plants, it was 1.17. 4. ABA content in rice seedling given the low temperature hardening treatment was 67.2 ng/g F.W which was approximately twice that of non-treated control, which was 33.9 ng/f F. W., in contrast, the ABA content of the ABA treated seedlings was 91.7 ng/g F. W.

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Design of a 2.4-GHz Fully Differential Zero-IF CMOS Receiver Employing a Novel Hybrid Balun for Wireless Sensor Network

  • Chang, Shin-Il;Park, Ju-Bong;Won, Kwang-Ho;Shin, Hyun-Chol
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.8 no.2
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    • pp.143-149
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    • 2008
  • A novel compact model for a five-port transformer balun is proposed for the efficient circuit design of hybrid balun. Compared to the conventional model, the proposed model provides much faster computation time and more reasonable values for the extracted parameters. The hybrid balun, realized in $0.18\;{\mu}m$ CMOS, achieves 2.8 dB higher gain and 1.9 dB lower noise figure than its passive counterpart only at a current consumption of 0.67 mA from 1.2 V supply. By employing the hybrid balun, a differential zero-IF receiver is designed in $0.18\;{\mu}m$ CMOS for IEEE 802.15.4 ZigBee applications. It is composed of a differential cascode LNA, passive mixers, and active RC filters. Comparative investigations on the three receiver designs, each employing the hybrid balun, a simple transformer balun, and an ideal balun, clearly demonstrate the advantages of the hybrid balun in fully differential CMOS RF receivers. The simulated results of the receiver with the hybrid balun show 33 dB of conversion gain, 4.2 dB of noise figure with 20 kHz of 1/f noise corner frequency, and -17.5 dBm of IIP3 at a current consumption of 5 mA from 1.8 V supply.

Development of PCR-Based Sequence Characterized DNA Markers for the Identification and Detection, Genetic Diversity of Didymella bryoniae with Random Amplified polymorphic DNA(RAPD)

  • Kyo, Seo-Il;Shim, Chang-Ki;Kim, Dong-Kil;Baep, Dong-Won;Lee, Seon-Chul;Kim, Hee-Kyu
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.130-130
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    • 2003
  • Gummy stem blight pathogen is very difficult not only to monitor the inoculum levels prior to host infection, and also it is destructive and hard to control in field condition. We have applied RAPD technique to elucidate the genetic diversity of the genomic DNA of Didymella bryoniae and also to generate specific diagnostic DNA probe useful for identification and detection. The 40 primers produced clear bands consistently from the genomic DNA of twenty isolates of Didymella bryoniae, and two hundred seventy-three amplified fragments were produced with 40 primers. The combined data from 273 bands was analyzed by a cluster analysis using UPGMA method with an arithmetic average program of NTSYS-PC (Version 1.80) to generate a dendrogram. At the distance level of 0.7, two major RAPD groups were differentiated among 20 strains. RAPD group (RG) I included 8 isolates from watermelon except one isolate from melon. RAPD group (RG) IV included 12 isolates from squash, cucumber, watermelon and melon.. In amplification experiment with SCAR specific primer RG1F-RG1R resulted in a single band of 650bp fragment only for 8 isolates out of 20 isolates that should be designated as RAPD Group 1. However, same set of experiment done with RGIIF-RGIIR did not result in any amplified product.. Our attempts to detect intraspecific diversity of ITS region of rDNA by amplifying ITS region and 17s rDNA region for 20 isolates and restriction digestion of amplified fragment with 12 enzymes did not reveal polymorphic band. In order to develop RAPD markers for RGIV specific primer, a candidate PCR fragment( ≒1.4kb) was purified and Southern hybridized to the amplified fragment RGIV isolates. This promising candidate probe recognized only RGIV isolates

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Performance Measurement of SMT(Slewing Mirror Telescope) Optical System

  • Ahn, Ki-Beom;Jeong, Soo-Min;Kim, Ji-Eun;Kim, Sug-Whan;Lee, Jik;Lim, Heui-Jin;Lindere, V.;Nam, Ji-Woo;Nam, Koo-Hyun;Park, Il-H.;Smoot, G.F.
    • Bulletin of the Korean Space Science Society
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    • 2011.04a
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    • pp.23.1-23.1
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    • 2011
  • The SMT is a subsystem of the UFFO (Ultra-Fast Flash Observatory) pathfinder onboard the Lomonosov spacecraft planed to be launched in November 2011. The UFFO is designed for extremely fast observation of optical afterglow of Gamma Ray Burst (GRB). This study is primarily concerned with performance measurement of the SMT optical system under the integration and test phase. SMT is a 100mm Ritchey-Chretien type telescope with a motorized slewing mirror and a $256{\times}256$ pixels Intensified Charge-Coupled Device (ICCD) of 22.2${\mu}m$ in pixel size. SMT is designed to operate over the wavelength coverage between 200 nm and 650 nm. It has 17 arcmin FOV (Field of View), providing 4arcsec in detector pixel resolution. In this study, we describe the integration and test process of the SMT optical system and interim performance measurement results with motorized slewing mirror and ICCD.

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The Sensory Evaluation and Practical Use of Kochujang Sauce Adopted to Home Use Test (Home Use Test를 적용한 고추장 소스의 관능 및 활용도 평가)

  • Lim, Seong-Il;Han, Kyung-Soo;Cho, Gyeong-Hyeon;Seo, Kyung-Mi
    • Korean journal of food and cookery science
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    • v.24 no.6
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    • pp.771-779
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    • 2008
  • The purpose of the study was assessing Kochujang sauces(BBQ & Chicken) using a Home Use Test(HUT) focused on England food consumer. To achieve of the purpose, 58 England food consumers were selected by CCFRA's customer database. The qualification of the sample was responsible for the households' main grocery shopping, cooking Oriental/ Far Eastern cuisines at home, enjoying hot/spicy chilli based food and would be willing to buy hot chilli based Oriental sauce. 3 times of HUT were accomplished. The test was continued during 3 weeks, and each test was continued during one week. To check the sensory evaluation results on these sauces, 9 point hedonic scale and JAR(Just About Right) were used. The frequency analysis was adopted for the study. The results of a study were as followed. The overall satisfaction(upper 7point) on BBQ sauce was 60%, and the chicken sauce was 55.1%. The sweetness, hot taste, and BBQ aroma were suitable as a upper 40% using JAR scale. On purchase intention, BBQ & Chicken was slightly increased. These sauces were proper lunch & Dinner time. Also, good for dip, stir fry, and marinade. The result of put to use the sauces on food material as followed. BBQ sauce was proper Chicken(45.5%), vegetable(29.9%), and turkey(19%) in the order named. In case of the chicken sauce was ordered chicken(46.2%), vegetable(27.5%), beef(17.5%).

The importance of post-thaw subculture for standardizing cellular activity of fresh or cryopreserved mouse embryonic stem cells

  • Ko, Dong Woo;Yoon, Jung Ki;Ahn, Jong il;Lee, Myungook;Yang, Woo Sub;Ahn, Ji Yeon;Lim, Jeong Mook
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.3
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    • pp.335-343
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    • 2018
  • Objective: Remarkable difference in cellular activity was found between early and late subpassaged embryonic stem cell (ESCs) lines, which can be created by subtle changes in cell manipulation protocol. This study subsequently examined whether post-thaw subculture of early subpassaged ESC lines could further affect the activity of the ESCs. Methods: Fresh (as a control treatment) or cryopreserved F1 hybrid (B6CBAF1) early ESC lines (C57BL/6xCBA) of the 4 (P4) or the 19 passage (P19) were subcultured once, twice or six times under the same condition. The post-thaw survival of the ESCs was monitored after the post-treatment subculture and the ability of cell proliferation, reactive oxygen species (ROS) generation, apoptosis and mitochondrial ATP synthesis was subsequently examined. Results: Regardless of the subculture number, P19 ESCs showed better (p<0.05) doubling time and less ATP production than P4 ESCs and such difference was not influenced by fresh or cryopreservation. The difference between P4 and P19 ESC lines became decreased as the post-treatment subculture was increased and the six times subculture eliminated such difference. Similarly, transient but prominent difference in ROS production and apoptotic cell number was detected between P4 and P19 ESCs only at the 1st subculture after treatment, but no statistical differences between two ESC lines was detected in other observations. Conclusion: The results of this study suggest that post-thaw subculture of ESCs under the same environment is recommended for standardizing their cellular activity. The activity of cell proliferation ability and ATP synthesis can be used as parameters for quality control of ESCs.

On Constructing NURBS Surface Model from Scattered and Unorganized 3-D Range Data (정렬되지 않은 3차원 거리 데이터로부터의 NURBS 곡면 모델 생성 기법)

  • Park, In-Kyu;Yun, Il-Dong;Lee, Sang-Uk
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.37 no.3
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    • pp.17-30
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    • 2000
  • In this paper, we propose an efficient algorithm to produce 3-D surface model from a set of range data, based on NURBS (Non-Uniform Rational B-Splines) surface fitting technique. It is assumed that the range data is initially unorganized and scattered 3-D points, while their connectivity is also unknown. The proposed algorithm consists of three steps: initial model approximation, hierarchical representation, and construction of the NURBS patch network. The mitral model is approximated by polyhedral and triangular model using K-means clustering technique Then, the initial model is represented by hierarchically decomposed tree structure. Based on this, $G^1$ continuous NURBS patch network is constructed efficiently. The computational complexity as well as the modeling error is much reduced by means of hierarchical decomposition and precise approximation of the NURBS control mesh Experimental results show that the initial model as well as the NURBS patch network are constructed automatically, while the modeling error is observed to be negligible.

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Personalized Diabetes Risk Assessment Through Multifaceted Analysis (PD- RAMA): A Novel Machine Learning Approach to Early Detection and Management of Type 2 Diabetes

  • Gharbi Alshammari
    • International Journal of Computer Science & Network Security
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    • v.23 no.8
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    • pp.17-25
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    • 2023
  • The alarming global prevalence of Type 2 Diabetes Mellitus (T2DM) has catalyzed an urgent need for robust, early diagnostic methodologies. This study unveils a pioneering approach to predicting T2DM, employing the Extreme Gradient Boosting (XGBoost) algorithm, renowned for its predictive accuracy and computational efficiency. The investigation harnesses a meticulously curated dataset of 4303 samples, extracted from a comprehensive Chinese research study, scrupulously aligned with the World Health Organization's indicators and standards. The dataset encapsulates a multifaceted spectrum of clinical, demographic, and lifestyle attributes. Through an intricate process of hyperparameter optimization, the XGBoost model exhibited an unparalleled best score, elucidating a distinctive combination of parameters such as a learning rate of 0.1, max depth of 3, 150 estimators, and specific colsample strategies. The model's validation accuracy of 0.957, coupled with a sensitivity of 0.9898 and specificity of 0.8897, underlines its robustness in classifying T2DM. A detailed analysis of the confusion matrix further substantiated the model's diagnostic prowess, with an F1-score of 0.9308, illustrating its balanced performance in true positive and negative classifications. The precision and recall metrics provided nuanced insights into the model's ability to minimize false predictions, thereby enhancing its clinical applicability. The research findings not only underline the remarkable efficacy of XGBoost in T2DM prediction but also contribute to the burgeoning field of machine learning applications in personalized healthcare. By elucidating a novel paradigm that accentuates the synergistic integration of multifaceted clinical parameters, this study fosters a promising avenue for precise early detection, risk stratification, and patient-centric intervention in diabetes care. The research serves as a beacon, inspiring further exploration and innovation in leveraging advanced analytical techniques for transformative impacts on predictive diagnostics and chronic disease management.

Ginseng root-derived exosome-like nanoparticles protect skin from UV irradiation and oxidative stress by suppressing activator protein-1 signaling and limiting the generation of reactive oxygen species

  • Wooram Choi;Jeong Hun Cho;Sang Hee Park;Dong Seon Kim;Hwa Pyoung Lee;Donghyun Kim;Hyun Soo Kim;Ji Hye Kim;Jae Youl Cho
    • Journal of Ginseng Research
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    • v.48 no.2
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    • pp.211-219
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    • 2024
  • Background: Recently, plant-derived exosome-like nanoparticles (PDENs) have been isolated, and active research was focusing on understanding their properties and functions. In this study, the characteristics and molecular properties of ginseng root-derived exosome-like nanoparticles (GrDENs) were examined in terms of skin protection. Methods: HPLC-MS protocols were used to analyze the ginsenoside contents in GrDENs. To investigate the beneficial effect of GrDENs on skin, HaCaT cells were pre-treated with GrDENs (0-2 × 109 particles/mL), and followed by UVB irradiation or H2O2 exposure. In addition, the antioxidant activity of GrDENs was measured using a fluorescence microscope or flow cytometry. Finally, molecular mechanisms were examined with immunoblotting analysis. Results: GrDENs contained detectable levels of ginsenosides (Re, Rg1, Rb1, Rf, Rg2 (S), Gyp17, Rd, C-Mc1, C-O, and F2). In UVB-irradiated HaCaT cells, GrDENs protected cells from death and reduced ROS production. GrDENs downregulated the mRNA expression of proapoptotic genes, including BAX, caspase-1, -3, -6, -7, and -8 and the ratio of cleaved caspase-8, -9, and -3 in a dose-dependent manner. In addition, GrDENs reduced the mRNA levels of aging-related genes (MMP2 and 3), proinflammatory genes (COX-2 and IL-6), and cellular senescence biomarker p21, possibly by suppressing activator protein-1 signaling. Conclusions: This study demonstrates the protective effects of GrDENs against skin damage caused by UV and oxidative stress, providing new insights into beneficial uses of ginseng. In particular, our results suggest GrDENs as a potential active ingredient in cosmeceuticals to promote skin health.

Quantitative Evaluation on Optimal Scan Time of PET/CT Studies Using TOF PET (TOF 기법을 이용한 PET/CT 검사에서 적정 스캔 시간에 대한 정량적 평가)

  • Moon, Il-Sang;Lee, Hong-Jae;Kim, Jin-Eui;Kim, Hyun-Joo
    • The Korean Journal of Nuclear Medicine Technology
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    • v.16 no.1
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    • pp.34-37
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    • 2012
  • Purpose: To verify the optimal scan time per bed for clinical application, we evaluated the quality of $^{18}F$-FDG images with varying scan times in a phantom and 20 patients with 38 lesions using a Philips (TOF) PET/CT scanner. Materials and Methods: The PET/CT images of a NEMA IEC body phantom and 20 patients (16 males, 4 females) were acquired for 5 different scan times of 20-100 sec per bed with intervals of 20 sec. The activity ratio of hot spheres (diameter of 17 [H1], 22 [H2] and 28 [H3] mm) to the background region in the IEC body phantom was 8-to-1. The contrast recovery coefficient (CRC) and standard uptake value (SUV) based on ROIs of hot spheres and background region were calculated. The noise in each background region was estimated as the ratio of SD of counts to the mean counts in the background region. On the patient image, the injected dose of $^{18}F$-FDG was $444{\pm}74$ MBq and the SUVs in the 38 hot lesions were measured. Results: The two scan time groups (LT-60 [<60 sec] and GT-60 [${\geq}60$ sec]) were compared. In the phantom study, the coefficient of deviations (CVs, %) of CRC and SUV in LT-60 (H1: 14.2 and 7.3, H2: 11.4 and 7.8, H3: 4.9 and 3.2) were higher than GT-60 (H1: 8.9 and 2.8, H1: 8.2 and 5.0, H3: 2.0 and 1.6). In the patient study, the mean CV of CRC and SUV in LT-60 (4.0) was higher than GT-60 (1.2). Conclusion: This study showed that noise increased as the scan time decreased. High noise for the scan time <60 sec per bed yielded high variation of SUV and CRC. Therefore, considering PET/CT image quality, the scan time per bed in the TOF PET/CT scanner should be at least ${\geq}60$ sec.

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