• Radiation Oncology Journal
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• 제37권1호
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• pp.37-42
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• 2019

Purpose: To identify prognostic factors influencing progression-free survival (PFS) of aggressive fibromatosis (AF) after postoperative radiotherapy (PORT) and assess correlations between immunohistochemistry (IHC) features of β-catenin/smooth muscle actin (SMA) and PFS. Materials and Methods: Records of 37 patients with AF treated by PORT from 1984 to 2015 were retrospectively reviewed. Fifteen patients underwent wide excision for AF and 22 patients received debulking operation. The median total dose of PORT was 59.4 Gy. IHC staining results of β-catenin and SMA were available for 11 and 12 patients, respectively. Results: The median follow-up duration was 105.9 months. Five-year PFS rate was 70.9%. Tumor size or margin status was not related to PFS in univariate analysis (p = 0.197 and p = 0.716, respectively). Multivariate analysis showed that increased interval from surgery to PORT (>5.7 weeks) was a marginal risk factor for PFS (p = 0.054). Administration of PORT at the initial diagnosis resulted in significantly improved PFS compared to deferring PORT after recurrence (p = 0.045). Patient with both risk factors of deferring PORT after recurrence and interval from surgery to PORT >5.7 weeks had significantly lower 5-year PFS than patients without risk factor (34.1% vs. 100.0%; p = 0.012). Nuclear β-catenin intensity tended to inversely correlate with 5-year PFS, although it did not reach statistical significance (62.5% at low vs. 100.0% at high; p = 0.260). SMA intensity was not related to PFS (p = 0.700). Conclusion: PORT should be performed immediately after surgery irrespective of margin status or tumor size especially in recurrent case. Nuclear β-catenin staining intensity of IHC might correlate with local recurrence.

• IMMUNE NETWORK
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• 제11권2호
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• pp.107-113
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• 2011

Background: Metabolic disorders, including type II diabetes and obesity, present major health risks in industrialized countries. AMP-activated protein kinase (AMPK) has become the focus of a great deal of attention as a novel therapeutic target for the treatment of metabolic syndromes. In this study, we evaluated whether dietary aloe could reduce obesity-induced inflammation and adipogenesis. Methods: Male C57BL/6 obese mice fed a high-fat diet for 54 days received a supplement of aloe formula (PAG, ALS, Aloe QDM, and Aloe QDM complex) or pioglitazone (PGZ) and were compared with unsupplemented controls (high-fat diet; HFD) or mice fed a regular diet (RD). RT-PCR and western blot analysis were used to quantify the expression of obesity-induced inflammation. Results: Aloe QDM complex downregulated fat size through suppressed expression of scavenger receptors on adipose tissue macrophages (ATMs) compared with HFD. Both white adipose tissue (WATs) and muscle exhibited increased AMPK activation through aloe supplementation, and in particular, the Aloe QDM complex. Obesity-induced inflammatory cytokines (IL-$1{\beta}$ and -6) and $HIF1{\alpha}$ mRNA and protein were decreased markedly, as was macrophage infiltration by the Aloe QDM complex. Further, the Aloe QDM complex decreased the translocation of NF-${\kappa}B$ p65 from the cytosol in the WAT. Conclusion: Dietary aloe formula reduced obesity-induced inflammatory responses by activation of AMPK in muscle and suppression of proinflammatory cytokines in the WAT. Additionally, the expression of scavenger receptors in the ATM and activation of AMPK in WAT led to reduction in the percent of body fat. Thus, we suggest that the effect of the Aloe QDM complex in the WAT and muscle are related to activation of AMPK and its use as a nutritional intervention against T2D and obesity-related inflammation.

• 한국균학회지
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• 제48권2호
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• pp.125-133
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• 2020

국내 표고의 고품질 톱밥재배용 품종을 육성하기 위해 수집균주 및 우량계통의 단포자 교배를 통해 육성된 '화담'의 주요 특성은 다음과 같다. 균사생장 적온은 25℃, 발이 및 생육온도는 12-20℃, 형태는 평반구형으로 '산조701호'와 유사하였으며, 발생형은 '산조701호'가 집중형인데 비해 '화담'은 산발형으로 나타났다. '화담'의 재배특성 조사결과 배양일수는 35일, 갈변기간은 84일로 '산조701호'과 유사하였고, 생육일수는 10일로 '산조701호'보다 2일 늦어 전체 재배일수도 129일로 '산조701호'의 127일에 비해 2일 늦었다. '화담'은 '산조701호'에 비해 갓 두께, 갓 직경, 대 굵기가 크고, 갓 명도와 적색도가 높았다. 3주기 수량성 조사결과 유효 개체수는 '화담'이 4.1개로 '산조701호'의 6.2개에 비해 2.1개 적었으나 개체중은 '화담'이 44.3 g으로 '산조701호'의 21.9 g 보다 높아 3주기 수량성에서 '화담'이 553.8 g으로 '산조701호'의 405 g 보다 높은 수량을 보였다. 또한, 화담은 베타글루칸이 79.4 mg/g, 페놀성 화합물 햠량은 0.93 mg GAEs/g각각 함유되어 대조품종인 '산조701호'와 유사하였고, 외래품종인 '엘808' '추재2호'에 비해 높은 함량을 보였다.

• 한국안전학회지
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• 제28권1호
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• pp.117-124
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• 2013

Emergency operating procedures(EOPs) of nuclear power plants should be described considering the cognitive capability and limitation of operators and provide appropriate information in the aspect of human factors. Procedures which doesn't consider cognitive characteristics of operators can become causes of human errors. In previous researches, in order to reduce these problems related to the description of EOP, an improvement suggestion for EOP writer's guide has been proposed, which is reflected human factors aspects that should be considered when describing EOPs. The proposed items, however, have a necessity to be validated because it was listed from various documents such as standards and guidelines without any special validation process. For that reason, in this study, a validation process were performed to show that procedures, which are described in compliance with the requirement items proposed in the improved EOP writer's guide, have positive effects in the aspects of human errors and performance comparing with previous procedures. Experiments were performed to compare the performances of two tasks which are described in compliance with each writer's guide of before and after the improvement During each task was performed in experiments, changes of physiological responses such as EEG and ECG were measured to evaluate the cognitive workload and the stress of operators in each task. And also, as the performance, frequencies of errors and cognition speeds of each task were evaluated. In the results of the experiment, the portions of the ${\beta}$ wave decreased in the tasks overall after the improvement. In the case of ECG, change rates of the mean of R-R interval were decreased in the tasks after improvement. In the results of the performance, the cognition and the response time of the tasks after the improvement were predominant with statistical significancies. Error times in the tasks after improvement were decreased or same to the tasks before improvement. Conclusively, it was validated that the procedures were described in compliance with the improved EOP writer's guide had effects on the reduction of human errors and improvement of performance.

• Journal of Pharmaceutical Investigation
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• 제29권3호
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• pp.241-245
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• 1999

Bioequivalence study of two cefpodoxime preparations, the test drug ($Banan^{\circledR}$: Hanil Pharmaceutical Co., Ltd.) and the reference drug ($Podox^{\circledR}$: Chong Kun Dang Pharmaceutical Co., Ltd.), was conducted according to the guidelines of Korea Food and Drug Administration (KFDA). Sixteen healthy male volunteers, $23.8{\pm}2.13$ years old and $63.34{\pm}4.84kg$ of body weight in average, were divided randomly into two groups and administered the drug orally at the dose of 200 mg as cefpodoxime proxetil in a $2{\times}2$ crossover study. Plasma concentrations of cefpodoxime were analysed by HPLC method for 12 hr after administration. The $AUC_{0-12hr}$ was calculated by the linear trapezoidal rule method. The $C_{max}$, and $T_{max}$ were compiled directly from the plasma drug concentration-time data. Student's t-test indicated no significant differences between the formulations in these parameters. Analysis of variance (ANOVA) revealed that there were no differences in AUC, $C_{max}$, and $T_{max}$ between the formulations. The apparent differences between the formulations were far less than 20% (e.g., 4.31, 1.99 and 4.30% for AUC, $C_{max}$, and $T_{max}$, respectively). Minimum detectable differences (%) between the formulations at ${\alpha}=\;0.05$ and $1-{\beta}=\;0.8$ were less than 20% (e.g., 13.89, 13.88, and 16.97% for AUC, $C_{max}$, and $T_{max}$, respectively). The 90% confidence intervals for these parameters were also within ${\times}20%$ (e.g., $-5,58{\sim}14.20$, $-7.89{\sim}11.88$, and $-7.78{\sim}16.38%$ for AUC, $C_{max}$, and $T_{max}$, respectively). These results satisfied the bioequivalence criteria of KFDA guidelines, indicating that the two formulations of cefpodoxime were bioequivalent.

• 한국콘텐츠학회논문지
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• 제21권11호
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• pp.320-332
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• 2021

본 연구는 국내 주식시장에서 개별종목의 주가급락위험을 과거 1년간 일별수익률의 VaR(Value-at-Risk) 통계량으로 정의하고, 주가급락위험이 기대수익률에 미치는 영향을 분석하였다. 결과는 다음과 같이 요약된다. 첫째, 전체 종목을 전월의 주가급락위험의 크기 순으로 10개의 포트폴리오로 나눈 후, 주가급락위험이 가장 높은 포트폴리오를 매수하고 가장 낮은 포트폴리오를 공매도하여 매월 구성한 무비용 포트폴리오는 월평균 -2.29%의 수익률(주가급락위험 프리미엄)을 나타낸다. 둘째, Fama-MacBeth 횡단면 회귀분석에서 기업규모, 장부가대시장가비율, 시장베타, 유동성, 최대수익률, 고유변동성, 왜도 등의 다양한 기업특성변수를 통제한 후에도 전월의 주가급락위험은 금월 수익률에 대해 유의한 음(-)의 설명력을 갖는다. 셋째, 최근 1개월 이내에 주가급락폭이 큰 종목일수록 다음 달 수익률이 더 낮다. 넷째, 전월 시장수익률의 변동성과 주가급락위험 프리미엄의 크기는 음(-)의 상관관계를 갖는다. 이러한 결과는 주가급락위험에 대해 투자자들이 과소반응하는 경향으로 인해 주가급락위험이 높은 종목일수록 주가가 고평가된다는 행태재무학적 관점에서의 가설을 지지한다.

• Journal of Microbiology and Biotechnology
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• 제29권2호
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• pp.330-338
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• 2019

Chronic infection with intracellular Brucella abortus (B. abortus) in livestock remains as a major problem worldwide. Thus, the search for an ideal vaccine is still ongoing. In this study, we evaluated the protective efficacy of a combination of B. abortus recombinant proteins; superoxide dismutase (rSodC), riboflavin synthase subunit beta (rRibH), nucleoside diphosphate kinase (rNdk), 50S ribosomal protein (rL7/L12) and malate dehydrogenase (rMDH), cloned and expressed into a pMal vector system and $DH5{\alpha}$, respectively, and further purified and applied intraperitoneally into BALB/c mice. After first immunization and two boosters, mice were infected intraperitoneally (IP) with $5{\times}10^4CFU$ of virulent B. abortus 544. Spleens were harvested and bacterial loads were evaluated at two weeks post-infection. Results revealed that this combination showed significant reduction in bacterial colonization in the spleen with a log protection unit of 1.31, which is comparable to the average protection conferred by the widely used live attenuated vaccine RB51. Cytokine analysis exhibited enhancement of cell-mediated immune response as IFN-${\gamma}$ is significantly elevated while IL-10, which is considered beneficial to the pathogen's survival, was reduced compared to control group. Furthermore, both titers of IgG1 and IgG2a were significantly elevated at three and four-week time points from first immunization. In summary, our in vivo data revealed that vaccination with a combination of five different proteins conferred a heightened host response to Brucella infection through cell-mediated immunity which is desirable in the control of intracellular pathogens. Thus, this combination might be considered for further improvement as a potential candidate vaccine against Brucella infection.

• 한국육종학회지
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• 제50권4호
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• pp.504-509
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• 2018

'흑수정찰'은 도복에 강한 흑색 찰성 보리로 육성된 품종으로 파성은 III이며 이삭의 밀도는 밀수형이고, 까락이 긴 장망형으로 출수기는 4월 29일로 조숙종인 서둔찰보리보다 3일, 성숙기는 6월 3일로 3일 정도 늦었다. 간장은 89 cm로 서둔찰보리(82 cm) 대비 7 cm 크고, 수장은 5.0 cm로 서둔찰보리(4.4 cm)보다 컷다. $m^2$ 당 수수는 543개로 서둔찰보리보다 적었다. 1수립수 54립, 천립중(36.0 g)은 무거웠다. 리터중(622 g)은 서둔찰보리보다 가벼웠다. 토양 전염 바이러스병인 보리호위축병은 저항성이었으며, 한해는 서둔찰보리보다 약하였으나 도복은 강하였다. 품질특성 중 베타글루칸 함량(6.7%)은 서둔찰보리에 비해 높았으며 아밀로스 함량(5.5%)은 낮았다. 흑색 찰성 보리로서 백도는 낮았으나 흡수율과 퍼짐성은 비슷하였다. 수량성은 전작 재배지역에서 높았으나 전체지역 평균의 수량성은 4.33 MT/ha으로 서둔찰보리와 비슷하였다.

• 대한본초학회지
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• 제33권2호
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• pp.19-28
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• 2018

Objectives : Epimedii Herba has been frequently used in Korean Traditional Medicine to treat impotence, spermatorrhoea, exophthalmos, and forgetfulness. Present study investigated anti-inflammatory effects of Epimedii Herba water extract (EWE) and attempted to elucidate molecular mechanisms involved. Methods : To explore anti-inflammatory effects of EWE, RAW 264.7 cells, a murine macrophage cell line, were pretreated with $10-100{\mu}g/m{\ell}$ of EWE, and then subsequently exposed to $1{\mu}g/m{\ell}$ of lipopolysaccharide (LPS). Levels of nitric oxide (NO), interleukin-6, $interleukin-1{\beta}$, and tumor necrosis $factor-{\alpha}$ were monitored in the medium. Expression levels of inducible nitric oxide synthase and cyclooxygenase-2 were determined by immunoblot and real-time PCR analyses. Signaling pathways related with nuclear $factor-{\kappa}B$ ($NF-{\kappa}B$) and mitogen-activated protein kinases were monitored to elucidate molecular mechanisms involved. Finally, the role of three flavonoid compounds in EWE on LPS-mediated NO production were investigated. Results : In conditioned medium, pretreatment of EWE ($100{\mu}g/m{\ell}$) significantly inhibited LPS-stimulated NO and pro-inflammatory cytokine production. In addition, EWE attenuated the expressions of inducible nitric oxide synthase and cyclooxygenase-2 by LPS. EWE prevented the phosphorylation and degradation of inhibitory ${\kappa}B{\alpha}$, nuclear translocation of $NF-{\kappa}B$, and DNA binding of $NF-{\kappa}B$, while EWE did not change the phosphorylation of mitogen-activated protein kinases by LPS. Moreover, icariin, icaritin, and quercetin partly, but significantly, inhibited the LPS-stimulated NO production. Conclusions : These results suggest that EWE has an ability to prevent inflammation in macrophages through inhibition of $NF-{\kappa}B$ signaling pathway.

• 한국식품저장유통학회지
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• 제24권8호
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• pp.1149-1157
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• 2017

본 연구에서는 넓패 에탄올 추출물의 항염증 효과를 알아보기 위해 RAW 264.7 세포에 LPS로 유도된 염증 반응 in vitro 및 in vivo 실험을 실시하였다. RAW 264.7 세포에 LPS와 함께 세포를 배양하였다. 먼저 MTT assay 실험을 통해 넓패 에탄올 추출물이 모든 농도(0.1, 1, 10, 50, $100{\mu}g/mL$)에서 세포독성을 나타내지 않는 것을 학인 하였다. 또한 모든 농도에서 염증억제 효과를 살펴 본 결과, LPS 처리구는 iNOS, COX-2, NF-${\kappa}B$ 그리고 MAPKs의 발현양을 증가 시켰으나, 넓패 에탄올 추출물의 경우 염증 반응에 관여하는 전사인자인 NF-${\kappa}B$와 MAPKs의 활성을 억제함으로써 항염증 효과를 나타내었다. 마지막으로 넓패 에탄올 추출물의 mast cell의 피부 조직학적 변화를 알아본 결과, control의 경우 진피와 경피의 면적이 확장 되어있고, mast cell의 침윤이 정상 군에 비하여 현저하게 증가함을 알 수 있었다. 반면에 넓패 에탄올 추출물의 경우 대조군에 비해 진피와 경피의 두께가 줄었으며 mast cell의 침윤감소에 효과가 있는 것을 확인하였다. 따라서 모든 결과를 종합하였을 때 넓패 에탄올 추출물이 항염증 치료제 뿐만 아니라 더 나아가 항염증에 유용한 기능성 식품소재로써 가치가 높다고 사료된다.