• Bulletin of the Korean Chemical Society
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• v.29 no.10
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• pp.1937-1940
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• 2008

The P1 duplex of Tetrahymena group I ribozyme is the important system for studying the conformational changes in folding of ribozyme. The formation of the P1 duplex between IGS and substrate RNA and the catalytic activity of ribozyme require a variety of metal ions such as $Mg^{2+}$ and $Mn^{2+}$. In order to investigate the effect of the $Mg^{2+}$ concentration on the conformation of the P1 duplex, the NMR study was performed as a function of $Mg^{2+}$ concentration. This study revealed that the less stable AU-rich region formed duplex at $50{^{\circ}C}$ under high $Mg^{2+}$ concentration condition but melts out under low $Mg^{2+}$ concentration condition. It was also found that in the active conformation under 10 mM $MgCl_2$ condition, the unstable central G${\cdot}$U wobble pair maintains the significant base pairing up to $50{^{\circ}C}$. This study provides the information of the unique feature of the P1 duplex structure and the roll of $Mg^{2+}$ ion on the formation of the active conformation.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.1A
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• pp.24-35
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• 2008

We propose a tree-based integrated network of infrastructure network and mobile ad hoc network to effectively support Mobile IP for mobile ad hoc networks and also proposed a network management protocol for formation and management of the integrated network and a tree-based routing protocol suitable for the integrated network. The integrated network has fixed gateways(IGs) that connect two hybrid networks and the mobile nodes in the network form a small sized trees based on the mobile nodes that are in the communication distance with a IG. A new node joins an arbitrary tree and is registered with its HA and FA along tree path. In addition, the proposed protocol establishes a route efficiently by using the tree information managed in every node. We examined the effectiveness of the tree-based integrated network for some possible network deployment scenarios and compared our routing protocol against the Mobile IP supported AODV protocol.