• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.15 no.5
• /
• pp.3159-3168
• /
• 2014

Interevent Time Definition(IETD) is essential in CSOs(Combined Sewer Overflows) tank design for the treatment of non-point source pollution. However, existent rainfall analysis can not calculate total amout of non-point source pollution. For deviding continuous rainfall events, therre are several IETD calculating methods. In this research, After calculate IETD by existents methods, problem of results is identified as existents method. To supplement these problems, new method using exponential decay function is suggested. The confidence range of ${\lambda}$ is estimated.

• Journal of Life Science
• /
• v.25 no.4
• /
• pp.385-389
• /
• 2015

In this study, it is reported that a large polyprotein can be stoichiometrically cleaved by the use of caspase-3-dependent proteolysis. Previously, it has been shown that the proteolytic IETD motif was partially processed when treated with caspase-3, while the DEVD motif was completely cleaved. The cleavage efficiency of the DEVD-based substrate was approximately 2.0 times higher than that of the IETD substrate, in response to caspase-3. Based on this, 3 protein genes of interest were genetically linked to each other by adding two proteolytic cleavage sequences, DEVD and IETD, for caspase-3. Particularly, glutathione-S transferase (GST), maltose binding protein (MBP), and red fluorescent protein (RFP) were chosen as model proteins due to the variation in their size. The expressed polyprotein was purified by immobilized metal ion affinity chromatography (IMAC) via a hexa-histidine tag at the C-terminal end, showing 93 kDa of a chimeric GST:MBP:RFP fusion protein. In response to caspase-3, cleavage products, such as MBP:RFP (68 kDa), MBP (42 kDa), RFP (26 kDa), and GST (25 kDa), were separated from a large precursor GST:MBP:RFP (93 kDa) via SDS-PAGE. The results obtained from this study indicate that a multi-protein can be stoichiometrically produced from a large poly-protein by using proteolytic recognition motifs, such as DEVD and IETD tetra-peptides, for caspase-3.

• Journal of Microbiology and Biotechnology
• /
• v.19 no.9
• /
• pp.911-917
• /
• 2009

The apoptotic caspases have been classified in accordance with their substrate specificities, as the optimal tetrapeptide recognition motifs for a variety of caspases have been determined via positional scanning substrate combinatorial library technology. Here, we focused on two proteolytic recognition motifs, DEVD and IETD, owing to their extensive use in cell death assay. Although DEVE and IETD have been generally considered to be selective for caspase-3 and -8, respectively, the proteolytic cleavage of these substrates does not display absolute specificity for a particular caspase. Thus, we attempted to monitor the cleavage preference for caspase-3, particularly using the recombinant protein substrates. For this aim, the chimeric GST:DEVD:EGFP and GST:IETD:EGFP proteins were genetically constructed by linking GST and EGFP with the linkers harboring DEVD and IETD. To our best knowledge, this work constitutes the first application for the monitoring of cleavage preference employing the recombinant protein substrates that simultaneously allow for mass and fluorescence analyses. Consequently, GST:IETD:EGFP was cleaved partially in response to caspase-3, whereas GST:DEVD:EGFP was completely proteolyzed, indicating that GST:DEVD:EGFP is a better substrate than GST:IETD:EGFP for caspase-3. Collectively, using these chimeric protein substrates, we have successfully evaluated the feasibility of the recombinant protein substrate for applicability to the monitoring of cleavage preference for caspase-3.

• Journal of Korea Water Resources Association
• /
• v.40 no.8
• /
• pp.655-663
• /
• 2007

This study suggests a new time distribution method of rainfall for small urban watersheds. IETD (Interevent Time definition) determination method considering basin characteristics and dimensionless accumulation rainfall curves involving rainfall events with shorter duration than 3-hours are suggested. A new definition of IETD is the time period from the end of a rainfall event to the end of a direct runoff. Using the method, we drive an area-IETD regression curve for the Joong-Rang basin. The rainfall event with 10 year-return periods, 2-hour duration is distributed and applied four urban watersheds. In the four watersheds, we calculate hydrographs for four watersheds using SWMM and compare them with ones of the Huff's distribution model. From the comparison, we find that peak flows resulted from the developed methodology are $11\sim15%$ larger than ones from the Huff's model. As conclusion, the Huff method should be adopted for the urban watersheds with careful verification.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2011.05a
• /
• pp.281-285
• /
• 2011

본 연구에서는 연최대치 독립 호우사상 계열과 연최대치 계열의 차이를 살펴보았다. 이를 위해 본 연구에서는 몇 가지 경우의 IETD 및 절단값을 적용하여 독립 호우사상을 결정하고, 그 특성을 살펴보았다. 이어 연최대치 계열과 연 최대치 독립 호우사상 계열을 비교하였다. 본 연구는 1961년부터 2010년까지 서울지점의 시강우 자료를 분석대상으로 사용하였다. 그 결과, IETD의 증가에 따라서 독립 호우사상의 발생빈도 및 평균 강우강도는 감소하고, 평균 지속기간은 증가하였다. 절단값의 증가에 따라 독립 호우사상의 발생빈도 및 평균 지속기간은 감소하고, 평균 강우강도는 증가하였다. 호우사상의 평균 강우강도는 강우 지속기간에 관계없이 거의 일정한 것으로 나타났다. 이러한 결과를 통해 지속기간이 짧은 호우사상의 최대 강우강도는 지속기간이 긴 호우사상의 최대 강우강도보다 매우 작을 것으로 파악되었다. 지속 기간이 짧은 경우, 연 최대치 계열과 연 최대치 독립 호우사상 계열의 차이는 매우 크며, 강우 지속기간이 길게 적용한 경우에는, 두 계열의 차이는 매우 줄어드는 것으로 나타났다.

• Journal of Korea Water Resources Association
• /
• v.55 no.8
• /
• pp.603-611
• /
• 2022

In urban areas, the impermeable area continues to increase due to urbanization, which interferes with the surface penetrating and infiltrating of rainwater, causing most rainwater runoff to the surface, deepening the distortion of water circulation. Distortion of water circulation affects not only flood disasters caused by rainfall and runoff, but also various aspects such as dry stream phenomenon, deterioration of water quality, and destruction of ecosystem balance, and the Ministry of Environment strongly recommends the use of Low Impact development (LID) techniques. In order to apply the LID technique, it is necessary to set a rainwater management target to handle the increase in outflow after the development of the target site, and the current standard sets the rainwater management target using the 10-year daily rainfall. In this study, the difference from the current standards was analyzed through statistical analysis and classification of independent rainfall ideas using inter-event time definition (IETD) in setting the target amount of rainwater management to improve water circulation. Using 30-year rainfall data from 1991 to 2020, methods such as autocorrelation coefficient (AC) analysis, variation coefficient (VC) analysis, and annual average number of rainfall event (NRE) analysis were applied, and IETD was selected according to the target rainfall period. The more samples the population had, the more IETD tended to increase. In addition, by analyzing the duration and time distribution of independent rainfall according to the IETD, a plan was proposed to calculate the standard design rainfall according to the rainwater management target amount. Therefore, it is expected that it will be possible to set an improved rainwater management target amount if sufficient samples of independent rainfall ideas are used through the selection of IETD as in this study.

• Journal of Korea Water Resources Association
• /
• v.45 no.5
• /
• pp.431-444
• /
• 2012

This study investigated the differences between annual maximum series and annual maximum independent rainfall event series with relatively short and long rainfall durations. Annual maximum independent rainfall events were selected by applying various IETDs and thresholds to the hourly rainfall data in Seoul for the duration from 1961 to 2010. Annual maximum independent rainfall event series decided were then compared with the conventional annual maximum series. Summarizing the results is as follows. First, the effect of IETD and threshold was not beyond the expected level. For example, as the IETD increases, the frequencies of independent rainfall events decreased similarly in their rate for both with short and long durations. However, as the threshold increases, the frequency of those with rather long durations decreased much higher. Second, The mean rainfall intensity of the independent rainfall events was found to remain constant regardless of their duration. This indicates that the annual maximum rainfall intensity could be found in a rainfall event with longer durations. Lastly, the difference between the annual maximum rainfall series and the annual maximum independent rainfall event series with rather short rainfall durations was found significantly large, which decreases with longer durations. This result indicates that the conventional data analysis method, especially for small basins with short concentration time, could lead an unrealistic design rainfall with little possibility of occurrence.

• Journal of the Korean Geotechnical Society
• /
• v.32 no.4
• /
• pp.15-27
• /
• 2016

This study was conducted to suggest a landslide triggering rainfall threshold (ID curve) for landslide prediction by considering the effect of antecedent rainfall. 202 rainfall data including domestic landslide and rainfall records were used in this study. In order to consider the effect of antecedent rainfall, rainfall data were analyzed by changing Inter Event Time Definition (IETD) and IETD based ID curve were presented by regression analysis. Compared to the findings of the previous studies, the presented ID curve has a tendency to predict the landslides occurring at a relatively low rainfall intensity. It is shown that the proposed ID curve is appropriate and realistic for predicting landslides through the validation of proposed ID curve using records of landslides in 2014. Based on this analysis, it is found that the longer IETD, the greater the effect of antecedent rainfall, and the steeper the gradient of ID curve. It is also found that the rainfall threshold (intensity) is higher for the short period rainfall and lower for the long period rainfall.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.59 no.6
• /
• pp.73-80
• /
• 2017

The objective of this study is to investigate whether the daily rainfall depth derived from daily data represents the event rainfall depth derived from hourly data. For analysis, the 85th, 90th, and 95th percentile daily rainfall depths were first computed using daily rainfall data (1986~2015) collected at 63 weather stations. In addition, the storm event was separated by the interevent time definition (IETD) of 6, 12, 18, and 24 hr using hourly rainfall data. Based on the separated storm events, the 85th, 90th, and 95th percentile event rainfall depths were calculated and compared with the using hourly rainfall data with the 85th, 90th, and 95th percentile daily rainfall depths. The event rainfall depths computed using the IETD were greater than the daily rainfall depths. The difference between the event rainfall depth and the daily rainfall depth affects the design and size of the facility for controlling the stormwater. Therefore, the designer and policy decision-maker in designing the stormwater best management practices need to take into account the difference generated by the difference of the used rainfall data and the selected IETD.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2021.06a
• /
• pp.298-298
• /
• 2021

본 연구에서는 국내 6개 지역 서울, 강릉, 대전, 광주, 부산, 제주의 30년 치 시강우 자료에 해석적 확률모형(Analytical Probabilistic Models) 방법을 적용하여 도시 홍수 저감을 목적으로 하는 저류시설 설계를 위한 유출량 예측 정도를 지역별로 비교하고자 하였다. 강우 사상 분포의 해석적 확률모형을 적용하기 위해 무강우 시간을 결정하여 독립 호우를 결정하는데, 자기상관계수와 변동계수를 활용한 무강우 지속시간의 산정(IETD, Interevent Time Definition) 방법을 사용하였다. 해석적 확률모형인 유출량의 확률밀도함수(PDF, Probability Density Function)를 유도하기 위해서 불투수 지역과 투수 지역의 영향을 고려하여 유출계수를 적용하는 강우-유출 관계를 가지고 유출량을 정의하였다. 강우량, 강우 지속시간, 무강우시간과 같은 강우특성은 1변수 지수함수의 PDF를 따른다고 가정하였다. 확률모형 방법의 적합성을 판단하기 위해 결정된 IETD에 따라 각 지역별로 실제 강우 사상을 해석적 모델과 연속모의실험인 SWWM(Storm Water Management Model)에 적용하여 불투수율에 따른 유출량을 산정하였다. 각 방식으로 얻은 유출량 결과는 모든 지역에서 매우 유사하게 나타났고 결론적으로 우리나라에서 도시 홍수 저감을 위한 저류시설의 계획과 설계에 확률모형 방법이 적용 가능하다는 것을 확인할 수 있었다.