• Title/Summary/Keyword: IE

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Case Study on the Impact-Echo Method for Tunnel Safety Diagnosis (터널 안전진단을 위한 충격반향법 사례 연구)

  • Shin, Sung-Ryul;Jo, Cheol-Hyun
    • Tunnel and Underground Space
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    • v.19 no.1
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    • pp.19-30
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    • 2009

  • For the purpose of determining the thickness of concrete lining and detect of the cavity where may be located behind tunnel lining, IE (Impact-Echo) method it effectively useful in the tunnel safety diagnosis and the quality control during the construction. As a part of case study, we applied IE method to various tunnel structure types such as road tunnel and subway tunnel constructed by NATM (New Austrian Tunnelling Method) and ASSM (American Steel Support Method). As tunnel specifications estimated from this method were compared with coring data, design drawing and other survey results, it was very good agreement with each other. In conclusion, we verified that IE method shows an accurate and reliable result. The conventional interpretation of IE method in frequency domain gives only vertical information at a certain point. However, the interpretation using time-frequency analysis and depth section imaging technique from two dimensional profiling surveys can show more reliable information about structure inside.

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Insect Cell Surface Expression of Hemagglutinin (HA) of Egyptian H5N1 Avian Influenza Virus Under Transcriptional Control of Whispovirus Immediate Early-1 Promoter

  • Gadalla, M.R.;El-Deeb, A.H.;Emara, M.M.;Hussein, H.A.
    • Journal of Microbiology and Biotechnology
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    • v.24 no.12
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    • pp.1719-1727
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    • 2014

  • In the present study, whispovirus immediate early 1 promoter (ie-1) was used to initiate surface expression of the hemagglutinin (HA) protein of Egyptian H5N1 avian influenza virus (AIV) by using the baculovirus expression vector system. The HA gene and whispovirus ie-1 promoter sequence were synthesized as a fused expression cassette (ie1-HA) and successfully cloned into the pFastBac-1 transfer vector. The recombinant vector was transformed into DH10Bac competent cells, and the recombinant bacmid was generated via site-specific transposition. The recombinant bacmid was used for transfection of Spodoptera frugiperda (Sf-9) insect cells to construct the recombinant baculovirus and to induce expression of the HA protein of H5N1 AIV. The recombinant glycoprotein expressed in Sf-9 cells showed hemadsorption activity. Hemagglutination activity was also detected in both extra- and intracellular recombinant HAs. Both the HA and hemadsorption activities were inhibited by reference polyclonal anti-H5 sera. Significant expression of the recombinant protein was observed on the surface of infected insect cells by using immunofluorescence. SDS-PAGE analysis of the expressed protein revealed the presence of a visually distinguishable band of ~63 kDa in size, which was absent in the non-infected cell control. Western blot analysis confirmed that the distinct 63 kDa band corresponded to the recombinant HA glycoprotein of H5N1 AIV. This study reports the successful expression of the HA protein of H5N1 AIV. The expressed protein was displayed on the plasma membrane of infected insect cells under the control of whispovirus ie-1 promoter by using the baculovirus expression vector system.

  • https://doi.org/10.4014/jmb.1405.05028 인용 PDF KSCI KPUBS HTML

Analysis of Irrigation Efficiency and Pattern in Galshin Pumping District (갈신양수장 관개지구의 관개효율과 관개패턴분석)

  • Ryu, Bumhee;Park, Seungki
    • Journal of Korean Society of Rural Planning
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    • v.26 no.3
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    • pp.91-102
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    • 2020

  • The purpose of study was analyze the pumping characteristics, Irrigation Efficiency(IE), and irrigation pattern by period of rice growing stage with based on the performance of design irrigation water requirement and operational Galshin Pumping(GP) station in GP irrigation district constructed under rural water development project master plan. GP station was located in Yedang reservoir, Yesan-gun, Chungcheongnam-do and has been supplying irrigation water since 2006. The research data are the Irrigation Water Requirement(IWR) and the Pumping Water Amount(PWA) from 2006 to 2015 at the GP station, which is the supplied amount. The IWR were calculated using the Blaney-Criddle formula of the HOMWRS program, Hydrological Operation Model for Water Resource System, developed by Korea Rural Community Corporation. The Blaney-Criddle formula was used to calculate design irrigation water requirement of Galshin rural water development project master plan. During 2006-2015, the study period, the annual average IWR is 763.2(±149.1)mm, the annual PWA of the GP station is 397.4mm to 1,056.9mm, and those average annual PWA is 643.4(±208.4)mm. The annual IE of GP station 96.5% to 169.0%, and the average annual IE is 124.3%, which is higher than the research results conducted in other pumping stations. Analyzing the irrigation patterns of the GP irrigation district, the IWR Ratio per 10days(IWRR) and the PWA Ratio per 10days(PWAR) of the G P station were obtained. The IWRR is the percentage of IWR for each 10 days of a month to total IWR per year, and the PWAR is the percentage of PWA for each 10 days of a month to total PWA per year. The Kolmogorov- Smirnov(K-S) test results of IWRR and PWAR showed the characteristics classification by rice growing stage and stable normal distribution characteristics. Average IWRR(AIWRR) and Average PWAR(APWAR) are presented as irrigation patterns. Irrigation pattern analysis will be able to standardize comparison, analysis and probability calculation of the pumping station characteristics of different pumping stations and apply to objective evaluation of the pumping station district.

  • https://doi.org/10.7851/Ksrp.2020.26.3.091 인용 PDF KSCI