• Journal of IKEEE
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• v.23 no.2
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• pp.614-621
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• 2019

In this paper, we presents the integral equation-fast Fourier transform(IE-FFT) and block matrix preconditioner (BMP) to solve electromagnetic scattering problems of penetrable structures composed of dielectric or magnetic materials. IE-FFT can significantly improve the amount of calculation to solve the matrix equation constructed from the moment method(MoM). Moreover, the iterative method in conjunction with BMP can be significantly reduce the number of iterations required to solve the matrix equations which are constructed from electrically large structures. Numerical results show that IE-FFT and block matrix preconditioner can solve electromagnetic scattering problems for penetrable objects quickly and accurately.

• BMB Reports
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• v.35 no.6
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• pp.553-561
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• 2002

A gene that encodes a protein homologue to baculoviral IE-1 was identified and sequenced in the genome of the Choristoneura fumiferana granulovirus (ChfuGV). The gene has an 1278 nucleotide (nt) open-reading frame (ORF) that encodes 426 amino acids with an estimated molecular weight of 50.33 kDa. At the nucleotide level, several cis-acting regulatory elements were detected within the promoter region of the ie-1 gene of ChfuGV along with other studied granuloviruses (GVs). Two putative CCAAT elements were detected within the noncoding leader region of this gene; one was located on the opposite strand at -92 and the other at -420 nt from the putative start triplet. Two baculoviral late promoter motifs (TAAG) were also detected within the promoter region of the ie-1 gene of ChfuGV. A single polyadenylation signal, AATAAA, was located 18nt downstream of the putative translational stop codon of ie-1 from ChfuGV. At the protein level, the amino acid sequence data that was derived from the nucleotide sequence in ChfuGV IE-1 was compared to those of the Cydia pomonella granulovirus (CpGV), Xestia c-nigrum granulovirus (XcGV) and Plutella xylostella granulovirus (PxGV). The C-terminal regions of the granuloviral IE-1 sequences appeared to be more conserved when compared to the N-terminal regions. A domain, similar to the basic helix-loop-helix like (bHLH-like) domain in NPVs, was detected at the C-terminal region of IE-1 from ChfuGV (residues 387 to 414). A phylogenetic tree for baculoviral IE-1 was constructed using a maximum parsimony analysis. A phylogenetic estimation demonstrates that ChfuGV IE-1 is most closely related to that of CpGV.

• Korean Journal of Veterinary Service
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• v.27 no.4
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• pp.355-369
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• 2004

The equine herpesvirus type 1 (EHV-1) immediate-early (IE) protein is a potent transactivator responsible for the activation of both early and late genes during the course of infection and is comprised of discrete functional domains that mediate its many functions. Interaction between trans activators such as the IE protein and various components of the RNA polymerase II transcription initiation machinery has been demonstrated to be critical for transactivation. In the present report, it is addressed the hypothesis that the IE protein interacts with various components of transcription machinery to mediate transactivation of target viral genes. In these studies, it is demonstrated that in vitro transcribed and translated IE protein interacts with TFIIB-agarose conjugate but not with TFIID-agarose conjugate. Additional immunoprecipitation studies using nuclear extracts derived from EHV-1 infected RK-13 cells confirmed that the IE protein interacts strongly with TFIIB, but fails to interact with TFIID. IR2, a truncated form of the IE protein lacking the potent transactivation domain and involved in the down-regulation of the IE gene, also interacted with TFIIB but not with TFIID. Studies were also performed to ascertain if particular TBP-associated factors (TAFs) could mediate IE or IR2 binding to TFIID. In vitro transcribed and translated TAF250 added to nuclear extracts generated from EHV-1 infected cells also failed to mediate an interaction between the IE protein or the IR2 protein and TFIID. This study demonstrated that the IE protein mediates transactivation of target viral genes by a mechanism that involves TFIIB. This is in contrast to mechanisms that have been proposed for both the herpes simplex virus ICP4 and VP16 protein which have been proposed to transactivate viral genes through interactions involving both TFIIB and TFIID. This study also intimates that IR2 mediate its repressive effects during the course of EHV-1 infection by a mechanism that involves sequestration of various transcription factors.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.12
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• pp.372-383
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• 2017

This paper attempted to verify that Investigative Enterprising(IE) axis in the Holland hexagonal model can measure the internal and external job value. This study analyzed internal and external job values of 19 subjects who participated in the 150 Job cards classification test. The results of this study are as follows: First, the study group with Holland hexagonal model centered on the Investigative Enterprising type(IE) axis and artistic type(A) and social type(S) showed internal job value and supported the hypothesis. Second, the hypothesis that the group with the hexagonal model centered on the Investigative Enterprising(IE) axis and the bias toward the realistic type(R) and the conventional type(C) would pursue external job value was rejected. This is due to the Korean cultural context that pursues psycho-cultural value in Confucian culture. There is also a Holland hexagonal model that is not exactly distributed to the left of the Investigative Enterprising(IE) axis. Third, the group of amphibolic job value based on the Investigative Enterprising(IE) axis, and the Holland hexagonal model is expressed in artisic type(A), social type(S), realistic type(R), and conventional type(C) supported some hypotheses. This paper is the first to suggest that the Investigative Enterprising(IE) axis of the Holland hexagonal model can be used to measure job value, and the Holland hexagonal model can predict job value as well as career choice. This paper is intended to expand the foundation of the Holland theories, and to provide meaningful contribution to the basis for vocational studies.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.8 no.3
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• pp.147-155
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• 2013

This paper study is for enhance the productivity. The purpose of this study is save the cost and contribute the enhancing company's competitiveness by analysis overall wastes in production line and improve the wastes in production line. Many company does not have clear process for analysis the wastes in production line. so they use classical IE tool and advanced IE tool by considering their current situation but the result was not so effective. The study to arrange the classic IE and advanced IE by each step to analysis the wastes by each production line include assembly line and processing line and automation line so that we can predict the mount of the overall wastes and easily select / focus on the tool for maximize the improvement result. It will contribute to establish the mid term and long term improvement activity plan.

• Journal of the Korean earth science society
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• v.32 no.1
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• pp.1-11
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• 2011

The impact echo and impulse response methods were applied to the safety inspection of concrete structure, which has the rear cavity. The concrete structure model used in this study was divided into four sections, pure concrete, concrete+cavity, reinforced concrete with iron bar, and reinforced concrete+cavity, respectively. Previous study performed by authors have showed a possibility of success to use these method for detection of the rear cavity of concrete structure. Therefore, we tried to get more enhanced result with IE and IR methods through this study. Especially, IE and IR methods are relatively accurate to map the point of measurement, which makes it possible to interpret the depth of the concrete bed and effect by rear cavity with confidence. Followings were revealed from the results; the IE method shows some small peak zones probably indicating the rear cavity in the frequency lower than the resonance frequency and the changes of mobility and dynamic stiffness in the IR method indicate the weak zones. The proposed methods can be used to delineate the weak zones of the concrete structure.

• Korean Journal of Veterinary Service
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• v.25 no.4
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• pp.333-346
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• 2002

We previously reported that the equine herpesvirus type 1(EHV-1) immediate-early protein(IE protein) physically interacts with the general transcription factor human TFIIB(Jang et al, J Virol 75：10219-10230, 2001). The interaction between the IE protein and TFIIB is necessary for the IE protein to efficiently transactivate the early TK and late IR5 EHV-1 promoters. A panel of deletion and truncation mutants of the TFIIB gene was constructed and employed in protein-binding assays to map the IE protein-binding domain within TFIIB. Evidence is presented that the first direct repeat of TFIIB interacts specifically with the EHV-1 IE protein.

• Journal of Sericultural and Entomological Science
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• v.40 no.2
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• pp.111-116
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• 1998

To construct transformed Bm5 cells, Autographa californica nuclear polyhedrosis virus (AcNPV)IE1 gene, an immediate early viral gene was firstly used in this study. AcNPV IE1 gene, which shares on 95.3% uncleotide sequence homology with Bombyx mori nuclear polyhedrosis virus (BmNPV) IE1 gene, was isolated and cloned into pBluescript. Neomycin gene from pKO-neo was inserted under the control of the IE1 promoter to yield pAcIE1-neo. The plasmid pAcIE1-neo was transfected into Bm5 or Sf9 cells, and neomycin-resistant cells were selected in TC100 medium containing 10% fetal bovine serum (FBS) and 1 mg/$m\ell$ G418 for two weeks. Individual clones were picked and each was amplified for further characterization. The genomic DNA from neomycin-resistnt cells was isolated and characterized by PCR using AcNPV IE1 gene-specific primers and by Southern blot analysis using neomycin gene probe. We concluded that AcNPV IE1 gene was functional in B. moridrived Bm5 cells as well as Spodaptera frugiperda-derived Sf9 cells to produce stably-transformed insect cells.

• Restorative Dentistry and Endodontics
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• v.46 no.1
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• pp.7.1-7.8
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• 2021

Objectives: The aim of the current study is to investigate the effect of different anticaries agents, such as experimental agents based on silver nanoparticles (SNPs) and silver diamine fluoride (SDF), on the micro-shear bond strength (μ-SBS) of composite resin applied to intact enamel (IE) or demineralized enamel (DE). Materials and Methods: Sixty dental enamel fragments were collected from human third molars and categorized into 6 groups (n = 10): positive control (IE), negative control (DE), IE + SDF, DE + SDF, IE + SNP and DE + SNP. Samples from DE, DE + SDF and DE + SNP groups were subjected to pH cycling; superficial microhardness test was performed to confirm demineralization. Resin composite build-ups were applied to the samples (0.75-mm diameter and 1-mm height) after the treatments (except for IE and DE groups); μ-SBS was also evaluated. Samples were analyzed under a stereomicroscope at 40× magnification to identify failure patterns. Data were subjected to one-way analysis of variance, followed by Tukey's and Dunnett's tests (p < 0.05). Results: There was no significant difference among the IE, IE + SNP, DE + SDF, and DE + SNP groups. The IE + SDF and DE groups recorded the highest and the lowest μ-SBS values, respectively. Adhesive-type failures were the most frequent for all treatments. Conclusions: Anticaries agents did not have a negative effect on the μ-SBS of composite resin when it was used on IE or DE.

• Journal of Korea Technology Innovation Society
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• v.8 no.spc1
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• pp.525-554
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• 2005

The purpose of this paper is to seek out the problems influencing on the operation of Institution Evaluation(IE) in Korea and suggest a better way for the evaluation based on the findings, expecially focusing on Government Research Institute(GRIs) in the field of Science and Technology. Unlike previous studies on IE of GRIs, this research is more oriented toward actual practices than design and theories of IE. Three categories are 1) purely system aspect, 2) system-operation aspect, and 3) environmental aspect. These three categories are classified into eight sub-categories: 1) the legal support and the legally enforceable provision, 2) the target area of evaluation, 3) the means and process of evaluation, 4) the criteria and indices for evaluation, 5) the expertise of evaluators, 6) the use of evaluation findings, 7) GRIs' support for IE, and 8) public support for IE. Summing up the findings of this study, the main factors that hamper the successful operation of the existing IE of GRIs in the field of Science and Technology was illuminated and the better way for the operation of IE was suggested.