• 한국표면공학회지
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• 제41권6호
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• pp.279-286
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• 2008

Numerical analysis is done to investigate which would be the most influencing process parameter in determining the uniformity of deposition thickness in TiN ICP-CVD(inductively coupled plasma chemical vapor deposition). Two configurations of ICP antenna are modeled; side and top planar. Side and top gas inlets are considered with each ICP antenna geometries. Precursor for TiN deposition was TDMAT(Tetrakis Diethyl Methyl Amido Titanium). Two step volume dissociation of TDMAT is used and absorption, desorption and deposition surface reactions are included. Most influencing factors are H and N concentration dissociated by electron impact collisions in plasma volume which depends on the relative positions of gas inlet and ICP antenna generated hot plasma region. Low surface recombination of N shows hollow type concentration, but H gives a bell type distribution. Film thickness at substrate edges is sensitive to gas flow rate and at high pressures getting more dependent on flow characteristics.

• 분석과학
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• 제20권3호
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• pp.251-254
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• 2007

수용액 내에서 환원제인 sulfurous acid와 휘발성이 강한 Os(III)을 반응시킨 후 생성된 비휘발성 Os(IV)을 inductively coupled plasma atomic emission spectrometry (ICP-AES)로 분석하였다. pH 2-10의 수용액에서 sulfurous acid와 Os(VIII)의 정량적 반응에 의하여 생성된 Os(VI)을 ICP-AES로 분석하였다. 동축형 분무기(concentric type nebulizer)에 의한 시료 도입 방법에서 sulfurous acid와 osmium은 반응 직후부터 안정한 신호를 나타내었으며 다양한 ICP-AES 조건에서 3회 반복 측정했을 때 재현성은 0.5-4.5%였으며, 검출 한계는 2.5-57.7 ng/g이었다.

• 대한한방내과학회지
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• 제36권3호
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• pp.217-227
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• 2015

Objectives: The primary aim of this study was to identify bioactive compounds in Dangnyo-hwan, a Korean herbal medicine, through instrumental analysis using LC-MS/MS and ICP, and investigate its potential use in diabetes treatment. Methods: The extract of Dangnyo-hwan has 12 medicinal herbs; these were compared with 18 marker substances selected from literature survey. Results: LC-MS/MS analysis could detect 9 of the 18 bioactive compounds: citruline, catalpol, berberine, ginsenoside Rb1, ginsenoside Rg1, oleanolic acid, β-sitosterol, mangiferin, and schizandrin. While harmful heavy metals such as As, Pb, Cd, Hg, Ni, and Cu were not present in high concentrations, Zn concentration was 4.2 mg in 100 g Dangnyo-hwan. Conclusions: Instrumental analysis such as LC-MS/MS and ICP was successfully used to identify bioactive compounds in Dangnyo-hwan. Detection of 9 bioactive substances and Zn from the herb medicine is a valuable finding, and suggests that Dangnyo-hwan is a candidate medicine for diabetes. Further investigations like in vitro assay, percent GPR 119 activity, and percent human DGAT-1 inhibition are underway.

• 분석과학
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• 제20권6호
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• pp.496-501
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• 2007

ICP-AES를 이용하여 금을 분석할 때 감도가 가장 우수한 242.795 nm 파장은 분광학적 간섭을 일으키는 망간, 크롬, 코발트, 철 등의 원소들 때문에 광물에서 금의 분석은 불가능하다. 여기에서는 이들 원소들을 정량적으로 분리하는 분리방법을 연구하였다. 광물에서 금을 분리하기 위하여 광물을 용해한 산 용액에 MIBK 용매를 사용하여 정량적으로 추출하였다. 이때 질산과 염산의 혼산과 MIBK/n-hexane의 혼합용매는 효율적으로 금을 추출할 수 있었으며, 회수율은 97.5% 이상이었다.

• 한국미생물·생명공학회지
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• 제26권6호
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• pp.497-506
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• 1998

Bacillus thurintensis subsp. kurstaki HD1 살충성 단백질 ICP 유전자가 있는 NdeI 단편 3.856 kb를 클로닝하여 제조한 pHLN2-80(-) 클론이 pHLN1-80(-)에 비해서 대장균에서 ICP발현량이 과다발현되는 현상을 규명하고자 하였다. 본 연구에서는 상기의 pHLN2-80(+) 클론의 발현량을 조절하는 원인을 규명하기 위하여 ICP의 아미노산 서열은 변화되지 않는 범위 내에서 pHLN1-80(+) 클론에 있는 Plac프로모터와 ICP유전자 프로모터의 일부인 -80 bp프로모터의 염기서열, 전사 개시점과 종결부위의 변이가 ICP유전자발현에 미치는 영향을 조사하였다. pHLN1-80(+)에 5'-말단에 존재하는 -80 bp 프로모터만을 보유한 pHLNK-80 클론은 ICP 생산은 매우 저조하였다. Plac프로모터와 -80 bp 프로모터의 구조 골격을 일부 변이 시킨 pHLNF1-80클론의 ICP생산량은 pHLN2-80(-)가 생산한 양보다는 낮아서 과다발현이 안되었다. Plac프로모터 상류를 약 350bp을 제거하여 만든 클론 pHLND2-80의 ICP 생산량은 모클론인 pHLN2-80(-) 보다 매우 높게 과다발현 되었다. ICP 유전자의 과다발현 현상에 대한 전사 개시점과 전사종결 부위의 역할을 알아보기 위해서 -72bp ICP유전자프로모터를 갖는 클론 pHLD1-72는 재조합 클론 pHLN2-80(-)가 생산한 양보다 적은 양의 ICP을 생성하였고, 클론 pHLD2-72는 재조합 클론 pHLN2-80(-)보다 적은 ICP을 발현하여 과다 발현되었으며, 클론 pHLN2-72는 모클론인 pHLN2-80(-)보다 약간 높은 ICP 생산량을 보여 과다발현되었다. 클론 pHLN2-72를증식하여 파쇄액을 만든 후에 Bombyx mori유충에 대한 살충력 검사에서 클론 pHLN2-72이 생산한 ICP는 pHLN1-80(+)이 생산한 ICP보다 약 90배의 살충력을 보였다. SDS-PAGE와 Western blot 분석에서도 클론 pHLN2-72는 재조합 클론 pHLN2-80(-)보다 약간 높게 ICP가 생성이 되었었다. 이상의 결과는 과다발현에 Plac프로모터와 종결부위가 반드시 필요하며, -72 bp ICP 프로모터가 -80 bp 프로모터보다 과다발현률이 높았으며, ICP 유전자는 반드시 Plac프로모터의 전사 방향에 역방향으로 삽입이 되어야 하는 것으로 나타났다.

• Mass Spectrometry Letters
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• 제6권4호
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• pp.85-90
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• 2015

This article reviews recent analytical techniques using inductively coupled plasma-mass spectrometry (ICP-MS) immunoassay for clinical and bio analysis. We classified the techniques into two categories, direct and indirect analysis, which depend upon a guideline of whether tagging materials are used or not. Direct analysis is well known, and generally used in conjunction with various other techniques, such as laser ablation, chromatographic separations, etc. Recently, indirect analysis using tagging elements has intensively been discussed because of its importance in future applications to bio and clinical analysis, including environmental and food industries. The method has shown advantages of multiplex detection, excellent sensitivity, and short analysis time owing to signal amplification and magnetic separation. Now, it expands the application field from small biomolecules to large cells.

• 한국연초학회지
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• 제31권2호
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• pp.95-106
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• 2009

Semiquantitative analysis by ICP-MS has proven to be a powerful tool for fast screening, in addition, it does not require the element of interest to be present in the calibration standard, making it especially useful for the analysis of unknown samples. In this study, seven cigarette samples were analyzed by the rapid semi-quantitative analysis method based on the ICP-MS. For each cigarette sample, cut tobacco, cigarette paper, filter (before and after smoking), and smoke condensate were analyzed. The accuracy of the analysis technique was evaluated by comparing results obtained from Calibration Check Standard(CCS) and calibration method. Relative Percentage Error(RPE) value of all elements measured for three CCS showed a stable result of less than ${\pm}20%$. Compared to full quantitative analysis by calibration method, the results for cigarette samples showed average error within ${\pm}15%$.

• 대한간호학회지
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• 제36권2호
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• pp.353-360
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• 2006

Purpose: This study was done to investigate the effects of backrest elevation of 0 degree and 30 degrees that minimize the risk of increasing ICP when CVP is measured. Methods: Subjects were sixty-four patients who stayed in the neurosurgical intensive care unit after brain surgery at two university-based hospitals. CVP, blood pressure, heart rate and ICP were measured along with position changes in order of backrest position with primary 30 degrees backrest position, 0 degree backrest position and secondary 30 degrees backrest position. For data analysis, one-group, repeated-measures analysis of variance design was used in SAS program. Results: Backrest elevations from 0 degree to 30 degrees did not alter the CVP without increasing the ICP. Therefore, 30 degrees backrest position is a preventive position without increasing ICP. Conclusion: 30 degrees backrest position might be appropriate for brain injury patients when CVP is measured.

• 한국대기환경학회:학술대회논문집
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• 한국대기환경학회 2004년도 춘계학술대회 논문집
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• pp.139-140
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• 2004

• Bulletin of the Korean Chemical Society
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• 제17권8호
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• pp.769-771
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• 1996