• Journal of Physiology & Pathology in Korean Medicine
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• 제30권3호
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• pp.164-176
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• 2016

This study was perfomed to investigate the effects of Chunghyul-plus(CHP) on oxidative damage and hyperlipidemia in db/db mouse. After treatment with CHP, safety in cytotoxicity, heavy metal toxicity, production of reactive oxygen species(ROS), nitric oxide (N0) and proinflammatory cytokine IL-Ib, TNF-a, IL-6 in RAW 264.7 cells. Serum total cholesterol, LDL cholesterol, HDL cholesterol, triglyceride, insulin, GLP-1, glucose, food intake, body weight, organ weight, AST, ALT, ALP, BUN, creatine and histologic change of liver and aorta were measured in db/db mouse after oral administration of CHP. CHP showed safety in cytotoxicity and toxicity of liver and kidney for logn time administration. CHP increased the DPPH and ABTS radical scavenging activity. CHP showed significant inhibitory effect on reactive oxygen species (ROS), and showed inhibitory effect on nitiric oxide(NO) compared to control group. CHP decreased cytokine IL-6 production significantly, and decreased IL-1β and TNF-α compared to control group. CHP decreased body and organ weitht, intake food, and glucose levels compared to control group. CHP decreased total cholesterol and triglyceride significantly, and decreased LDL-cholesterol levels and increased HDL-cholesterol levels compared to control group. CHP decreased atherogenic index and cardiac risk factor significantly. CHP increased serum insulin and GLP-1 compared to control group. In histologic examination, lipophagy in the liver and aorta decreased in CHP treated mice and the cell was regular and boundary of vessel wall was clear compared to control group. These results suggest that CHP is effective in antioxidation activity and treatment and prevention of hyperlipidemia, atherosclerosis, diabetes, ischemic heart disease, stroke and other cardiocerebrovascular disease.

• Journal of Life Science
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• 제17권12호
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• pp.1627-1633
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• 2007

c-Jun N-terminal kinase (JNK)-interacting protein 1 (JIP1), also known as Islet-brain 1 (IB1), is a scaffold protein that is highly expressed in neurons and pancreatic ${\beta}-cells$. In this study subcellular localization of JIP was investigated in cultured rat hippocampal neurons using an antibody that recognize all variants of JIP1, JIP-2 and JIP-3. The overall expression profile of JIP is punctate throughout soma and dendrites. Statistic analysis showed that $54.8{\pm}4.0%\;and\;94.1{\pm}4.5%$ of total JIP immunopuncta overlapped with those of excitatory postsynaptic markers SD-95 and ${\alpha}Camik$, respectively. In contrast, only $8.6{\pm}0.5%\;and\;7.3{\pm}0.5%$ of JIP clusters overlapped with those of inhibitory postsynaptic markers glycine receptor (GlyR) and gephyrin, respectively. JIP clusters overlapped or juxtaposed with SV2 but not GAD, markers for general and inhibitory nerve terminals, respectively. A substantial fraction $(29.3{\pm}1.0%)$ of flotillin immunopuncta, a marker for lipid rafts, clusters overlapped with those of JIP. In addition, JIP was highly expressed in some select ends of dendrites but minimal in axons. These data suggest important roles of JIP in excitatory postsynaptic sites, lipid rafts and dendritic ends.

• Journal of Korean Neurosurgical Society
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• 제30권sup2호
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• pp.281-288
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• 2001

Objectives : The purpose of this study is to evaluate the usefulness of SSEP monitoring during intracranial aneurysm surgery and compare the characteristics of wave change in relation to neurologic changes between ACA aneurysms and MCA aneurysms. Methods : During recent three years(between January 1997 and November 1999), intraoperative SSEP monitoring had been done in 63 operations for intracranial aneurysms. We had monitored the median nerve SSEP during surgery for aneurysms of MCA and the posterior tibial nerve SSEP for aneurysms of ACoA or ACA. A more than 50% reduction of any cortical SEP response was considered to be a significant SEP change, compared to its baseline value before the start of surgery. Changes in the SEPs were categorized as follows : Type IA, no significant amplitude changes without temporary clipping ; Type IB, no significant amplitude changes with temporary clipping ; Type II, significant changes with temporary clipping and complete return to control amplitude ; Type III, significant changes with temporary clipping and incomplete return to control amplitude ; Type IV, significant changes with temporary clipping and more decreased amplitude changes. Results : Among the 63 intraoperative monitoring, there were 37 cases of ACA aneurysms(An), and 26 of MCA An. The temporary proximal arterial occlusion during surgery were performed in 31(83.8%)cases of ACA An, 22(84.6%) of MCA An. Seven of the 31 ACA An(22.6%) and ten of the 22 MCA An(45.5%) had significant changes. The type were as follows : 4 patients with type II and 3 with type III in the ACA An ; 3 patients with type II and 3 with type III and 4 with type IV in the MCA An. In both group type II changes had no new postoperative neurological deficit. All 6 patients with type III had new neurological deficits ; However, One case in the ACA An and two cases in the MCA An. had transient neurologic deficit and improved markedly over the next two months. All 4 type IV changes in the MCA An. had permanant neurologic deficits. Two out of 30 cases(6.7%) in the ACA An. and one out of 16 cases(6.3%) in the MCA An. without significant amplitude change had new neurologic deficit postoperatively. Conclusion : Based on this study, Intraoperative SSEP monitoring during aneurysm surgery would provide useful information for detecting cerebral ischemia. SSEP response during surgery for MCA An. is more sensitive than ACA An. Otherwise, there were no meaningful difference in rate of false negativity.

• Journal of Nutrition and Health
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• 제36권10호
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• pp.1030-1035
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• 2003

This study was conducted to evaluate the safety of herbicide-resistant rice, a genetically modified organism (GMO) developed by the Rural Development Administration, in Sprague-Dawley rats. The nutrient content of herbicide-resistant polished and brown cooked rice was compared with that of conventional Ilpum polished and brown cooked rice to assess composition equivalence. Compositional analysis was performed to measure proximates, fiber, and minerals before animal feeding. Growing male rats were fed one of the following four diets for six weeks: Ilpum polished cooked rice (IP) and Ilpum brown cooked rice (IB) as a non-GMO and herbicide-resistant polished cooked rice (GP) and brown cooked rice (GB) as a GMO. We checked clinical symptoms (anorexia, salivation, diarrhea, polyuria, anuria, fecal change) every day, food intake, change of body weight twice a week, and serum biochemistry and organ weights after 6 weeks of experimental feeding among the four groups. Nutrient content of the herbicide-resistant rice was similar to that of the non-transgenic control and was within the published range observed for non-transgenic rice. We could not find any significant difference in the above-mentioned items as the index to be checked in the animals fed the GMO. These results suggest that the nutrient content of genetically modified herbicide-resistant rice is compositionally equivalent to that of conventional Ilpum rice and that growing male rats fed herbicide-resistant rice are no different from those fed Ilpum rice, non-GMO for 6 weeks.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• 제37권2호
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• pp.1-10
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• 2019

Based on the land system of Joseon Dynasty and contemporary perception of land, this study analyzed the reason Yun seon-do entered Bogildo Island and a characteristic of Buyong-dong garden. According to this analysis, this study interpreted the purpose that Yun seon-do had built Buyong-dong garden at a socio-economic perspective. The land such as Sarlimchontack(山林川澤) is recognized as a commonland in the Joseon Dynasty. Except the reserved area(禁山), however, actions to make commonland private through Ib-an(立案) and protecting graves was frequent. Bogildo Island was a place difficult to build a private garden because the island had been appointed a reserved forest to protect pine trees. Right after Byungjahoran, Yun seon-do entered the island on the pretext of protecting forests, and built Buyong-dong garden. The island was easy to move to manage private farms as well as possessed abundant forest resources and resources that have economic value such as fish and salt. It indicates that, unlike his pretext, economic benefits have also been considered.

• Journal of the Korean Wood Science and Technology
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• 제30권4호
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• pp.106-113
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• 2002

This research was performed to investigate the effect of moulding conditions on properties of the table top for student desk manufactured by wood waste materials. The table top manufactured using the mat mixed properly with fine particles and coarse particles generally showed better physical properties than those manufactured using either fine particles or coarse particles did/ Especially, highest performance for overlaying was observed when fine particles were used for face layer and coarse particles were used for core layer. The internal bond (IB) strength, the bending strength and thickness swelling increased with the increase of density. Most of physical properties were improved with the increase of hot pressing temperature. The best physical properties were found at 190℃ when the first hot pressing and the second hot pressing times were two minutes. However, the effect of the second hot pressing time of two minutes on the physical properties was not markedly different from that of one minute. This result suggests that the second hot pressing time of one minute is enough to increase the productivity in manufacturing the table top with the least decrease of the physical properties.

• Journal of the Korean Wood Science and Technology
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• 제30권1호
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• pp.25-33
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• 2002

Improvement of density profile and sound absorption capability of particleboard was attempted. Three types of hot pressing methods examined ; flat-platen pressing method (A-type pressing), hot pressing in forming box (B-type pressing), and hot pressing set up jagged caul in forming box (C-type pressing). The raw materials were larch(Larix leptolepis (S, et. Z.) Gorden) shavings, phenol formaldehyde resin, and the particleboard perforated with stair type. The physical and mechanical properties such as specific gravity, bending strength (MOR), internal bonding strength (IB) and sound absorption coefficients were examined. The results are summarized as follows : 1) The MOR and internal bonding strength of the board pressed in forming box were higher than those of flat-platen pressed board. 2) The minimum density to average density ratio in thickness direction which pressed in forming box showed about 923%, in the case of 35 mm commercial particleboard and 50 mm flat-platen pressed board, its values showed about 66.4% and 865% respectively. 3) Sound absorption coefficients of the particleboard perforated with stair type were higher than those of flat-plated pressed board and commercial particleboard.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• 제35권4호
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• pp.75-94
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• 2022

Objectives : To identify the active ingredient of Poncirus Trifoliata Immaturus and to explore the mechanism expected to potentially act on dermatitis accompanied by pruritus. Methods : We conducted the network pharmacological analysis. We selected effective ingredients among the active compounds of Poinciri Fructus Immaturus. We found the target protein of the selected active ingredient, disease(dermatitis accompanied by pruritus) and fexofenadine. Then we established the network between the proteins which Poinciri Fructus Immaturus and fexofenadine intersected with disease respectively, and the coregene was also extracted. After that, the active pathways in the human body involving the groups and coregenes were searched. Results : Total of 7 active ingredients were selected, and 202 target proteins were collected. There were 756 proteins related to inflammatory skin disease accompanied by pruritus, and 75 proteins were related to fexofenadine. 42 proteins crossed by Poinciri Fructus Immaturus with a disease, and 31 proteins crossed by fexofenadine with a disease. 12 proteins were found as a coregene from the proteins that cross Poinciri Fructus Immaturus and disease. Coregenes are involved in 'Nitric-oxide synthase regulator activity', 'Epidermal growth factor receptor signaling pathway'. 2 groups that extracted are invloved in 'Fc receptor signaling pathway', 'Central carbon metabolism in cancer', 'Phosphatidylinositol 3-kinase complex, class IB', and 'omega-hydroxylase P450 pathway'. Conclusion : It is expected that Poinciri Fructus Immaturus will be able to show direct or indirect anti-pruritus and anti-inflammatory effects on skin inflammation accompanied by pruritus in the future. And it is also expected to have a synergy effect with fexofenadine on skin disease.

• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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• 제6권9호
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• pp.59-70
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• 2016

The rapid development of the Chinese version of Twitter, the groom Weibo has become an important communication means for Chinese SNS users to obtain and share information. As a result, in China, the phenomenon of the power shift has emerged from the traditional opinion leaders to SNS opinion leasers. The relationship analysis of demographic variables of the Chinese SNS users and their Information on the relationship between keywords was made by utilizing the centrality analysis using Social Network Program NetMiner. China's SNS opinion leaders have general interest in daily activities with their families or friends rather than in social issues. And in case of SNS opinion leaders of high betweenness centrality, it was analyzed that general users was a key mediator role that organically out lead to the adjacent information. These properties are not independent of demographic variables, such as professional, therefore, the demographic characteristics of SNS opinion leaders showed a significant effect on the parameters of betweenness centrality. This study analyzed the characteristics of SNS users, especially opinion leaders in China by looking at the aspects of Chinese social phenomenon in terms of information. Through this study, we expect to provide basic information about the social characteristics of China through collective communication.

• Korean Journal of Environmental Agriculture
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• 제41권4호
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• pp.335-343
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• 2022

BACKGROUND: Antibiotics used in animal husbandry for disease prevention and treatment have resulted in the rapid progression of antibiotic resistant bacteria which can be introduced into the environment through livestock feces/manure, disseminating antibiotic resistant genes (ARGs). In this study, fecal samples were collected from the livestock farms located in Jeju Island to investigate the relationship between microbial communities and ARGs. METHODS AND RESULTS: Illumina MiSeq sequencing was applied to characterize microbial communities within each fecal sample. Using quantitative PCR (qPCR), ten ARGs encoding tetracycline resistance (tetB, tetM), sulfonamide resistance (sul1, sul2), fluoroquinolone resistance (qnrD, qnrS), fluoroquinolone and aminoglycoside resistance (aac(6')-Ib), beta-lactam resistance (bla_TEM, bla_CTX-M), macrolide resistance (ermC), a class 1 integronsintegrase gene (intI1), and a class 2 integrons-integrase gene (intI2) were quantified. The results showed that Firmicutes and Bacteroidetes were dominant in human, cow, horse, and pig groups, while Firmicutes and Actinobacteria were dominant in chicken group. Among ARGs, tetM was detected with the highest number of copies, followed by sul1 and sul2. Most of the genera belonging to Firmicutes showed positive correlations with ARGs and integron genes. There were 97, 34, 31, 25, and 22 genera in chicken, cow, pig, human, and horse respectively which showed positive correlations with ARGs and integron genes. In network analysis, we identified diversity of microbial communities which correlated with ARGs and integron genes. CONCLUSION(S): In this study, antibiotic resistance patterns in human and livestock fecal samples were identified. The abundance of ARGs and integron genes detected in the samples were associated with the amount of antibiotics commonly used for human and livestocks. We found diverse microbial communities associated with antibiotics resistance genes in different hosts, suggesting that antibiotics resistance can disseminate across environments through various routes. Identifying the routes of ARG dissemination in the environment would be the first step to overcome the challenge of antibiotic resistance in the future.