• 한국환경과학회지
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• 제13권6호
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• pp.513-518
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• 2004

3-weeks old Commelina was transferred to and grown in Hoagland solution (Control, $100\muM$ $Cd^{2+}$, $100\muM$ $Cd^{2+}$+ $100\muM$ IAA, $100\muM$ $Cd^{2+}$+ $100\muM$ IAA + 2 mM sucrose) for 3 weeks and then the effects of indole acetic acid (IAA) on the accumulation of $Cd^{2+}$ and growth of $Cd^{2+}$-treated Commelina were investigated. In the treatment of $Cd^{2+}$, $Cd^{2+}$ was uptaked to 1.74, and 51.36 ${\mu}g/g$ frwt. at the first week, but for three weeks, 0.51 and 34,53 ${\mu}g/g$ frwt, in leaf and stem respectively. When IAA was treated along with $Cd^{2+}$, $Cd^{2+}$ was uptaked to 0.18 and 8.63 ${\mu}g/g$ fiwt, at the first week, and for the incubation of 3 weeks, 0,51 and 45.0 ${\mu}g/g$ fiwt. in leaf and stem. In case of $Cd^{2+}$+IAA+sucrose, $Cd^{2+}$ was uptaked to 1.45 and 18.33 ${\mu}g/g$ frwt. at the first week, but for 3 weeks, 0,51 and 25.45 ${\mu}g/g$ fiwt. in leaf and stem. Likewise $Cd^{2+}$ uptake, the growth was also affected by $Cd^{2+}$ and IAA. During the incubation of 3 weeks, $Cd^{2+}$ reduced the stem growth about 8% in all weeks, but the treatment of IAA recovered the inhibition of stem growth caused by $Cd^{2+}$ to the degree of the control Therefore, it could be concluded that IAA altered the pattern of $Cd^{2+}$ uptake and the growth which were supposed to change $Cd^{2+}$ toxicity.

• 미생물학회지
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• 제50권1호
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• pp.15-21
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• 2014

Pseudomonas sp. P7014 박테리아를 통한 큰느타리버섯 균사체의 생육촉진에 관한 연구가 수행되었다. 박테리아 배양액으로 부터 분리한 ethyl acetate 분획물(F5)에는 성장촉진물질(GPC)이 함유되어 있음을 확인하였다. TLC, HPLC, NMR 및 MS/MS분석법으로 확인한 바, indole acetic acid (IAA)로 확인되었다. 큰느타리버섯 균사체는 성장촉진물질(GPC)이 첨가된 PDA와 PDB 배지에서 빠른 성장을 보였다. 성장촉진물질(GPC)의 농도는 1.0 nM로 매우 낮았지만, 확인된 tryptophan은 IAA의 전구체로써 IAA가 아민화된 형태였다. 이들 결과는 박테리아에서 분비된 성장촉진물질(GPC)은 IAA이었고 큰느타리버섯 균사체의 생육촉진에 중요한 역할을 하는 것으로 확인되었다.

• 한국응용과학기술학회지
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• 제39권6호
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• pp.831-838
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• 2022

농업 재배지 토양으로부터 auxin 생성세균 KSD16, KSD33 그리고 KSD36를 분리하였다. 분리균주 KSD16, KSD33 그리고 KSD36 는 16S rRNA 유전자 계통분석을 통해 Arthrobacter 속으로 분류되었다. 이들 균주는 R2A배지에 0.1% L-tryptophan를 첨가한 배지에서 28℃, 48 시간 배양한 결과, auxin의 일종인 indole-3-acetic acid (IAA)를 204.4 mg L^-1 생성하는 것으로 확인되었다. IAA 생성세균의 녹두종자 발아에 미치는 영향을 확인한 결과, Arthrobacter 속 균주 KSD16, KSD33 그리고 KSD36 는 대조군에 비해 뿌리길이와 발근수가 증가하였다. Arthrobacter 속 균주의 녹두 성장촉진 효과를 확인한 결과, 녹두의 발아율이 대조군보다 73.4 % 증가하는 특징을 나타내었다.

• Journal of Applied Biological Chemistry
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• 제44권4호
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• pp.173-176
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• 2001

This report concerns the role of indole-3-acetic acid (IAA) in bacterial pustule disease of soybean. Pustule production in soybean leaves caused by Xanthomonas axonopodis pv. glycines was accompanied by a drastic increase in IAA content of host tissues. The phytopathogenic bacterium synthesized IAA in a tryptophan concentration-dependent manner when grown in a defined minimal medium. In complex media, however, the pathogen showed no response to tryptophan feeding, implying that the bacterial biosynthetic machinery of IAA is strictly regulated by nutrient availability of its growth environments. The results may suggest that IAA of bacterial origin and tryptophan of plant origin be involved in the process of pustule symptom development in soybean.

• 한국환경복원기술학회지
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• 제6권6호
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• pp.25-28
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• 2003

This study was conducted to development of rapid propagation method by leaf cuttings in Hibiscus hamabo native to southern seaside of Korea, and special object of this study was to determine the effects of IAA, IBA, and NAA on rooting in leaf cuttings of H. hamabo. Rooting was promoted by dipping treated with IAA and IBA. And rooting percentage was greatest at 1,000 and 2,000$mg{\cdot}L^{-1}$ IBA. At higher concentrations of IAA and IBA, more adventitious roots were developed. Also, IAA at high concentrations (above 2,000$mg{\cdot}L^{-1}$) and IBA ranged from 500~2,000$mg{\cdot}L^{-1}$ promoted on root number and rootingratio. However, root formation in Hibiscus leaf cuttings inhibited by NAA application.

• Journal of Plant Biology
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• 제34권3호
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• pp.239-244
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• 1991

Ca2+ is implicated as a second messenger in coupling various stimuli such as hormone, gravity and light. The determine whether or not plant hormones mobilize calcium with different action, we investigated the cytosolic Ca2+ changes by IAA and zeatin in the protoplasts isolated from elongating mesocotyl of maize. IAA increased the influx of Ca2+ due to the calcium channel opening, which was confirmed by using verapamil, calcium channel blocker. On the other hand, zeatin increased the cytosolic Ca2+ by promoting the efflux of Ca2+ derived from cellular organelles. These results suggest that different calcium flux induced by IAA and zeatin plays a role in appropriate response resulting in increase of cell elongation or repression cell elongatoin.

• 식물조직배양학회지
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• 제26권1호
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• pp.27-30
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• 1999

복숭아 품종 백미조생, 대구보, 유명의 기내 신초 증식 및 발근에 미치는 생장조절제의 종류 및 농도의 영향을 알아보기 위하여 일련의 실험을 실시하였다. 기내 신초 대량증식에 효과적인 cytokinin은 2.0 mg/L BA처리였으며, BA와 auxin과의 혼용처리 결과 2.0mg/L BA와 1.0mg/L IAA혼용처리가 백미조생 품종의 신초 증식률에 가장 효과적이었다. 배양된 신초의 기내발근에 효과적인 auxin은 IAA였으며,그 농도는 백미조생에서는 5.0mg/L, 대구보에서는 3.0mg/L였다.

• Journal of Applied Biological Chemistry
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• 제43권4호
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• pp.269-272
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• 2000

The ginseng growth-promoting bacterium Pseudomonas fluorescens KGPP 207 synthesized indole-3- acetic acid (IAA) from L-tryptophan, indole-3-pyruvic acid (IPyA), and indole-3-acetaldehyde (IAAld), but not from indole-3-acetamide (lAM) and other intermediates of various IAA biosynthetic pathways in the experiment with indole compound supplemented cell suspensions. TLC, HPLC, and GC-MS analyses revealed the presence of IPyA, indole-3-ethanol, indole-3-lactic acid and its methyl ester, IAA and its methyl, and ethyl esters in the culture supernatant of the bacterium. IAAld was detected in the supernatant using sodium bisulfite and TLC. The results indicate that unlike gall-forming bacteria which can synthesize IAA by lAM, the indole-3-pyruvic acid pathway is the route for IAA biosynthesis in this beneficial strain of P. fluorescens.

• 농업과학연구
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• 제43권1호
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• pp.100-108
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• 2016

An auxin-producing bacteria (strain 5-1) was isolated from button mushroom compost in Boryeong-Si, Chungcheongnam-Do. The 5-1 strain was classified as a novel strain of Enterobacter aerogenes based on chemotaxonomic and phylogenetic analyses. The isolated E. aerogenes 5-1 was confirmed to produce indole-3-acetic acid (IAA), one of the auxin hormones, using TLC and HPLC analyses. When the concentration of IAA was assessed by performing HPLC quantitative analysis, a maximum concentration of IAA of $109.9mgL^{-1}$ was detected in the culture broth incubated in R2A medium containing 0.1% L-tryptophan for 24 h at $35^{\circ}C$. Acidification of the culture was deemed caused by an increase of IAA because a negative relationship between IAA production and pH was observed. Supplementation with a known precursor of IAA production, L-tryptophan, appeared to induce maximal production at 0.1% concentration, but it reduced production at concentrations above 0.2%. To investigate the growth-promoting effects to crops, the culture broth of E. aerogenes 5-1 was used to inoculate water cultures and seed pots of mung bean and lettuce. In consequence, adventitious root induction and root growth of mung bean and lettuce were two times higher than those of the control.

• Journal of Microbiology and Biotechnology
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• 제28권9호
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• pp.1511-1516
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• 2018

In this study, we investigated Indole-3-acetic acid (IAA) production by a rice phylloplane bacteria, Enterobacter sp. DMKU-RP206, using sweet whey as a feed stock instead of lactose. We succeeded in using sweet whey for Enterobacter sp. DMKU-RP206 to produce 3,963.0 mg IAA/l with the optimal medium containing 1.48% sweet whey, 1.42% yeast extract and 0.88% $\text\tiny{L}$-tryptophan. The medium pH was adjusted to 6 and the culture conditions were shaking at 200 rpm on an orbital shaker at $30^{\circ}C$ for 3 days. We also evaluated the effect of IAA in culture filtrates of Enterobacter sp. DMKU-RP206 on the promotion of jasmine rice growth in a pot experiment. Compared with the negative control (without IAA), the result showed that biosynthetic IAA produced by Enterobacter sp. DMKU-RP206 significantly increased the growth of jasmine rice (Oryza sativa L. cv. KDML105) in terms of length and dry weight of shoot. This work thus reveals the impact of IAA produced by Enterobacter sp. on the promotion of jasmine rice growth.