• Title/Summary/Keyword: I.C.U.

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The Study on Classification of Aromatic Herbs in Oriental Medicine for Aromatherapy (방향성(芳香性) 본초(本草) 분류를 통한 향기요법(香氣療法) 연구(硏究))

  • Uhm, Ji-Tae;Kim, Kyoung-Shin;Kang, Jung-Soo;Kim, Byoung-Soo
    • Journal of Haehwa Medicine
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    • v.20 no.1
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    • pp.39-50
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    • 2011
  • In western, aromatherapy uses essential oils to prevent and treat disease, and is one of the ways to stay healthy. In oriental medicine, there was also a similar medical treatment using aromatic herbs for a long ago. The exisiting research of oriental medical herbs is mainly focused on the theory of qi and flavor(氣味論). But on aromatherapy used with aromatic herbs, it is necessary to study the aromatic herbs by aroma. So "xi$\bar{a}$ng p$\check{u}$, 香譜" and "b$\check{e}$n c$\check{a}$o g$\bar{a}$ng m$\grave{u}$, 本草綱目" mainly contain the study about clinical effects of aromatic herbs. And "b$\check{e}$n c$\check{a}$o p$\check{i}$n hu$\grave{i}$ j$\bar{i}$ng y$\grave{a}$o, 本草品彙精要"mainly explains the effects of medical herbs with five aroma classification of x$\bar{i}$ng(腥), sh$\bar{a}$n(膻), xi$\bar{a}$ng(香), ch$\grave{o}$u(臭), xi$\check{u}$(朽). The former one makes more clinical uses of medical herbs, and the latter one grows up the theory of medical herbs. This study is expected to have a good influence on aromatherapy and classification of oriental medical herbs.

High-Level Expression of an Aspergillus niger Endo-$\beta$-1,4-Glucanase in Pichia pastoris Through Gene Codon Optimization and Synthesis

  • Zhao, Shumiao;Huang, Jun;Zhang, Changyi;Deng, Ling;Hu, Nan;Liang, Yunxiang
    • Journal of Microbiology and Biotechnology
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    • v.20 no.3
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    • pp.467-473
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    • 2010
  • To improve the expression efficiency of recombinant endo-$\beta$-1,4-glucanase in P. pastoris, the endo-$\beta$-1,4-glucanase (egI) gene from Aspergillus niger was synthesized using optimized codons. Fourteen pairs of oligonucleotides with 15 bp overlap were designed and the full-length syn-egI gene was generated by two-step PCR-based DNA synthesis. In the synthesized endo-$\beta$-1,4-glucanase gene syn-egI, 193 nucleotides were changed, and the G+C content was decreased from 54% to 44.2%. The syn-egI gene was inserted into pPIC9K and transformed into P. pastoris GS115 by electroporation. The enzyme activity of recombinant P. pastoris stain 2-7# reached 20.3 U/ml with 1% barley $\beta$-glucan and 3.3 U/ml with 1% carboxymethylcellulose (CMC) as substrates in shake flasks versus 1,270.3 U/ml and 220.7 U/ml for the same substrates in 50-1 fermentors. The molecular mass of the recombinant protein was approximately 40 kDa as determined by SDS-PAGE analysis, the optimal temperature for recombinant enzyme activity was $70^{\circ}C$, and the optimal pH was 5.0 when CMC was used as the substrate.

SELF-ADJOINT INTERPOLATION ON Ax = y IN CSL-ALGEBRA ALGL

  • Kang, Joo-Ho;Jo, Young-Soo
    • Journal of applied mathematics & informatics
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    • v.15 no.1_2
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    • pp.503-510
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    • 2004
  • Given vectors x and y in a Hilbert space, an interpolating operator is a bounded operator T such that Tx = y. An interpolating operator for n vectors satisfies the equation $Tx_i\;=\;y_i,\;for\;i\;=\;1,\;2,\;\cdots,\;n$. In this paper the following is proved: Let H be a Hilbert space and L be a commutative subspace lattice on H. Let H and y be vectors in H. Let $M_x\;=\;\{{\sum{n}{i=1}}\;{\alpha}_iE_ix\;:\;n\;{\in}\;N,\;{\alpha}_i\;{\in}\;{\mathbb{C}}\;and\;E_i\;{\in}\;L\}\;and\;M_y\;=\;\{{\sum{n}{i=1}}\;{\alpha}_iE_iy\;:\;n\;{\in}\;N,\;{\alpha}_i\;{\in}\;{\mathbb{C}}\;and\;E_i\;{\in}\;L\}. Then the following are equivalent. (1) There exists an operator A in AlgL such that Ax = y, Af = 0 for all f in ${\overline{M_x}}^{\bot}$, AE = EA for all $E\;{\in}\;L\;and\;A^{*}\;=\;A$. (2) $sup\;\{\frac{{\parallel}{{\Sigma}_{i=1}}^{n}\;{\alpha}_iE_iy{\parallel}}{{\parallel}{{\Sigma}_{i=1}}^{n}\;{\alpha}_iE_iy{\parallel}}\;:\;n\;{\in}\;N,\;{\alpha}_i\;{\in}\;{\mathbb{C}}\;and\;E_i\;{\in}\;L\}\;<\;{\infty},\;{\overline{M_u}}\;{\subset}{\overline{M_x}}$ and < Ex, y >=< Ey, x > for all E in L.

Effects of Covering Parts of Body with Garments on Human Thermoregulation and Sensation (신체의 부위별 피복이 체온조절 및 주곤적인 감각에 미치는 영향(I))

  • 이종민;이순원
    • Journal of the Korean Society of Clothing and Textiles
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    • v.18 no.2
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    • pp.273-282
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    • 1994
  • The physiological significances of the upper and lower body on thermoregulation and sensation were studied in this paper. Experiments were carried out on 4 females in a climatic chamber conditioned at 1) $25^{\circ}C\rightarrow35^{\circ}C\rightarrow25^{\circ}C$, 2) $25^{\circ}C\rightarrow15^{\circ}C\rightarrow25^{\circ}C$, both with 50% R.H., covering the upper body (U) or lower body (L) with garments. 1. When the upper or lower body is covered or exposured respectively, the mean skin tempterature of upper body is higher than that of lower body. And upper body is more easily influenced by the environmental temperature than lower body. It means the skin temperatures of the upper body change faster than those of the lower body following the environmental changes. 2. In U and L, the skin temperatures of the upper limbs (thighs, upper arms) are lower than those of the peripherals (hands, feet). 3. Warm sensations and skin temperatures of the upper body showed high correlation and it was the case with cold sensations and skin temperatures of the lower body. 4. In high temperature condition $(25^{\circ}C\rightarrow35^{\circ}C\rightarrow25^{\circ}C)$, mean skill temperature and rectal temperature in L were lower than in U. This lower rectal temperature in L is probably due to the insulation of the lower body with garments that promotes the heat radiation only in the high temperature environment.

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Changes in Cytosolic $Ca^{2+}$ but not in cGMP Contents May be more Important to Nitric Oxide-Mediated Relaxation in Depolarized Vascular Smooth Muscle

  • Lee, Hyun-Seok;Chang, Ki-Churl
    • The Korean Journal of Physiology and Pharmacology
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    • v.2 no.1
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    • pp.63-68
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    • 1998
  • Nitric oxide (NO)-mediated relaxation in vascular smooth muscle involves not only activation of guanylate cyclase but also hyperpolarization of the membrane. It has been shown that depolarization decreases the [$Ca^{2+}$] sensitivity of myosin light chain kinase in arterial smooth muscle, and nitric oxide (NO)-mediated relaxation was attenuated in this situation. However, why potassium inhibits or attenuates the action of EDRF/NO is not clear. Therefore, we investigated the magnitude of relaxation and cGMP contents using measures known to release NO, such as photorelaxation, photo activated NO-mediated relaxation, and NO-donor (SNP)-mediated relaxation in porcine coronary arterial rings in which contractile conditions were made by different degree of depolarization, i.e., contraction in response to U46619 or U46619 plus KCl. In all cases, the magnitude of relaxation was significantly greater (P<0.05) in U46619-contracted rings than in U46619+KCl-contracted ones. Although accumulation of cGMP was evident with three measures employed in the present study, no difference was found in cGMP contents between U46619 and U46619+KCl conditions, indicating that the diminished relaxation in KCl containing solution is cGMP-independent mechanism(s). To understand this further, cytosolic $Ca^{2+}$ changes due to NO were compared in rat thoracic aorta by exploiting photoactivated NO using streptozotocin (STZ) that was contracted with either NE or KCl. Fura-3 $[Ca]_{cyt}$ signal caused by NO was small and transient in high $K^+$-, but large and sustained in NE-contracted aorta. The inhibitory potency of STZ expressed in terms of $IC_{50}$ was 5.14 and 3.88 ${\mu}M$ in NE and in high $K^+$, respectively. These results suggest that modification of the cellular mobilization of $Ca^{2+}$ rather than cGMP levels may be an important mechanism for the NO-mediated relaxation when vascular membrane is depolarized, such as atherosclerosis and hypertension.

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A Study on the Mechanochemical Effects of Dickite (Dickite의 메카노케미칼 효과에 관한 연구)

  • Ryu, Ho-Jin
    • Korean Journal of Materials Research
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    • v.7 no.2
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    • pp.152-156
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    • 1997
  • In this paper, I meritioned ,I study on thc rnechanochernic;~l effec ts of grountl iljckite To investigate the mechanochemical effects of ground tlickite 1,y planet,~r\- mill. a structul-ill i~nalvsis of dickite prepared with different grinding time has been made 11y X-ray diffraction and ilifierenti,~l tl~i.~-ni;~i analysis. 'l'he $SiO_{4}$ hecira was unchanged bl- milling and remained ;is the local ordering unit s t t - ~ c ~ u ~ - c ~ s in the ground dickitc samples I ) v analyzing the radial distribution function. On the other hand, with an increi~se of the grinding time a decrease of coordination number and atomic distance bet\vccn aluminum and oxygen hi1c.r l~een octurreil, and Li~~antitativcl\. coniirrned them This result corresponded to the local c,hange around aliimi~iuni I>V tliftercntii~l t1ii:rm;il ani~lysis. Therefore, the mechanochemical phenomina of ground tiickite werc3 h e to the local c~l~mge around aluminum \IT. the prolonged grinding.

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Study on the fabrication and the growth mechanism of Bi-2223 superconducting phase by diffusion method (확산법에 의한 Bi-2223 초전도상의 제조 및 성장기구에 관한 연구)

  • 최성환;최효상;한태희;황종선;한병성
    • Electrical & Electronic Materials
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    • v.7 no.4
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    • pp.281-288
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    • 1994
  • According to spread volume of B(BiPbCuO) layer, composition ratio and each stage of sintering process, we studied stability of high Tc superconductor phase and generation and growth movement of superconducting phase. The dual layer composed of SrCaCuO and BiPbCuO compound were prepared to develop the Bi-2223 superconductor[108K] through interaction and diffusion during sintering process. The dual layer samples were sintered at 830.deg. C for 0-210 hours. From the result, the optimum conditions were : spread volume(A:B=1:0.6), sintering time(210h) and composition ratio(A:S $r_{2}$C $a_{2}$C $u_{2}$- $O_{x}$, B:B $i_{1.9}$P $b_{0.5}$C $u_{3}$ $O_{y}$) at 830.deg. C.. C.C.C.

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Quantification of Bowman-Birk Protease Inhibitors in Soybeans and Soybean Products by Competitive Enzyme-Linked Immunosorbent Assay (경합 Enzyme-Linked Immunosorbent Assay에 의한 대두 및 대두가공제품 중의 Bowman-Birk Protease Inhibitors의 함량 분석)

  • Kim, Sung-Ran;Shon, Dong-Hwa;Kim, Su-Il;Hong, Hee-Do
    • Applied Biological Chemistry
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    • v.42 no.4
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    • pp.310-316
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    • 1999
  • BBPI contents in domestic soybean and soybean products were investigated by the measurement of chymotrypsin inhibiting activity(C.I.A) and competitive ELISA method. In order to produce polyclonal antibody, BBPI was purified from soybean trypsin-chymotrypsin inhibitor by ion exchange chromatography and electrophoretic gel slicing. Rabbit anti-BBPI polyclonal antibody was produced with the purified BBPI as immunogen. This antibody showed relatively specific binding to BBPI and then used for the establishment of competitive ELISA method to measure BBPI contents in extracts of soybean and soybean products. The standard curve for the measurement of BBPI in soybean extracts was drawn up within the range 0.03 to $30\;{\mu}g/ml$ of BBPI. The C.I.A. and BBPI contents of 12 soybean cultivars were $8,462{\sim}12,428\;U/g$ and $482{\sim}692\;mg%$, respectively. The C.I.A. and BBPI contents were not detected in most of soybean products except soybean sprouts, which contained $10,695{\sim}13,249\;U/g$ of C.I.A. and $529{\sim}803\;mg%$ of BBPI.

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Heat Transfer Characteristics of the U-shape Heat Pipe using Working Fluid of PFC (PFC 작동유체 사용 U형 히트파이프의 열전달특성 연구)

  • 이기우;박기호;전원표
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.13 no.8
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    • pp.796-802
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    • 2001
  • The purpose of the present study is to examine the heat transfer characteristics of the U-shape heat pipe for the cooling of semiconductor in subway train. Perflouro-carbon(PFC) was used as working fluid. Temperature distribution on the surface and heat transfer coefficients were investigated according to the working fluid volume percent and heating rate. The results were as follows; Optimum volumetric percent of working fluid was from 80% to 90%, and hat transfer coefficients of evaporation and condensation were as follows, respectively. $\hbar_ie=0.37\times(\frac{P_i}{P_O})$l_c}^0.3$,$\hbar_ic-4.2(\frac{\kappa_l^3p_l^2gh_fg}{\mu_lq_c_l_c}^\frac{1}{3}

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