• Parasites, Hosts and Diseases
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• 제57권6호
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• pp.699-704
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• 2019

Anisakiasis (anisakidosis) refers to a foodborne zoonosis caused by ingesting raw or undercooked marine fish or cephalopods infected with anisakid larvae. The present study was performed to investigate the prevalence of anisakid larvae in anchovies (Engraulis japonica) purchased from 2 local markets in Gyeongsangnam-do, the Republic of Korea (=Korea), during 2018-2019. Anchovies were transported to our laboratory and examined by pepsin-HCl artificial digestion technique followed by microscopic observations and molecular analyses. The overall prevalence of anisakid larvae was 19.5% (39/200), from which a total of 51 larvae (av. 1.3 larvae/infected anchovy) were recovered. Sequencing of the larvae targeting the ITS region, including ITS1, 5.8S rRNA, and ITS2 genes confirmed the species of larvae as Anisakis pegreffii (54.9%; 28/51), Hysterothylacium sinense (23.5%; 12/51), and Hysterothylacium aduncum (21.5%; 11/51). The results suggested that anchovies could be a potential source of human anisakiasis in Korea.

• Parasites, Hosts and Diseases
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• 제57권2호
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• pp.207-211
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• 2019

Anisakiasis is a zoonotic disease induced by anisakid nematodes, and endoscopic inspection is used for a diagnosis or remedy for it. Anisakis simplex, Anisakis physeteris, and Pseudoterranova decipiens had been reported to be the major species causing human infections, particularly, in Japan. However, in Korea, recent studies strongly suggested that Anisakis pegreffii is the major species of human infections. To support this suggestion, we collected anisakid larvae (n=20) from 20 human patients who were undergone gastrointestinal endoscopy at a health check-up center in Korea, and molecular identification was performed on the larvae using PCR-RFLP analysis and gene sequencing of rDNA ITS regions and mtDNA cox2. In addition, anisakid larvae (n=53) collected from the sea eel (Astroconger myriaster) were also examined for comparison with those extracted from humans. The results showed that all human samples (100%) were identified as A. pegreffii, whereas 90.7% of the samples from the sea eel were A. pegreffii with the remaining 9.3% being Hysterothylacium aduncum. Our study confirmed that A. pegreffii is the predominant species causing human anisakiasis in Korea, and this seems to be due to the predominance of this larval type in the fish (sea eels) popularly consumed by the Korean people. The possibility of human infection with H. aduncum in Korea is also suggested.

• 한국어병학회지
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• 제25권3호
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• pp.189-197
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• 2012

2010~2012년까지 남해안 연안에서 서식하는 총 9목 30과 50종 243마리의 자연산 해산어류를 대상으로 아니사키스과 선충 유충의 감염현황을 조사하였다. 아니사키스과 선충 유충의 감염률은 10.7% (26/243마리) 로 나타났으며, 2010년 여수 시료에서 7.4% (7/95마리), 2011년 여수와 제주 시료에서 각각 22.7% (5/22마리) 와 8.2% (5/61마리), 2012년 완도 시료에서 40.9% (9/22마리) 로 나타났다. 2011년 통영 및 완도 시료에서는 아니사키스과 선충 유충이 발견되지 않았다. 총 10종의 어류 (성대, 쏨뱅이, 넙치, 볼락, 삼치, 흰꼬리볼락, 점농어, 황놀래기, 독가시치 및 노래미) 26마리에서 분리된 89마리의 아니사키스과 선충 유충을 ITS gene을 타겟으로 한 PCR-RFLP 및 염기서열 분석을 통해 동정한 결과, 6종의 아니사키스충이 동정되었고 성대, 쏨뱅이, 넙치 및 볼락에서 상대적으로 높은 감염율을 보였다: Anisakis pegreffii (53.9%, 48/89 아니사키스 유충), Hysterothylacium aduncum (38.2%, 34/89), H. fabri (3.4%, 3/89), hybird (A. simplex X A. pegreffii) (2.4%, 2/89), A. simplex (1.1%, 1/89), Raphidascaris lophii (1.1%, 1/89). 유충의 감염은 혼합감염 (19.2%, 5/26마리) 보다는 단독감염 (80.8%, 21/26마리) 이 높게 나타났다.