• Journal of Korean Neurosurgical Society
• /
• 제42권6호
• /
• pp.470-474
• /
• 2007

Objective : The brain is dependent on glucose as an energy source. Intricate homeostatic mechanisms have been implicated in maintaining the blood glucose concentration in the brain. The aim of this study is to find the way to identify the metabolic proteins regulating the glucose in rat hypothalamus. Methods : In this study, we analysed the secretome from rat hypothalamus in vivo. We introduced 500 nM of insulin into the rat hypothalamus. The chromatographic patterns of the secretome were identified, after which Mass Spectrometry-Mass Spectrometry (MS-MS) analysis was performed. Results : In Liquid Chromatography-Mass Spectrometry (LC-MS) analysis, 60 proteins were identified in the secretome. Among them, 8 novel proteins were unveiled and were associated with the energy metabolism of insulin signaling in mitochondria of rat hypothalamic neuron. Nineteen other proteins have unknown functions. These ligands were confirmed to be secreting from the rat hypothalmus on insulin signaling by western blotting. Conclusion : The hypothalamus is the master endocrine gland responsible for the regulation of various physiological and metabolic processes. Proteomics using LC-MS analysis offer a efficient means for generating a comprehensive analysis of hypothalamic protein expression by insulin signaling.

• 한국동물학회지
• /
• 제37권1호
• /
• pp.130-136
• /
• 1994

Although alteration in protein phosphorylation by specific protein kinases is of importance in transducing cellular signals in a variety of neural/endocrine systems, little is known about protein phosphorylation in the hvpothalamus. The present study aims to explore whether activation of the second messenger-dependent protein kinases affects phosphorylation of specific proteins using a cell free phosphorylation system followed by SDS-polvacrylamide gel electrophoresis. Cytoplasmic fractions derived from hvpothalami of immature rats were used as substrates and several activators and/or inhibitors of CAMP-, phosphatidylinositol- and Ca2+-calmodulin-dependent protein kinases were assessed. Many endogenous proteins were extensively phosphorylated and depending on the signal transduction pathways, phosphorvlation profiles were markedly different. The present data indicate that extracellular signals may affect cellular events through protein phosphorylation by second messengers-protein kinases in the rat hypothalamus.

• 한국동물학회지
• /
• 제32권3호
• /
• pp.275-280
• /
• 1989

본 연구는 한우에서 적출한 시상하부조직을 superfusion 하여 체외배양 하면서 in vitro LHRH의 내인성 분비양상을 조사하였으며, 한우의 in vitro LHRH 분비양상과 수입소인 홀스타인 젖소의 LHRH 분비양상을 비교하였다. 시상하부의 median eminence조직을 적출하여 조각을 내어서 superfusion chamber에 넣은 후 37$^{\circ}C$ 배양기에서 배양하였다. superfusion은 4시간동안 계속되었으며, 10분간격으로 분획을 받아내어 LHRH 분비능을 LHRH방사면역 측정법으로 측정하였다. LHRH 분비 양상의 특성은 PULSAR algorithm으로 분석하였다. 한우와 수입 젖소에 있어서 내인성 LHRH분비는 맥동적인 양상을 보였다. 한우에 있어서 LHRH 분비의 평균분비, 펄스의 진폭과 그 간격은 11.08 $\pm$ 1.50 pg/min/mg x 10-$^2$, 21.43 1 7.28 pg/mg x 10-$^2$, and 39.42 $\pm$ 3.08 min이었고 이 값은 홀스타인 젖소에서의 측정치와 거의 유사하였다. 이와 같은 한우의 펄스 발신기의 기본적인 특징은 한우의 신경내분비적 연구에 중요하리라 사료된다.