• 식물조직배양학회지
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• 제26권2호
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• pp.77-83
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• 1999

Agrobacterium tumefaciens $A_4$T에 의해 형질전환된 사리풀 (Hyoscyamus niger L.)의 모상근으로부터 tropane alkaloids (scopolamine, hyoscyamine)의 생산성 향상을 위한 최적배지, 배양기간 및 탄수화물의 영향을 조사하였다. Tropane alkaloids의 생산성이 우수한 모상근 (HNl8, HN57)의 최적 배지는 SH배지이며, 모상근의 생장, tropane alkaloids 함량 및 생산성에서 조사한 6가지 배지보다 높게 나타났다. Tropane alkaloids생산을 위한 최적 배양기간은 HNl8 clone이 6주, HN57 clone은 5주로 나타났으며, 배양기간에 따라 scopolamine의 함량은 계속적으로 증가하는데 반하여 hyoscyamine의 함량은 일정 수준을 유지하는 것으로 나타났다. 두 세포주는 sucrose농도가 7%까지 증가할수록 생장률이 증가하는 반면, tropane alkaloids 함량은 감소하였으며, tropane alkaloids생산성은 3% sucrose처리구에서 가장 높게 나타났다. HN18 clone의 경우 최적 sucrose(3%)및 dextrose (2%)에서 유사한 tropane alkaloids 생산성을 나타내었다. 하지만 HN57 clone은 sucrose 처리구에 비하여 dextrose 처리구에서 매우 낮은 tropane alkaloids생산성을 나타내었다.

• 한국약용작물학회지
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• 제13권5호
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• pp.237-241
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• 2005

Scopolamine and hyoscyamine are important anticholinergic compounds obtained from Hyoscyamus niger. Adventitious roots induced from rhizome of H. niger and roots were cultured in SH medium supplemented with 3% (w/v) sucrose and 0.5 mg/L IBA. Roots were grown rapidly after 10 days of cultures. Scopolamine production was increased 7 times and hyoscyamine production was increased 12 times after 10 day of cultures. SH medium was best in root growth. But, highest scopolamine productivity was observed in WPM medium, followed White medium and best hyoscyamine productivity was resulted in MS medium. Sucrose was increased scopolamine and hyoscyamine production were increased the medium supplemented by sucrose comparing to than those by other carbon sources.

• 한국산림과학회지
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• 제98권2호
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• pp.142-147
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• 2009

Hyoscyamus species is sources of the hypnotic and sedative drugs hyoscyamine and scopolamine. Single cells of Hyoscyamus niger were dissociated from suspension cultures and adventitious roots obtained from single-cell clones which were cultured on B5 medium containing 3% (w/v) sucrose, 0.1 mg/L IBA and 0.4% (w/v) gelrite. H. niger adventitious root lines showed wide variation in tropane alkaloids production and growth. An effective selection of 200 root lines was made possible by the application of the 'Dragendorff's reagent' for qualitative detection of the alkaloids from root. A high correlation coefficient (r=0.9390) was observed between the values obtained with the two methods based on HPLC and Dragendorff's reagent analysis. Among the selected roots, the highest scopolamine content was 16.64 mg/g DW (Hn-59), which was 8.82-fold more productive than the lowest alkaloid producing line (Hn-25). Here, we established an efficient selection method on tropane alkaloids production and suggest that the Dragendorff's reagent is of great practical value in selection of invisible compounds.

• 한국약용작물학회지
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• 제12권2호
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• pp.118-122
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• 2004

We have investigated that a couple of soft ferrite ceramic powders having a spinal structure have shown the effect on growth and secondary metabolites production of some medicinal plants cultured in vitro. The addition of the ceramic powders as bare state to culture medium has stimulated the growth of Achyranthes japonica callus and plantlet, adventitious root of Hyoscyamus niger and Platycodon grandiflorum hairy root about 65, 75, 150 and 50%, respectively. Whereas Centella asiatica callus and plantlet, Scopolia parviflora hairy root, and Hyoscyamus albus adventitious root were not affected markedly. Moreover, the ceramic powder has enhanced the growth of H. niger adventitious roots even under conditions of irradiating alone without any direct contact between ceramic powder and media. Based on growth stimulation effect, the ceramic powders have enhanced the gross production of tropane alkaloid in H. niger adventitious root, and polyacetylene in P. grandiflorum hairy root about 35 and 30%, respectively.

• KSBB Journal
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• 제13권5호
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• pp.530-534
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• 1998

The addition of the ceramic powder as state of bare in culture medium has stimulated the growth of Achyranthes japonica in both the disorganized cell and the plantlet. The grwoth rate of Hyoscyamus niger adventitious root and Pylatycodon grandiflorum hairy root was enhanced up to 100 and 250%, respectively, even though Scopolia parviflora hairy root and Hyoscyamus albus adventitious root were not. The ceramic powder has enhanced the growth of H. niger adventitious root even in a test tube immersed into its culture medium to irradiate alone without any direct contact. The ceramic powder seems to have a significant role on both the growth and the physiological action of some plants.

• 대한약학회:학술대회논문집
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• 대한약학회 2001년도 Proceedings of the Pharmaceutical Society of Korea
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• pp.214.3-215
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• 2001

• 한국동물학회:학술대회논문집
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• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
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• pp.365.2-365
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• 1998

No Abstract, See Full Text

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.568-570
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• 2003

The Hyoscyamus niger hyoscyamine $6{\beta}-hydroxylase$ (H6H, EC 1.14.11.11) gene was introduced into the genome of a Scopolia parviflora by the binary vector system using the disarmed Agrobacterium rhizogenes strain KCTC 2703. Expression of H6H enzyme which are involved in alkaloids pathway by western blot analysis using proteins extracted from leaf, stem flower, branch root and main root were examined The enzyme expression was found only in the roots, with no expression in leaf, stem and flower. The alkaloids contents were the most higher in root and then leaf and stem has very small amount of alkaloid contents were analyzed by HPLC. The expression level of H6H in transgenic plants were two or more times than wild type plants. In transgenic plant which constitutively expresses H6H enzyme, high concentration of scopolamine was accumulated.

• 식물분류학회지
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• 제46권1호
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• pp.60-64
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• 2016

Scopolia parviflora of the family Solanaceae is an endemic species of Korea and a traditional Korean medicinal plant. The plastid genome was sequenced by next-generation sequencing (NGS) method. The characterized cp genome is 156,193 bp in size; the large single-copy (LSC) region is 86,364 bp, the inverted repeat (IR) is 25,905 bp, and the small single copy (SSC) region is 18,019 bp. The overall GC content of the plastid genome amounts to 37.61%. The cp genome contains 113 genes and 21 introns, including 80 proteincoding genes, four RNA genes, 30 tRNA genes, 20 group II introns, and one group I intron. A phylogenetic analysis showed that Scopolia parviflora was closely related to Hyoscyamus niger.

• 생약학회지
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• 제31권1호
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• pp.23-27
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• 2000

The effect of seventy kinds of medicinal plants on the activities of GABA-metabolizing enzymes as glutamate dehydrogenase I (GDH I), glutamate dehydrogenase II (GDH II), GABA transaminase (GABA-T), succinic semialdehyde dehydrogenase (SSADH) and succinic semialdehyde reductase (SSAR) were estimated. The following plants extracts from Acori graminei Rhizoma, Longnae Arillus, Gastrodiae Herba, Lycii Fructus, Ligusticum officinale, Ferula assafoetida, Corydalis Tuber, Eucommiae Cortex, Zizyphi spinosi Semen activated the activity of GDH I to more than 35%, and the following ones from Visci Ramulus, Ligusticum officinale, Myristicae Semen, Ferulae Resina, Scolopendrae Corpus, Corydalis Tuber, Eucommiae Cortex, Zizyphi spinosi Semen did that of GDH II. The plant extracts from Cynanchi Radix, Astragali Semen, Angelicae dahuricae Radix, Biotae orientalis Folium, Uncariae Ramulus et Uncus, Polygalae Radix, Cynomorii Herba inhibited that of GABA-T to 35% and over, and the following ones from Hyoscyamus niger, Cynanchi Radix, Acori graminei, Caesalpiniae Lignum, Cannabis Semen, Sedum aizoon, Sedum kamtschaticum, Schisandrae Fructus, Lilii Bulbus, Biotae orientalis Folium, Uncariae Ramulus et Uncus, Myristicae Semen, Akebiae Fructus, Cynomorii Herba, Buddleiae Flos, Mucunae Caulis, Zizyphi Fructus, Paeoniae Radix rubra did that of SSADH to 70% and over; the following ones from, Caesalpiniae Lignum, Sedum kamtschaticum, Schisandrae Fructus, Astragali Semen, Angelicae dahuricae Radix, Dioscorea nipponica, Myristicae Semen, Akebiae Fructus, Cynomorii Herba, Scutellariae Radix did that of SSAR.