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Quality Characteristics of Makgeolli and Nuruk Grain Inoculated with Aspergillus oryzae N041 (황국균(Aspergillus oryzae N041)을 이용한 곡류 낱알누룩 제조 및 막걸리의 품질특성)

  • Baek, Seong-Yeol;Kim, Joo-Yeon;Yun, Hye-Ju;Choi, Ji-Ho;Choi, Han-Seok;Jeong, Seok-Tae;Yeo, Soo-Hwan
    • Journal of the East Asian Society of Dietary Life
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    • v.21 no.6
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    • pp.877-881
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    • 2011
  • This study was investigated the quality characteristics of Makgeolli containing different types of Nuruk grain inoculated with A. oryzae N041. In an analysis of the quality characteristics of four types of Nuruk grain, mung beans Nuruk grain had higher acidity, amino acidity and saccharogenic power than rice Nuruk grain. In an analysis of four types of brewed Nuruk grain, mung beans Nuruk grain had the highest sugar and reducing sugar contents. On the other hand, mung beans Nuruk grain had the lowest alcohol content. Citric acid, malic acid and succinic acid have been identified as the major organic acids in Makgeolli. The amounts of organic acids were 1,748.5 mg% in rice Nuruk grain, 1,553.9 mg% in mung beans Nuruk grain, 1,003.2 mg% in barley Nuruk grain and 940.8 mg% in adlay Nuruk grain. In particular, Makgeolli brewed with mung beans Nuruk grain had the highest content of the organic acids, which is expected to deepen its flavor.

Control Effects of Frankliniella occidentalis by using Trap Plants and Orius laevigatus in Chrysanthemum PVC House (시설국화에서 트랩식물과 미끌애꽃노린재를 이용한 꽃노랑총채벌레 방제)

  • Choi, Yong-Seok;Whang, In-Su;Park, Deog-Kee;Lee, Jun-Seok;Ham, Eun-Hye
    • The Korean Journal of Pesticide Science
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    • v.17 no.4
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    • pp.440-447
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    • 2013
  • F. occidentalis (WFT, western flower thrips) is a major pest in artificial chrysanthemum houses. Nnumber of WFT attracted to yellow sticky trap was highest at trap plant and lowest at 15 and 20m places away from trap plant. Number of WFT attracted to yellow stick trap when trap plant was placed in chrysanthemum house 30 days after planting (resident WFT) was 4.4~7.7 times more than at 5 and 10 m places away from trap plant and when trap plant was placed in chrysanthemum house immediately after planting (resident WFT) was 5.7~9.4 times more at 5 and 10 m places away from trap plant. Number of WFT between the place trap plant located and unlocated was undifferent when cultured chrysanthemum formed flower. In case of the plot that trap plant and natural enemy was used simultaneously, number of WFT was highter then chemicals plot. In case of the plot that trap plant and chamicals (chemicals was sprayed on trap plant only) WFT was controlled in trap plant place only but density of WFT on 5m and 10m places away from trap plant was increased. Therefore, WFT could be controlled effectively by use of trap plant (flowering yellow chrysanthemum) and natural enemy simultaneously.

Biological control of Frankliniella occidentalis Pergande by Orius laevigatus (Fieber) and new banker plants on chrysanthomum greenhouse (시설재배 국화에서 꽃노랑총채벌레 방제를 위한 미끌애꽃노린재와 new banker plants의 혼합 적용 효과)

  • Ham, Eun Hye;Park, Jong Kyun;Choi, Yong Seok;Lee, Jun Seok;Choi, Young Chul
    • Journal of Sericultural and Entomological Science
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    • v.52 no.1
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    • pp.33-38
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    • 2014
  • This studies by treatment of 25 individual adults of O. laevigatus and Banker plant with different number of eggs of O. laevigatus were conducted to develop new biological control of the western flower thrips (WFT) in chrysanthemum house. In this work, a treatment of 25 individual adults of O. laevigatus, Banker plant with different number of eggs of O. laevigatus and mixed treatment were applied. Initial WFT density per plant were 5 individuals. WFT density was measured every 7days after treatment for 7 weeks. Biological control of WFT by 25 individual adults of O. laevigatus and Banker plant with 25 eggs of O. laevigatus were applied 1 time and resulted in the high control value of thrips 91.8%. The population of WFT was kept under the economic threshold level (1 individual per plant). For the last three years we developed new banker plant system. This system is being developed to improve biological control of WFT on chrysanthemums.

Effects of Gamma Irradiation on Queso Blanco Cheese (퀘소블랑코 치즈의 감마선 조사 처리 효과)

  • Jeong, Seok-Geun;Noh, Young-Bae;Shin, Ji-Hye;Han, Gi-Sung;Chae, Hyun-Seok;Yoo, Young-Mo;Ahn, Jong-Nam;Lee, Ju-Woon;Jo, Cheor-Un;Lee, Wan-Kyu;Ham, Jun-Sang
    • Journal of Dairy Science and Biotechnology
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    • v.25 no.1
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    • pp.15-20
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    • 2007
  • Effects of gamma irradiation on chemical, microbiological, and immunological changes of Queso Blanco cheese were investigated. Although Queso Blanco cheese was made by heat pasteurization at 85$^{\circ}$C and addition of acid without lactic starter culture, total bacterial counts and lactic acid bacterial counts of control cheese were 7.65${\pm}$0.04 and 7.64${\pm}$0.02 log CFU/mL, respectively. It was thought that this microbial growth was due to the incomplete inactivation of raw milk by the heat treatment, resulting into growth during the pressing and the drying process. It demonstrated the possibility that if heat- and acid-resistant hazard microbes are present in raw milk, they can grow during the processes. Lactic acid bacterial counts of the irradiated cheese were 5.45${\pm}$0.02 log CFU/mL at 1kGy, 2.12${\pm}$0.12 log CFU/mL at 2kGy, and not detected at 3kGy or higher doses. The reduction of antigenicity by gamma irradiation was not found. It might be caused by the fact that most whey proteins of milk, a major antigen in milk, were already denaturated by heat process and removed during the draining.

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Melanocyte-stimulating Hormone Receptor (MC1R) Genotype and Its Effects on Coat Color in Korean Jindo Dogs

  • Hong, Kyung-Won;Kim, Sang-Wook;Jang, Hong-Chul;Yang, Seung-Min;Shin, Young-Bin;Hong, Yoon-Hye;Kim, Jong-Seok;Oh, Seok-Il;Choi, Yoon-Ju;Chung, Dong-Hee;Yang, Boh-Suk;Lee, Ji-Woong;Choi, Bong-Hwan
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.8
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    • pp.1078-1084
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    • 2009
  • The Jindo dog is a Korean natural monument and is recognized by the Fédération Cynologique Internationale. A prominent feature is the diverse coat color within the breed. To analyze the genetic basis of variation in the Jindo coat color, we sequenced the protein-coding regions of the melanocortin 1 receptor gene (MC1R). The MC1R coding sequence was determined from 154 dogs in five breeds (Jindo, Labrador Retriever, English Springer Spaniel, Belgian Malinois, and German Shepherd). To confirm the genetic structure of sampled populations, we tested for Hardy-Weinberg equilibrium (HWE) and computed $F_{st}$ The sample populations did not significantly deviate from HWE. $F_{st}$ was 0.02 between white and fawn Jindo dogs; this was lower than $F_{st}$ between breeds. Six single nucleotide polymorphisms (SNPs) were detected in the MC1R coding region. Among the six SNPs, five were non-synonymous (S90G, T105A, Q159P, M264V, and R306ter) and one was synonymous SNP (Y298Y). From the SNPs, we predicted four haplotypes (H1, H2, H3, and H4) for Jindo MC1R. Jindo dogs had different haplotypes corresponding to different coat colors. H1 was frequently observed in white Jindo dogs with an odds ratio of 5.03 (95% CI: 2.27-11.18, p<0.0001), whereas H2 and H4 were observed only in fawn Jindo dogs. Our findings indicate that SNP haplotype can influence coat color. Knowledge of MC1R haplotypes can help discriminate white and fawn coats in Jindo dogs. We hope this report will trigger more research into the genetics of this traditional Korean dog and will be a reference for dogs of Asian origin. Also, our results will provide a useful genetic marker for Jindo dog breeders who have selected for specific colors.

Evaluation and Assessment of Biosafety for Bacillus thuringiensis (Bt)-transgenic Rice: Responses of Daphnia magna Fed on Bt-transgenic Rice Variety (해충저항성 Bacillus thuringiensis (Bt) 벼의 환경위해성 평가: 해충저항성 Bt벼가 물벼룩(Daphnia magna)에 미치는 영향)

  • Oh, Sung-Dug;Shin, Hye-Chul;Sohn, Soo-In;Lee, Ki-Jong;Kim, Hyo-Jin;Ryu, Tae-Hun;Lee, Jang-Yong;Park, Beom-Seok;Kweon, Soon-Jong;Suh, Seok-Cheol;Park, Jong-Sug
    • Journal of Applied Biological Chemistry
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    • v.54 no.4
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    • pp.296-302
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    • 2011
  • Insect-resistant transgenic rice was developed by inserting the mCry1Ac1 a modified gene from the soil bacterium Bacillus thuringiensis (Bt). For biosafety assessment, we studied the effects on survival of cantor Daphnia magna, a commonly used as a model organism in ecotoxicological studies. D. magna fed on Bt rice and its near non-genetically modified (GM) counterparts (Nakdong) grown in the same environment (100% ground rice suspension). The Bt rice was comfirmed to have the insertion of T-DNA and protein expression by the polymerase chain reaction and ELISA analysis. Feeding study showed similar cumulative immobility and abnormal response of D. magna between Bt rice and non-GM counterparts. 48 h-$EC_{50}$ values of Bt rice and non-GM rice showed 4,429 and 2,889 mg/L respectively. The rice no observed effect concentration (NOEC) values for D. magna was suggested 1,000 mg/L. We conclude that the tested Bt-rice and Nakdong similar cumulative immobility for D. magna the widely used model organism. We found out that there is strong possibility that the growth of Bt rice didn't affect to non-target insects.

Production of $Makgeolli$ Using Rice Treated with $Gaeryang-Nuruk$ (for Non-steaming Process) Extract (시판 무증자용 개량누룩 추출물로 처리한 쌀을 이용한 막걸리 제조)

  • Park, Ji-Hye;Yeo, Soo-Hwan;Choi, Ji-Ho;Jeong, Seok-Tae;Choi, Han-Seok
    • Food Science and Preservation
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    • v.19 no.1
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    • pp.144-152
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    • 2012
  • The temperature changes and quality characteristics of $Makgeolli$ produced using rice treated with $Gaeryang-Nuruk$ (commercial improved $Nuruk$) extract were investigated. During fermentation, the treated rice maintained a lower temperature than the control and then rose after the fifth mashing day. For all the treatments, the numbers of yeast, LAB and AAB colonies increased on the second mashing day, and then gradually decreased. As the fermentation proceeded, the pH gradually increased from the third mashing day, and eventually became higher than that on the initial mashing day. The total acid contents increased on the first mashing day, but as the fermentation progressed, they showed little change. The amino acidity and soluble solid contents during overall fermentation and reducing-sugar contents was reduced until the first mashing day, and increased from the day after. As for the alcohol content, that of the control was 6.87% on the first mashing day, and then gradually increased, nuruk-extract-treated rice began with 9~10% alcohol content and then increased as the fermentation proceeded. Among the organic acid contents, lactate was the main material. In the sensory evaluation, the 24 h-, 48 h- and 72 h- treated rice samples showed somewhat good response.

A Study on the Quality Characteristics of Makgeolli Using Heat Treatment of Traditional Korean Nuruk Extract (재래누룩 추출물을 열처리한 막걸리의 품질 특성)

  • Park, Ji-Hye;Choi, Ji-Ho;Yeo, Su-Hwan;Jeong, Seok-Tae;Choi, Han-Seok;Kang, Ji-Eun;Kim, So-Ra
    • Journal of the East Asian Society of Dietary Life
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    • v.23 no.5
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    • pp.620-628
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    • 2013
  • In this study, the condition for preventing abnormal fermentation was set by heating the nuruk extract, such that glycosyl enzymes maintain its activity and unnecessary microbes are removed. The total colony of microbes in the heated nuruk extract was highest in number at $25^{\circ}C$ and began to reduce at 50, $60^{\circ}C$ and sharply reduced over $70^{\circ}C$. Saccharogenic power (SP), glucoamylase and acidic protease activities were highest at $50^{\circ}C$, 10, 20, 30min and ${\alpha}$-amylase activity was lower at $50^{\circ}C$ than at $25^{\circ}C$. In the pHs of the nuruk extract, as the heat temperature became higher and treatment time was longer, the pHs were reduced significantly. The total acidities of heat treatments at 50, $60^{\circ}C$ were lower by 0.2% than at $25^{\circ}C$, where as the 70, $80^{\circ}C$ treatments showed a sharp rise from the early stage of fermentation. Soluble solids showed the same aspects with the glycosyl enzymes cases. In reducing sugar, 25, $50^{\circ}C$ treatments were sharply increased from the first day of fermentation while $60^{\circ}C$ treatments began to rise from second day 70, $80^{\circ}C$ were slightly increased after the fourth day. The normally alcohol fermented treatments were 25, 50 and $60^{\circ}C$ 30min. The $70^{\circ}C$ treatments almost did not alcohol fermentation. In the preference tests, taste and total acceptability were high at 25, $50^{\circ}C$ treatments. These results suggest that makgeolli using heat treated nuruk extract also has good taste as well as did not.

A Case of Hypersensitivity Pneumonitis Following Placenta Extract Injection (태반 추출물 주사 후 발생한 과민성 폐장염 1예)

  • Lee, Sang Seok;Choo, Young Kwang;Bang, Chang Seok;Kim, Youn Seup;Park, Jae Seuk;Jee, Young Koo;Kim, Doh Hyung;Myong, Na Hye
    • Tuberculosis and Respiratory Diseases
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    • v.66 no.6
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    • pp.471-476
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    • 2009
  • Human placenta contains various kinds of nutritional elements essential for embryonic development. Currently, human placenta extracts are widely overused in Korea to improve certain health conditions (postmenopausal syndrome, liver function, and cosmetic purposes) without scientific evidence that they actually work. The use of placenta extracts should be restricted, due to a lack of systematic research on the therapeutic effectiveness and adverse results from these treatments. While the common adverse effects that have been reported are fever, rash, itching, nausea, vomiting, breast pain, and rare cases of anaphylactic shock, there have been no reports of pulmonary complications such as hypersensitivity pneumonitis. Recently, we experienced a patient with hypersensitivity pneumonitis following a placenta extract injection. To our knowledge, this is the first case of hypersensitivity pneumonitis associated with placenta extract use.

Clinical Study of Blood Flow Velocity Using Transcranial Doppler Ultrasound on Cerebral Arteries in Dizziness Patients (경두개 도플러 초음파를 이용한 현훈 환자의 뇌혈관 혈류 측정에 관한 임상적 연구 (중대뇌동맥, 추골동맥 및 기저동맥을 중심으로))

  • Kim, Bong-Suk;Oh, Jung-Han;Kim, Dong-Woo;Choi, Bin-Hye;Jang, Woo-Seok;Seo, Young-Ho;Son, Dae-Yong;Byun, Joon-Seok;Lim, Hee-Yong
    • The Journal of Internal Korean Medicine
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    • v.25 no.3
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    • pp.418-426
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    • 2004
  • Objectives : This study was designed to research whether transcranial doppler ultrasound(TCD) can yield a suitable diagnosis or prognosis for dizziness. Methods : To evaluate the blood flow, we measured the mean velocity(Vm), peak systolic velocity(Vs) and pulsatility index(PI) of the middle cerebral artery(MCA), vertebral artery(VA) and basilar artery(BA) in 54 dizziness patients and 44 normal subjects. To investigate the difference between dizziness patients and normal subjects, we selected 54 dizziness patients diagnosed normal by Brain MRI, and 44 normal subjects who did not have any symptoms of hyperlipemia, hypertension, diabetes melitus, heart disease, or smoking, diagnosed normal by Brain MRI. 30-69 year olds were divided into 30-49 year olds, 50-59 year olds and 60-69 year olds. Results : In normal subjects, there was a significantly higher Vm, Vs in the MCA and BA, and Vs in the VA of female subjects, and there was a significant difference in PI in the MCA and BA increasing with age. In comparison with normal subjects, dizziness patients had a significantly lower Vm in the BA. Male dizziness patients had significantly lower PI in the MCA. 30-49 year olds had significantly lower Vm in the BA. Conclusions : The significant difference in blood flow velocities in the BA between dizziness patients and normal subjects suggests that dizziness is related to blocking of blood flow in the BA and that diagnosis and prognosis can be made through TCD. More detailed study of dizziness patients will be needed.

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