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The Quality Characteristics of Commercial Deonjang Certified for Traditional Foods (전통식품 품질인증 된장의 품질특성)

  • Kang, Ji-Eun;Choi, Hye-Sun;Choi, Han-Seok;Park, Shin-Young;Song, Jin;Choi, Ji-Ho;Yeo, Su-Hwan;Jung, Seok-Tae
    • The Korean Journal of Community Living Science
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    • v.24 no.4
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    • pp.537-542
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    • 2013
  • Deonjang has been developed as a fermented food in Korea. It produces a distinctive flavors and tastes during the fermentation process. The purpose of this study was to determine the quality characteristics of commercial deonjang certified for traditional foods. We investigated the amino nitrogen, sodium chloride(NaCl), total colony counts, coliforms, Bacillus cereus and isoflavone of 24 commercial deonjang samples certified for traditioinal foods. Deonjang showed wide ranges in amino nitrogen(105.76~318.93 mg%) and NaCl(12.53~16.51%). Survey distribution of microflora investigation in the total colony counts were detected in all 24 samples(100%), and the range is low $1.5{\times}10^6$ CFU/g, at the highest $2.5{\times}10^9$ CFU/g respectively. For the coliform, the following results were $0{\sim}5.0{\times}10^1$ CFU/g. B.cereus was detected in a total of four samples were $2.5{\times}10^3{\sim}3.3{\times}10^4$ CFU/g in the distribution. Daidzein of isoflavones showed the lowest at 86.7 ppm, 681.8 ppm range of the best shows and genistein as low as 0 to 50.0 ppm respectively. This research provided information for quality characteristics of commercial deonjang certified for traditional foods.

Concentrations and Risk Assessment of Total Mercury and Methyl Mercury in Commercial Marine Fisheries from Korea (한국산 수산물의 총 수은 및 메틸수은 농도 및 위해도 평가)

  • Choi, Minkyu;Yun, Sera;Park, Hye-Jung;Lee, Ja-Yeon;Lee, In-Seok;Hwang, Dong-Woon;Yoon, Min-Cheol;Choi, Woo Seok
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.6
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    • pp.675-683
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    • 2017
  • Total mercury (T-Hg) and methyl mercury (MeHg) were determined in marine fisheries (41 species, n=87) commonly consumed in Korea, using a gold amalgamation method and gas chromatography-cold vapor atomic fluorescent spectroscopy, respectively. Concentrations of T-Hg and MeHg in all samples (31 fish, 4 crustaceans, 4 cephalopods, and 2 gastropod species) were in the range of 0.016-0.495 (mean, 0.093) mg/kg-wet and not detected-0.338 (mean, 0.067) mg/kg-wet, respectively. The concentrations of MeHg in marine fisheries were significantly correlated with T-Hg concentrations (P<0.001). The highest mean concentrations of T-Hg and MeHg were found in fish species, followed by crustaceans. The contribution of MeHg to T-Hg was in the range of 64-95% (mean, 83%) in cephalopods, 28-98% (mean, 69%) in fish, and 26-88% (mean, 57%) in crustaceans. The weekly intakes of T-Hg and MeHg by fisheries consumption for the Korean general population were estimated to be 0.463 and $0.338{\mu}g/kg$ body weight/week, respectively. The concentrations and intakes of T-Hg and MeHg were less than the allowable residue levels and in the range of 12 to 17% of the provisional tolerable weekly intake (PTWI) applied in Korea.

Multiple Densities of the Chronic Subdural Hematoma in CT Scans

  • Park, Hye-Ran;Lee, Kyeong-Seok;Shim, Jae-Jun;Yoon, Seok-Mann;Bae, Hack-Gun;Doh, Jae-Won
    • Journal of Korean Neurosurgical Society
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    • v.54 no.1
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    • pp.38-41
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    • 2013
  • Objective : Density of the chronic subdural hematoma (cSDH) is variable. It often appears to be mixed density. Multiple densities of cSDH may result from multiple episodes of trauma. We investigated the frequency of mixed density and the causes of head injuries representing each density. Methods : We could collect 242 cases of chronic SDH. The cSDHs were classified into four groups; hypodensity, homogeneous isodensity, layered type, and mixed type on the basis of CT scans. Results : The density of cSDH was isodense in 115 patients, hypodense in 31 patients, mixed in 79 cases, and layered in 17 cases. The cSDH was on the left side in 115 patients, on the right side in 70 patients, and bilateral in 40 patients. The history of trauma was identifiable in 122 patients. The etiology could be identified in 67.7% of the hypodense hematomas, while it was obscure in 59.5% of the mixed hematomas. Conclusion : Mixed density of cSDH results from multiple episodes of trauma, usually in the aged. It is hard to remember all the trivial traumas for the patients with the mixed density cSDHs. Although there were membranes within the mixed density hematomas, burr-holes were usually enough to drain the hematomas.

Optical Gap Bowing and Phonon Modes of Amorphous Ge1-x-ySexAsy Thin Films

  • So, Hyeon-Seop;Park, Jun-U;Jeong, Dae-Ho;Lee, Ho-Seon;Sin, Hye-Yeong;Yun, Seok-Hyeon;An, Hyeong-U;Kim, Su-Dong;Lee, Su-Yeon;Jeong, Du-Seok;Jeong, Byeong-Gi
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.288.1-288.1
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    • 2014
  • We investigated the optical properties of Ge1-xSex and Ge1-x-ySexAsy amorphous semiconductor films using spectroscopic ellipsometry and Raman spectroscopy. The dielectric functions and absorption coefficients of the amorphous films were determined from the measured ellipsometric angles. We obtained the optical gap energies and Urbach energies from the absorption coefficients, and found a strong bowing effect in the optical gap energy of Ge1-x-ySexAsy where the endpoint binaries were Ge0.50Se0.50 and Ge0.31As0.69. Based on the correlation between optical gap energies and Urbach energies, the large bowing parameter was attributed to the electronic disorder. We found the composition dependence of several phonon modes using Raman spectroscopy. For Ge1-x-ySexAsy, the D mode (232-267 cm-1) changed from As-As (or As3 pyramid), to As(Se1/2)3 pyramid, and finally to Se clusters, as the Se composition increased. Resonant Raman phenomenon was observed in Ge0.38Se0.62 at a laser excitation of 514 nm (2.41 eV). We verified that this laser energy corresponds to the transition energy of Ge0.38Se0.62 using the second derivative of the dielectric function of Ge0.38Se0.62.

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The Utility of Liver Transaminase as a Predictor of Liver Injury in Blunt Abdominal Trauma (복부 둔상 환자에게 간 손상 예측을 위한 Liver Transaminase의 유용성)

  • Lee, Jong-Seok;Oh, Sung-Chan;Kim, Hye-Jin;Cho, Suk-Jin;Lee, Sang-Lae;Ryu, Seok-Yong
    • Journal of Trauma and Injury
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    • v.23 no.2
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    • pp.151-156
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    • 2010
  • Purpose: The liver is the second most common organ injured by blunt abdominal trauma. The purpose of this study was to determine the utility of liver transaminase in screening blunt abdominal trauma patients for traumatic liver injury. Methods: We retrospectively reviewed the medical records of 231 patients who sustained blunt trauma and were at risk for traumatic liver injury between June 2009 and August 2010. All of them underwent a focused assessment with sonography for trauma (FAST) and abdominal computed tomography (CT). Based on the diagnosis of abdominal CT, patients were divided into two groups: group I with liver injury and group II without liver injury. We compared the two groups and calculated the sensitivity, the specificity and the predictive values of serum aspartate aminotransferase (AST) and serum alanine aminotransferase (ALT) by using multiple cutoff values. Results: Of 231 patients with no abdominal free fluid in the FAST, 33 had traumatic liver injury on abdominal CT. The mean AST and ALT levels in group I (311.6 IU/L and 228.1 IU/L, respectively) were significantly higher than the values in group II (48.4 IU/L and 35.6 IU/L, respectively). The cutoff to distinguish liver injury is 60 IU/L for AST and 58 IU/L for ALT, with 93.8% sensitivity and 79.8% specificity for AST, and 90.6% sensitivity and 87.4% specificity for ALT. Conclusion: We recommend that all patient with suspected blunt abdominal trauma be evaluated using serum liver transaminase as a screening test for liver injury even though no abdominal free fluid is shown on the FAST. If AST > 60 IU/L and/or ALT > 58 IU/L, abdominal CT was useful to confirm liver injury in this study.

Association between Prostaglandin-endoperoxide Synthase 2 (PTGS2) Polymorphisms and Blood Pressure in Korean Population

  • Jin, Hyun-Seok;Hong, Kyung-Won;Lim, Ji-Eun;Han, Hye-Ree;Lee, Jong-Young;Park, Hun-Kuk;Oh, Berm-Seok
    • Genomics & Informatics
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    • v.6 no.3
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    • pp.110-116
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    • 2008
  • Blood pressure refers to the force exerted by circulating blood on the walls of blood vessels, and chronical elevation of blood pressure is known as hypertension. Although hypertension is affected by genetic and environmental factors, the genetic background of hypertension is not fully understood. One of the candidate genetic factors, Prostaglandin-endoperoxide synthase 2 (PTGS2), is a membrane-bound enzyme, catalyzing the conversion of arachidonic acid to prostaglandin, and recently SNPs of PTGS2 gene was associated with hypertension in Japanese population. Therefore the association of PTGS2 polymorphisms was investigated with blood pressure in healthy Korean subjects, 470 unrelated individuals randomly selected from Ansung and Ansan cohorts. The 25 SNPs of PTGS2 gene were identified by the sequencing analysis of 24 Korean samples. Among identified polymorphisms, three SNPs (rs689466, -1329A>G; rs5275, +6365T>C; rs4648308, +8806G> A) were selected for further association analysis, and rs689466 located in promoter region was associated with blood pressure as well as triglyceride level in the blood. By in silico analysis, rs689466 locates in v-Myb transcription factor binding site, and the v-Myb site disappears when the SNP is changed from A to G nucleotide. Individuals with A/G and G/G genotype in rs689466 have higher blood pressure than those with A/A genotype, and the regression p-value is 0.008 for systolic and 0.004 for diastolic blood pressure. In summary, the PTGS2 polymorphism (rs689466) is associated with blood pressure in Asian populations based on this and Japanese studies, shedding light on it as a genetic risk marker of hypertension.

Farnesylcysteine Methyltransferase Activity and Ras Protein Expression in Human Stomach Tumor Tissue

  • Han, Eui-Sik;Oh, Hye-Young;Ha, Kwang-Won;Han, Beom-Seok;Hong, Seok-Min;Han, Jung-Whwan;Hong, Sung-Youl;Noh, Sung-Hun;Lee, Hyang-Woo
    • Archives of Pharmacal Research
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    • v.21 no.4
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    • pp.378-384
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    • 1998
  • The processing pathway of G-proteins and Ras family proteins includes the isoprenylation of the cysteine residue, followed by proteolysis of three terminal residues and .alpha.-carboxyl methyl esterification of the cysteine residue. Farnesylcysteine methyltransferase (FCMT) activity is responsible for the methylation reaction which play a role in the membrane attachment of a variety of cellular proteins. Four kinds of Ras protein (c-Ha-ras, c-N-Ras, c-Ki-Ras, pan-Ras) expression were detected in adenocarcinoma of human tissue by immunohistochemical method, and hematoxylin and eosin staining. The level of Ras protein in human stomach tumor tissues was much higher than in normal and peritumoral regions of the same biopsy samples. The FCMT activities of each cellular fractions were high in mitochondrial fraction followed by microsomal fraction, whole homogenate and cytosolic fraction. The inhibitory effect on FCMT activity on stomach tumor tissue was determined after treatment with 0.25 $\mu\textrm{M}$ of S-adenosyl-$_L$-homocysteine. S-adenosyl-$_L$-homocysteine inhibited FCMT activity from 11.2% to 30.5%. These results suggested that FCMT might be involved in Ras proteins activity.

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Effect of Milk Containing Streptococcus thermophilus KACC 91147 on Blood Glucose Levels (Streptococcus thermophilus KACC 91147 첨가우유 섭취가 혈당치에 미치는 영향)

  • Ham, Jun-Sang;Jeong, Seok-Geun;Noh, Young-Bae;Shin, Ji-Hye;Han, Gi-Sung;Chae, Hyun-Seok;Yoo, Young-Mo;Ahn, Jong-Nam;Cho, Yong-Min;Kim, Geun-Bae
    • Food Science of Animal Resources
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    • v.27 no.4
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    • pp.496-500
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    • 2007
  • The lactase activities of nine species of lactic acid bacteria were compared using the chromogenic substrate, $o-nitrophenyl-{\beta}-D-galactopyranoside$. Streptococcus thermophilus KACC 91147 had the highest lactase activity among a total of thirty strains of Lactobacillus and S. thermophilus tested, including commercial strains. S. thermophilus KACC 91147 released $0.30{\pm}0.12\;mg/mL$ of galactose in treated milk A ($10^7\;CFU/mL$) and $6.49{\pm}0.38\;mg/mL$ in treated milk B ($10^9\;CFU/mL$ milk) over 2 hours. In milk tolerance tests, the blood glucose level (BGL) of 6 volunteers (2 males and 4 females) clinically diagnosed as lactose intolerant increased 3.0 mg/dl after drinking milk A, but a significant (p<0.05) additional increase of $11.2{\pm}4.18\;mg/dl$ was found after drinking milk B. This result suggests that the addition of S. thermophilus KACC 91147 cells into milk aids the digestion of lactose in milk and ameliorates the symptoms of lactose-intolerant individuals due to the activity of lactase from the lactic streptococci.

Determination of Optimum Conditions for Mass Rearing of Cadra cautella(Walker)(Lepidoptera: Phycitidae) and Orius laevigatus(Fieber)(Hemiptera: Anthocoridae) (줄알락명나방(Cadra cautella)과 미끌애꽃노린재(Tyrophagus putrescentiae)의 증식 최적화 조건 탐색)

  • Ham, Eun Hye;Choi, Yong Seok;Lee, Jun Seok;Park, Jong Kyun
    • Journal of Sericultural and Entomological Science
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    • v.50 no.2
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    • pp.112-115
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    • 2012
  • To develop an efficient mass rearing system for Cadra cautella(Walker) and Orius laevigatus(Fieber) were investigated under the conditions of photoperiod 16L : 8D, $70{\pm}5%$ RH and $23{\pm}2^{\circ}C$. The highest efficiency rate(multiply 28.6) of C. cautella was achieved when using the rearing cage of $20{\ell}$ than $3{\ell}$. The highest efficiency rate(multiply 16.3) of O. laevigatus was achieved when using the rearing cage of $5{\ell}$ (adult 1,500, 10 pcs(10 cm) of plant of egg taking). The moth could be successfully reared with all food substrates tested, of which rice bran(43%) + chick feed(43%) + yeast(14%) assumed to be proper for mass-rearing in view of cost.

Effects of Dissolved Inorganic and Organic Nutrient (Nitrogen and Phosphorus) on the Growth of Dinoflagellate Alexandrium affine (와편모조류 Alexandrium affine의 생장에 미치는 용존태 무기 및 유기 영양염(질소와 인)의 영향)

  • Oh, Seok Jin;Kim, Ji Hye;Park, Kyung Woo;Kim, Seok-Yun
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.27 no.5
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    • pp.630-638
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    • 2021
  • In this study, we investigated the effects of dissolved inorganic and organic nutrient on the growth of dinoflagellate Alexandrium affine (LIMS-PS-2345). The maximum uptake rates (ρmax) and half saturation constants (Ks) calculated from the uptake kinetics experiment were 77.0 pmol/cell/hr, 17.6 μM for nitrate and 15.5 pmol/cell/hr, 3.88 μM for phosphate, respectively. These results suggested that this species has high inorganic nutrient demand and a low affinity for inorganic nutrients. During the utilization of organic nutrients for A. affine, growth rates of experimental groups added by organic nitrogen (urea and glycine) and phosphorus (adenosine triphosphate and glycerol phosphate) were above 70 %, compared to the experimental groups added by inorganic nutrients. Thus, A. affine may need to utilize organic nutrients to understand the dominant strategy and advantageous position in the interspecific competition within low inorganic nutrient environments.