• Title/Summary/Keyword: Hye-seok

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Characteristics and Pathogenicity of Host Range Expanded Recombinant Viruses in Insect Cells (숙주범위가 넓어진 재조합 바이러스의 세포주에서의 특성 및 병원성)

  • Kim, Hye-Sung;Woo, Soo-Dong;Kim, Woo-Jin;Choi, Jae-Young;Jin, Byung-Rae;Lee, Youn-Hyung;Kang, Seok-Kwon
    • The Journal of Korean Society of Virology
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    • v.27 no.1
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    • pp.29-37
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    • 1997
  • To use recombinant viruses with wider host range as viral insecticides, we investigated the characteristics and pathogenicity of host range expanded recombinant viruses in insect cells. We compared host range expanded recombinant viruses, RecS-B6 and RecB-8, constructed by cotransfection of Autographa californica nuclear polyhedrosis virus (AcNPV) and Bombyx mori NPV (BmNPV), to host range expanded AcNPV, Ac-BH, by substitution of the 0.6 Kb fragment of the BmNPV helicase gene. Restriction endonuclease profiles of RecS-B6 and RecB-8 DNAs were different from those of parent viruses. Nucleotide sequence analysis of the 0.6 Kb region in the putative helicase gene of RecS-B6 and RecB-8 showed that their structures were identical to the counterpart region of BmNPV. Comparison of viral replication of these recombinant viruses in Sf-21 and BmN-4 cells showed that Ac-BH, compared to wild type viruses, replicated well in BmN-4 cells but poorly in Sf-21 cells. In contrast, RecS-B6 and RecB-8 replicated relatively well in both cells compared to parent viruses. These results may imply that random genomic recombinant viruses, RecS-B6 and RecB-8, possess better potential as viral pesticides than helicase-mediated recombinant virus, Ac-BH.

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In vitro induction of hairy root from isoflavones-producing Korean wild arrowroot Pueraria lobata

  • Kim, Soo-Jung;Cha, Min-Seok;Lee, Eun-Ji;Kim, In-Hye;Kwon, Jung-Eun;Kang, Se-Chan;Park, Tae-Ho
    • Journal of Plant Biotechnology
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    • v.39 no.3
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    • pp.205-211
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    • 2012
  • Pueraria lobata is a perennial legume plant, widely distributed in the countries of East Asia. It is a medicinally important leguminous plant and produces various isoflavones such as puerarin, daidzein etc which have potential for preventing several chronic diseases including osteoporosis, cardiovascular disease and cancer. In this study, we tried to induce hairy roots in vitro from Korean wild arrowroot P. lobata and investigated the effects of hormones and light conditions. Initially leaf and stem segments were infected with Agrobacterium rhizogenes and incubated in different conditions. Hairy roots were induced from only stem segments and the induction was best at dark condition and the presence of IBA during incubation. Secondary roots were also significantly much more induced at the dark condition than at the 16 hours light condition. Among plant growth regulators of auxin, IBA was best for secondary root formation while 2,4-D, IAA and NAA produced callus or less hairy roots. The presence of the foreign gene rolC transferred by A. rhizogenes that plays a major role in hairy root induction was confirmed by PCR. The accumulation of isoflavones such as puerarin and daidzin was also confirmed. These results will facilitate mass production of hairy root and can be used for the production of functional substances from wild arrowroots.

Environmental Change and Policy of Solar Photovoltaic Industry (태양광 산업의 환경 변화와 정책)

  • Choi, Hyukjoon;Kim, Minji;Kim, Haeyeon;Yun, Ga-Hye;Lim, Seok Ki
    • 한국신재생에너지학회:학술대회논문집
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    • 2011.11a
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    • pp.153-153
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    • 2011
  • 신 재생에너지 정책이 발전차액지원제도(Feed In Tariff; FIT)에서 신 재생에너지 의무할당제(Renewable Portfolio Standard; RPS)로 변화하면서 원별 경쟁이 가속화되고 있다. 태양광 산업 역시 이러한 환경 변화에 대처해야 하며, 그를 위한 정책 구성이 필요하다. 이에 태양광 산업 정책은 크게 두 부분으로 구분하여 진행해야 한다. 첫째, 폴리실리콘(Poly silicon)을 활용하는 다결정실리콘 태양광에너지에 초점을 맞춘 정책이며, 둘째, 미래에 상용화될 차세대 태양광 에너지에 대한 대비를 위한 연구 개발(R&D) 정책이다. 먼저 다결정실리콘 태양광에너지에 초점에서의 산업 정책은 산업육성정책과 수출정책, 인프라 구성 등으로 나눌 수 있다. 현재 과도한 국가부채로 인한 세계경제 악화로 태양광에너지 업체들의 경제성이 악화하고 있다. 더욱 빨리 그리드 패리티(Grid Parity)를 달성하기 위해 수직통합 등의 필요성이 대두하고 있다. 이에 본 연구는 그리드 패리티 달성시기를 위해 태양광 산업 내 세대 변화를 하는 경우와 하지 않는 경우를 비교하기 위해 고려할 요소를 분석하였다. 현재 신 재생에너지 가운데 태양광에너지는 타에너지원 대비 가격경쟁력을 갖추지 못한 상황이다. 그러나 수출을 고려했을 때의 향후 한국의 차세대 성장동력으로의 발전가능성이 존재한다. 따라서 가격경쟁력이 가장 중요한 영향을 미칠 신 재생에너지 의무할당제 정책 하에서 태양광에너지가 전혀 채택되지 않는 상황을 막기 위한 정책이 필요하다. 그를 위해 필요한 정책적 요소들을 알아보았다. 마지막으로 인프라 구성을 위해 태양광 산업의 가치사슬(Value Chain) 상에서의 기업 분포와 경쟁력에 대한 조사를 시행하였다. 이는 태양광 산업 내의 경쟁력을 갖춘 부문과 그렇지 못한 부문을 구별하기 위함이다. 미래에 상용화될 차세대 태양광 에너지를 준비하는 과정에서는 연구개발 관련 정책이 가장 중요하게 다뤄야 할 부분이며, 그를 위해 정부 차원에서 지원하고자 하는 기술로드맵 등에 대해서 정리하였다.

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Key Factors for the Development of Silicon Quantum Dot Solar Cell

  • Kim, Gyeong-Jung;Park, Jae-Hui;Hong, Seung-Hwi;Choe, Seok-Ho;Hwang, Hye-Hyeon;Jang, Jong-Sik
    • Proceedings of the Korean Vacuum Society Conference
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    • 2012.02a
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    • pp.207-207
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    • 2012
  • Si quantum dot (QD) imbedded in a $SiO_2$ matrix is a promising material for the next generation optoelectronic devices, such as solar cells and light emission diodes (LEDs). However, low conductivity of the Si quantum dot layer is a great hindrance for the performance of the Si QD-based optoelectronic devices. The effective doping of the Si QDs by semiconducting elements is one of the most important factors for the improvement of conductivity. High dielectric constant of the matrix material $SiO_2$ is an additional source of the low conductivity. Active doping of B was observed in nanometer silicon layers confined in $SiO_2$ layers by secondary ion mass spectrometry (SIMS) depth profiling analysis and confirmed by Hall effect measurements. The uniformly distributed boron atoms in the B-doped silicon layers of $[SiO_2(8nm)/B-doped\;Si(10nm)]_5$ films turned out to be segregated into the $Si/SiO_2$ interfaces and the Si bulk, forming a distinct bimodal distribution by annealing at high temperature. B atoms in the Si layers were found to preferentially substitute inactive three-fold Si atoms in the grain boundaries and then substitute the four-fold Si atoms to achieve electrically active doping. As a result, active doping of B is initiated at high doping concentrations above $1.1{\times}10^{20}atoms/cm^3$ and high active doping of $3{\times}10^{20}atoms/cm^3$ could be achieved. The active doping in ultra-thin Si layers were implemented to silicon quantum dots (QDs) to realize a Si QD solar cell. A high energy conversion efficiency of 13.4% was realized from a p-type Si QD solar cell with B concentration of $4{\times}1^{20}atoms/cm^3$. We will present the diffusion behaviors of the various dopants in silicon nanostructures and the performance of the Si quantum dot solar cell with the optimized structures.

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Status of ITU-T International Standard Development on Rare Metal Recycling (희소금속 재활용을 위한 ITU-T 국제표준 개발현황)

  • Lee, Mi Hye;Choi, Won Jung;Seo, Seok-Jun;Kim, Bum Sung
    • Journal of Powder Materials
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    • v.23 no.4
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    • pp.325-330
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    • 2016
  • Owing to increasing demand of rare metals present in ICT products, it is necessary to promote the rare metal recycling industry from an environmental viewpoint and to prevent climate change. Despite the fact that information for toxic substances is partly indicated, a legal basis and an international standard indicating usage of rare metals is insufficient. In order to address this issue, a newly created study group of environment and climate change at the ITU (International Telecommunication Union) is doing research to develop methodologies for recycling rare metals from ICT products in an eco-friendly way. Under this group, the Republic of Korea has established two international standards related to rare metals present in ICT products. The first is 'Release of rare metal information for ICT products (ITU-T L.1100)' and the other is 'Quantitative and qualitative analysis methods for rare metals (ITU-T L.1101)'. A new proposal for recommending the provision of rare metal information through a label by manufacturers and consumer/recycling businesses has been approved recently and is supposed to be published later in 2016. Moreover, these recommendations are also being extended to IEC, ISO and other standardization organizations and a strategy to reinforce the ability for domestic standardization is being established in accordance with industrial requirements. This will promote efficient recycling of rare metals from ICT products and will help improve the domestic supply of rare metals.

Species Composition and Community Structure of Castanopsis cuspidata var. thunbergii Communities (모밀잣밤나무군락의 종 조성 및 군락구조)

  • Jung, Hye-Ran;Jeon, Kwon-Seok;Kim, Jong-Kab;Moon, Hyun-Shik
    • Korean Journal of Environmental Agriculture
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    • v.27 no.4
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    • pp.421-427
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    • 2008
  • This study was carried out to offer a basis data for application of effective management through species composition and community structure of Castanopsis cuspidata var. thunbergii stands (Bijindo and Yokjido). The upper, middle and lower layer in Bijindo and Yokjido consisted of 10, 11 and 27 species, and 9, 6 and 28 species, respectively. The importance value of Castanopsis cuspidata var. thunbergii were highest in upper and middle layer both two studied site. The herbaceous species were 33 and 47 species in Bijindo and Yokjido, respectively. The species diversity ranged from 0.523 to 1.280 in Bijindo and 0.699 to 1.364 in Yokjido. Dominance in Bijindo and Yokjido were 0.477 and 0.242 at upper layer, 0.231 and 0.102 at middle layer, and 0.106 and 0.057 at lower layer, which showed that Castanopsis cuspidata var. thunbergii stands are dominated by a large number of species at all layer. The distribution pattern by Morisita's index showed that Castanopsis cuspidata var. thunbergii was distributed randomly in upper layer both the studied sites.

Analysis microstructure and mechanical properties of AlCr-based cutting tool coatings (AlCr계 절삭공구 코팅의 미세조직 및 우수한 기계적 물성 분석)

  • Im, Gi-Seong;Kim, Yeong-Seok;Park, Hye-Jin;Mun, Sang-Cheol;Jeong, Se-Il;Kim, Gwang-Sik;Park, Yeong-Gun;Kim, Gi-Beom
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2018.06a
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    • pp.131-131
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    • 2018
  • 최근 절삭공구산업은 자동차, 항공기, IT, 선박, 에너지 등 첨단산업의 증가로 인해 CGI, CFRP, 내열합금 등 난삭재의 수요가 증가하고 있다. 난삭재는 고내열, 고경도, 초경량 같은 특성을 지니며 우수한 기계적 물성을 갖지만 가공의 어려움이 있어 산업에 적용하는데 한계가 있다. 이러한 한계를 극복하기 위해 개발된 가공기술 중 하드 코팅은 공구코팅비용 대비 공구의 표면경도와 수명을 효율적으로 향상시킬 수 있다고 알려져 있다. 대표적인 하드코팅으로는 AlN계, TiN계 코팅이 있다. 이러한 코팅의 경우 높은 기계적 물성과 우수한 내마모성으로 인해 절삭공구의 성능을 향상시킬 수 있기 때문에, 많은 연구가 진행되고 있으며 절삭공구산업에서 각광받고 있다. 기존 선행연구 결과에 따르면 질화물 코팅의 우수한 물성은 질화물(Nitride) 생성 및 질화 공정에 의한 코팅층의 고밀도화에 의해 나타난다고 알려져 있다. 그 중에서 AlCrN coating은 우수한 내마모성 및 향상된 고온경도를 갖고 있다. AlCrN based coating에 미량의 원소를 첨가하여 기존 AlCrN coating의 기계적 특성을 더욱 향상 시킨 coating은 일반적인 고성능 코팅 대비 공구수명이 길다고 알려져 있으며, 전반적으로 우수한 특성에 의해 전 세계적으로 습식 및 건식 기계 가공 용도로 사용되고 있다. 본 연구에서는 AlCrN based coating에 미량의 원소를 첨가한 coating의 우수한 기계적 특성의 원인을 규명하기 위해 텅스텐카바이드(WC) 기판 위에 아크 이온 플레이팅 장비를 이용하여 AlCrN based coating을 증착 시킨 sample을 분석하였다. 결정구조 및 상 분석을 위해 X선 회절분석(XRD)을 실시하였으며, 미세 구조를 분석하기 위해 전계방출형 주사전자현미경(FE-SEM), 투과 전자현미경(TEM) 분석을 실시하였다. 또한 코팅층의 화학적 성분 분석을 위해 EDX분석을 실시하였으며 기계적 특성 평가를 위해 나노압입시험(Nano-indentation test)을 진행하였다.

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A Study on the Size System for Women's Suits and the Customer Satisfaction with Fitness in China -With the Focus on the Adult Female Residents of Beijing- (중국 기성복의 호칭과 맞음새 관련 소비자 만족도 조사 -북경에 거주하는 성인 여성을 중심으로-)

  • Seok Hye-Jung;Kim In-Suk
    • Journal of the Korean Society of Clothing and Textiles
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    • v.30 no.1 s.149
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    • pp.125-136
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    • 2006
  • The purpose of this study was to examine the size system of women's suits on the market in China and the levels of satisfaction on the fit experienced by Chinese customers, and thereby to look into the problems relating to the fit of Chinese women's suits. The findings were as follows: 1. The size fur women's suits currently on sale in China was indicated by the number by all manufacturers. The size of the largest production was in the order of 165-88A, 160-84A, and 170-92A for jackets; 160-68A, 165-72A, and 170-76A far pants and skirt. 2. The survey on the recognition of the size for ready-made suits showed the response rate of $91.1\%$ for jackets, $58.7\%$ for pants, and $56.3\%$ for skirts, indicating the higher recognition level in the measure for jackets in comparison with pants. 3. The survey on the satisfaction with the fitness for each item of women's suits(jackets, pants, and skirts) showed the satisfaction levels of $3.05\~3.63$ for all items, above the ordinary figures. Of the three items, the satisfaction level of pants was lowest. 4. The survey on the repair frequency for each item of suits showed $53.2\%$ for jackets, $52.5\%$ for pants, and $30.4\%$ for skirts, that of the skirts being lower than those of the other items.

Isolation and Characterization of a Ds-tagged liguleless Mutant in Rice (Oryza sativa. L)

  • Ahn, Byung-Ohg;Ji, Sang-Hye;Yun, Doh-Won;Ji, Hyeon-So;Park, Yong-Hwan;Park, Sung-Han;Lee, Gi-Hwan;Suh, Seok-Cheol;Lee, Myung-Chul
    • Journal of Crop Science and Biotechnology
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    • v.11 no.4
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    • pp.237-242
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    • 2008
  • A liguleless mutant, which showed complete loss of lamina joint region at the junction between leaf blade and leaf sheath, was isolated from a Ds insertional mutants derived from the source cultivar, Dongjin. This mutant could not affect other developmental patterns like phyllotaxis. Southern blot analysis, using GUS as a probe, revealed that the liguleless mutant contained three Ds copies transposed in the rice genome. Among the four genomic sequences flanking the Ds, one was mapped in the intergenic region (31661640 - 31661759), and the other two predicted a protein kinase domain (12098980 - 12098667) as an original insertion site within a starter line used for massive production of Ds insertional mutant lines. Another predicted and inserted in first exon of liguleless 1 protein (OsLG1) that was mapped in coding region (LOC_Os04g56170) of chromosome 4. RT-PCR revealed that the OsLG1 gene was not expressed liguleless mutants. Structure analysis of OsLG1 protein revealed that it predicted transcription factor with a highly conserved SBP domain consisting of 79 amino acids that overlapped a nuclear localization signal (NLS). RT-PCR revealed that OsLG1 is mainly expressed in vegetative organs.

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Color Deepening and Antimicrobial Finish in the Dyeing of Cotton Fabrics using Rhus verniciflua Extract (옻 추출물을 이용한 면직물 염색에서의 농색화 및 항미생물성 가공)

  • Jang, Yong-Joon;Choi, Young-Hwan;Lee, Hye-Mi;Tak, Mi-So;Lyoo, Won-Seok;Jang, Jin-Ho
    • Textile Coloration and Finishing
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    • v.23 no.1
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    • pp.28-34
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    • 2011
  • Deep coloration of cotton fabrics with concentrated Rhus verniciflua extract was carried out using large amount of Glauber's salt and a mordant in order to improve dyeability and functional properties such as deodorizing and antimicrobial activity. With increasing in the salt addition upto 80% in the dyeing liquor containing 0.45% extract concentration, K/S value and exhaustion increased threefold from 1.2 to 3.5, indicating that the salt reduced the electrostatic repulsion between the dyes and the cotton fibers. Also the concentrated extract solution to 8% can increase the color build up upto a K/S of 11.1. In addition the combined pre- and post-mordanting methods with potassium alum enhanced the dyeability upto a K/S of 22.2. The ammonia deodorizing property increased with increased color yield of the dyed fabrics. Also the dyed and post-mordanted fabrics with 8% extract concentration showed antimicrobial activity against both Klebsiella pneumoniae and Staphylococcus aureus.