• Title/Summary/Keyword: Hye-seok

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Characterization and RT-PCR Detection of dsRNA Mycoviruses from the Oyster Mushroom, Pleurotus ostreatus

  • Seo, Jang-Kyun;Lim, Won-Seok;Jeong, Ji-Hye;Yoo, Young-Bok;Yie, Se-Won;Kim, Kook-Hyung
    • The Plant Pathology Journal
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    • v.20 no.3
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    • pp.200-205
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    • 2004
  • The partial nucleotide sequences of the genomic dsRNA mycovirus infecting Pleurotus ostreatus isolates ASI2223 and Suhan were determined and compared with those of mycoviruses belonging to partitiviruses and totiviruses. Partial nucleotide sequences of the purified dsRNA from ASI2223 and Suhan showed RNA-dependent RNA polymerase sequences that are closely related to those of partitiviruses, including Fusarium poae virus 1, Fusarium solani virus, Rhizoctoniasolani virus, Discula destructiva virus 2, and Oyster mushroom isometric virus 2. Specific primers were designed for RT-PCR detection of dsRNA viruses from the P. ostreatus isolate ASI2223 and Suhan. Two virus specific primer sets were found to specifically detect each virus among six sets of designed oligonucleotide primers. Collectively, these results suggest that dsRNA mycoviruses from P. ostreatus isolates ASI2223 and Suhan belong to the family Partitiviridae, although, they are not the same virus species. Our results also suggest that these virus-specific primer sets can be employed for the specific detection of each viral sequence in infected tissues.

The Influence of the Pattern of Gripping the Ultrasound Head on the Activity of Upper Limb Muscles (초음파 도자를 쥐는 방법이 팔 근육의 근활성도에 미치는 영향)

  • Choi, Seok-Ho;Yi, Chung-Hwi;Jeon, Hye-Sun;Lee, Jeong-Weon
    • Physical Therapy Korea
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    • v.20 no.2
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    • pp.28-37
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    • 2013
  • This study examined differences in the activity of upper limb muscles according to how an ultrasound head is gripped. Twenty-two adult males were participated in the study. Each participant was asked to apply ultrasound treatment on to a lump of pork meat by two different ultrasound head grip patterns: spherical and cylindrical grips. Muscle activity was measured in the extensor carpi radialis longus (ECRL), flexor carpi ulnaris (FCU), and pronator teres (PT), triceps brachii (TB), middle deltoid (MD), and upper trapezius (UT) muscles. There were no significant differences in the EMG signals of any muscle according to the ultrasound head grip pattern (p>.05). There were significant differences in the EMG signal of each type of muscle (p<.05). The EMG signal of UT was the lowest and that of TB was lower than ECRL and FCU. There were interactions between ECRL and FCU, between ECRL and PT, between FCU and ECRL, and between FCU and MD. The EMG signal of ECRL using the cylindrical head was low and that of FCU with the cylindrical head was high, while the opposite was the case with the spherical head ($p_{adj}$ <.05/15). The results of this study indicate that the wrist muscles worked actively when the participants applied ultrasound therapy using both spherical and cylindrical heads. A spherical head might induce imbalanced muscle activity among the wrist muscles, leading to deviation of the wrist joint. Therefore, the cylindrical head is recommended for ultrasound therapy because it produced a constant, repeated force.

An Analytical Study on Feasibility of NCS Level of Qualification Redesign Categories in 7 Major Areas (7대 분야 자격재설계 종목의 NCS 수준의 타당성 분석연구)

  • Kang, Seok Joo;Woo, Mi Hye;Park, Young Sam
    • Journal of Engineering Education Research
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    • v.19 no.1
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    • pp.47-53
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    • 2016
  • New occupational qualification system built on NCS(National Competency Standard) aimed to induce industries, the final appliers of qualification, to play a leading role in designing qualification and to spontaneously apply such qualification standard to employment and promotion so as to reinforce universality of qualification. In this study, we verified the validity of NCS level of qualification categories for 130 occupational fields by redesigning NCS-based new occupational qualification system with a focus on 7 major areas, including mechanical field, which were found to have wide-ranging social ripple effect throughout society and high acceptability in 2014. The results of study on suitability at qualification level suggested the followings: First, there is a difference in number of competency units for each qualification category, depending on area. New qualification covering 7 major areas included 15.3 competency units for each qualification category, which exceeds the number of competency units(about 10 units) in subdivided NCS. By field, some difference was found with 7.9 competency units for cultural field and 22.6 competency units for information and communication area. Second, preference above normal level was observed in the criteria, procedure and method for determining qualification redesigning. However, preference below normal level was exhibited in connection with possibility of linkage with overseas qualifications, although preference was above normal level in relation to the suitability of design/qualification level and qualification categories based on lifelong career development path within scope of NCS as the criteria for determining the redesign of qualification categories. Second, we compared NCS level and SC(Sector Council level, and the results showed that NCS level was found to be somewhat well-defined. For the qualifications with different definitions, it was deemed difficult to determine superiority between NCS level and SC level. However, majority opinion indicated that it would be desirable to follow NCS level because NCS was created through collection of opinions for a prolonged period.

Identification of a novel mutation in a patient with pseudohypoparathyroidism type Ia

  • Lee, Ye Seung;Kim, Hui Kwon;Kim, Hye Rim;Lee, Jong Yoon;Choi, Joong Wan;Bae, Eun Ju;Oh, Phil Soo;Park, Won Il;Ki, Chang Seok;Lee, Hong Jin
    • Clinical and Experimental Pediatrics
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    • v.57 no.5
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    • pp.240-244
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    • 2014
  • Pseudohypoparathyroidism type Ia (PHP Ia) is a disorder characterized by multiform hormonal resistance including parathyroid hormone (PTH) resistance and Albright hereditary osteodystrophy (AHO). It is caused by heterozygous inactivating mutations within the Gs alpha-encoding GNAS exons. A 9-year-old boy presented with clinical and laboratory abnormalities including hypocalcemia, hyperphosphatemia, PTH resistance, multihormone resistance and AHO (round face, short stature, obesity, brachydactyly and osteoma cutis) which were typical of PHP Ia. He had a history of repeated convulsive episodes that started from the age of 2 months. A cranial computed tomography scan showed bilateral calcifications in the basal ganglia and his intelligence quotient testing indicated mild mental retardation. Family history revealed that the patient's maternal relatives, including his grandmother and 2 of his mother's siblings, had features suggestive of AHO. Sequencing of the GNAS gene of the patient identified a heterozygous nonsense mutation within exon 11 (c.637 C>T). The C>T transversion results in an amino acid substitution from Gln to stop codon at codon 213 ($p.Gln213^*$). To our knowledge, this is a novel mutation in GNAS.

Benzoic Acid Production with Respect to Starter Culture and Incubation Temperature during Yogurt Fermentation using Response Surface Methodology

  • Yu, Hyung-Seok;Lee, Na-Kyoung;Jeon, Hye-Lin;Eom, Su Jin;Yoo, Mi-Young;Lim, Sang-Dong;Paik, Hyun-Dong
    • Food Science of Animal Resources
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    • v.36 no.3
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    • pp.427-434
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    • 2016
  • Benzoic acid is occasionally used as a raw material supplement in food products and is sometimes generated during the fermentation process. In this study, the production of naturally occurring yogurt preservatives was investigated for various starter cultures and incubation temperatures, and considered food regulations. Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus paracasei, Lactobacillus reuteri, Lactobacillus plantarum, Bifidobacterium longum, Bifidobacterium lactis, Bifidobacterium bifidum, Bifidobacterium infantis, and Bifidobacterium breve were used as yogurt starter cultures in commercial starters. Among these strains, L. rhamnosus and L. paracasei showed the highest production of benzoic acid. Therefore, the use of L. rhamnosus, L. paracasei, S. thermophilus, and different incubation temperatures were examined to optimize benzoic acid production. Response surface methodology (RSM) based on a central composite design was performed for various incubation temperatures (35-44℃) and starter culture inoculum ratios (0-0.04%) in a commercial range of dairy fermentation processes. The optimum conditions were 0.04% L. rhamnosus, 0.01% L. paracasei, 0.02% S. thermophilus, and 38.12℃, and the predicted and estimated concentrations of benzoic acid were 13.31 and 13.94 mg/kg, respectively. These conditions maximized naturally occurring benzoic acid production during the yogurt fermentation process, and the observed production levels satisfied regulatory guidelines for benzoic acid in dairy products.

Volatile Components of Traditional Gochujang Produced from Small Farms according to Each Cultivation Region (지역별 소규모 농가 생산 전통 고추장의 휘발성 성분에 관한 연구)

  • Hong, Yeo Joo;Son, Seong Hye;Kim, Ha Youn;Hwang, In Guk;Yoo, Seung Seok
    • Journal of the East Asian Society of Dietary Life
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    • v.23 no.4
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    • pp.451-460
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    • 2013
  • The purpose of this study is to investigate the volatile compounds of Korean traditional gochujang from various districts. The volatiles from each traditional gochujang are being extracted by simultaneous steam distillation extraction (SDE), and analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). Twenty compounds are identified as major volatile components which include 8 esters, 4 alcohols and 4 acids. The most traditional gochujang possesses more volatile components rather than commercial gochujang products. Most acids come from fatty acids and the alcohols derive from the oxidative degradation of linolenic acid. The most abundant volatile compounds for both traditional and commercial gochujang include 10 compounds such as 2-methyl-1-propanol, hexanal, 2-methyl-1-butanol, octanoic acid ethyl ester, as well as the various type of acids and esters. They represent most of the total GC peak areas, respectively. From the results, the characteristics of the flavors for traditional gochujang from each district are not clear but have shown various components than the commercial products.

Molecular Identification of Haemadipsa rjukjuana (Hirudiniformes: Haemadipsidae) in Gageo Island, Korea

  • Won, Sohyun;Park, Bae Keun;Kim, Baek Jun;Kim, Hye Won;Kang, Jun Gu;Park, Tae Seo;Seo, Hong Yul;Eun, Ye;Kim, Ki Gyoung;Chae, Joon Seok
    • Parasites, Hosts and Diseases
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    • v.52 no.2
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    • pp.169-175
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    • 2014
  • There are 60 species of blood-feeding land leeches, 50 species belonging to the family Haemadipsidae and 10 species belonging to the family Xerobdellidae. Despite recent papers on the land leeches, their taxonomic identification is not fully understood, especially at a species level. In Korea, there have been no historical records of the terrestrial leeches, but recently an unrecorded blood-feeding land leech was discovered at Gageo-do (Island), Korea. Molecular analysis was used to identify the species of 29 leeches collected from Mt. Dock-Sil in Gageo-do. Conventional PCR was conducted using nuclear 18S rRNA and mitochondrial cytochrome c oxidase subunit 1 (CO1) genetic marker. The 18S rRNA sequences revealed that the leeches share 99.9% identity with Haemadipsa rjukjuana (inhabiting Taiwan), and the CO1 sequences revealed that the leeches are very close to H. rjukjuana (inhabiting Taiwan). The CO1 sequences were separated into 2 categories, 1 with 94.6% and the other with 94.3% similarity to the H. rjukjuana L00115A (inhabiting Taiwan). This new finding of the land leech is the first record in Korea. In addition, the north range of the distribution of the blood-feeding leech (Hirudiniformes: Haemadipisidae) should be reconsidered including Korea.

Designing Mobile User Interface with Grip-Pattern Recognition (파지 형태 인식을 통한 휴대 단말용 사용자 인터페이스 설계)

  • Chang, Wook;Kim, Kee-Eung;Lee, Hyun-Jeong;Cho, Joon-Kee;Soh, Byung-Seok;Shim, Jung-Hyun;Yang, Gyung-Hye;Cho, Sung-Jung;Park, Joon-Ah
    • 한국HCI학회:학술대회논문집
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    • 2006.02a
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    • pp.678-683
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    • 2006
  • A novel and intuitive way of accessing applications of mobile devices is presented. The key idea is to use grip-pattern, which is naturally produced when a user tries to use the mobile device, as a clue to determine an application to be launched. To this end, a capacitive touch sensor system is carefully designed and installed underneath the housing of the mobile terminal to capture the image of the user's grip-pattern. The captured data is then recognized by a recognizer with dedicated preprocessing and postprocessing algorithms. The recognition test is performed to validate the feasibility of the proposed user interface system.

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Development of Retirement Prediction Model based on Work Life Profile Using Machine Learning Method (기계 학습 방법을 이용한 직장 생활 프로파일 기반의 퇴직 예측 모델 개발)

  • Yun, You-Dong;Lee, Seol-Hwa;Ji, Hye-Sung;Lim, Heui-Seok
    • The Journal of Korean Association of Computer Education
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    • v.20 no.1
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    • pp.87-97
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    • 2017
  • Recently, much research has been done on the turnover and retirement intentions of the organization members as many companies recognize the negative impact of the human resource outflow on the organization. However, most of the studies are conducted in the form of questionnaires, and there is still a lack of studies on the turnover and retirement intentions based on the work life data. In this study, we analyzed the factors affecting the retirement of employees based on the work life profile, and created a retirement prediction model using the machine learning method. As a result, we could identify various factors that were not covered in previous researches. In addition, we have established a basis for research that can provide a solution for the problem of human resource outflow by generating a good performance retirement prediction model.

Development of Smart Senior Classification Model based on Activity Profile Using Machine Learning Method (기계 학습 방법을 이용한 활동 프로파일 기반의 스마트 시니어 분류 모델 개발)

  • Yun, You-Dong;Yang, Yeong-Wook;Ji, Hye-Sung;Lim, Heui-Seok
    • Journal of the Korea Convergence Society
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    • v.8 no.1
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    • pp.25-34
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    • 2017
  • With the recent spread of smartphones and the introduction of web services, online users can access large-scale content regardless of time or place. However, users have had trouble finding the content they wanted among large-scale content. To solve this problem, user modeling and content recommendation system have been actively studied in various fields. However, in spite of active changes in senior groups according to the changes in information environment, research on user modeling and content recommendation system focused on senior groups are insufficient. In this paper, we propose a method of modeling smart senior based on their preference, and further develop a smart senior classification model using machine learning methods. As a result, we can not only grasp the preferences of smart seniors, but also develop a smart senior classification model, which is the foundation for the research of a recommendation system which will provide the activities and contents most suitable for senior groups.