• Korean Journal of Plant Resources
• /
• v.25 no.4
• /
• pp.490-497
• /
• 2012

Phylogenetic analyses were conducted to evaluate relationships of 5 taxa of Korean Hydrocotyle, H. spp. found in the Ulleung island including one outgroup (Centella asiatica). The molecular phylogenetic methods based on nuclear ribosomal DNA ITS region and cpDNA trnH-psbA region. Centella asiatica was used outgroup for analysis. As the result, genus Korean Hydrocotyle were grouped by 94% bootstrap value. Korean Hydrocotyle was grouped by four clades; Clade I-H. maritima, H. sibthorpides and H. yabei clade Clade II-H. nepalensis clade clade III-H. ramiflora clade clade IV-H. spp. clade. H. maritima, H. sibthorpides and H. yabei was not distinguished, seperately. H. spp. was distinctly distinguished other Korean Hydrocotyle.

• Biomedical Science Letters
• /
• v.27 no.4
• /
• pp.277-282
• /
• 2021

Hydrocotyle is a genus of prostrate, perennial aquatic or semi-aquatic plants formerly classified in the family Apiaceae, now in the family Araliaceae. Lipoxygenases (LOX) are present in the human body and play an important role in the stimulation of inflammatory reactions. Ethanolic extracts of five Hydrocotyle species (H. ramiflora, H. maritima, H. nepalensis, H. sibthorpioides, and H. yabei) showed inhibition of 23.5~50.6% at 2.0 mg/mL. Their extracts showed LOX inhibition in half maximal effective concentration (EC₅₀) range 15.1~15.7 ㎍/mL. Hyaluronic acid is a glycosaminoglycan, a major component of the extracellular matrix Five extracts of these species inhibited less than 23.0% of Hyaluronidase (HAase) activity at a concentration of 2.0 mg/mL Xanthine oxidase (XO) is a form of xanthine oxidoreductase, a type of enzyme that generates reactive oxygen species. Five Hydrocotyle species were found to have inhibitory activity of XO at 2.0 mg/ml, with 65% having greater than 50% inhibition. H. ramiflora exhibited the highest activity with an inhibition of 80.0%. The results suggested that Lipoxygenases, Hyaluronidase, and Busan 47340, Republic of Korea from five Hydrocotyle species might be multifunctional and prevent the degradation of allergic reactions and inflammation.

• Korean Journal of Medicinal Crop Science
• /
• v.11 no.5
• /
• pp.336-339
• /
• 2003

Hydrocotyle maritima Honda used as medicinal plants for a hemostatic agent was investigated for in vitro regeneration. The petiole explants of H. maritima were cultured on callus induction medium containing growth regulators ($0{\sim}5\;mg/l$, NAA and $0{\sim}5\;mg/l$ 2,4-D) either in single or in combination with $0.1{\sim}2\;mg/l$ BA for 6 weeks. Although single treatments of 2,4-D or NAA resulted in callus formation, the best results were combination of $0.5\;mg/l$, 2,4-D and $0.5\;mg/l$, BA. $5\;mg/l$, NAA and $0.5\;mg/l$, BA, respectively. The highest number of shoot (12 shoots per callus) was achieved with $3\;mg/l$, Kinetin. Also, when pieces of embryogenic callus induced on the medium supplemented with $0.5\;mg/l$, 2,4-D and $0.5\;mg/l$, BA were subcultured on hormone-free medium, somatic embryos were differentiated and developed further into welldeveloped plants.

• 한국생물공학회:학술대회논문집
• /
• 2002.04a
• /
• pp.331-332
• /
• 2002

• Journal of Plant Biology
• /
• v.39 no.1
• /
• pp.15-22
• /
• 1996

Seventy-three chromosome counts are reported from populations in 70 species from Korea, of which 34 counts in 32 species belong to Apiaceae. New species counts are in Acanthopanax chiisanensis(2n=48), Hydrocotyle japonica(2n=ca.96), H. maritima (n=12), Libanotis coreana (2n=22), Lindera sericea (n=12), Quercus myrsinaefolia (n=12), Rhamnus yoshinoi (n=12), Salix hulteni (n=19), Symplocos chinensis for. pilosa (n=11) and Vaccinium oldhami (n=12). New chromosome level for Asperula odorata (n=11), Cryptotaenia japonica (2n=16) and Sium ninsi (2n=18) is also provided. The taxonomic implications of certain of these counts are discussed.

• Journal of Ecology and Environment
• /
• v.34 no.3
• /
• pp.295-305
• /
• 2011

Many studies have suggested the positive effects of grazing by large herbivorous mammals on seed dispersal, but little is known about how herbivores could affect the fate of ingested seeds. This study examined the effects of seed ingestion by sika deer (Cervus nippon) on seed fate in a temperate grassland established in an urban park long resided by high densities of sika deer. I compared species composition and seasonal traits of seed abundance and maturity in the grassland community with those in deer fecal pellets. In total, 27 herbaceous species were observed, including the predominant Zoysia japonica. Seed phenology and production differed among the three dominant species (Z. japonica, Digitaria violascens, and Hydrocotyle maritima). Pellets contained at least 26 species of herbaceous seeds, and their abundance differed among species. Of the 26 species, 15 were observed in the vegetation at the study site. The peak of seed abundance in pellets for the dominant species appeared 1 month after the peak of inflorescence production (but most of the inflorescences were immature and susceptible to digestion) and consequently corresponded to the peak of mature inflorescence. Because sika deer are likely to ingest seeds at any maturity stage in the grassland and immature seeds are less hardened, ingested immature seeds can suffer great losses. The results suggested that the survival of germable seeds with great losses of immature seeds may be a factor determining which plant species can be successfully dispersed by herbivores.

• Journal of the Speleological Society of Korea
• /
• v.56
• /
• pp.15-38
• /
• 1998

Biomass and soil properties of plant communites was investigated in the limestone ares, Tanyang, Ch'ungbuk Province in Korea. Plants was classified in calcicoles and calcifuges within plant communities occurring in the limestone area. Biomass of plant populatins in the limestone area was measured that Stellaria aquatica was the lowest(0.58g/plant), Chrysanthemum boreale was the highest(8.87g/plant) and that Pennisetum alopecuroides, Miscanthus sinensis was 6.67g/plant, 5.76g/plant, respectively. Diameter of breast height among the tree layer communities was investigated that Pinus densiflora was the widest(20cm), Juniperus rigida was the narrowest(6cm) and that Quercus aliena, Populus ${\times}$ tomentiglandulosa was 10cm, 12cm, respectively. The high of density in the tree layer was found out Populus ${\times}$ tomentiglandulosa, Juniperus rigida, Quercus aliena, Pinus densiflora in this order. Dominant species in the study area were Pinus densiflora, Quercus aliena, Juniperus rigida, Quercus dentata in the tree layer, Lespedeza bicolor, Buxus microphylla, Rhus chinensis in the shrub layer, Humulus Japonicus, Erigeron canadensis, Xanthium strumarium, Oenothera odorata Persicaria hypropiper on the riverside, Pennisetum alopecuroides, Vicia amoena, Chrysanthemum boreale on the roadside. Soil properties of the limestone area was investigated around the protrusion of rock in the upper region, around the cave and underlayer accumulated by corrosion in the study area. Lime-chlorosis was observed in the upper region, underlayer and around the cave. It was observed that biota of kum-gul and dungbong-gul was Rhinolophus ferrumequinum korai, Epanerchodus kimi, Antrokoreana gracilipes, Diestrammena japonical. Dominant species were Humulus japonicus, Stellaria aquatica, Hydrocotyle maritima, Phragmites communis, Miscanthus sinensis around the inlet of a kum-gul. It was classified that plants in the study area was 34 order, 53 family, 135 species in all. Calcicoles were 11 order, 16 family, 18 species and calcufuges were 6 order, 8 family, 11 species of them all.