• Journal of Convergence for Information Technology
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• v.9 no.6
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• pp.233-241
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• 2019

This study was conducted on women in their 20s and 30s living in the Seoul metropolitan area by using questionnaires on the recognition and purchase behavior of mask packs. According to this study, although there is high interest in skin beauty, the recognition of characteristics and distinctions according to mask pack type was found to be insufficient. After using mask packs, 51.5% of those surveyed were satisfied with their efficacy and effectiveness. When using the mask pack, the Sheet type mask pack was discontented with usability, size, Close Adhesion and skin irritation, Hydrogel type is material, sleeping type is content and absorbent, cellulose type pack was found to have the same discomfort with the material as the hydro gel type. Through the results of this study, the possibility of utilization as basic data for mask pack market marketing was confirmed.

• Genomics & Informatics
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• v.2 no.4
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• pp.174-179
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• 2004

We developed various plasmid cloning vectors that are useful in the construction of genomic and shotgun libraries. Two medium copy vectors, pCUGlblu21 (pCb21) and pAGlblu21 (pAb21), which are resistant to kanamycin ($Km^R$) and chloramphenicol ($Cam^R$), respectively, are useful for cloning DNA inserts ranging from 5kb to 15kb. Two high copy vectors, pCUGlblu31 (pCb31) and pAGlblu31 (pAb31), containing $Km^R$ and $Cam^R$, respectively, are useful for DNA inserts less than 5kb. These vectors are well adapted for large-scale genome sequencing projects by providing choice of copy number and selectable marker. The small vector size is another advantage of these vectors. All vectors contain lacZa including multicloning sites that originated from pBluscriptllsk- for easy cloning and sequencing. Two medium copy vectors contain unique and rare cutting Swal (ATTTAAAT) restriction enzyme sites for easy determination of insert size. We developed two combined vectors, pC21A31 and pC31A21, which are combinations of (pCb21 + pAb31) and (pCb31 + pAb21), respectively. These two vectors provide four choices of vectors such as $Km^R$ and medium, $Cam^R$ and high, $Cam^R$ and medium, and $Km^R$ and high copy vectors by restriction enzyme cutting, dephosphorylation, and gel purification. These vectors were successfully applied to high throughput shotgun sequencing of rice, tomato, and brassica BAC clones. With an example of extremely biased hydro sheared 3 kb shotgun library of a tomato BAC clone, which is originated from cytogenetically defined peri-centromeric region, we suggest the utility of an additional 10 kb library for sequence assembly of the difficult-to-assemble BAC clone.