• 제목/요약/키워드: Hybrid nuclear system

검색결과 97건 처리시간 0.024초

Yeast의 FLP/FRT 시스템을 이용한 BmNPV의 유전자 재조합 (Construction of Recombinant Bombyx mori Nuclear Polyhedrosis Virus Using a FLP/FRT System of Yeast, Saccharomyces cerevisiae 2$\mu$m plasmid)

  • 강석우;윤은영
    • 한국잠사곤충학회지
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    • 제40권1호
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    • pp.52-59
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    • 1998
  • For the construction of plasmid and bmNPV sarrying the FRT recognition site for the FLP recombinases, we synthesized the wild type FRT dligonucleotides. The target FRT sequences consist of three 13bp repeated DNA sequences; two repeats in a direct orientation and one inverted relative to the other two. In addition, there is an 8bp spacer region between the repeats which determune the orientation of the FRT recombination site. In order to place the FRT site both in target BmNPV genome and the transfer vector, we constructed a plasmid, FRT site both in the target BmNPv genome and the transfer vector, we constructed a plasmid, pFRT$\beta$-gal, carrying the FRT sites within the cloning sites of pSV vector and a recombinant BmNPV, vFRTPH, carrying the FRT sites at a downstream of polyhedrin promotor, respectively. In order to test the functionality of the FLP/FRT site-specific recombination system, vFRTPH, pFRT$\beta$-gal and pHsFLP DNA were co-transfected into BmN-4 cells. The resulting recombinant virus was designated a vFRT$\beta$2-gal. From construction analysis of the vFRT$\beta$2-gal with PCR technique it was concluded that the entire pFRT$\beta$-gal plasmid with $\beta$-galactosidase gene and origines of replication flanked by two functional hybrid FRT sequences. The efficiency of recombination was 8.7%, which was higher than that(2.2%) of recombination between a conventional transfer vector and the wild type BmNPV.

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Heavy concrete shielding properties for carbon therapy

  • Jin-Long Wang;Jiade J Lu;Da-Jun Ding;Wen-Hua Jiang;Ya-Dong Li;Rui Qiu;Hui Zhang;Xiao-Zhong Wang;Huo-Sheng Ruan;Yan-Bing Teng;Xiao-Guang Wu;Yun Zheng;Zi-Hao Zhao;Kai-Zhong Liao;Huan-Cheng Mai;Xiao-Dong Wang;Ke Peng;Wei Wang;Zhan Tang;Zhao-Yan Yu;Zhen Wu;Hong-Hu Song;Shuo-Yang Wei;Sen-Lin Mao;Jun Xu;Jing Tao;Min-Qiang Zhang;Xi-Qiang Xue;Ming Wang
    • Nuclear Engineering and Technology
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    • 제55권6호
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    • pp.2335-2347
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    • 2023
  • As medical facilities are usually built at urban areas, special concrete aggregates and evaluation methods are needed to optimize the design of concrete walls by balancing density, thickness, material composition, cost, and other factors. Carbon treatment rooms require a high radiation shielding requirement, as the neutron yield from carbon therapy is much higher than the neutron yield of protons. In this case study, the maximum carbon energy is 430 MeV/u and the maximum current is 0.27 nA from a hybrid particle therapy system. Hospital or facility construction should consider this requirement to design a special heavy concrete. In this work, magnetite is adopted as the major aggregate. Density is determined mainly by the major aggregate content of magnetite, and a heavy concrete test block was constructed for structural tests. The compressive strength is 35.7 MPa. The density ranges from 3.65 g/cm3 to 4.14 g/cm3, and the iron mass content ranges from 53.78% to 60.38% from the 12 cored sample measurements. It was found that there is a linear relationship between density and iron content, and mixing impurities should be the major reason leading to the nonuniform element and density distribution. The effect of this nonuniformity on radiation shielding properties for a carbon treatment room is investigated by three groups of Monte Carlo simulations. Higher density dominates to reduce shielding thickness. However, a higher content of high-Z elements will weaken the shielding strength, especially at a lower dose rate threshold and vice versa. The weakened side effect of a high iron content on the shielding property is obvious at 2.5 µSv=h. Therefore, we should not blindly pursue high Z content in engineering. If the thickness is constrained to 2 m, then the density can be reduced to 3.3 g/cm3, which will save cost by reducing the magnetite composition with 50.44% iron content. If a higher density of 3.9 g/cm3 with 57.65% iron content is selected for construction, then the thickness of the wall can be reduced to 174.2 cm, which will save space for equipment installation.

태양열과 지열을 이용한 난방용 공기순환시스템 기초연구 - 태양열을 이용한 트롬월식의 축열성능 중심으로 - (A Basic Study on the Air Circulation System for Heating using Solar and Geothermal Heat - Focused on Trombe Wall Thermal Storage Performance using Solar Heat -)

  • 김병윤;최용석
    • 한국농촌건축학회논문집
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    • 제19권4호
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    • pp.49-56
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    • 2017
  • Each country in the world currently concentrates on shifting into clean energy, which can be alternative energy, for global environment protection and solution to the problem of fossil fuel depletion. The Korean government is predicted to develop renewable energy, such as solar power, ground power, and offshore wind power, and to increase their supply ratios by ending the use of coals and nuclear power plants. This study conducted experiments on thermal storage performance of Trombe wall thermal storage materials using solar power and simulations in order to offer baseline data for the development of a hybrid air circulation system for heating that can maximize efficiency by simultaneously using solar and geothermal power. The study results are as follows: (1) In all the specimens with 3m, 5m, and 7m in the length of thermal storage pipe, $5.7^{\circ}C$, $7.8^{\circ}C$, and $10.5^{\circ}C$ rose, respectively, as the thermal storage effect of the specimens attaching insulation film and black tape to the general funnel. They were most excellent in terms of thermal storage effect. (2) As a result of thermal performance evaluation on the II type specimens, II-3 ($7.8^{\circ}C$ rise) > II-4 ($5.3^{\circ}C$ rise) > II-1 ($3.9^{\circ}C$ rise) > II-2 ($2.3^{\circ}C$ rise) was revealed, and thus II-3 (insulation film + black tape) was most effective as shown in the I type. (3) This study analyzed air current and temperature distribution inside of the greenhouse by linking actually measured values and simulation interpretation results through the interpretation of CFD (computational fluid dynamics). As a result, the parts absorbing heat and discharging heat around the thermal storage pipe could be visibly classified, and temperature distribution inside of the greenhouse around the thermal storage pipe could be figured out.

OPTHiS Identifies the Molecular Basis of the Direct Interaction between CSL and SMRT Corepressor

  • Kim, Gwang Sik;Park, Hee-Sae;Lee, Young Chul
    • Molecules and Cells
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    • 제41권9호
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    • pp.842-852
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    • 2018
  • Notch signaling is an evolutionarily conserved pathway and involves in the regulation of various cellular and developmental processes. Ligand binding releases the intracellular domain of Notch receptor (NICD), which interacts with DNA-bound CSL [CBF1/Su(H)/Lag-1] to activate transcription of target genes. In the absence of NICD binding, CSL down-regulates target gene expression through the recruitment of various corepressor proteins including SMRT/NCoR (silencing mediator of retinoid and thyroid receptors/nuclear receptor corepressor), SHARP (SMRT/HDAC1-associated repressor protein), and KyoT2. Structural and functional studies revealed the molecular basis of these interactions, in which NICD coactivator and corepressor proteins competitively bind to ${\beta}-trefoil$ domain (BTD) of CSL using a conserved ${\varphi}W{\varphi}P$ motif (${\varphi}$ denotes any hydrophobic residues). To date, there are conflicting ideas regarding the molecular mechanism of SMRT-mediated repression of CSL as to whether CSL-SMRT interaction is direct or indirect (via the bridge factor SHARP). To solve this issue, we mapped the CSL-binding region of SMRT and employed a 'one- plus two-hybrid system' to obtain CSL interaction-defective mutants for this region. We identified the CSL-interaction module of SMRT (CIMS; amino acid 1816-1846) as the molecular determinant of its direct interaction with CSL. Notably, CIMS contains a canonical ${\varphi}W{\varphi}P$ sequence (APIWRP, amino acids 1832-1837) and directly interacts with CSL-BTD in a mode similar to other BTD-binding corepressors. Finally, we showed that CSL-interaction motif, rather than SHARP-interaction motif, of SMRT is involved in transcriptional repression of NICD in a cell-based assay. These results strongly suggest that SMRT participates in CSL-mediated repression via direct binding to CSL.

새로운 혼합알고리즘을 이용한 CPFS 내에서의 일어나는 동적 열전달의 수식화 및 해석 (Modeling and analysis of dynamic heat transfer in the cable penetration fire stop system by using a new hybrid algorithm)

  • 윤인섭;윤종필;권성필
    • 한국가스학회지
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    • 제7권4호
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    • pp.44-52
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    • 2003
  • 본 논문은 원자력발전소 방화벽에 설치된 케이블관통부 충전시스템(CPFS: Cable penetration fire stop) 안에서 일어나는 동적 열전달 현상을 수식화하고, 새로운 혼합알고리즘을 이용해서 수치적으로 계산하여, 3차원 그래픽으로 나타내는 작업에 관한 연구이다. CPFS 내에서의 열전도 현상을 주어진 초기조건과 경계조건하에서 포물선 편미분방정식(Parabolic PDE)으로 수식화하였다. 계산을 단순화하기 위하여 전체 열 흐름을 z-축직선상에서의 일어나는 열전도 성분과 x-y-좌표 평면상에서 일어나는 열전도 성분으로 나누었다. z-축과 평행한 직선상에서 일어나는 열전도를 나타내는 PDE는 연속과완화법(SOR: Sequential over-relaxation)을 이용하여 유한불연속 점들에 대한 연립상미분방정식(ODE)으로 만들어서 풀었고, x-y-좌표 평면상에서 일어나는 열전도에 관한 PDE는 Galerkin 유한요소법(FEM: Finite element method)을 적용하여 ODE로 전환해서 풀었다. 여기서 시간과 공간의 함수인 온도는 각 직선상의 점들과 각 평면상의 요소절점들에 대해서 일정한 시간간격으로 초기온도와 경계온도를 업데이트하여 번갈아 가며 계산한다. 이러한 일련의 계산결과를 바탕으로 CPFS시스템 내에서의 온도분포의 동적인 변화를 계산해 낼 수 있었다. 결론적으로 관통하는 케이블이 CPFS시스템의 온도분포에 매우 중요한 역할을 한다는 것을 알 수 있었다. 시뮬레이션 결과는 CPFS내의 온도분포를 쉽게 이해할 수 있도록 3차원 그래픽으로 나타냈으며, 관통하는 케이블이 방화시스템의 온도분포에 매우 중요한 영향을 끼친다는 것을 알 수 있었다. 마지막으로 계산결과를 실험결과와 직접 비교함으로써, 개발된 모델과 계산 알고리즘의 정당성을 보였다.

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설비공학 분야의 최근 연구 동향 : 2013년 학회지 논문에 대한 종합적 고찰 (Recent Progress in Air-Conditioning and Refrigeration Research : A Review of Papers Published in the Korean Journal of Air-Conditioning and Refrigeration Engineering in 2013)

  • 이대영;김사량;김현정;김동선;박준석;임병찬
    • 설비공학논문집
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    • 제26권12호
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    • pp.605-619
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    • 2014
  • This article reviews the papers published in the Korean Journal of Air-Conditioning and Refrigeration Engineering during 2013. It is intended to understand the status of current research in the areas of heating, cooling, ventilation, sanitation, and indoor environments of buildings and plant facilities. Conclusions are as follows. (1) The research works on the thermal and fluid engineering have been reviewed as groups of fluid machinery, pipes and relative parts including orifices, dampers and ducts, fuel cells and power plants, cooling and air-conditioning, heat and mass transfer, two phase flow, and the flow around buildings and structures. Research issues dealing with home appliances, flows around buildings, nuclear power plant, and manufacturing processes are newly added in thermal and fluid engineering research area. (2) Research works on heat transfer area have been reviewed in the categories of heat transfer characteristics, pool boiling and condensing heat transfer and industrial heat exchangers. Researches on heat transfer characteristics included the results for general analytical model for desiccant wheels, the effects of water absorption on the thermal conductivity of insulation materials, thermal properties of Octadecane/xGnP shape-stabilized phase change materials and $CO_2$ and $CO_2$-Hydrate mixture, effect of ground source heat pump system, the heat flux meter location for the performance test of a refrigerator vacuum insulation panel, a parallel flow evaporator for a heat pump dryer, the condensation risk assessment of vacuum multi-layer glass and triple glass, optimization of a forced convection type PCM refrigeration module, surface temperature sensor using fluorescent nanoporous thin film. In the area of pool boiling and condensing heat transfer, researches on ammonia inside horizontal smooth small tube, R1234yf on various enhanced surfaces, HFC32/HFC152a on a plain surface, spray cooling up to critical heat flux on a low-fin enhanced surface were actively carried out. In the area of industrial heat exchangers, researches on a fin tube type adsorber, the mass-transfer kinetics of a fin-tube-type adsorption bed, fin-and-tube heat exchangers having sine wave fins and oval tubes, louvered fin heat exchanger were performed. (3) In the field of refrigeration, studies are categorized into three groups namely refrigeration cycle, refrigerant and modeling and control. In the category of refrigeration cycle, studies were focused on the enhancement or optimization of experimental or commercial systems including a R410a VRF(Various Refrigerant Flow) heat pump, a R134a 2-stage screw heat pump and a R134a double-heat source automotive air-conditioner system. In the category of refrigerant, studies were carried out for the application of alternative refrigerants or refrigeration technologies including $CO_2$ water heaters, a R1234yf automotive air-conditioner, a R436b water cooler and a thermoelectric refrigerator. In the category of modeling and control, theoretical and experimental studies were carried out to predict the performance of various thermal and control systems including the long-term energy analysis of a geo-thermal heat pump system coupled to cast-in-place energy piles, the dynamic simulation of a water heater-coupled hybrid heat pump and the numerical simulation of an integral optimum regulating controller for a system heat pump. (4) In building mechanical system research fields, twenty one studies were conducted to achieve effective design of the mechanical systems, and also to maximize the energy efficiency of buildings. The topics of the studies included heating and cooling, HVAC system, ventilation, and renewable energies in the buildings. Proposed designs, performance tests using numerical methods and experiments provide useful information and key data which can improve the energy efficiency of the buildings. (5) The field of architectural environment is mostly focused on indoor environment and building energy. The main researches of indoor environment are related to infiltration, ventilation, leak flow and airtightness performance in residential building. The subjects of building energy are worked on energy saving, operation method and optimum operation of building energy systems. The remained studies are related to the special facility such as cleanroom, internet data center and biosafety laboratory. water supply and drain system, defining standard input variables of BIM (Building Information Modeling) for facility management system, estimating capability and providing operation guidelines of subway station as shelter for refuge and evaluation of pollutant emissions from furniture-like products.

원자력발전소 디지털형 주제어실 모형 개발 (Development of a Prototype for the Digitalized Nuclear Power Plant's Main Control Room)

  • 정연섭;조성제
    • 한국인터넷방송통신학회논문지
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    • 제9권4호
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    • pp.145-152
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    • 2009
  • 본 우리나라 고리원전 1호기의 주제어실은 2007년에 부분 개선되었고 2013년에 완전 개량형화 될 예정이다. 부분 개선을 통해 원래의 아나로그 설비를 일부 대체하는 디지털 제어설비가 도입되었으며 안전변수표시 장치같이 단독으로 사용되던 전산시스템이 발전소 전산시스템에 통합되었다. 2015년에는 디지털화를 바탕으로 한 한국표준원전주제어실의 개량이 예정되어 있으나 현장 엔지니어와 운전원들은 개량형주제어실에 대해 호의적이지 않은 실정이다. 따라서 현장 운전원과 엔지니어들의 수용성을 제고하고 또한 사용자 인터페이스와 계측제어 구조를 평가하기 위한 표준형원전의 개량형주제어실 모형이 개발되었으며 모형에는 운전원들의 업무 지원성을 향상시킬 수 있도록 화면이 다중 층으로 구성되며 층간에는 상황추종메뉴로 연결되어 있는 P&ID 베이스 디스플레이 시스템이 채택되었다. 이 시스템은 첫 번째 층에는 간략화 된 P&ID가 디스플레이 되고 계속해서 자세한 추가 정보를 디스플레이 할 수 있도록 고안하였다. 디지털 시스템의 신뢰성과 운전성을 고려한 한국표준원전 개량형주제어실(MCR)의 최종형태가 제시되었으며 추가해서 운전에 영향을 미치지 않도록 발전소 정기보수 기간 중에 주제어실 현대화 작업을 수행할 수 있는 방안이 제시되었다.

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