• Biomolecules & Therapeutics
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• v.30 no.6
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• pp.593-602
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• 2022

The human papillomavirus (HPV)-18 E7 (E7) oncoprotein is a major transforming protein that is thought to be involved in the development of cervical cancer. It is well-known that E7 stimulates tumour development by inactivating pRb. However, this alone cannot explain the various characteristics acquired by HPV infection. Therefore, we examined other molecules that could help explain the acquired cancer properties during E7-induced cancer development. Using the yeast two-hybrid (Y2H) method, we found that the Elk-1 factor, which is crucial for cell proliferation, invasion, cell survival, anti-apoptotic activity, and cancer development, binds to the E7. By determining which part of E7 binds to which domain of Elk-1 using the Y2H method, it was found that CR2 and CR3 of the E7 and parts 1-206, including the ETS-DNA domain of Elk-1, interact with each other. As a result of their interaction, the transcriptional activity of Elk-1 was increased, thereby increasing the expression of target genes EGR-1, c-fos, and E2F. Additionally, the colony forming assay revealed that overexpression of Elk-1 and E7 promotes C33A cell proliferation. We expect that the discovery of a novel E7 function as an Elk-1 activator could help explain whether the E7 has novel oncogenic activities in addition to p53 inactivation. We also expect that it will offer new methods for developing improved strategies for cervical cancer treatment.

• Composites Research
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• v.20 no.6
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• pp.1-7
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• 2007

Expanded graphite/carbon fiber hybrid conductive polymer composites were fabricated by the preform molding technique. The conductive fillers were mechanically mixed with a phenol resin to provide an electrical property to composites. The conductive filler loading was fixed at 60wt.% to accomplish a high electrical conductivity. Expanded graphites were excellent in forming a conductive networking by direct contacts between them while it was hard to get the high flexural strength over 40MPa with using only expanded graphite and phenol resin. In this study, carbon fibers were added in composites to compensate the weakened flexural strength. The effect of carbon fibers on the mechanical and electrical properties was examined according to the weight ratio of carbon fiber. As the carbon fiber ratio increased, the flexural strength increased until the carbon fiber ratio of 24wt.%, and then decreased afterward. The electrical conductivity gradually decreased as the increase of the carbon fiber ratio. This was attributed to the non-conducting regions generated among the carbon fibers and the reduction of the direct contact areas between expanded graphites.

• Journal of the Korean Institute of Rural Architecture
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• v.24 no.2
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• pp.21-28
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• 2022

Focusing on the creation of a new han ok, especially a mid-rise hybrid-structured Han-ok, this study proposes a middle-rise (four-story) Han-ok on one and two lots located in Seoun-dong, the existing Han-ok intensive housing site in downtown Cheongju. 1) In terms of layout and function, according to the existing L-shaped Han-ok corresponding to the road and the direction, the parking lots and shops on the 1st floor, the business facility on the 2nd floor, the Han-ok on the 3rd and 4th floors are placed. There are yards, open roof yards, and semi-open Daecheong(大廳, main hall), which can be shared by residents. 2) In terms of structure and form, one or two floors (some 3 floors) are 5.4m square and 5.4×6.6m modules of the RC(Reinforced Concrete) group, and the upper floor reflects the 2.7m module, size and shape of the existing Han-ok. By extending the outer wall of the RC group in the lower floors (1st to 3rd floors) to the wooden exterior of the upper floors (2nd to 4th floors), it is attempted to avoid the awkward appearance of the RC group being exposed to wooden structures. And it is also attempted to reflect the wooden shape and design elements through the elevation elements such as horizontal windows, corner windows, picture frames, and vertical slits. 3) In environmental control and facilities, it is attempted to smooth the ventilation of the building by forming a vertical upward airflow from the dark space of the low floor to the positive of the upper floor. This doubles the effect through a vertical rise of cold air generated in a narrow alleyway, piloti parking lot, and the various voids. In addition to the Daecheong and Numaru(loft) of Han ok, the rooftop yard, the terrace, and the balcony, horizontal natural ventilation is generated through divided doors and transom windows.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.17 no.3
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• pp.155-165
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• 2024

A hybrid thin film was prepared by doping reduced graphene oxide (rGO) into a sol-gel solution mixed with aluminum, titanium, and strontium using a brush coating method. The annealing temperature was carried out at 160, 260, and 360℃, and the difference in oxidation reaction was observed. The sol-gel solution created during the membrane manufacturing process generates a contractile force due to the shear stress of the brush bristles, forming a microgroove structure. This structure was confirmed through scanning electron microscopy analysis, and the presence of rGO was clearly revealed. As the annealing temperature increases, the oxidation and reduction reactions on the thin film surface become more active, so the intensity of the surface mixture increases. Moreover, the electro-optical properties were stabilized and improved by increasing the intensity of the mixtures. Likewise, the voltage-capacitance values are also significantly improved. Lastly, the transmittance measurement showed that it was suitable for liquid crystal display application.

• Particle and aerosol research
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• v.6 no.2
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• pp.61-67
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• 2010

In this paper, we describe a new method to form polymer thin films, in which carbon nanotubes (CNTs) are homogeneously distributed so that they can strengthen the mechanical property of resulting polymer film. To do so, we first homogeneously mixed CNTs with polymer in a DMF solvent. With the assistance of ultrasonic nebulizer, the polymer/CNT solution was then aerosolized into micro-sized droplets and finally turned into solidified polymer/CNT composite particles by gas-phase drying process. As the results of SEM and TEM analysis, CNTs were found to be homogeneously immobilized in the polymer matrix particles due to rapid drying process in the gas phase. For comparison purpose, (i) the polymer/CNTs composite particles prepared by aerosol processing method and (ii) polymer/CNTs sheets prepared by simple solution-evaporation method were employed to form polymer/CNTs composite thin films using a hot press. As the result, the aerosol processing of composite particles was found to be a much more effective method to form homogeneously distributed-CNTs in the polymer matrix thin film.

• Korean Institute of Interior Design Journal
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• v.20 no.2
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• pp.47-54
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• 2011

In Alvar Aalto's designs, important factors of complex building designs including Wolfsburg cultural center are that they insinuate how to develop each architectural type and how to combine them in a building. The humane and physical background studies, and wholistic and systematic approaches are adopted to fulfill research purposes. Comparison with other buildings are necessary to reveal true meaning of this building. The result of analysis show characteristics of Wolfsburg cultural center as follows; hybrid composition of mass and elevation, spatial effect using level difference and light, massing variation of roof, and creating space for various activities. Wolfsburg cultural center designed in late 1950s has greatly affected Aalto's later works through various architectural experiments because it is the first cultural complex project that combined various architectural types. Especially library in the cultural center has shown transitional characteristics to famous fan-type libraries of 1960s while maintained features about Viipuri library. Wolfsburg cultural center act as an another type which present new principles of clustering, massing and exterior design. Its true meaning lies in forming a humanizing place beyond spatial configuration.

• Journal of Biomedical Engineering Research
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• v.26 no.5
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• pp.319-330
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• 2005

Over the past few decades, much effort has been made to improve the mechanical and biological performance of HA, in order to extend its range of applications. As a major inorganic component of human hard tissues, hydroxyapatite bioceramic is regarded as being one of the most biocompatible materials. Numerous in vitro and in vivo studies have confirmed its excellent bioactivity, osteoconductivity and bone forming ability. However, because of its poor mechanical properties, its use in hard tissue applications has been restricted to those areas in which it can be used in the form of small sized powders/granules or in the non-load bearing sites. A number of researchers have focused on improving the mechanical and biological performance of HA, as well as on the formulation of hybrid and composite systems in order to extend its range of applications. In this article, we reviewed our recent works on HA-based biomaterials; i) the strengthening of HA with ceramic oxides, ii) HA-based bioactive coatings on metallic implants, iii) HA-based porous scaffolds and iv) HA-polymer hybrids/composites.

• Proceedings of the Polymer Society of Korea Conference
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• 2006.10a
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• pp.370-370
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• 2006

Thermoplastic elastomer gels, which has molecular networks composed of a microphase-separated multiblock copolymer swollen to a large extent by a low volatility mid-block selective solvent such as white oil have various applications. In this particular study, the effect of several network-forming nanoscale fillers such as two different graphite particles and carbon nanotube on the properties of TPE gels prepared from a microphaseordered poly[styrene-b-(ethylene-co-butylene)-b-styrene] (SEBS) triblock copolymer with an EB compatible white oil was studied. The linear viscoelastic behavior, sol-gel transition, x-ray diffraction and mechanical properties were discussed. The properties of thermoplastic elastomer gels hybrid with graphite prepared by mixing Poly(styrene-b-ethylene-co-butylene)-b-styrene) with paraffin oil and different amount of expandable graphite were found to increase the mechanical properties at only lower graphite concentration but tends to decrease when paraffin oil/SEBS ratio is lower. The gelation temperature is the same for all TPE gels with different amounts of graphite. Both storage (G') modulus loss (G") modulus of TPE gels slightly increase with addition of graphite.

• Transactions of Materials Processing
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• v.22 no.3
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• pp.133-142
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• 2013

Recently, electric vehicles and hybrid cars are being promoted as alternatives to reduce automobile emissions. Generally, thin sheet materials such as aluminum alloy AA300X and cold-rolled steel sheet such as JIS-G-3141 are used for the container for the lithium-ion secondary batteries. In this study, a multi-stage deep drawing process is used to produce a rectangular cup from thin stainless steel sheet material, SUS409L, with an initial blank thickness of 0.4mm for the battery container application. Numerical simulations of the first through the fifth stages for the multi-stage deep drawing with thin SUS409L sheet were conducted using LS-Dyna3D Implicit/Explicit. Special consideration was given to the deformation characteristics due to the normal anisotropy of the sheet material. The numerical simulations were conducted with both isotropic properties and the anisotropic properties of the initial blank material. An unexpected forming failure, barreling in the bottom region of the deep drawn rectangular cup, was observed. This failure mode can be avoided by additional ironing thickness control during the process.

• Fibers and Polymers
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• v.1 no.1
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• pp.32-36
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• 2000

An alternative strategy to .educe the percolation threshold of carbon black (CB) in polymer blends was investigated using random copolymer ternary blends of polystyrene (PS), poly(methyl methacrylate)(PMMA), and a styrene-methyl methacrylate random copolymer (SMMA). The target morphology was to selectively locate CB particles in the encapsulating layer of SMMA during melt mixing. The CB used in this study is BP-2000 from Cabot and has a strong selective affinity to PS. Even when the CB was premixed with SMMA, it moves to the PS phase during the melt mixing. However, we also observed the CB particles located at the interface between SMMA and PS phases. Through this study it is found that the interaction between polymers and CB particles is critical for selectively localizing CB particles in multi-component polymer blends. Although appropriate processing condition may retard the movement of CB particles to the polymer phase with affinity, it cannot prevent it completely and locate them to the SMMA phase, which is not thermodynamically favored. To locate CB particles in an encapsulating layer of ternary polymer blends, first of all, polymers forming it should have selective affinity to CB.