• Biomedical Science Letters
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• v.11 no.1
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• pp.51-56
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• 2005

The detection of high-risk human papilloma virus (HPV) allows us to predict the presence and future development of cervical intraepitheliallesion. In this study, we compared Hybrid Capture II and DNA chip methods for detection of HPV in cervical swab samples. And we evaluated the clinical efficacy and diagnostic performance of HPV DNA chip and Hybrid Capture II for detecting HPV in cervical neoplastic lesions. Seventy four patients were classified into three groups according to their histologic diagnosis: Group I (nonspecific chronic cervicitis), Group II (low-grade squamous intraepithelial lesion (SIL); koilocytosis, and mild dysplasia), and Group III (high-grade SIL;, moderate, severe dysplasia and in situ carcinoma). Cytologic diagnosis were based on the Bethesda System. Hybrid Capture II and DNA chip methods were performed to detect HPV. In 41 of the 74 cervical samples $(55.4\%)$, HPV DNAs were detected by Hybrid Capture II. In Group III, HPV-positive cases were detected in 15 $(20.3\%)$ of 74 patients by Hybrid Capture II. 25 patients with ASCUS cytology were histopathologically examined: 9 cases $(36\%)$ were Group II. In 18 patients with low-grade SIL cytology, 13 cases $(72.2\%)$ were Group II and 3 cases $(16.7\%)$ were Group III. 12 cases $(92.3\%)$ were Group ill of 13 patients with high-grade SIL cytology. The sensitivity of each test was $82\%$ in Hybrid Capture II and $53.9\%$ in DNA chip test. And the specificity was $74.3\%,\;85.7\%$ in Hybrid Capture II and DNA chip. In conclusion, Hybrid Capture II test is more sensitive than DNA chip in detecting women with cervical neoplastic lesions. Especially, in diagnosing of ASCUS, Hybrid Capture II test is more sensitive. Therefore, Hybrid Capture II test for cancer-associated HPV DNA is a viable option in the management of women with ASCUS.

• Journal of Korean Society for Atmospheric Environment
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• v.30 no.3
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• pp.261-269
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• 2014

This study focused on the performance of the newly developed hybrid filter system to capture fine particulate matter and mercury compounds in a coal-fired power plant. The hybrid filter system combining bag-filter and electrostatic precipitator had been developed to remove fine particulate matter. However, it would have a good performance to control mercury compounds as well. In Hybrid filter capture system, the total removal efficiency of total mercury compounds consisting of particulate mercury ($Hg_p$), oxidized mercury ($Hg^{2+}$), and elemental mercury ($Hg^0$) was 66.2%. The speciation of mercury compounds at inlet and outlet of Hybrid filter capture system were 1.3% and 0% of $Hg_p$, 85.2% and 68.1% of $Hg^0$, and 13.5% and 31.9% of $Hg^{2+}$, respectively. In hybrid filter capture system injected with 100% of flue-gas, the removal efficiency of total mercury was calculated to increase to 93.5%.

• Transactions of the Korean hydrogen and new energy society
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• v.28 no.1
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• pp.30-39
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• 2017

Interests in fuel cell based power generation systems are on the steady rise owing to various advantages such as high efficiency, ultra low emission, and potential to achieve a very high efficiency by a synergistic combination with conventional heat engines. In this study, the performance of a hybrid system which combined a molten carbonate fuel cell (MCFC) and an indirectly fired micro gas turbine adopting carbon dioxide capture technologies was predicted. Commercialized 2.5 MW class MCFC system was used as the based system so that the result of this study could reflect practicality. Three types of ambient pressure hybrid systems were devised: one adopting post-combustion capture and two adopting oxy-combustion capture. One of the oxy-combustion based system is configured as a semi-closed type, while the other is an open cycle type. The post-combustion based system exhibited higher net power output and efficiency than the oxy-combustion based systems. However, the semi-closed system using oxy-combustion has the advantage of capturing almost all carbon dioxide.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.11
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• pp.4465-4466
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• 2014

HPV type-specific detection may promote cervical screening program and vaccination development worldwide. We conduct a study comparing HPV Hybrid capture II (HC II) Test and Hybribio GenoArray test, a newly developed HPV type-specific assay, in patients with cervical epithelial neoplasm. Results showed a good concordance in cervical HPV detection between two tests (kappa value 0.80, p<0.05, McNemar test). Our study may promote utilization of type-specific HPV detection that is helpful for cervical cancer screening and vaccination.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.2
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• pp.903-907
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• 2013

Primary screening by HPV DNA testing is an effective method for reducing cervical cancer and has proven more sensitive than cytology. To advance this approach, many molecular methods have been developed. Hybrid capture 2 provides semi-quantitative results in ratios of relative light units and positive cutoff values (RLU/PC). Twenty-five thousand and five patients were included in this study to analyze the correlation between the ratio of RLU/PC and stage of cervical dysplasia. The results show that the RLU/PC ratios ranged from 0-3500 while almost normal cases, ASC-US and ASC-H, had values below 200. Of those samples negative for cytology markers, 94.6% were normal and their RLU/PC ratios were less than 4. With an RLU/PC ratio greater than 4 and less than or equal to 300, the percentages in all age groups were normal 53.6%, LSIL 20.2%, ASC-US 17.2%, HSIL 6.13%, ASC-H 2.72%, and AGC 0.11%, respectively. In contrast, 64.0% of samples with a RLU/PC ratio greater than 300 and less than or equal to 3500 were LSIL. These results should contribute to cost effective cervical cancer management strategies. Further studies of associations with particular HPV genotypes would be useful to predict the risk of progression to cancer.

• The Korean Journal of Cytopathology
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• v.19 no.2
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• pp.119-125
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• 2008

This study was performed to compare the efficacy between a DNA chip method and a Hybrid-Capture II assay (HC-II) for detecting human papillomavirus in patients with intraepithelial lesions of the uterine cervix. From May, 2005, to June, 2006, 192 patients with abnormal colposcopic findings received cervical cytology, HC-II and HPV DNA chip tests, and colposcopic biopsy or conization. We compared the results of HC-II and HPV DNA chip in conjunction with liquid based cervical cytology (LBCC) and confirmed the results of biopsy or conization. The sensitivity of the HPV DNA chip test was higher than HC-II or LBCC. The HPV DNA chip in conjunction with LBCC showed higher sensitivity than any single method and higher sensitivity than HC-II with LBCC. We confirmed that the HPV DNA chip test was more sensitive for detecting HPV in cervical lesions than HC-II, and that it would provide more useful clinical information about HPV type and its multiple infections.

• Nuclear Engineering and Technology
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• v.55 no.5
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• pp.1863-1870
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• 2023

Molybdenum-99 (⁹⁹Mo) is used for preparing Technetium-99 m (^99mTc), which is the most widely used isotope in nuclear medicine. In this work, a study for ⁹⁹Mo production based on a high-power electron accelerator has been performed as an alternative approach to produce ^99mTc. In this study, Monte Carlo MCNPX2.6 code has been used to examine a novel idea of simultaneous hybrid production of ⁹⁹Mo via both photoneutron and neutron capture reactions using an electron accelerator in heavy water tank. It is expected that this conceptual design including an arrangement of metallic plates of ¹⁰⁰Mo and ⁹⁸Mo produces total activity of 97.5 Ci at the end of 20-h continuous e-beam irradiation (30 MeV, 10 mA).

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.1
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• pp.21-27
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• 2016

Global warming due to greenhouse gas emissions is considered as a major problem worldwide, and many countries are making great efforts to reduce carbon dioxide emissions. Many technologies in post-combustion, pre-combustion and oxy-fuel combustion $CO_2$ capture have been developed. Among them, a hybrid pre-combustion $CO_2$ capture system of a water gas shift (WGS) reactor and a membrane gas separation unit was investigated. The 2 stage WGS reactor integrated high temperature shift (HTS) with a low temperature shift (LTS) was used to obtain a higher CO conversion rate. A Pd/Cu dense metal membrane was used to separate $H_2$ from $CO_2$ selectively. The performance of the hybrid system in terms of CO conversion and $H_2$ separation was evaluated using a 65% CO, 30 % $H_2$ and 5% $CO_2$ gas mixture for applications to pre-combustion $CO_2$ capture. The experiments were carried out over the range of WGS temperatures ($200-400^{\circ}C$), WGS pressures (0-20bar), Steam/Carbon (S/C) ratios (2.5-5) in a feed gas flow rate of 1 L/min. A very high CO conversion rate of 99.5% was achieved with the HTS-LTS 2 stage water gas shift reactor, and 83% $CO_2$ was concentrated in the retentate using the Pd/Cu membrane.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.5
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• pp.2063-2068
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• 2012

Aim: Liquid-based cytology is the most often used method for cervical cancer screening, but it is relatively insensitive and frequently gives equivocal results. Used as a complementary procedure, the high-risk human papillomavirus (HPV) DNA test is highly sensitive but not very specific. The human telomerase RNA gene (TERC) is the most often amplified oncogene that is observed in cervical precancerous lesions. We assessed genomic amplification of TERC in liquid-based cytological specimens to explore the optimal strategy of using this for cervical cancer screening. Methods: Six hundred and seventy-one residual cytological specimens were obtained from outpatients aged 25 to 64 years. The specimens were evaluated by the Digene Hybrid Capture 2 (HC2) HPV DNA test and fluorescence in situ hybridization (FISH) with a chromosome probe to TERC (3q26). Colposcopic examination and histological evaluation were performed where indicated. Results: The TERC positive rate was higher in the CIN2+ (CIN2, CIN3 and SCC) group than in the normal and CIN 1 groups (90.0% vs. 10.4%, p < 0.01). In comparison with the HC2 HPV DNA test, the TERC amplification test had lower sensitivity but higher specificity (90.0% vs. 100.0%, 89.6% vs. 44.0%, respectively). TERC amplification test used in conjunction with the HC2 HPV DNA test showed a combination of 90.0% sensitivity and 92.2% specificity. Conclusion: The TERC amplification test can be used to diagnose cervical precancerous lesions. TERC and HPV DNA co-testing shows an optimal combination of sensitivity and specificity for cervical cancer screening.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.2
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• pp.861-865
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• 2014

Background: High risk HPV (HR-HPV) testing has been recommended as an effective tool along with cytology screening in identification of cervical intraepithelial lesions (CINs) and prevention of their progress towards invasive cervical cancer. The aim of this study was to assess the HR-HPV DNA status by Hybrid Capture 2 (HC2) assay in healthy asymptomatic women of North-East India. Materials and Methods: This study examined cervical cell samples of forty three (n=43) healthy women by HC2 assay. A High Risk HPV DNA kit (Qiagen) was used which can detect 13 high risk HPV types: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68. Results: The mean relative light units (RLU) for samples was in the range of 141-5, 94, 619. HR-HPV DNA was confirmed in 16% (7/43) of participant women samples. Among demographic and clinical parameters, menstrual irregularity (p=0.039) and infection history (p=0.028) has shown statistically significant differences between the HR-HPV-positive and negative groups. In the HR-HPV positive group, two women were confirmed for CINs after colposcopy and histopathologic examination. Conclusions: We suggest that there may be an association between irregular menstruation and infection history of the urogenital tract with HR-HPV DNA prevalence in North-East Indian asymptomatic women. HC2 assay can be a valuable tool for HR-HPV screening.