• Molecules and Cells
• /
• v.20 no.1
• /
• pp.83-89
• /
• 2005

HtrA2/Omi is a mammalian mitochondrial serine protease homologous to the E. coli HtrA/DegP gene products. Recently, HtrA2/Omi was found to have a dual role in mammalian cells, acting as an apoptosis-inducing protein and being involved in maintenance of mitochondrial homeostasis. By screening a human brain cDNA library with $A{\beta}$ peptide as bait in a yeast two-hybrid system, we identified HtrA2/Omi as a binding partner of $A{\beta}$ peptide. The interaction between $A{\beta}$ peptide and HtrA2/Omi was confirmed by an immunoblot binding assay. The possible involvement of HtrA2/Omi in $A{\beta}$ peptide metabolism was investigated. In vitro peptide cleavage assays showed that HtrA2/Omi did not directly promote the production of $A{\beta}$ peptide at the ${\beta}/{\gamma}$-secretase level, or the degradation of $A{\beta}$ peptide. However, overexpression of HtrA2/Omi in K269 cells decreased the production of $A{\beta}40$ and $A{\beta}42$ by up to 30%. These results rule out the involvement of HtrA2/Omi in the etiology of Alzheimer's disease. However, the fact that overexpression of HtrA2/Omi reduces the generation of $A{\beta}40$ and $A{\beta}42$ suggests that it may play some positive role in mammalian cells.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.14 no.3
• /
• pp.715-722
• /
• 2010

This paper describes a hardware design of hash function processor which implements Korean Hash Algorithm Standard HAS-160. The HAS-160 processor compresses a message with arbitrary lengths into a hash code with a fixed length of 160-bit. To achieve high-speed operation with small-area, arithmetic operation for step-operation is implemented by using a hybrid structure of 5:3 and 3:2 carry-save adders and carry-select adder. It computes a 160-bit hash code from a message block of 512 bits in 82 clock cycles, and has 312 Mbps throughput at 50 MHz@3.3-V clock frequency. The designed HAS-160 processor is verified by FPGA implementation, and it has 17,600 gates on a layout area of about $1\;mm^2$ using a 0.35-${\mu}m$ CMOS cell library.

• Proceedings of the Korean Society of Developmental Biology Conference
• /
• 2003.10a
• /
• pp.121-121
• /
• 2003

The standard protocol for the production of transgenic mouse from ES-injected embryo has to process via chimera producing and several times breeding steps, In contrast, tetraploid-ES cell complementation method allows the immediate generation of targeted murine mutants from genetically modified ES cell clones. The advantage of this advanced technique is a simple and efficient without chimeric intermediates. Recently, this method has been significantly improved through the discovery that ES cells derived from hybrid strains support the development of viable ES mice more efficiently than inbred ES cells do. Therefore, the objective of this study was to generate transgenic mice overexpressing human resistin gene by using tetrapioid-ES cell complementation method. Human resistin gene was amplified from human fetal liver cDNA library by PCR and cloned into pCR 2.1 TOPO T-vector and constructed in pCMV-Tag4C vector. Human resistin mammalian expression plasmid was transfected into D3-GL ES cells by lipofectamine 2000, and then after 8~10 days of transfection, the human resistin-expressing cells were selected with G418. In order to produce tetraploid embryos, blastomeres of diploid embryos at the two-cell stage were fused with two times of electric pulse using 60 V 30 $\mu$sec. (fusion rate : 93.5%) and cultured upto the blastocyst stage (development rate : 94.6%). The 15~20 previously G418-selected ES cells were injected into tetraploid blastocysts, and then transferred into the uterus of E2.5d pseudopregnant recipient mice. To investigate the gestation progress, two El9.5d fetus were recovered by Casarean section and one fetus was confirmed to contain human resistin gene by genomic DNA-PCR. Therefore, this finding demonstrates that tetraploid-ES mouse technology can be considered as a useful tool to produce transgenic mouse for the rapid analysis of gene function in vivo.

• The Korean Journal of Physiology
• /
• v.29 no.2
• /
• pp.301-307
• /
• 1995

To explore electrophysiological properties of the ${\delta}-Opioid$ receptors artificially expressed in the mammalian cell, effect of an opioid agonist DPDPE $(1\;{\mu}M)$ on the voltage-sensitive outward currents was examined in the HEK293 (human embryonic kidney) cells transfected with ${\delta}-Opioid$ receptor cDNA cloned from NG-108-15 $(neuroblastoma\;{\times}\;glioma\;hybrid)$ cDNA library. Also studied were effects of 8-bromo-cyclic AMP and naloxone on DPDPE-induced changes in the voltage sensitive outward current. The voltage sensitive outward currents were recorded using perforated patch technique at room temperature. In the non-transformed HEK293 cells, DPDPE did not alter voltage sensitive outward current, indicating that no native ${\delta}-Opioid$ receptor had been developed. However, $(1\;{\mu}M)$ DPDPE remarkably increased the voltage sensitive outward current in the transformed HEK293 cells. The increment in voltage sensitive outward current peaked in $7{\sim}10\;minutes$ after DPDPE application, and the maximum DPDPE-activated outward current $(313.1{\pm}12.3\;pA)$ was recorded when the membrane potential was depolarized to +70mv. Following pretreatment of the transformed HEK293 cells with 1 mM 8-bromo-cyclic AMP, DPDPE failed to increase the voltage sensitive outward currents. On the other hand, naloxone completely abolished DPDPE-activated voltage sensitive outward current in the transformed HEK293 cells. The results of present study suggest that in the transformed HEK293 cells an activation of the ${\delta}-Opioid$ receptors by an opioid agonist DPDPE increases the voltage-sensitive potassium current as a result of decrement in cyclic AMP level.

• Journal of Microbiology and Biotechnology
• /
• v.11 no.6
• /
• pp.1115-1119
• /
• 2001

Eight Escherichia coli cells with aerobic growth deflects were isolated by the insertion of ${\lambda}placMu53$, a hybrid bacteriophage of ${\lambda}$ and Mu, which created transcriptional fusion to lacZY. Two of these mutants, CLIO and CLl2, were irradiated with UV to obtain specialized transducing phages. The phages that took out the neighboring chromosomal DNA of the related gene responsible for deflective aerobic growth were identified. The in vivo cloned chromosomal sequence revealed that the mutated gene of CLIO was located at min 34.5 on the Escherichia coli linkage map and 1,599,515 on the physical map. The physical map indicated that there were 7 cistrons in the operon. We named this operon oxg10. The promoter sequence of oxg10 exhibited a possible binding site far SoxS, a transcriptional regulator that activates the transcription of various SoxRS regulon genes. Transferring the oxg10:: ${\lambda}placMu53$ mutation into the wild-type strain, RZ4500, resulted in the inhibition of normal aerobic growth, while the salute mutation in strain MO inhibited aerobic cell growth completely. The full operon sequences of oxg10 were cloned from the Excherichia coli genomic library. The mutated gene of CLl2 was identified to be a sucA gene encoding the ${\alpha}$-ketoglutarate dehydrogenase El component in the TCA cycle.

• Spatial Information Research
• /
• v.22 no.2
• /
• pp.63-71
• /
• 2014

Ocean leisure sports have recently emerged as one of so-called blue ocean industries. They are sensitive to diverse environmental conditions such as current, temperature, and salinity, which can increase needs of forecasting data as well as in-situ observations for the ocean. In this context, a Web-based geovisualization system for coastal information produced by model forecasts was implemented for use in supporting various ocean activities. First, FVCOM(Finite Volume Coastal Ocean Model) was selected as a forecasting model, and its data was preprocessed by a spatial interpolation and sampling library. The interpolated raster data for water surface elevation, temperature, and salinity were stored in image files, and the vector data for currents including speed and direction were imported into a distributed DBMS(Database Management System). Web services in REST(Representational State Transfer) API(Application Programming Interface) were composed using Spring Framework and integrated with desktop and mobile applications developed on the basis of hybrid structure, which can realize a cross-platform environment for geovisualization.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.14 no.1
• /
• pp.205-212
• /
• 2010

This paper describes a core generator (FCore_Gen) which generates Verilog-HDL models of 640 different FFT/IFFT cores with selected parameter value for OFDM-based communication systems. The generated FFT/IFFT cores are based on in-place single memory architecture and use a hybrid structure of radix-4 and radix-2 DIF algorithm to accommodate various FFT lengths. To achieve both memory reduction and the improved SQNR, a conditional scaling technique is adopted, which conditionally scales the intermediate results of each computational stage. The cores synthesized with a $0.35-{\mu}m$ CMOS standard cell library can operate with 75-MHz@3.3-V, and a 8,192-point FFT can be computed m $762.7-{\mu}s$, thus the cores satisfy the specifications of wireless LAN, DMB, and DVB systems.

• The Plant Pathology Journal
• /
• v.30 no.1
• /
• pp.58-67
• /
• 2014

The multifunctional triple gene block protein 1 (TGB1) of the Potexvirus Alternanthera mosaic virus (AltMV) has been reported to have silencing suppressor, cell-to-cell movement, and helicase functions. Yeast two hybrid screening using an Arabidopsis thaliana cDNA library with TGB1 as bait, and co-purification with TGB1 inclusion bodies identified several host proteins which interact with AltMV TGB1. Host protein interactions with TGB1 were confirmed by biomolecular fluorescence complementation, which showed positive TGB1 interaction with mitochondrial ATP synthase delta' chain subunit (ATP synthase delta'), light harvesting chlorophyll-protein complex I subunit A4 (LHCA4), chlorophyll a/b binding protein 1 (LHB1B2), chloroplast-localized IscA-like protein (ATCPISCA), and chloroplast ${\beta}$-ATPase. However, chloroplast ${\beta}$-ATPase interacts only with $TGB1_{L88}$, and not with weak silencing suppressor $TGB1_{L88}$. This selective interaction indicates that chloroplast ${\beta}$-ATPase is not required for AltMV movement and replication; however, TRV silencing of chloroplast ${\beta}$-ATPase in Nicotiana benthamiana induced severe tissue necrosis when plants were infected by AltMV $TGB1_{L88}$ but not AltMV $TGB1_{L88}$, suggesting that ${\beta}$-ATPase selectively responded to $TGB1_{L88}$ to induce defense responses.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.29 no.5A
• /
• pp.554-565
• /
• 2004

In this paper, we implement single cycle and multi cycle adders. We can compare area and time by using the implemented adders. The size of adders is 64, 128, 256-bits. The architecture of hybrid adders is that the carry-out of small adder groups can be interconnected by utilizing n carry propagate unit. The size of small adder groups is selected in three formats - 4, 8, 16-bits. These adders were implemented with Verilog HDL with top-down methodology, and they were verified by behavioral model. The verified models were synthesized with a Samsung 0,35(um), 3.3(V) CMOS standard cell library while a using Synopsys Design Compiler. All adders were synthesized with group or ungroup. The optimized adder for a Crypto-processor included Smart Card IC is that a 64-bit RCA based on 16-bit CLA. All small adder groups in this optimized adder were synthesized with group. This adder can operate at a clock speed of 198 MHz and has about 961 gates. All adders can execute operations in this won case conditions of 2.7 V, 85 $^{\circ}C$.

• Journal of Microbiology and Biotechnology
• /
• v.12 no.5
• /
• pp.807-812
• /
• 2002

In order to analyze the cellular proteins which interact with core protein of hepatitis C virus (HCV), a yeast two-hybrid screening technique was employed. A carboxyl terminus truncated core protein, which contained amino acid residues from the 1st to 120th, was used as a bait to screen cellular proteins. The expression library prepared from HeLa cell was screened and 400 positive clones were selected. The 75 clones from the positive clones were sequenced and analyzed by undergoing the Blast search. Interestingly, 7 out of the 75 clones encoded E7 antigen of human papilloma virus (HPV). We studied in detail the Interaction between the truncated version of HCV core and E7 antigen in vitro. The core$_{120}$ protein expressed in chimeric form with G57 was able to bring down the E7 protein of HPV type 18 expressed in bacteria. It is therefore suggested that the core of HCV might affect the interaction between E7 and a normal cellular tumor suppressor, known as Rb protein.