• 제목/요약/키워드: Hwangtae

검색결과 7건 처리시간 0.019초

Isolation and Charaterization of Bioactive Peptides from Hwangtae (yellowish dried Alaska pollack) Protein Hydrolysate

  • Cho, San-Soon;Lee, Hyo-Ku;Yu, Chang-Yeon;Kim, Myong-Jo;Seong, Eun-Soo;Ghimire, Bimal Kumar;Son, Eun-Hwa;Choung, Myoung-Gun;Lim, Jung-Dae
    • Preventive Nutrition and Food Science
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    • 제13권3호
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    • pp.196-203
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    • 2008
  • Hwangtae, dried Alaska pollack, is a major storage product in the fish processing industry. Hwangtae is prepared by removing the internal organs and drying outdoors during the cold witner months by allowing it to thaw during the daytime and re-freeze at night under sub-zero ($-10^{\circ}C$) conditions and gradually dry from December until the next April for around 5 months from Myungtae. In this study, ground Hwangtae was hydrolyzed using two proteolytic enzymes (pepsin and alcalase) which produced five soluble active peptides from Hwangtae (yellowish dried Pollack, Theragra chalcogramma) protein. Two different peptides with strong antioxidative activity were isolated from the hydrolysate using consecutive chromatographic methods of Sephadex G-25 gel, ion-exchange chromatography on a Sepharose-Sephadex C-25 gel, and high-performance liquid chromatography. The isolated peptides, APO1 and APO2, were composed of 16 and 13 amino acid residues, respectively. Both peptides contained a Gly residue at the C-terminus and the repeating motif Gly-Pro-Hyp. The peptide with a molecular weight less than 1,000 Daltons (APACE) obtained from enzymatic hydrolysates of Hwangtae exhibited the highest ACE inhibitory activity. The APACE peptides was composed of 4 amino acid residues (Gly-Leu-Leu-Pro). These results suggest that Hwangtae hydrolysates could be a good source of peptides with ACE inhibitory activity. Biochemical analysis indicated that two 70 kDa peptides (APG1 and APG2) isolated from the hydrolysate had gelatinoytic activity, which was shown to be a calcium dependent protease type as showed by gelatin SDS PAGE.

Physicochemical Properties of Isolated Peptides from Hwangtae (yellowish dried pollack) Protein Hydrolysate

  • Cho, San-Soon;Lee, Hyo-Ku;Han, Chi-Won;Seong, Eun-Soo;Yu, Chang-Yeon;Kim, Myong-Jo;Kim, Na-Young;Kang, Wie-Soo;Ko, Sang-Hoon;Son, Eun-Hwa;Choung, Myoung-Gun;Lim, Jung-Dae
    • Preventive Nutrition and Food Science
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    • 제13권3호
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    • pp.204-211
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    • 2008
  • Fish protein hydrolysates (FPHs) with different degrees of hydrolysis by treatment with alcalase, pronase, flavourzyme and trypsin and isolated peptide were prepared from Hwangtae (yellow dried pollack, Theragra chalcogramma). Hwangtae protein hydrolysate was fractionated according to the molecular weight into six major types of APO1 (1.3 kDa), APO2 (1 kDa), APO3 (<1 kDa), APACE (<1 kDa), APG1 (70 kDa) and APG2 (70 kDa) isolated from the hydrolysate using consecutive chromatographic methods. Soluble peptide were produced from Hwangtae and evaluated for their nutritional and functional properties. Some functional properties of FPHs were assessed and compared with those of egg albumin or the soybean protein. APO2 had the highest nitrogen solubility value (94.2%), emulsion capacity and emulsion stability of the Alaska Pollack peptide ranged from 12.4 to 39.5 (mL of oil per 200 mg of protein) and 44.0% to 77.5%, respectively. Highest and lowest fat adsorption values were observed for APG1 (9.9 mL of oil per gram of protein) and APO3 (3.8 mL of oil per gram of protein), respectively.

명태건조방법에 따른 갈변화 관련 물질의 변화 (Effects of Different Drying Methods on Fatty Acids, Free Amino Acids, and Browning of Dried Alaska Pollack)

  • 최희선;김종환;김재철
    • 한국식품영양과학회지
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    • 제36권9호
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    • pp.1182-1187
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    • 2007
  • 건조된 명태들은 전반적으로 다가불포화지방산이 감소하고 단일불포화지방산은 증가하는 경향을 보였다. 특히 주기적인 자연냉동과 해동을 반복하면서 건조된 명태(미숙성황태)를 장기간 숙성하여 완성한 황태의 지방산 조성은 북어나 미숙성황태와 비교해 볼 때, 원료시료(명태)간의 차이를 감안하더라도 특징적인 차이를 보였다. 총 유리아미노산 함량도 황태가 북어나 미숙성황태보다 낮게 나타나 불포화지방산과 유리아미노산의 반응에 의한 점진적인 갈변화 현상을 설명해 주는 것으로 판단된다. 황태의 수용성 갈변물질은 주로 숙성단계에서 많이 형성되는 것으로 추정되었다.

Comparative Analysis of the Physicochemical Properties of Sun-dried and Natural Cyclic Freeze-Thaw Dried Alaska Pollack

  • Kim, Jong-Hwan;Choi, Hee-Sun;Lee, Sang-Hyun;Hong, Jeong-Hwa;Kim, Jae-Cherl
    • Food Science and Biotechnology
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    • 제16권4호
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    • pp.520-525
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    • 2007
  • The physicochemical properties of sun-dried and cyclic freeze-thaw dried Alaska pollack were analyzed to compare the 2 drying processes. The moisture content and water activity of sun-dried Alaska pollack were higher than cyclic freeze-thaw dried and 1 year-aged cyclic freeze-thaw dried Alaska pollack (hwangtae). The relatively low temperatures used in cyclic freeze-thaw drying retards lipid oxidation compared to sun drying based on the acid and peroxide values, and the levels of thiobarbituric acid-reactive substances (TBARS) in the dried fish. The water holding capacity of cyclic freeze-thaw dried Alaska pollack aged for 1 year (hwangtae) under ambient conditions at the drying location was higher than that of sun-dried Alaska pollack. The swelling of myofibrilar filaments during cyclic freeze-thaw drying may be responsible for the softening of the dried muscle protein. Aging the cyclic freeze-thaw dried Alaska pollack for 1 year contributed to an increased yellowish color of the hwangtae.

명태 건조과정에서 일어나는 수분과 수분활성도 변화가 명태건조품의 물성에 미치는 영향 (Drying of Alaska Pollack in Controlled Conditions to Identify Major Factors for Textural Properties of Hwangtae)

  • 하주엽;이상현;정경진;조영득;김재철
    • 한국식품영양과학회지
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    • 제39권12호
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    • pp.1903-1907
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    • 2010
  • 명태의 건조경향은 초기의 평형상태가 뚜렷하지 않은 과정을 제외하면, 일반적인 식품건조형태를 따르는 것으로 보인다. 따라서 시간이 지날수록 건조속도는 어육과 외기의 수분농도차이가 줄어듦에 따른 결과뿐만 아니라, 표면 건조에 의한 고화현상에 의해 수분이동이 억제됨에 따라 느려지게 된다. 명태의 부위별 건조속도는 잘 알려진 바와 같이 머리 부분이 몸통부분보다 빨랐다. 건조가 진행될수록 어육표면과 중심부의 수분함량과 그에 따른 수분활성도의 차이는 증가함으로써 어육내부의 수분이동이 잘 일어나지 않아 전체적인 건조속도의 저하를 나타내었다. 주어진 외기의 건조조건(온도, 습도, 풍속)에서 초기의 높은 건조속도에 의한 어육 표면응고로 인해 건조개시 수 시간 이내에 어피(피부)와 어피 바로밑부분의 어육과 어체 내부 어육의 수분함량 차이가 급격히 일어나는 것을 알 수 있었다. 황태 제조과정의 전단계인 건조과정에서 건조가 시작된 뒤 주기적으로 수분활성도의 차이가 급격히 일어나는 시간(지점)은 황태 특유의 조직감을 형성하기 위해 건조과정에서 조직의 고화를 방지함으로써 일정한 건조속도를 유지하기 위해 주기적으로 수분을 공급해 주어야 하는 시점임을 제시해 준다.

시판 어류 단순가공품의 세균학적 및 화학적 위해요소 분석 (Bacteriological and Chemical Hazard Analysis in Commercial Fish Products Minimally Processed)

  • 김현정;이동수;김일회;김영목;신일식
    • 한국수산과학회지
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    • 제52권1호
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    • pp.19-26
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    • 2019
  • The objective of this study was to analyze bacteriological and chemical hazards in minimally processed commercial fish products, including Hwangtae (freeze-dried pollock), dried anchovy, fermented anchovy sauce, and salted and dried yellow croaker. Escherichia coli counts from all samples were below the regulation limits of the Korean Ministry of Food and Drug Safety Standards on Quality of Seafood and Seafood Products (Food Code). However, the food poisoning bacterium Staphylococcus aureus was detected at levels above $1.0{\times}10^2$ colony forming units (CFU)/g in Hwangtae, dried anchovy, and salted and dried yellow croaker, which are commonly ingested without heating and pose bacteriological hazards. The detection of S. aureus, an organism indicative of poor personal hygiene, which can be introduced by employees and multiply during distribution, indicates the necessity of improving the sanitary control of minimally processed commercial fish products. Histamine was not detected from dried anchovy or salted and dried yellow croaker, but was detected at some of the highest levels in fermented anchovy sauces. This result suggests that efforts to reduce the amount of histamine in fermented anchovy sauces are required.

사상체질에 근거한 노인의 한방약선죽 개발에 관한 연구 (Development of Oriental Medicinal Porridges Based on Sasang Constitutional Medicine for the Elderly)

  • 홍종숙;임현정
    • 한국식생활문화학회지
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    • 제36권6호
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    • pp.607-621
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    • 2021
  • The elderly population is rapidly increasing in South Korea, and interest in food development considering the Sasang constitution theory from oriental medicine and balanced nutrition is increasing. We developed the oriental medicinal porridges based on the Sasang constitution for the elderly. By Sasang constitutional medicine, Taeyangin has a large lung and small liver, and Soyangin has a large stomach and small kidney. Taeeumin has a small lung and large liver, and Soeumin has a small stomach and a large kidney. In this study, proper oriental medicine and food ingredients were identified, and a total of 12 oriental medicinal porridges were developed for 3 items by 4 Sasang constitution types. A single portion was developed based on about 600±66kcal, and the food ingredients were chopped. After cooking the menu developed based on the Sasang constitution, a sensory test was conducted. Five items, such as taste, appearance, aroma, texture, and overall preference, were evaluated on a 7-point scale. Sewage omegaenggul porridge among porridges for Taeyangin had the highest overall acceptance (6.17±0.7 points). Sukjihwang abalone black sesame porridge for Soyangin had the highest score(5.83±0.9 points). Sanyakyulmu hwangtae porridge for Taeeumin had the highest score(5.90±0.6 points). Ginseng chicken curry porridge among porridges for Soeumin had the highest overall preference in taste, appearance, aroma, texture, and overall acceptability (6.53±0.7 points). A limitation of this study was that the clinical trial could not be conducted on the elderly classified as Sasang constitution. In the future, the elderly will be able to have oriental medicinal foods according to the classification of Sasang constitution.