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Analysis of Heavy Metal Contamination and Shellfish Poisoning Toxins in Marketed Shellfishes (유통 패류의 중금속 및 패류독소 분석)

  • Kim, Jeong-Eun;Hwang, Seon-Il;Lee, Seong-Bong;Shin, Sang-Woon;Kwon, Hye-Jung;Lee, Ji-Yeon;Lee, Byoung-Hoon;Mo, A-Ra;Choi, Ok-Kyung
    • Journal of Food Hygiene and Safety
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    • v.37 no.3
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    • pp.159-165
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    • 2022
  • In this study, heavy metals (lead, cadmium, and mercury) and shellfish poisoning toxins (diarrhetic shellfish poisoning toxins, amnesic shellfish poisoning toxins) were investigated in a total of 104 shellfishes. According to the analysis of heavy metals, lead (Pb) was detected in the range of 0.0177-0.5709 mg/kg, cadmium (Cd) was detected in the range of 0.0226-1.4602 mg/kg, and mercury (Hg) was detected in the range of 0.0015-0.0327 mg/kg. Levels of Pb, Cd, and Hg were acceptable by Korean standards. Okadaic acid (OA) and dinophysistoxin-1 (DTX-1) were investigated for monitoring of diarrhetic shellfish poisoning toxins and OA and DTX-1 were not detected. As a result of monitoring of amnesic shellfish poisoning toxins, domoic acid was detected in 5 of 104 samples and detection ratio was 4.8%. The detection period was found as follows; 1 case in January, 1 case in February, 1 case in May, 2 cases in September. These showed that continuous monitoring for the management of shellfish poisoning toxins and heavy metals is required. In addition, this study can be used as reference data to strengthen managing heavy metals in fishery products.

Effects of Coir Substrate Application and Substrate Volume on the Growth and Yields of Strawberry in a Hydroponically Cultured System (딸기 수경재배에 코이어 배지 적용과 근권부 배지 용량이 생육 및 수확량에 미치는 영향)

  • Hwang, Jeongsu;Yun, Sungwook;Kwon, Jinkyung;Park, Minjung;Lee, Dongsoo;Lee, Heeju;Lee, Siyoung;Lee, Sanggyu;Hong, Youngsin
    • Journal of Bio-Environment Control
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    • v.31 no.3
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    • pp.163-169
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    • 2022
  • This study was conducted to examine an alternative cultivating method that uses coir substrates in a hydroponically cultured system. Three treatment conditions were applied with one-layer substrate (10 cm height) with a coir chip and dust ratio of 5:5 (Treatment A), two-layer coir substrate (20 cm height) with a coir chip and dust ratio of 5:5 (Treatment B), one-layer coir substrate (15 cm height) with a coir chip and dust ratio of 7:3 (Treatment C). The control condition was a plastic container filled with a coir chip and dust ratio of 5:5. Various criteria were measured and compared between the treatments and the control. The yield of strawberry was smaller in the control than in the treatments. No significant difference in growth characteristic was found in the height treatments of the coir substrates. The net photosynthetic rate of the treatments was 14.68-15.76 µmol CO2·m-2·s-1. This does not show a statistically significant difference. The root activity was better in treatment B and C than in treatment A and the control. The length and width of leaves were measured as 4.04-4.13 cm and 3.26-3.34 cm. These results are not statistically significant. The leaf length and width ratio was 1.27 in the control and 1.24 in the treatments. The findings show that no statistically significant benefit was found when utilizing coir substrates with different height treatments in the hydroponic culture system. However, the harvested fruit per plant weights 72.38 g in treatment A and 48.69 g in treatment C. The number of harvested fruit was least in treatment C in which a coir chip and dust ratio of 7:3 was applied. Therefore, further research is needed to examine how the chip and dust ratio in coir substrate affects growth characteristics.

Growth, Yield, and Leaf-macronutrient Content of Grafted Cherry Tomatoes as Influenced by Rootstocks in Semi-forcing Hydroponics (반촉성 수경재배시 대목에 따른 방울토마토 접목묘의 생육, 수량 및 엽 내 양분 함량)

  • Hyewon Lee;Hyo Bong Jeong;Jun Gu Lee;Indeok Hwang;Deok Ho Kwon;Yul Kyun Ahn
    • Journal of Bio-Environment Control
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    • v.32 no.1
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    • pp.40-47
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    • 2023
  • There are many different types of cultivation in tomatoes for year-round production. One of them, semi-forcing cultivation is characterized by growing seedlings in winter season. If grafted seedlings are used in winter season that energy cost can be reduced, because they have tolerance to cold stress. This study was conducted to analyze the rootstock performance by measuring the growth, yield, and leaf-macronutrient content of cherry tomatoes grown in semi-forcing hydroponics. Three domestic rootstocks 'HSF4', '21LM', '21A701', and a control cultivar 'B-blocking' were grafted onto jujube-shaped cherry tomato (Lycopersicon esculentum L.) commercial cultivar 'Nonari'. The total yield per plant with grafted cherry tomato '21A701' was 3,387g, which was 11%, 22% and 24% higher than the yield with 'B-blocking', non-grafted one and 'HSF4'. The stem diameter of '21A701' was thick with 8.26mm, whereas non-grafted one was thin with 7.23mm at 160 days after transplanting. The flowering position of '21LM' was 34% and 47% higher than the flowering position of 'B-blocking' and non-grafted one at 153 days after transplanting. The NO3-N concentration in petiole sap of '21LM' was the highest with 1,746mg·L-1 and non-grafted one and 'HSF4' were the lowest with 1,252mg·L-1 and 1,245mg·L-1 at 167 days after transplanting. The results indicated that rootstock/scion combinations in cherry tomatoes can affect the plant growth, yield, and the concentration of different NO3-N in leaves at the late growth stage. Both '21A701' and '21LM' have vigorous root system, which influence the growth and yield increased.

Investigation of Heavy Metal Migration from Food Contact Materials used for Food Delivery Using an Inductively Coupled Plasma-Mass Spectrometer

  • Chae-Yeon Hwang;Young-Jun Kim
    • Journal of Food Hygiene and Safety
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    • v.38 no.2
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    • pp.37-45
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    • 2023
  • The surge in food delivery systems during the coronavirus 2019 pandemic necessitated this study of heavy metal migration from food contact materials (FCMs). A total of 104 samples of FCMs, comprising 51 polypropylene (PP), 21 polyethylene (PE), and 32 polystyrene (PS) samples of six different types of FCMs (containers, covers, table utensils, cups, pouches, and wrappers) used for food delivery distributed in Korea, were collected and investigated for migration of three heavy metals (Pb, Cd, and As) using inductively coupled plasma-mass spectrometry (ICP-MS) to determine whether they complied with Korea's Standards and Specifications for Utensils, Containers, and Packages. Acetic acid (4%, v/v) was used as the food simulant, and tests were performed at 100℃ (in harsh conditions) for 30 min. Linearity of Pb, Cd, and As showed acceptable results with a coefficient of determination (R2) value of 0.9999. Limit of detection (LOD) and limit of quantification (LOQ) of Pb, Cd, and As were 0.001, 0.001, and 0.001 ㎍/L and 0.002, 0.003, and 0.003 ㎍/L, respectively. Accuracy and precision results complied with the criteria presented in the European Commission Joint Research Centre guidelines. The average concentration of Pb, Cd, and As migration detected in a total of 104 samples was 0.009-0.260 ㎍/L, which was very low compared with the migration specification set in the Standards and Specifications for Utensils, Containers, and Packages. The maximum level of Pb corresponded to 0.23% of the migration limit. There were no samples exceeding the limit. Thus, this study confirmed that the heavy metal contents of FCMs used for delivery food distributed in Korea were safely managed. The data from this study represent an invaluable source for science-based safety management of hazardous heavy metals migrating from FCMs used in the food delivery industry.

Protective Effects of Trifolium pratense L. Extract against H2O2-induced Oxidative Stress in HaCaT Keratinocytes (인간 피부각질세포에서 Hydrogen peroxide로 유도된 산화적 스트레스에 대한 붉은 토끼풀 추출물의 세포 보호 효과)

  • Mi Song Shin;You Kyeong Lee;Seo Young Choi;Ji Sun Hwang;Parkyong Song;Hyeon Cheal Park;Keun Ki Kim;Hong-Joo Son;Yu-Jin Kim;Kwang Min Lee
    • Journal of the Korean Applied Science and Technology
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    • v.40 no.2
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    • pp.223-232
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    • 2023
  • Oxidative stress plays a significant role in the pathogenesis of various skin conditions, resulting in cellular and tissue damage that can contribute to the development of skin tone unevenness, roughness and wrinkles. In this study, we found that Trifolium pratense L. extract (TE) attenuated oxidative-induced damage in HaCaT cells and elucidated the underlying molecular mechanism. Our finding demonstrated that TE effectively protected HaCaT cells against H2O2-induced cell death by inhibiting caspase-3 activation, downregulating Bax and upregulating Bcl-2, and attenuating the activation of three mitogen-activated protein kinases (MAPKs). Our results suggest that TE has remarkable cytoprotective properties against oxidative damage in HaCaT cells and could serve as a complementary or alternative approach to prevent and treat skin damage.

Monitoring of arsenic and arsenic species in fish collagen in Korea (국내 유통 어류 콜라겐의 총비소 및 비소화학종 함량 모니터링)

  • Yeo-Jae Shin;Mi-Ra Jang;Eun-Hee Kim;Yun-Hee Kim;Min-Jung Kim;Min-Jung Kim;Jae-Hoon Cha;Mi-Hyun Choi;Seok-Ju Cho;In-Sook Hwang;Yong-Seung Shin
    • Analytical Science and Technology
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    • v.36 no.3
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    • pp.135-142
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    • 2023
  • The total arsenic and 6 arsenic species were investigated in 56 fish collagen products using ICP-MS (Inductively coupled plasma-mass spectrometer) and HPLC-ICP-MS(High performance liquid chromatography-Inductively coupled plasma-mass spectrometer). The mean concentrations of total arsenic and arsenic species were 40.103±81.133 ㎍/kg (N.D.~586.686) and 30.070±50.378 ㎍/kg (N.D.~313.871), respectively. The mean concentration of inorganic arsenic was 24.610±32.706 ㎍/kg (N.D.~129.331), and the As(V) (Arsenate) was the most dominant. The standards and specifications of arsenic have not been established for fish collagen products. Our study presents that arsenic levels are relatively safe compared with not only previous studies but also domestic and international standards. However, in one sample, the total arsenic concentration was 586.686 ㎍/kg, showing the inorganic was 8.119 ㎍/kg, and the DMA was 305.752 ㎍/kg, which was high than the Canadian standard for organic arsenic. In conclusion, it is necessary to monitor arsenic levels consistently and establish standards and specifications of arsenic in fish collagen products to assure consumer safety.

Treatment Effect with Weekly Teriparatide in the Vertebral Compression Fractures in Patients with Severe Osteoporosis (심한 골다공증 환자에서 발생한 척추체 압박골절에 대한 주 단위 테리파라타이드(Teriparatide)의 투여 효과)

  • Hwang, Seok-Ha;Woo, Young-Kyun;Jeon, Ho-Seung;Suh, Seung-Pyo;Kim, Joo-Young;Kim, Jae-Nam
    • Journal of the Korean Orthopaedic Association
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    • v.54 no.6
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    • pp.528-536
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    • 2019
  • Purpose: This study examined the effects of a weekly teriparatide on the change in vertebral compression ratio, back pain, and vertebral fracture healing in osteoporosis patients with vertebral compression fractured induced by low energy trauma. Materials and Methods: From January 2016 to December 2017, 57 patients with severe osteoporotic vertebral fractures with a T score of -3.5 or less were included in this study. The changes in the vertebral compression ratio, visual analogue scale (VAS), Oswestry disability index (ODI) for at least 6 months were examined. The morphology of bone marrow edema and the presence of intervertebral cleft, osteocalcin, and N-terminal telopeptide (NTx) were also investigated. Results: The mean compression ratio was 20% in the experimental group (teripratide group) at 3 months, and 38% in the control group. A significant difference in the compression ratio of the vertebral body over time was observed (p<0.05; t-test). A comparison of the compression ratio of the vertebral body with the follow-up duration in each group showed no significant increase in the, compression (p=0.063) in the experimental group and a significant increase in the control group (p<0.05). The mean time to reach the plateau of the compression rate was one month in the experimental group and three months in the control group. The VAS score in the experimental and control group was 0.39 and 1.07 points, respectively. The ODI score in the experimental and control group was 33.72 and 39.52, respectively. At the last follow-up radiographs, there were no cases with an intervertebral cleft (0%) in the experimental group and 1 case (2.2%) in the control group. A significant difference in the osteocalcin level was observed between the injury and 6 months after the injury (p=0.003). In addition, there was no significant difference in the NTx level between the injury and 6 months after injury (p=0.960). Conclusion: In vertebral compression fractures patients with severe osteoporosis, a weekly teriparatide can promote the union of fractures, prevent further collapse of the vertebral body, and reduce the back pain faster.

Effect of Heavy Metals (As, Cr, Pb) on the Population Growth Rates of Marine Diatom, Skeletonema castatum (중금속(As, Cr, Pb)이 해산 규조류(Skeletonema costatum)의 개체군 성장률에 미치는 영향)

  • Lee, Ju-Wook;Ryu, Hyang-Mi;Heo, Seung;Jang, Soo-Jung;Lee, Ki-won;Hwang, Un-Ki
    • Journal of Marine Life Science
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    • v.2 no.1
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    • pp.20-26
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    • 2017
  • In this study, we evaluated the toxic effects of heavy metals (As, Cr and Pb) on the population growth rate (r) of the marine diatom, Skeletonema costatum. The r of the species was determined after 96 h (4 days) exposure to As (0, 0.31, 0.63, 1.25, 2.50 and 5.00 mg L-1), Cr (0, 0.16, 0.31, 0.63, 1.25 and 2.50 mg L-1) and Pb (0, 1.25, 2.50, 5.00, 10.00 and 20.00 mg L-1). It was observed that r in the control (absence of As, Cr and Pb) were greater than 0.04, however suddenly decreased with increased heavy metal concentrations. As, Cr and Pb reduced r in a dose-dependent manner and a significant reduction were occurred at concentration of greater than 0.31, 0.31 and 5.00 mg L-1, respectively. Based on the toxicity, the heavy metal were ranked as Cr>As>Pb, with EC50 (50% effective concentration) values of 0.80, 2.57 and 13.88 mg L-1, respectively. The lowest-observed-effective-concentration (LOEC) of r in exposed to As, Cr and Pb were 0.63, 0.63 and 10.00 mg L-1, respectively. Precisely, a concentration of greater than 0.63 mg L-1 of As, 0.63 mg L-1 of Cr and 10.00 mg L-1 of Pb in marine ecosystems induced toxic effect on the r of S. costatum. Based on our results, we suggested that the r of S. costatum might be a useful bio-indicator for the toxicity assessment of heavy metals on the producer in marine ecosystems.

Difference of perception of the duties of dental hygienist between dentists and dental hygiene students in an area (일개 지역 치과의사와 치위생학과 학생 간 치과위생사의 업무에 대한 인식의 차이)

  • Hwang, Soo-Jeong;Koong, Hwa-Soo;Lee, Sang-Hoon
    • Journal of Korean Academy of Dental Administration
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    • v.5 no.1
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    • pp.1-12
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    • 2017
  • Although dental hygienists have performed chair-side assisting and other dental cares as well as preventive dental cares in Korea, medical technician law confines duties of dental hygienists as closed narrative. The aim of this study was to investigate difference in perception of duties of dental hygienists in dental clinic between dentists and dental hygiene students. A total of 245 copies of questionnaires were distributed to dentists and students by post-mail. Only 42 dentists and 30 students in an area replied these questionnaires about the present and future duties of dental hygienists after providing written consent. Both groups agreed that intra and extra oral X-ray taking, education about oral health behavior, instruction after dental treatment, chair-side assisting, consulting for patients, scaling, initial impression taking, management of dental materials and equipment, sterilization of equipment, and receiving dental bills are duties of dental hygienists. However, they had different perceptions about various dental treatments as duties of dental hygienists even if they were under instructions of a dentist, including infiltration anesthesia, filling in cavity, intramuscular injection, FC change, canal irrigation, orthodontic treatment including separating, ligature bracket bonding and removing, setting crown and bridge, making individual, removing implant screw, and so on. These findings demonstrated that there were different perceptions about duties of dental hygienists between dentists and dental hygiene students, especially on dental treatment.

Adolescent delinquent behavior and the influence of friends: With specific focus on self-efficacy, parent-child conflict and parental control (친구가 청소년의 일탈행동에 미치는 영향: 자기효능감, 부모자녀 갈등 및 부모의 통제를 중심으로)

  • Young-Shin Park;Uichol Kim
    • Korean Journal of Culture and Social Issue
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    • v.16 no.3
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    • pp.385-422
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    • 2010
  • This study examines adolescent delinquent behavior and the influence of friends, focusing specifically on friends' delinquent behavior and the influence of self-efficacy, parent-child conflict and parental control. A total of 1,399 adolescents attending five different high schools (male=642, female=756, consisting of 915 student attending high school and 484 students attending vocational high school) completed a questionnaire developed by Ahn, Hwang, Kim and Park (1997) and Bandura's (1995a) self-efficacy scale. Results indicate that those students who attend high school had parents with higher education, socio-economic status and better studying environment at home, while students attending vocational high school had higher parent-child conflict. Students attending high school had higher self-efficacy scores, while students attending vocational high school had higher scores on delinquent behavior. The results of LISREL analyses revealed a similar pattern for high school and vocational high school students. Combined analysis indicate that friends' delinquent behavior, parent-child conflict and parental control had direct and positive effect on students' delinquency behavior. Self-efficacy had a direct and negative influence of delinquency behavior. Similar pattern was obtained for friends' delinquency behavior, in which self-efficacy had a direct and negative influence of their delinquency behavior and their parent-child conflict and parental control had direct and positive effect on their delinquency behavior. In summary, those students who had lower self-efficacy, higher parent-child conflict and parental control, and with friends who are more likely to engage in delinquent behavior, had higher scores on delinquent behavior. Also, those students who had friends with lower self-efficacy scores and with higher parent-child conflict and parental control are more likely to engage in delinquent behavior, which in turn influenced their delinquent behavior. Friends' delinquent behavior had the greatest influence on students' delinquent behavior indicating the role of friends in influencing delinquency among adolescents.

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