• Applied Chemistry for Engineering
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• v.26 no.1
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• pp.29-34
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• 2015

N,N'-Diferuloyl-putrescine (DFP) present in plants such as Sophora japonica has been reported to have skin depigmentative and antioxidative activities. In this study, DFP, usually presents in nature a very little amount and its derivative (DFP-D) were synthesized in a large quantity for the use as functional cosmetical materials. The antioxidative activities of synthesized DFP and DFP-D were evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, chemiluminescence assay, and cell protective effect induced by $^1O_2$, stress. DFP and DFP-D showed DPPH radical scavenging activities ($FSC_{50}$) at $61.25{\pm}2.25{\mu}M$ and $12.92{\pm}0.72{\mu}M$, respectively. ROS (reactive oxygen species) scavenging activities ($OSC_{50}$) in the $Fe^{3+}-EDTA/H_2O_2$ system of DFP and DFP-D were 2 times ($1.84{\pm}0.12{\mu}M$) and 13 times ($0.174{\pm}0.01{\mu}M$), respectively higher than that of L-ascorbic acid. $^1O_2$, one of ROS playing a key role in the skin photo-aging, induces cellular membrane damages. DFP-D ($50{\mu}M$) showed good cell protective effects (${\tau}_{50}=80.2min$) about 2 times more than that of (+)-${\alpha}$-tocopherol (${\tau}_{50}=43.6min$). These results suggest that the great antioxidative activities of DFP and DFP-D could be applied to cosmetic industries as functional cosmetic materials.

• Journal of Animal Science and Technology
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• v.46 no.3
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• pp.469-478
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• 2004

Pig slurry, urine and fermented liquid manure were evaluated measuring electrical conductivity (EC), dry matter (DM) contents and other components. Samples were collected during a period from February to April, 2001: slurry samples from 70 storage tanks, urine samples from 19 and 20 fermented liquid manure samples from 109 pig farms in Jeju. Samples were analyzed for pH, EC, DM, $NH_4$-N, K, P, Ca, Mg, Na, BOD, S-S, Cd, Cr, Cu, Fe, Mn, Ni, Pb, Zn and OM. Relationships between EC or DM and various mineral and $NH_4$-N contents were examined using linear regression. EC, DM, all mineral contents and $NH_4$-N were higher in slurry samples than in urine or fermented liquid manure. EC, $NH_4$-N, Ca, Mg and Na concentration was similar in urine and fermented liquid manure, but K and DM values in liquid manure were twice as much as in urine. P concentration was higher in urine than in liquid manure. $NH_4$-N and Na in slurry, $NH_4$-N in urine and $NH_4$-N, P and Ca in fermented liquid manure were highly correlated with EC, while $NH_4$-N, P, Ca and Mg in slurry, $NH_4$-N and Ca in urine, and K, P, Ca, Mg, and Na in fermented liquid manure with DM (P<0.05). BOO in slurry (22,520 mg/mL) was higher than that in urine (4,763) and fermented liquid manure (2,701). Results indicated that slurry is a better fertilizer source than urine or fermented liquid manure. However, slurry may pollute soil more than urine or fermented liquid manure when applied to land, although the levels are not above the permit.

• Journal of Food Hygiene and Safety
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• v.29 no.3
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• pp.181-188
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• 2014

The objective of this study was to analyze the microbiological hazards of Astragalus membranaceus Bunge on the post-harvest processing. Samples from processing equipments (cleaner, water, cart, table, tray and packaging machine), personal hygiene (hand) and harvested crops (before washing, after washing, after sorting, and after drying) were collected from four farms (A, B, C, and D) located in Chungchengbuk-do, Korea. The samples were analyzed for sanitary indication bacteria and pathogenic bacteria. First, total aerobic bacteria and coliform in processing facilities were detected at the levels of 0.93~4.86 and 0.33~2.28 log CFU/$100cm^2$ and/mL respectively. In particular, microbial contamination in hand (5.43~6.11 and 2.52~4.12 log CFU/Hand) showed higher than processing equipments. Among the pathogenic bacteria, Bacillus cereus was detected at the levels of 0.33~2.41 log CFU/$100cm^2$, 1.48~3.27 log CFU/Hand and 0.67~3.65 log CFU/g in equipments, hands, and plants and Staphylococcus aureus were detected in cleaner, table, hand and harvested crops (before washing and after sorting) by qualitative test. Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella spp. were not detected. These results indicated that personal hygiene and processing equipments should be managed to reduce the microbial contamination of A. membranaceus Bunge. Therefore, management system such as good agricultural practices (GAP) criteria is needed for hygienic agricultural products.

• Journal of Food Hygiene and Safety
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• v.28 no.3
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• pp.258-266
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• 2013

This study was conducted to determine the PSL and TL properties of foods irradiated with electron beam and gamma-ray. 5 kinds of food including cereal, pulse, fish powder, dried vegetable and tea were irradiated at 0 to 10 kGy by electron beam accelerator or $^{60}Co$ gamma-ray irradiator. The PSL analysis showed negative results for most of the non-irradiated samples. Non-irradiated shrimp powder showed intermediate result. Irradiated samples gave negative or intermediate or positive value which presented the limitation of PSL technique. In TL analysis, there were TL glow curves at around $300^{\circ}C$ with low intensity on non-irradiated samples. Maximum peak in the range of $150-250^{\circ}C$ was appeared on irradiated samples. TL ratio obtained by re-irradiation with 1 kGy was less than 0.1 on non-irradiated samples and higher than 0.1 on irradiated samples. Therefore, in PSL measurement, electron-beam irradiated samples could obtain more clear results. TL analysis showed obvious difference between non-irradiated and irradiated samples. But the identification was impossible for the sample of rice and lemon tea. Because of it's low contents of mineral.

• Journal of fish pathology
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• v.23 no.1
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• pp.69-83
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• 2010

This study was aimed to investigate the effects of injection of garlic, Allium sativum, extract and immersion in garlic juice on the nonspecific immunity and the resistance against the artificial infection of Streptococcus iniae and Edwardsiella tarda of the olive flounder, Paralichthys olivaceus. The nonspecific immune mechanisms were assessed in terms of lysozyme activity, nitroblue-tetrazolium (NBT) assay and superoxide dismutase (SOD) activity etc. Relative percent survival (RPS) was assessed by the challenge with S. iniae BS10 or E. tarda KE-1. Almost of the garlic extract injected groups showed the enhanced level of the tested nonspecific immune factors. In the challenge test with S. iniae and E. tarda, RPS of 5% garlic extract pre-injected group was much higher than that of any other tested groups, respectively. Almost of the garlic juice immersion tested groups exhibited strengthened nonspecific immune defence factors, lysozyme activity, the number of lymphocytes and neutrophils, NBT reduction and SOD activity in kidney. In the challenge with S. iniae and E. tarda, RPS in the 0.25 g/L of garlic juice immersed group was much higher than any other tested groups, respectively. The results suggest that the garlic extract and juice would be effective to enhance the nonspecific immunity and protective ability of olive flounder against fish disease such as S. iniae and E. tarda.

• Journal of Gifted/Talented Education
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• v.15 no.2
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• pp.59-76
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• 2005

We examined the relations between the score of the divergent thinking in mathematical (Mathematical Creative Problem Solving Ability Test; MCPSAT: Lee etc. 2003) and non-mathematical situations (Torrance Test of Creative Thinking Figural A; TTCT: adapted for Korea by Kim, 1999). Subjects in this study were 213 eighth grade students(129 males and 84 females). In the analysis of data, frequencies, percentiles, t-test and correlation analysis were used. The results of the study are summarized as follows; First, mathematically gifted students showed statistically significantly higher scores on the score of the divergent thinking in mathematical and non-mathematical situations than regular students. Second, female showed statistically significantly higher scores on the score of the divergent thinking in mathematical and non-mathematical situations than males. Third, there was statistically significant relationship between the score of the divergent thinking in mathematical and non-mathematical situations for middle students was r=.41 (p<.05) and regular students was r=.27 (p<.05). A test of statistical significance was conducted to test hypothesis. Fourth, the correlation between the score of the divergent thinking in mathematical and non-mathematical situations for mathematically gifted students was r=.11. There was no statistically significant relationship between the score of the divergent thinking in mathematical and non-mathematical situations for mathematically gifted students. These results reveal little correlation between the scores of the divergent thinking in mathematical and non-mathematical situations in both mathematically gifted students. Also but for the group of students of relatively mathematically gifted students it was found that the correlations between divergent thinking in mathematical and non-mathematical situations was near zero. This suggests that divergent thinking ability in mathematical situations may be a specific ability and not just a combination of divergent thinking ability in non-mathematical situations. But the limitations of this study as following: The sample size in this study was too few to generalize that there was a relation between the divergent thinking of mathematically gifted students in mathematical situation and non-mathematical situation.

• Korean Journal of Food Science and Technology
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• v.14 no.3
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• pp.250-256
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• 1982

A series of studies were conducted to find out the possibility of utilizing grape seed as resources of food fats and proteins, and the results of the studies are as follows: The grape seed contained 25.1%, of crude fat and 12.0% of crude protein. The lipid, fractions obtained by silicic acid column chromatography were mainly composed of about 95.5% neutral lipid, whereas compound lipid was only 4.5% level. Among the neutral lipid by thin layer chromatography, triglyceride was 91.89%, sterol ester, sterol, diglyceride and free fatty acid were 3.24%, 2.87%, 1.20% and 0.80%, respectively The predominant fatty acids of total and neutral lipids were linoleic acid $(69.72{\sim}71.72%)$ and oleic acid $18.09{\sim}19.46%)$, but those of glycolipid and phospolipid were linoleic acid $(31.49{\sim}38.18%)$, oleic acid $(20.20{\sim}35.27%)$ and palmitic acid $(26.80{\sim}39.98%)$. The major fatty acids of triglyceride separated from neutral lipid were oleic acid (43.08%), linoleic acid (38.42%) and palmitic acid (11.60%). The salt soluble protein of grape seed was highly dispersible in 0.02M sodium phosphate buffer containing about 1.0M $MgSO_4$, and the extractability of seed protein was 31%. Glutamic acid was the major amino acid in salt soluble protein, followed by arginine and aspartic acid. The electrophoretic analysis showed 3 bands in grape seed protein, and the collection rate of the main protein fraction purified by Sephadex G-100 and G-200 was 82%. Glutamic acid, aspartic acid and arginine were the major amino acids of the main grape seed protein. The molecular weight for the main protein of the grape seed was estimated to be 81,000.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.1
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• pp.53-62
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• 2006

Near infrared reflectance spectroscopy (NIRS) has been increasingly used as a rapid, accurate method of evaluating some chemical compositions in forages. Analysis of forage quality by NIRS usually involves dry ground samples. Costs might be reduced if samples could be analyzed without drying or grinding. The objective of this study was to investigate effect of sample preparations and spectral math treatments on prediction ability of chemical composition for corn silage by NIRS. A population of 112 corn silage representing a wide range in chemical parameters were used in this investigation. Samples of com silage were scanned at 2nm intervals over the wavelength range 400-2500nm and the optical data recorded as log l/Reflectance(log l/R) and scanned in overt-dried grinding(ODG), liquid nitrogen grinding(LNG) or intact fresh(IF) condition. Samples were analysed for neutral detergent fiber(NDF), acid detergent fiber(ADF), acid detergent lignin(ADL), crude protein(CP) and crude ash content were expressed on a dry-matter(DM) basis. The spectral data were regressed against a range of chemical parameters using modified partial least squares(MPLS) multivariate analysis in conjunction with four spectral math treatments to reduce the effect of extraneous noise. The optimum calibrations were selected on the basis of minimizing the standard error of cross validation(SECV). The results of this study show that NIRS predicted the chemical parameters with very high degree of accuracy(the correlation coefficient of cross validation$(R^2cv)$ range from $0.70{\sim}0.95$) in ODG. The optimum equations were selected on the basis of minimizing the standard error of prediction(SEP). The Optimum sample preparation methods and spectral math treatment were for ADF, the ODG method using 2,10,5 math treatment(SEP = 0.99, $R^2v=0.93$), and for CP, the ODG method using 1,4,4 math treatment(SEP = 0.29. $R^2v=0.91$).

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.4
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• pp.287-296
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• 2002

A field trial was conducted from May to November 2000 at four regions of Jeju province(Jeju, Seogwipo, Susan Seongsan and Keumak Hallim) to estimate adaptation, dry matter yield and nutrient value of kenafs(Everglades 41 and China Choung-pi 3) and a sorghum-sudangrass hybrid(Pioneer 947). Total dry matter(DM) yields of sorghum-sudangrass hybrid Pioneer 947, kenaf Everglades 41 and kenaf China Chong-pi 3 were 7,313, 4,653 and 5,238 kg/ha(P<0.05), respectively, kenaf China Chong-pi 3 being the highest. The region specific dry matter(DM) yields of Jeju, Seogwipo, Seongsan and Hallim area were 5,040, 5,663, 7,283 and 4,950kg/ha, respectively, showing that Seogwipo was the highest. Average DM yields of two kenaf varieties were 4,946, and 3,246 kg/ha for 1st time-cutting and 2nd time-cutting, respectively. The average dry matter(DM) yield of kenaf was significantly higher in the 1st time-cutting than the 2nd time. No significant difference in neutral detergent fiber(NDF) and acid detergent fiber(ADF) contents was found among three varieties. The average crude protein(CP) content of kenaf was higher than that of sorghum-sudangrass hybrid(P<0.05) and was higher in 2nd time-cutting than in 1st time-cutting(P<0.05). There were no significant difference in the heavy metal content of soil either between the sorghum hybrid and kenafs or between two kenaf varieties. In conclusion, the average dry matter(DM) yield of kenaf was lower than that of sorghum hybrid, Contrarily the crude protein(CP) content of kenafs was higher than that of the sorghum hybrid. The average dry matter(DM) yield of kenaf showed higher in 1st time-cutting than 2nd time-cutting, while the crude protein(CP) content of kenafs decreased with time.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.2
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• pp.152-158
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• 2015

This study was conducted from 2013 to 2014 in the middle region of Korea to compare the agronomic characteristics, forage production, and quality of kenaf, maize, and a sorghum ${\times}$ sudangrass hybrid. The three crops used in this study were kenaf "Hongma 300," maize "Kwangpyeongok," and the sorghum ${\times}$ sudangrass hybrid "Jumbo" (headless type). The sugar contents of the kenaf, maize, and sorghum ${\times}$ sudangrass hybrid were 5.4%, 4.4%, and 2.5%, respectively. The emergence rates and lodgings of the three crops were sound, as they were more than 80% and 1.5, respectively. The fresh yields of kenaf, maize, and the sorghum ${\times}$ sudangrass hybrid were 117,521 kg/ha, 73,336 kg/ha, and 133,334 kg/ha, respectively, and the dry matter (DM) yields were 28,598 kg/ha, 19,951 kg/ha and 20,083 kg/ha, respectively. The DM yield of kenaf was significantly the highest among the three crops (p<0.05). However, the total digestible nutrients (TDN) contents of the kenaf, corn, and sorghum ${\times}$ sudangrass hybrid were 43.3 %, 72.0 %, and 54.8 %, respectively, and the in vitro digestible dry matter (IVDDM) yields were 44.4%, 73.7%, and 59.6 %, respectively; therefore, the TDN yield of the corn was 13 % significantly higher than that of the kenaf (p<0.05). The TDN yields of the kenaf, corn, and sorghum ${\times}$ sudangrass hybrid were 12,472 kg/ha, 14,350 kg/ha, and 11,001 kg/ha, respectively. Among the three crops, the content of neutral detergent fiber (NDF) and acid detergent fiber (ADF) were highest in the kenaf. This study shows that the kenaf had a good forage productivity but a low forage quality.