• International Journal of Industrial Entomology and Biomaterials
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• v.17 no.2
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• pp.217-221
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• 2008

Dung beetle larvae at the $3^{rd}$ instar were injected with lipopolysaccaride and inducible proteins were examined within a pI level of 3-10 and a size level by proteomics, including 1-D SDS PAGE analysis and antibacterial assay. The immune infected larvae extracts provided seven protein bands in one-dimensional electrophoresis and its antibacterial activity also checked. Hemolymph protein from immune infected larvae of the dung beetle were separated by twodimensional gel electrophoresis and compared with those from native larvae. In 2-D gel electrophoresis, we detected 63 immune infected unique and 32 up-regulated proteins, and 36 proteins that were down-regulated or not present in treated gel. Ten protein spots from unique proteins and those presented as different level of abundance in infected and native larvae were specially expressed. These differentially expressed proteins were proposed to be involved in the defense mechanism against microorganism.

• International Journal of Industrial Entomology and Biomaterials
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• v.17 no.2
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• pp.223-228
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• 2008

A novel beetle antimicrobial protein from stimulated Copris tripartitus and the corresponding gene were isolated in parallel through differential display-PCR and expression in Escherichia coli. To find cDNA clones responsible for bacteria resistance, the suppression subtractive hybridization and GeneFishing differentially expressed genes system were employed in the dung beetle, Copris tripartitus immunized with lipopolysaccaride. One cDNA clone from eight subtracted clones was selected through dot blot analysis and confirmed by northern blot analysis. The 516-bp, selected cDNA clone was determined by 5' and 3' rapid amplication of cDNA ends and cloned into the GST fusion expression vector pGEX-4T-1 for expression of the protein. The expressed protein was predicted 14.7 kDa and inhibited the growth of gram-negative bacteria such as Escherichia coli and Pseudomonas aeruginosa. These results implied that the expressed protein is related to immune defense mechanism against microorganism.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.32 no.9
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• pp.72-79
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• 2004

A method for thruster fault diagnosis for launch vehicle upper stage was developed. In order to protect the launch vehicle against the occurrence of faults, it is necessary to detect and identify the fault, as well as to reconfigure the controller of the vehicle. Considering the upper stage launch vehicle using reaction control system, an analytical method was adopted in order to detect the fault occurred in thruster. The fault detection scheme can be applied to the system regardless of the form of thruster fault occurred - leakage or lock-out. Results from processor-in-the-loop simulation are provided to demonstrate the validity of this fault detection and isolation scheme for the upper stage launch vehicle.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2007.04a
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• pp.43-46
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• 2007

This study dealt with the influence of the replacement ratio of rapidly-chilled steel slag on fluidity and compressive strength of unsaturated polyester polymer concretes. The rapidly-chilled steel slag used in this study, a by-product which is produced by refining pig iron during the manufacture of steel, was controled by a air-jet method which rapidly cools substance melted at a high temperature. Experimental results show that fluidity and compressive strength of unsaturated polyester polymer concretes increase with increasing replacement ratio of rapidly-chilled steel slag. Use of rapidly-chilled steel slag was found to be effective for improving fluidity and compressive strength of rapidly-chilled steel slag.

• Korean Journal of Agricultural Science
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• v.40 no.3
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• pp.191-196
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• 2013

This study was conducted to investigate the effect of temperature during transportation and continuing shelf-life on fruit quality and the occurrence of physiological disorder to set up the appropriate exportation temperature condition in Asian pear (Pyrus pyrifolia Nakai) 'Hanareum'. In the experiment of simulated exportation headed for USA, the fruits transported at $1^{\circ}C$ showed less weight loss than those of $5^{\circ}C$. Market temperature appeared as a key factor for keeping freshness of exported pear fruits rather than transportation temperature. Quality factors such as high flesh firmness and low incidence of fruit rot and physiological disorders including core breakdown and pithiness were attained at the fruits maintained at $18^{\circ}C$. Approximately two times higher incidence of physiological disorders and of fruit decay rates were observed in the fruits distributed at $25^{\circ}C$ than the those of $18^{\circ}C$. Therefore, temperature management during marketing resulted as an important factor for maintaining fruits quality in the process of pear fruit exportation.

• Journal of the Korean Society of Combustion
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• v.14 no.4
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• pp.7-16
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• 2009

Porous metal plates (Metal fiber, muti-hole metal plate) using mainly in surface burner are known to have a corrosion and durability problem under high temperature condition. In this study, premixed flat flame with perforated ceramic tile of more durable cordierite material was examined with respect to combustion stability and emission. The flat premixed ceramic burner consists of perforated ceramic tile and various type of baffle plates to form stable surface flame. The results show that most stable flat flame is generated using baffle plate with open ratio of 0.193. In downward flat flame mode which is widely used in condensing boiler, CO is measured below 50ppm from equivalence ratio 0.755 to 0.765 and $NO_X$ is measured below 12ppm from equivalence ratio 0.75 to 0.79. It is also found that the range of blue flame in flame stability curve becomes wider with increasing heat capacity.

• Journal of Korean Medicine Rehabilitation
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• v.18 no.3
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• pp.157-171
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• 2008

Objectives : To report one case of semi-coma patient was treated by scalp acupuncture with electric stimulation. Methods : Patient (state of semi-coma) was treated by scalp acupuncture with electric stimulation, herbal medication and physical therapy. The improvement of patient was measured every other week by Glasgow Coma Scale(GCS), Modified Bathel Index(MBI), National Institutes of Health Stroke Scale(NIHSS). Results : We observed the patient decrease in NIHSS score and increase in GCS and MBI. Symptoms of patient such as semi-coma were improved following the treatment. Conclusions : It is suggested that oriental medical treatment; scalp acupuncture with electric stimulation, herbal medication and physical therapy were effective in semi-coma caused by traumatic brain injury.

• Journal of Korean Medicine Rehabilitation
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• v.18 no.3
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• pp.211-217
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• 2008

Objectives : The purpose of this study is to evaluate the clinical effect of acupuncture and electroacupuncture by meridian theory to a patient with writer's cramp. Methods : We treated this patient with only acupuncture and electroacupuncture by meridian theory. We evaluated the state of improvement by time to maintain right 2nd finger extended, Pain Disability Index(PDI). PDI was measured twice before treatment and after 19 weeks. Results and Conclusions : The subject was improved in estimation by time to maintain right 2nd finger extended, PDI. The time was from a few seconds to almost 90 minutes. PDI was from 21 to 17. According to above result, acupuncture and electroacupuncture by meridian theory were effective to improve symptoms of writer's cramp.

• Proceedings of the KSME Conference
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• 2008.11b
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• pp.2822-2827
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• 2008

Among the main components of PEM fuel cell, the functions of GDL are to transport reactants from the channel to the catalyst and remove reaction products from the catalyst and transport heat from the catalyst to the channels in the flow filed plate. Permeability of GDL is known to make it possible to enhance the gas transport through GDL, devoting to get better performance. In this paper, three dimensional numerical simulation of the fuel cell by the permeability of GDL is presented by using a FLUENT modified to include the electrochemical behavior. Results show that as permeability is higher than $10^{-12}m^2$, gradients of temperature distribution, oxygen molar concentration and current density distribution in MEA were decreased. Although heat generation was increased as high permeability, MEA's temperature was lower than the low permeability of GDL. This seems because that convection was higher affects in mass and heat transfer process than diffusion as permeability of GDL is increases.

• Proceedings of the KSME Conference
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• 2008.11b
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• pp.2204-2209
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• 2008

Flame pattern in burner used in steel industry that constitutes 30% of country energy consumption is generally characterized as long narrow flame pattern so that localized heating causes product quality worse and many burners are needed for proper heating. This paper deals with flat wide flame pattern which has advantage in terms of uniform heating using less number of burners. For that purpose, impinging jet system of fuel and oxidant was used for making flat wide flame. Results show that nozzle angle $75^{\circ}$ of impinging jet is found to be optimum configuration for making effective wide flame which has uniform radiation heat transfer and flame temperature is also most uniform along the flame width for that nozzle angle.