• Title/Summary/Keyword: Husbandry

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Do Government Subsidies Crowd In or Crowd Out R&D Investment? Evidence from China's Animal Husbandry Companies

  • XU, Jian;SIM, Jaewoo
    • Asian Journal of Business Environment
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    • v.10 no.4
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    • pp.5-13
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    • 2020
  • Purpose: The purpose of this paper is to empirically investigate the relationship between government subsidies and research and development (R&D) investment of animal husbandry companies in China. The moderating effects of firm size, debt ratio, and firm profitability on this relationship are also examined. Research design, data and methodology: The analysis is based on 14 animal husbandry companies listed on the Shanghai and Shenzhen stock exchanges over the period of 2012-2016. Data are obtained from the China Stock Market & Accounting Research (CSMAR) database and the RESSET database, and multiple regression analysis is utilized with the aid of Stata. Results: The empirical results show that government subsidies can promote R&D investment of animal husbandry companies in China. In addition, firm size, debt ratio, and firm profitability have positive moderating effects on the relationship between government subsidies and R&D investment. Conclusions: Based on the results, the paper concludes that government subsidies play an important role in the process of R&D of China's animal husbandry companies. This paper recommends that managers of animal husbandry companies should enhance the utilization efficiency of government subsidies and put great emphasis on R&D investment. The policymakers should implement more incentives to encourage animal husbandry companies to invest more in R&D.

Combined transcriptome and proteome analyses reveal differences in the longissimus dorsi muscle between Kazakh cattle and Xinjiang brown cattle

  • Yan, XiangMin;Wang, Jia;Li, Hongbo;Gao, Liang;Geng, Juan;Ma, Zhen;Liu, Jianming;Zhang, Jinshan;Xie, Penggui;Chen, Lei
    • Animal Bioscience
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    • v.34 no.9
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    • pp.1439-1450
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    • 2021
  • Objective: With the rapid development of proteomics sequencing and RNA sequencing technology, multi-omics analysis has become a current research hotspot. Our previous study indicated that Xinjiang brown cattle have better meat quality than Kazakh cattle. In this study, Xinjiang brown cattle and Kazakh cattle were used as the research objects. Methods: Proteome sequencing and RNA sequencing technology were used to analyze the proteome and transcriptome of the longissimus dorsi muscle of the two breeds of adult steers (n = 3). Results: In this project, 22,677 transcripts and 1,874 proteins were identified through quantitative analysis of the transcriptome and proteome. By comparing the identified transcriptome and proteome, we found that 1,737 genes were identified at both the transcriptome and proteome levels. The results of the study revealed 12 differentially expressed genes and proteins: troponin I1, crystallin alpha B, cysteine, and glycine rich protein 3, phosphotriesterase-related, myosin-binding protein H, glutathione s-transferase mu 3, myosin light chain 3, nidogen 2, dihydropyrimidinase like 2, glutamate-oxaloacetic transaminase 1, receptor accessory protein 5, and aspartoacylase. We performed functional enrichment of these differentially expressed genes and proteins. The Kyoto encyclopedia of genes and genomes results showed that these differentially expressed genes and proteins are enriched in the fatty acid degradation and histidine metabolism signaling pathways. We performed parallel reaction monitoring (PRM) verification of the differentially expressed proteins, and the PRM results were consistent with the sequencing results. Conclusion: Our study provided and identified the differentially expressed genes and proteins. In addition, identifying functional genes and proteins with important breeding value will provide genetic resources and technical support for the breeding and industrialization of new genetically modified beef cattle breeds.

The Use of Recommended Goat Husbandry Practices by Farmers in Southern Thailand

  • Pattamarakha, K.;Tanapannarachwong, J.;Saithanoo, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.6
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    • pp.587-592
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    • 1997
  • The extent to which farmers use the recommended husbandry practices by farmers raising goats in southern Thailand was investigated. Base-line data was collected by interviewing 297 farmers and the constraints to the use of recommended husbandry practices were examined for these farmers in the Chana district, Songkhla province of southern Thailand. The number of farmers using the various recommended husbandry practices was low. An extension program is needed to encourage the use of recommended goat husbandry practices in target areas of southern Thailand to provide a better understanding of the most effective goat husbandry practices.

The Genetic Diversity of Seven Pig Breeds in China, Estimated by Means of Microsatellites

  • Li, X.;Li, K.;Fan, B.;Gong, Y.;Zhao, S.;Peng, Z.;Liu, B.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.9
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    • pp.1193-1195
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    • 2000
  • The polymorphisms of six microsatellites were investigated in four indigenous pig breeds (Erhualian, Tongcheng, Qingping and Wannanhua) and three introduced breeds (Large White, Landrace and Duroc) in China, and the genetic variations within and among populations were analyzed. The results showed that genetic diversity of Chinese indigenous pig breeds is higher than that of the introduced pig breeds. The clustering of seven breeds is consistent with their geographical distribution approximately. Estimated time of breed divergence ranged from 653 to 1856 years.

Isolation, Serotyping and Nucleotide Sequence Analysis of Bovine Rotavirus Isolated from Korean Native Cattle (한우에서의 Rotavirus의 분리와 Serotype 결정 및 염기서열 분석)

  • Yu, Jae-Hyeun;Cha, Kwang-Jong;Kim, Eung-Ryool;Kim, You-Seong;Lee, Young-Kun;Song, Jin-Ook;Cho, Hong-Chan;Ju, Ji-Sun;Park, Bum-Suk;Yoo, Dea-Hwan;Kim, Se-Min;Ji, Byong-Ju;Lee, Joong-Bok
    • The Journal of Korean Society of Virology
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    • v.30 no.3
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    • pp.189-202
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    • 2000
  • This study was conducted to see what types of bovine rotaviruses were isolated at Jedong farm in Jeju province and Seohwa farm in Chungnum province. The results were as follows. 1. Rotavirus was positively detected in 18 out of 39 fecal samples from calves with diarrhea in Jeju province and in 13 out of 18 fecal samples from calves with diarrhea in Chungnam province. 2. The electropherotype pattern of dsRNA for 31 viruses was shown to be 4 : 2 : 3 : 2 type like traditional group A and the imigration pattern of dsRNA was the long type like NCDV (G6), JBR (G6), B223 (G10) and KK3 (G10). 3. The serotypes of the 18 viruses of Jedong and 9 viruses of Seowha were shown to be group A, subgroup I, G6, and P1 by ELISA and PCR analyses. The serotypes of S-2, S-6, S-9 and S-12 viruses of Seowha were shown to be group A, subgroup I, G10, but was not shown to be P type. 4. The partial nucleotide sequence of VP4 of S-8 was 97% homology with that of BRV 033. VP4 of J-10 showed 96% homology with that of BRV 033 in nucleotide sequence.

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Genome-wide association study for frozen-thawed sperm motility in stallions across various horse breeds

  • Nikitkina, Elena V.;Dementieva, Natalia V.;Shcherbakov, Yuri S.;Atroshchenko, Mikhail M.;Kudinov, Andrei A.;Samoylov, Oleg I.;Pozovnikova, Marina V.;Dysin, Artem P.;Krutikova, Anna A.;Musidray, Artem A.;Mitrofanova, Olga V.;Plemyashov, Kirill V.;Griffin, Darren K.;Romanov, Michael N.
    • Animal Bioscience
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    • v.35 no.12
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    • pp.1827-1838
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    • 2022
  • Objective: The semen quality of stallions including sperm motility is an important target of selection as it has a high level of individual variability. However, effects of the molecular architecture of the genome on the mechanisms of sperm formation and their preservation after thawing have been poorly investigated. Here, we conducted a genome-wide association study (GWAS) for the sperm motility of cryopreserved semen in stallions of various breeds. Methods: Semen samples were collected from the stallions of 23 horse breeds. The following semen characteristics were examined: progressive motility (PM), progressive motility after freezing (FPM), and the difference between PM and FPM. The respective DNA samples from these stallions were genotyped using Axiom Equine Genotyping Array. Results: We performed a GWAS search for single nucleotide polymorphism (SNP) markers and potential genes related to motility properties of frozen-thawed semen in the stallions of various breeds. As a result of the GWAS analysis, two SNP markers, rs1141327473 and rs1149048772, were identified that were associated with preservation of the frozen-thawed stallion sperm motility, the relevant putative candidate genes being NME/NM23 family member 8 (NME8), olfactory receptor family 2 subfamily AP member 1 (OR2AP1), and olfactory receptor family 6 subfamily C member 4 (OR6C4). Potential implications of effects of these genes on sperm motility are herein discussed. Conclusion: The GWAS results enabled us to localize novel SNPs and candidate genes for sperm motility in stallions. Implications of the study for horse breeding and genetics are a better understanding of genomic regions and candidate genes underlying stallion sperm quality, and improvement in horse reproduction and breeding techniques. The identified markers and genes for sperm cryotolerance and the respective genomic regions are promising candidates for further studying the biological processes in the formation and function of the stallion reproductive system.