• KSBB Journal
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• 제31권4호
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• pp.193-199
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• 2016

Global warming caused from the heavy consumption of fossil fuel is one of the biggest problems to be solved. Biofuel has been gained more attention as an alternative to reduce the consumption of fossil fuel. Recently, butanol produced from the genus Clostridium has been considered as one of the promising alternatives for gasoline, fossil based fuel. Nevertheless, the lack of the genome-engineering tools for the genus Clostridium is the major hurdle for the economic production of butanol. More recently, genome engineering tools have been developed for metabolic engineering of butanol-producing Clostridium species, which includes genome scale network model and genome editing tools on the basis of mobile group II introns and CRISPR/Cas system. In this study, the genome engineering tools for butanol-producing Clostridium species have been reviewed with a brief future perspective.

• Journal of Pharmaceutical Investigation
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• 제41권6호
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• pp.381-386
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• 2011

Multi-drug resistance (MDR) has been known as a major hurdle in cancer chemotherapy. One of the most clinically significant causes of MDR was the efflux of anticancer agents mediated by p-glycoprotein (p-gp) over-expressed in MDR cancer cells. To overcome MDR, there have been several strategies such as co-administration with p-gp inhibitors and encapsulation of anticancer drugs into drug delivery systems. In the present study, curcumin was evaluated for its potential as p-gp inhibitor and MDR reversal activity when combined with paclitaxel incorporated into lipid nanoparticles (PTX/LN). Western blot assay showed curcumin did not modulate the level of p-gp expression in MCF-7/ADR which is a MDR variant of human breast cancer cell line, MCF-7, and over-expresses p-gp. However, curcumin inhibited p-gp-mediated efflux of calcein in a dose-dependent manner even though it showed lower activity compared to verapamil, a well-known p-gp inhibitor. Incorporation of paclitaxel into lipid nanoparticles partially recovered the anticancer activity of paclitaxel in MCF-7/ADR. The combined use of curcumin and PTX/LN exhibited further full reversal of MDR, suggesting susceptibility of PTX/LN to the efflux system. In conclusion, combined approach of using p-gp inhibitors and incorporation of the anticancer agents into nano-delivery systems would be an efficient strategy to overcome MDR.

• 대한한의학방제학회지
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• 제14권1호
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• pp.201-203
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• 2006

There are people. however, who do not understand what science means, especially among those practicing Oriental Medicine. They associate science with machines and consider that science is something not applicable to Oriental Medicine because Medicine is for humans not for machines. Science is a system of knowledge of universal facts and laws obtained based on objectivity, which under the same conditions gives the same results no matter when, where and how it is done and no matter who does it. To achieve development of that level, knowledge and technology from all areas should be considered and, if found useful, should be accepted. The immediate hurdle is to make Bang-Jae Science understood. So far Bang-Jae Discipline as understood by the practitioners of Oriental Medicine is nothing more than selecting prescriptions inherited from the predecessors that match given symptoms. To make Bang-Jae Discipline a science. i.e. Bang-Jae Science, we should not only study the traditional literature to gather data, experiment and verify with them one after another. make them objective and carry our scientific systemization. It takes fresh and creative trials but it will surely contribute to the future medical science.

• Journal of Electrical Engineering and Technology
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• 제6권5호
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• pp.605-612
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• 2011

As the power grids are integrated into one big umbrella called a "smart grid," communication protocol plays a key role in successful operations. The successful deployment of smart grid interoperability is a major hurdle that must be overcome. The micro grid, a small power system that distributes energy resource, is operated in diverse regions. Different vendors use different communication protocols in the operation of the micro grid. Recently, the IEC 61850 has been legislated to solve the interoperability problems in power utility automation. The present paper presents a micro grid system based on the IEC 61850 protocol. It consists of a micro grid monitoring system, a protocol converter that transforms serial data to IEC 61850 data, and distributed energy resource controllers for diverse DER nodes. A developed communication gateway can be deployed for DER controllers with serial links to exchange data with IEC 61850-based devices. The gateway can be extended to IEC 61850-based distribution automation systems, substation automation systems, or SCADA.

• 기술혁신학회지
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• 제21권4호
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• pp.1508-1535
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• 2018

기업들에 있어서 고용은 혁신활동의 결과를 바탕으로 하는 연속된 의사결정과정이다. 따라서 기술혁신을 통한 고용성과를 살펴봄에 있어서 이 같은 관계성을 명시적으로 분석에 고려할 필요가 있다. 이러한 문제의식을 바탕으로 본 연구에서는 중소기업의 기술혁신과 고용효과의 관계를 선행 연구와 같이 단일방정식을 추정하는 순위로짓(ordered logit) 모형으로 분석할 뿐 아니라, 연립방정식을 추정하는 외견무관회귀(SUR) 모형을 활용하여 실제 동시적으로 수행되어지는 기술경영 과정의 상관성을 반영하여 기업의 혁신활동과 기술혁신 성과, 보상제도 그리고 고용 창출의 구조적 관계를 분석하고자 하였다. 분석 결과, 보상제도의 시행은 기술개발 및 기술사업화 성과를 높이고, 이것은 다시 기업의 고용을 촉진하는 구조적 관계를 확인할 수 있었다. 특히 고용성과에 있어 기술개발 뿐 아니라 사업화 성과가 일종의 허들로 작용한다는 점에서 기술혁신을 프로세스 관점에서 관리할 때 기업의 고용성과는 높아진다고 볼 수 있겠다.

• 상하수도학회지
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• 제32권5호
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• pp.399-409
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• 2018

Prussian blue is known as a superior material for selective adsorption of radioactive cesium ions; however, the separation of Prussian blue from aqueous suspension, due to particle size of around several tens of nanometers, is a hurdle that must be overcome. Therefore, this study aims to develop granule type adsorbent material containing Prussian blue in order to selectively adsorb and remove radioactive cesium in water. The surface of granular activated carbon was grafted using a covalent organic polymer (COP-19) in order to enhance Prussian blue immobilization. To maximize the degree of immobilization and minimize subsequent detachment of Prussian blue, several immobilization pathways were evaluated. As a result, the highest cesium adsorption performance was achieved when Prussian blue was synthesized in-situ without solid-liquid separation step during synthesis. The sample obtained under optimal conditions was further analyzed by scanning electron microscope-energy dispersive spectrometry, and it was confirmed that Prussian blue, which is about 9.7% of the total weight, was fixed on the surface of the activated carbon; this level of fixing represented a two-fold improvement compared to before COP-19 modification. In addition, an elution test was carried out to evaluate the stability of Prussian blue. Leaching of Prussian blue and cesium decreased by 1/2 and 1/3, respectively, compared to those levels before modification, showing increased stability due to COP-19 grafting. The Prussian blue based adsorbent material developed in this study is expected to be useful as a decontamination material to mitigate the release of radioactive materials.

• 한국산학기술학회논문지
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• 제19권9호
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• pp.427-434
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• 2018

Pavitt(1984)과 Malerbar(2002)의 지적과 같이 혁신은 산업의 특성에 따라 다른 면모를 갖는다. 이 같은 관점에서 기계산업의 혁신을 다룬 기존 연구들은 주로 전체 제조업과 비교한 산업간 차이를 규명하는 데 초점을 두었다. 기계산업과 같은 기술 숙련집약적 산업에서의 혁신은 특히 다른 산업의 혁신과 상이한 특성과 메커니즘을 가질 것으로 예상되는 반면, 논의는 부족한 실정이다. 이에 본 연구에서는 기계산업의 제품혁신이 어떠한 요인들에 의해 결정되는 지를 다룸에 있어 제품혁신의 성과를 양적 측면과 질적 측면으로 구분해 접근하였고, 특히 Heckman 2단계 모형과 허들음이항 모형을 적용해 기업의 혁신활동에 수반되는 단계적 선택과정을 명시적으로 분석에 반영하였다. 분석 결과, 기계산업의 제품혁신에 있어 제품의 품질 및 성능향상 및 특허에 영향을 미치는 요인은 서로 상이하였으며, 문지방효과와 혁신의 강도 역시 구분되는 것으로 나타났다. 이는 결과적으로 기계산업은 연구개발 역량을 일정 수준 이상 축적하고 외부의 혁신을 효과적으로 조직 내 체화하고, 조직 구성원들에 대한 동기부여가 성과 향상에 중요한 역할을 수행한다고 하겠다.

• Genomics & Informatics
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• 제10권1호
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• pp.33-39
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• 2012

High-throughput genomic technologies (HGTs), including next-generation DNA sequencing (NGS), microarray, and serial analysis of gene expression (SAGE), have become effective experimental tools for cancer genomics to identify cancer-associated somatic genomic alterations and genes. The main hurdle in cancer genomics is to identify the real causative mutations or genes out of many candidates from an HGT-based cancer genomic analysis. One useful approach is to refer to known cancer genes and associated information. The list of known cancer genes can be used to determine candidates of cancer driver mutations, while cancer gene-related information, including gene expression, protein-protein interaction, and pathways, can be useful for scoring novel candidates. Some cancer gene or mutation databases exist for this purpose, but few specialized tools exist for an automated analysis of a long gene list from an HGT-based cancer genomic analysis. This report presents a new web-accessible bioinformatic tool, called CaGe, a cancer genome annotation system for the assessment of candidates of cancer genes from HGT-based cancer genomics. The tool provides users with information on cancer-related genes, mutations, pathways, and associated annotations through annotation and browsing functions. With this tool, researchers can classify their candidate genes from cancer genome studies into either previously reported or novel categories of cancer genes and gain insight into underlying carcinogenic mechanisms through a pathway analysis. We show the usefulness of CaGe by assessing its performance in annotating somatic mutations from a published small cell lung cancer study.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2000년도 춘계 산학협동 심포지움 Proceedings
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• pp.23-36
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• 2000

Hurdle technique was used to remove cholesterol efficiently from liquid egg yolk. The quality of the low cholesterol egg products from the process were evaluated. From the 75 % cholesterol reduced egg yolk through $\beta$-cyclodextrin treatment. 2 times weight of soy bean oil was added to the egg yolk and homogenized followed by centrifuged to be maximized to remove cholesterol. When the pH of the yolk was adjusted to 9, 92 % of cholesterol was removed while 95.4 % of cholesterol was removed when 3 times weight of soy bean oil was added to the egg yolk. As the results of application of supercritical carbon dioxide extraction to the 75 % cholesterol reduced egg yolk through ${\beta}$-cyclodextrin treatment, 92.5 % of the cholesterol was removed from the egg yolk at $35^{\circ}C$, 4,500 psi, for 4 hours under co-solvent. The quality characteristics of the produced low cholesterol egg products were analysed. The cholesterol reduced egg yolk produced from ${\beta}$-cyclodextrin and soy bean oil treatment showed the lower emulsion capacity compared with control. The fatty acid composition of the cholesterol reduced egg yolk produced from ${\bet}a$-cyclodextrin and soy bean oil treatment showed increased C18:2 and C18:3 compared with control while decreased C16:1 and C18: 1 compared with control. The saponification method with extracting solvent of hexane showed that cholesterol concentration was 28.1 %. The quantity of hydrolysis solution(95 % ethanol : 33 % KOH = 94 : 6) was varied from 40 to 80 times of sample weights and the cholesterol concentration of 35.7 % was the highest result in the 60 times(v/w) hydrolysis solution. Cholesterol concentration of 35.7 % was recovered at the first trial with saponification method. but it could be improved up to 95.7 % after 4 times repetitive purification.

• Journal of Information Science Theory and Practice
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• 제7권1호
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• pp.29-38
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• 2019

Information retrieval is the challenge of the Web 2.0 world. The experiment of knowledge organisation in the context of abundant information available from various sources proves a major hurdle in obtaining information retrieval with greater precision and recall. The fast-changing landscape of information organisation through social networking sites at a personal level creates a world of opportunities for data scientists and also library professionals to assimilate the social data with expert created data. Thus, folksonomies or social tags play a vital role in information organisation and retrieval. The comparison of these user-created tags with expert-created index terms, author keywords and title words, will throw light on the differentiation between these sets of data. Such comparative studies show revelation of a new set of terms to enhance subject access and reflect the extent of similarity between user-generated tags and other set of terms. The CiteULike tags extracted from 5,150 scholarly journal articles in marine science were compared with corresponding Aquatic Science and Fisheries Abstracts descriptors, author keywords, and title terms. The Jaccard similarity coefficient method was employed to compare the social tags with the above mentioned wordsets, and results proved the presence of user-generated keywords in Aquatic Science and Fisheries Abstracts descriptors, author keywords, and title words. While using information retrieval techniques like stemmer and lemmatization, the results were found to enhance keywords to subject access.