• The korean journal of orthodontics
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• v.28 no.4 s.69
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• pp.627-640
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• 1998

[$TGF-{\beta}$] is a polypeptide with multiple physiological functions in regulation of cell-to-cell interaction and in growth and development. The active form of vitmain $D_3$, 1,25-dihydroxycholecalciferol $[1,25-(OH)_2D_3]$, is one of the most potent stimulators of osteoclastic acitivity. The purpose of this study was to evaluate the effect of Vitamin $D_3$ and/or $TGF-{\beta}$ on the periodontal ligament(PDL) cells. Human PDL cells were prepared from the first premolars extracted for the orthodontic treatment and were incubated in the environment of , $37^{\circ},\;5\%\;CO_2\;and\;95\%$ humidity. 10, 50 or 100ng/m1 of $1,25-(OH)_2D_3$ and 0.1, 1, 5 or 10ng/ml of $TGF-{\beta}$ were administered to the culture wells, separately or in combination. And the viability of PDL cells was evaluated by MTT assay The obtained results were as follows. 1. The viability of PDL cells in 10ng/ml of vitamin $D_3$ was not significantly differenent from that of the control group at 1, 2 and 3-day of cultivation, but it was significantly increased in 50ng/ml of Vitamin $D_3$ at 3-day and in 100ng/m1 of Vitamin $D_3$ at 2 and 3-day. 2. The viability of PDL cells in 0.1ng/ml of $TGF-{\beta}$ was not significantly differenent from that of the control group at 1, 2 and 3-day of cultivation, but it was significantly increased in 1 and 5ng/ml of $TGF-{\beta}$ at 3-day of cultivation, and in 10ng/ml of $TGF-{\beta}$ at 2 and 3-day of cultivation. 3. In case of admixture of 1ng/ml of $TGF-{\beta}$ and the various concentrations of vitamin $D_3$, the viability of PDL cells was significantly increased in the admixture of 100ng/ml of vitamin $D_3$ at 3-day of cultivation 4. In case of admixture of 5ng/ml of $TGF-{\beta}$ and the various concentrations of vitamin $D_3$, the viability of PDL cells began to be increased from 2-day of cultivation in the admixture of 10 50 and 100ng/ml of vitamin $D_3$, but it was not maintained at 3-day in the admixture of 10ng/m of vitamin $D_3$. 5. In case of admixture of 10ng/ml of $TGF-{\beta}$ and the various concentrations of vitamin $D_3$ the viability of PDL cells was significantly increased in the admixture of 50ng/ml of vitamin $D_3$ at 2 and 3-day of cultivation, and in the admixture of 100ng/ml at 1, 2 and 3-day.

• Asian Journal of Turfgrass Science
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• v.18 no.2
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• pp.63-70
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• 2004

This study was conducted to figure out the effect of 'Methlyen Urea(MU)', slow released fertilizer and 'T-Vigor', microbial fertilizer as environment fertilizer on growth of 'Crenshaw' creeping bentgrass for environmental management in golf course. This study was conducted at No. 3, 4, 5 Valley Courses of Rexfield Country Club from April to July in 2004. MU, T-Vigor, sterilized T-Vigor were applied five times with 5g and 7.5ml per square meter, respectively. Polt size was 1 square meter and there were three replications with Completely Randomize Design. Collecting data were turf density$(No.\;of\;shoot\;/cm^2)$, chlorophyll $amount(\%)$, root length(cm), dry weight of clipping(g), and dry weight of root(g). The results are as follows; All of turf density, chlorophyll amount and dry weight(g) of MU and T-Vigor were better than control and sterilized T-Vigor. Especially root length of MU and T-Vigor was superior to control and sterilized T-Vigor, even if temperature and humidity was high. In conclusion, MU and T-Vigor might be used as slow release fertilizer for environmental green management in golf course.

• Journal of Bio-Environment Control
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• v.16 no.4
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• pp.314-321
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• 2007

This study was conducted to select leaf vegetables suitable for cultivation in apartment verandas and simple and easy fertigation method for home gardening. In order to develop the convenient fertigation method, hydroponics, wick irrigation, and overhead irrigation methods were compared. For the wick irrigation, two types of nutrient sources were used; one was slow release fertilizers mixed with medium and the other one was nutrient solution filled in container located under pots. The growth of leafy lettuce, leaf mustard, and leaf beet was better in both of the wick irrigation methods rather than in overhead irrigation and hydroponics. The wick irrigation method is very easy, so that it is expected to bring a good result from the cultivating and managing point of view, if it brings with commercialized system along with slow release fertilizer. As a result of investigation of environment such as temperature, relative humidity, and irradiance level in apartment verandas in autumn the highest irradiance level during a day was just 48% and 35% in verandas facing south and feeing southeast, respectively, comparing to that in greenhouse. The light environment was investigated as a limiting factor for vegetable growing in verandas. Therefore, to select the vegetables showing good growth under low irradiance environment, nine leaf vegetables such as romaine lettuce, lent lettuce, head lettuce, endive, pak-choi, leaf mustard, garland chrysanthemum, leaf beet, and Chinese chive were grown under 0%, 50%, 70%, 90% shading. Among them, Chinese chive showed the best growth under low irradiance levels. Endive showed line growth reduction according to shading degree, however, even under 90% shading condition, it showed good growth. And then leafy lettuce, garland chrysanthemum, and pak-choi followed. Therefore, these results will be of help in selecting vegetables for veranda gardening with different light levels.

• Journal of Bio-Environment Control
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• v.26 no.4
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• pp.324-332
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• 2017

The comparative experiments were conducted for single span greenhouses where cucumbers were cultivated to analyze the effect of heating between a carbon fiber electric heating element heater and an oil hot air heater in terms of the inside climate, energy consumption and plant growth. In order to analyze the effect of heating capacity, 6, 9, and 16 kW of electric powers were supplied to the electric heating element for same setting temperature of 15?. As a result, as the heating capacity increased, the number of ON-OFF cycles of the electric heating element and the temperature inside the greenhouse increased proportionally. In the comparison of two heaters, it was shown that the temperature and relative humidity distributions of the electric heating element installed greenhouse was much uniform than those of the oil hot air heater installed greenhouse. The heating energy consumptions during the heating period of 79 days were 867L for the oil hot air heater and 8,959 kWh for the electric heating element heater, and the heating costs were 607 and 403 thousand won respectively. In the electric heating element installed greenhouse, the cucumber growth was slightly better and the yield was 4.3% higher than those of the oil hot air heater installed greenhouse, but there were no statically significant difference in the cucumber growth and yield between greenhouses.

• Journal of Bio-Environment Control
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• v.26 no.4
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• pp.386-392
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• 2017

This study was conducted to examine the growth and phytochemical contents of spinach (Spinacia Oleracea L. 'Sushiro') as affected by different fluorescent lamps in a closed-type plant production system. Seeds were sown in a 128-cell plug tray filled in rockwool. The seedlings were transplanted into a DFT (deep floating technique) system with recycling nutrient solution (EC $1.5dS{\cdot}m^{-1}$ and pH 6.5) in a closed-type plant production system. The seedlings were grown under 3 types of fluorescent lamp, #S (NBFHF 32S8EX-D, CH LIGHTING Co. Ltd., China), #O (FHF32SSEX-D, Osram Co. Ltd., Germany), and #P (FLR32SS EX-D, Philips Co. Ltd., The Netherlands) at $150{\mu}mol{\cdot}m-2{\cdot}s^{-1}\;PPFD$ with a photoperiod of 14/10 (light/dark) hours. Plants were cultured under condition of $25{\pm}1^{\circ}C$ temperature and $60{\pm}10%$ relative humidity after transplanting. Thirty plants per each treatment were cultivated for $6^{th}$ week after transplanting. And growth and phytochemical contents were measured at $3^{rd}$ and $6^{th}$ week. At the $3^{rd}$ week after transplanting, the parameter values of plant height and leaf width were higher in the #O than the others. However, fresh and dry weights of root were the greatest in the #P. In addition, total phenolic concentration was the greatest in the #P. At $6^{th}$ week after transplanting, the #O had the greatest growth of spinach in the plant height and fresh and dry weights of shoot. The total phenolic contents significantly increased in the #O and showed significantly difference. However, there was no significant difference all treatments in antioxidant activity. Therefore, these results suggest that the #O was suitable for the growth and phytochemical accumulation of spinach in a closed-type plant production system.

• Journal of Nutrition and Health
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• v.3 no.3
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• pp.129-135
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• 1970

From the previous studies, F-P-4 formula was found to be comparable to full fat dry milk in its nutritive value and feeding performance. However, an attempt was made in order to make sure whether or not any possibility might exist, by which further improvement of nutritive quality and simultaneous reduction of product costs may be achieved. Using F-P-4 as a control, modifications were made in new formulas, F-P-5, F-P-6 and F-P-7 by reducing FPC, eliminating yeast from the mixture, and by enriching with methionine as needed. In particular, F-P-7 is completely free of FPC, hydrogenated oil and yeast. Yet, levels of total protein and fat were kept equal to those of F-P-4 in all formulas. An animal feeding test for all formulas using 10 female rats per group for 8 weeks and an infant feeding trial for F-P-5 and F-P-6 with 5 of each female infants under age of one for one month were conducted along with F-P-4 as a control. Almost the same results were obtained with F-P-4, 5 and 6, but F-P-7 showed the lowest body weight gain. FER of F-P-5 and 6 was 0.20 as was with F-P-4, while that of F-P-7 was 0.16. Acceptability to infants was excellent; growth, appearance and biochemical data were normal. As an example F-P-4 packed in 0.04mm polyethylene bags was used for storage study at $25^{\circ}C$ and relative humidity of $65{\sim}85%$ for 8 months. Although viable bacterial counts and vitamin C contents were reduced, peroxide and TBA values were increased gradually during such storage. Since there are also significant changes in color and organoleptic quality, the expected shelf life under the given conditions is considered to be about 2 months and thus further works are needed both on the product and packaging in order to improve the storage stability. Either elimination of yeast form F-P-4, that is F-P-5, or partial replacement of FPC with methionine, that is F-P-6 may well reduce material costs about 10%. Considering blending process of ingredients, F-P-5 is thus found to be the best formula developed. While F-P-7 free of FPC is inferior in its nutritive quality than that of others, but significantly superior than of rice. Furthermore, the material cost of the product can be reduced about 20% from that of F-P-4. And thus this vegetable blend is considered to be useful as a low cost supplementary food mixture for growing children.

• Journal of Bio-Environment Control
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• v.21 no.2
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• pp.88-94
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• 2012

This study was carried out in order to investigate the effect of temperature of treatment and storage on the longevity of 'Unicorn' tomatoes of light red maturity stage during commercial handling conditions encountered while exporting over long distances. Tomato stored at $5^{\circ}C$ and $11^{\circ}C$ temperature with 85% relative humidity by pre-treating handling temperature that was using from field to before shipment as a winter temperature $5^{\circ}C$, spring temperature $11^{\circ}C$ and summer temperature $25^{\circ}C$ for 3 days. On the final storage day, $25^{\circ}C/11^{\circ}C$ (treated/stored) tomatoes showed the highest respiration and ethylene production rate; whereas the lowest respiration and ethylene production rate was found for $5^{\circ}C/5^{\circ}C$ treated and stored tomatoes. Tomatoes treated and stored at $5^{\circ}C/5^{\circ}C$ showed higher marketability, without evidence of fungal rot, decay or spots for 23 days. The fresh weight loss under all treatment conditions increased gradually during $5^{\circ}C$ and $11^{\circ}C$ storage temperatures. The higher firmness and soluble solids were determined from $5^{\circ}C/5^{\circ}C$ and $5^{\circ}C/11^{\circ}C$ treated and stored tomatoes repectively, than from others tomatoes on the final day of storage. In addition, $5^{\circ}C/5^{\circ}C$ tomatoes showed higher vitamin C contents than tomatoes stored at other temperatures, on the final day of storage. As the ripening and storage period progressed, the titratable acidity increased, but declined (P < 0.05) thereafter, due to over ripe tomatoes under all treatment conditions. These results show that $5^{\circ}C/5^{\circ}C$ treated and stored light red maturity stages of 'Unicorn' tomatoes are optimum to export because they show the highest storability and marketability. Moreover, the marketability of light red maturity stage of 'Unicorn' tomato maintained for 2 weeks in $25^{\circ}C/11^{\circ}C$ treated and stored temperature that might be the export temperature from Korea to Japan in summer season. This research result could be useful in helping tomato growers and exporters to get optimum market value by satisfying the buyer and consumer with a fresher product.

• Journal of Bio-Environment Control
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• v.14 no.4
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• pp.233-238
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• 2005

Growth of Campanula punctata 'Rubriflora' plantlets, as affected by three levels of photosynthetic photon flux (PPF), 70, 110, and $220{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, two levels of $CO_2$ concentration, 500 and $1,500{\mu}mol{\cdot}m^{-1}$, and two levels of number of air exchanges per hour (NAEH), 0.1 and $2.8 h^{-l}$, was studied. Explants were obtained from photomixotrophically-micropropagated plantlets. Four explants were planted in each $3.7{\times}10^{-4}m^3$ polycarbonate box containing MS basal medium and no added sucrose. Explants were cultured under cool-white fluorescent lamps for $16h{\cdot}d^{-1},\;at\;25\pm1^{\circ}C$ temperature, and $70\~80\%$ relative humidity In treatments of $2.8h^{-1}$ NAEH, a 10mm round hole made on the vessel cap was sealed with a microporous filter. For higher $CO_2$ concentrations in the culture room, $CO_2$ gas was provided from a tank of liquefied $CO_2$. Fresh and dry weights, length of the longest root, and number of leaves significantly increased with increasing PPF and especially $CO_2$ concentration. Length of the longest root, number of leaves, fresh and dry weights, and chlorophyll concentration were enhanced with increased NAEH. However, leaf area was the smallest in the $220{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}\;PPF\;2.8h^{-1}$ NAEH and especially, $1,500{\mu}mol{\cdot}mol^{-1}\;CO_2$ concentration treatment. Treatment effect became more produced with time. Overall, treatment with $220{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}\;PPF\;and\;1,500{\mu}mol{\cdot}mol^{-1}\;CO_2$ gave the most vigorous growth.

• Journal of Bio-Environment Control
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• v.25 no.3
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• pp.162-167
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• 2016

This study was carried out to investigate proper stem cutting position and air temperature to regenerate roots from cuttings of bitter gourd. 'NS454' (NS) and 'Dragon' (DR) cultivars were tested and the cutting position was cut at the 3rd node in the stem (treatment I) and cut at the central part (stem segment) between 3rd leaf and 4th leaf in the stem (treatment II). The air temperature was maintained at 18, 23, 28 and $35^{\circ}C$ in the growth chambers, respectively. The photosynthetic photon flux at the ground of a chamber was maintained in approximately $150-200{\mu}mol\;m^{-2}s^{-1}$ during the 16-hour photoperiod. The relative humidity in the chambers was maintained over 85%. After 10 days of cuttings, regardless of cutting position, DR and NS cultivars showed 100% the survival rate in the $18^{\circ}C$ or $23^{\circ}C$ treatments, however, most of plants $18^{\circ}C$ treatment had not rooting. In the $28^{\circ}C$ treatment, regardless of cutting position, DR and NS cultivars showed 100% of the rooting rate. In the $23^{\circ}C$ treatment, for DR cultivar, the node cutting showed 90% of rooting rate and the stem segment cutting showed 40% rooting rate. For NS cultivar, the node cutting showed 50% of rooting rate and the stem segment cutting showed 40% rooting rate. DR and NS cultivars have high rooting rate, while the rooting rate remarkably decreased in the $35^{\circ}C$ treatment. For DR cultivar, the stem segment cutting showed 5.3 of the highest rooting number and the node cutting showed 2.7 rooting number in the $28^{\circ}C$ treatment. NS cultivar was not significantly different the rooting regardless of cutting position. The results suggested that cutting at the stem segment cutting of bitter gourd is an effective cutting method for increasing survival rate and the air temperature should be maintained at $28^{\circ}C$.

• Journal of Bio-Environment Control
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• v.27 no.4
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• pp.371-380
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• 2018

Aim of this study was to investigate the effects of different nutrient solutions and various light qualities generated by LED on the growth and glucosinolates contents of watercress (Nasturtium officinale) grown under hydroponics for 3 weeks. The seeds of watercress were sown on crushed rockwool media and raised them for two weeks. They were transplanted in a semi-DFT (deep flow technique) hydroponics system. A controlled-environment room was maintained at $20{\pm}1^{\circ}C$ and $16{\pm}1^{\circ}C$ temperatures and $65{\pm}10%$ and $75{\pm}10%$ relative humidity (day and night, respectively), with a provided photosynthetic photon flux density (PPFD) of $180{\pm}10{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and a photoperiod of 16/8h. To find out the best kinds of nutrient solutions for growing watercress, Otsuka House 1A (OTS), Horticultural Experiment Station in Korea (HES), and Netherland's Proefstaion voor Bloemisterij en Gasgroente (PBG) were adapted with initial EC of $1.0-1.3dS{\cdot}m^{-1}$ and pH of 6.2, irradiating PPFD with fluorescent lamps (Ex-1). Either monochromatic (W10 and R10) or mixed LEDs (R5B1, R3B1, R2B1G1, and W2B1G1) were irradiated with a differing ratio of each LED's PPFD to understanding light quality on the growth and glucosinolates contents of watercress (Ex-2). Although significant difference in the shoot growth of watercress was not found among three nutrient solutions treatments, but the root fresh weight increased by 13.7% and 55.1% in PBG and OTS compared to HES, respectively. OTS increased the gluconasturtiin content by 96% and 65% compared to PBG and HES. Compared with the white light (W10), the red light (R10) showed a 101.3% increase in the shoot length of watercress. Increasing blue light portion positively affected plant growth. The content of total glucosinolates in watercress was increased by 144.5% and 70% per unit dry weight in R3B1 treatment compared with R2B1G1 and W10 treatments, respectively. The growth and total glucosinolates contents of the watercress were highest under R3B1 among six light qualities.