• Proceedings of the Materials Research Society of Korea Conference
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• 2009.11a
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• pp.12.2-12.2
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• 2009

The work describes anoptimized process to highly efficient and convenient preparation in highthroughput magnetic human DNA separation with chemically functionalizedsilica-coated magnetic nanoparticles. The effect of nanoparticle's size and the surface's hydrophilicity change were studied for magnetic DNA separation process, inwhich the optimum efficiency was explored via the function of the amino-groupnumbers, particle size, the amount of the nanoparticles used, and theconcentration of NaCl salt. The DNA adsorption yields were high in terms of theamount of triamino-functionalized nanoparticles used, and the average particlesize was 25 nm. The adsorption efficiency of aminofunctionalized nanoparticleswas the 4-5 times (80-100%) higher compared to silica-coated nanoparticles only(10-20%). DNA desorption efficiency showed an optimum level of over 0.7 M ofthe NaCl concentration. To elucidate the agglomeration of nanoparticles afterelectrostatic interaction, the Guinier plots were calculated from small angleX-ray diffractions in a comparison of the results of electron diffraction TEM,and confocal laser scanning microscopy. Additionally, the direct separation ofhuman genomic DNA was achieved from human saliva and whole blood with highefficiency.

• Journal of Oral Medicine and Pain
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• v.33 no.3
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• pp.229-240
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• 2008

Animal mucins have structural characteristics similar to human salivary mucins. Animal mucins have been regarded as suitable substances for saliva substitutes. Since animal mucin molecules in saliva substitutes and host-derived antimicrobial salivary molecules exist simultaneously in whole saliva and the pellicles of patients with dry mouth, interactions may occur between these molecules. The purpose of this study was to investigate the influence of animal mucins on peroxidase activity in solution and on the surface of hydroxyapatite(HA) surfaces. The effects of animal mucins on peroxidase activity were examined by incubating porcine gastric mucin(PGM) or bovine submaxillary mucin (BSM) with either bovine lactoperoxidase(bLPO) or saliva samples. For solid-phase assays, immobilized animal mucins or peroxidase on three different HA surfaces(HA beads, HA disc, and bovine tooth) were used. Peroxidase activity was determined with an NbsSCN assay. The obtained results were as follows: 1. PGM enhanced the enzymatic activity of bLPO in solution phase. PGM did not affect the enzymatic activity of peroxidase in saliva sample(POS). 2. BSM did not affect the enzymatic activities of both bLPO and POS in solution phase. 3. HA-adsorbed PGM increased subsequent bLPO adsorption in all three HA phases. The activity of POS was increased on both the HA beads and bovine tooth. 4. The peroxidase activities on the HA beads and disc were increased when the HA surfaces were exposed to a mixture of bLPO and PGM. 5. The binding affinity of bLPO to PGM was greater than that of bLPO to BSM. Collectively, our results suggest that animal mucins affects the enzymatic activity of peroxidase on the HA surfaces as well as in solution. Saliva substitutes containing animal mucins may affect the function of antimicrobial components in natural saliva and saliva substitutes.

• Korean Journal of Microbiology
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• v.21 no.3
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• pp.143-148
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• 1983

The occurrence of PZ-peptidase in Streptococcus sanguis and other oral bacteria was investigated utilizing washed whole cells as the enzyme source and PZ-pentapeptide as its substrate. Under the culture conditions employed in the present study. Streptococcus sanguis strains, fresh isolates as well as laboratory strains, produced a broad range of the enzyme activity (0.5-7.9 unit/mg protein). The strains of both Streptococcus mutans and Lactobacilli showed low levels of activity (0-0.5 unit/mg protein for S. mutans). As compared with the enzyme activities of other bacteria, a moderate range of activity was produced by the strains of Strptococcus mitis nad Strptoccus salivarius. Actinomyces strains, like those of S. sanguis, produced a varying amount of activity (0-9.8 unit/ mg protein). A possible involvement of the oral bacterial PZ-peptidase in the metabolism of human saliva proteins is discussed.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.14
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• pp.5773-5777
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• 2015

Background: Oral squamous cell carcinoma (OSCC) is thought to develop from precancerous dysplastic lesions through multistep processes of carcinogenesis involving activation of oncogenes and loss of tumor suppressor genes. The human epidermal growth factor receptor 2 (Her-2/neu [erbB-2]), a cell membrane glycoprotein, is a growth factor receptor that has receptor tyrosine kinase activity. Her2/neu activation plays a central role in cell proliferation and survival. It has been shown that overexpression of Her2/neu increases the rate of cell division and growth, leading to precancerous changes. The aim of the present study was to compare the serum and salivary Her2/neu levels between cases with premalignant and malignant oral lesions. Materials and Methods: Fasting blood samples and unstimulated saliva by passive drooling were collected from three groups of healthy control (n=20), premalignant disorder (PMD) (n=20) and OSCC (n=25) subjects. The HER2 extracellular domain (HER2 ECD) levels were measured using ELISA. Results: The levels of serum Her2/neu showed no significant differences between any of the groups but on the other hand salivary Her2/neu levels were found to be significantly (p<0.05) higher when compared between control (median 68.7 pg/ml, range: 21.5 - 75.8) and OSCC (median 145.6 pg/ml, range: 45.1-191.1). A similar trend was observed when comparing between PMD (median 43.3, range: 22.1 -94.7) and OSCC with a statistical significance of p<0.05. Conclusions: Our study provided evidence of increased salivary Her2/neu in OSCC when compared to PMD and control which was not the case for serum levels. This suggests that probably Her2/neu is not highly amplified as in breast cancer so as to be reflected in serum. Since saliva is in local vicinity of the OSCC, even a mild increase might be mirrored. On the whole, this study proposes Her2/neu as marker for distinguishing premalignant and malignant conditions.

• Journal of Oral Medicine and Pain
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• v.22 no.2
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• pp.283-294
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• 1997

타액은 분비율과 그 구성성분으로 인해 구내환경을 조절하는데 있어 가장 중요한 요인으로 여겨진다. 타액 분비율과 성분에 관한 많은 연구가 이루어 졌지만, 증령이 타액과 그 성분에 미치는 영향에 대한 연구는 상반된 결과를 보고하고 있으며 현재까지도 논란의 여지가 많다. 또한 증령에 따른 lactoferrin과 전해질의 변화는 거의 보고되지 않은 실정이다. 이에 저자는 증령이 타액분비량과 타액성분에 미치는 영향을 연구하기 위하여 59명의 투약력이 없고 건강한 사람을 대상으로 연구를 시행하였다. 연구대상을 그들의 나이에 따라 A군, 10~15세 (남자7명, 여자7명); B군,20~30세 (남자8명, 여자7명 ); C군,40~50세 (남자7명, 여자7명 ); D군,60세 이상 (남자7명, 여자9명 ) 등의 4군으로 구분하여 각각의 비자극성 전타액을 표준화된 방법으로 채취한후 타액분비량과 immunoglobulin, lactoferin 및 전해질의 변화를 측정하였다. 이와 같은 실험을 통해 다음과 같은 결론을 얻었다. 1. 비자극성 타액분비량은 각 연령군간의 유의한 차이가 관찰되지 않았으며, 20-30세 군(B군)에서만 남성에 비해 여성에서 유의하게 낮았다. 2. 인체 전타액내 IgA와 lactoferin 농도는 연령이나 성별에 따른 뚜렷한 변화는 없었지만, 10-l5세 군(A군) 남성에서 유의하게 낮았다. 3. 인체 전타액내 IgG의 농도는 연령이나 성별에 따른 차이가 관찰되지 않았다. 4. 인체 전타액내 IgM의 농도는 60세이상 군(D군) 남성에서 유의하게 낮은 농도를 보였다. 5. 인체 전타액내 전해질(sodium, chloride, potassium, magnesium)의 농도는 증령에 따라 증가하는 경향을 보였다. magnesium과 chloride는 60세이상 군(D군)에서, sodium과 potassium은 40-50세 군(C관)에서 최대치를 보였다 성별간의 유의성 있는 차이는 발견되지 않았다.

• The Journal of Korean Medicine
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• v.16 no.2 s.30
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• pp.453-489
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• 1995

The research of the phrases related with physiology of stomach and spleen in the contents of Huang Di Nei Jing(黃帝內經) known as the Bible of oriental medicine will make a contribution to a deep understanding of disease of stomach and spleen and a proper clinical diagnosis and treatment of them. In this research of the most appropriate glosses recorded nine kinds of representative medical books including Huang Di Nei Jing Somoon(黃帝內經素問) of Wang Bing(王氷) were picked out: The summaries of the selected contents are as follows: 1. The word 'saliva(涎)' in 'the spleen controls saliva(脾爲涎)' can be viewed as a generic term referring to oral cavity secretion gland as well as the secretion fluid of salivary gland. 2. The phases 'a large reservoir(太倉)', barn organs', 'a reserboir of food stuff', 'a stomach as the market(胃爲之市)', etc mean the function of stomach to receive food(胃主受納). 3. The phase 'generation of five tastes(五味出焉)' means both 'the function of stomach to transform food into chyme(胃主腐熟)' and 'the channelling function of spleen.(脾主運化)' 4. The flowing of the food-Qi(食氣) into stomach brings about spreading Jung(精) into liver and then percolating Jung(精) flow into channel. The channel-Qi(脈氣) flows into lung through channel. As a result, all kinds of channels gather together in lung and Jung(精) is sent into skin and hair. The assembly of Jung(精) with skins and channels moves Qi(氣) into fu-organ and so jung(精) and mental activity(神明) in fu-organ(府) come to be in four organs(四臟). Then if Qi(氣) comes back to power balance unit(權衡) being in the state of equilibrium(權衡以平), the hole of Qi(氣口) comes to determine the matter of life and death through achieving Chun-quan-chi(-寸-關-尺). The above mentioned phrases means the digestion, asorption and transmission of food. When food is taken in stomach, Jung-Qi(精氣) comes to be over flowed upward into spleen, back into lung, finally downward into bladders through water-conduit(水道) controlled by lung. When water- Jung(水精) radiates into whole body with channels of five organs(五臟), both of them fit together with and yin-yang(陰-陽). Therefore, the grasping of the rise and decline of yin-yang(陰C-陽) is necessary to consult patients. The above mentioned phrases is properly viewed to designate the asorption, transmission and excretion of food. 5. Spleen controls flesh(脾之合肉也), the state of spleen is known by human lips, and what this means is that liver plays functions of spread and expansion(肝主疏泄). 6. The phrase 'Jung Jung'((中精)) in 'gallbladder dominates Jung jung(膽主中精)', which in one of the specific expression of 'liver plays functions of spread and expansion(肝主疏泄). 7. It is right that the phase 'The eleven organs in all are determined by gallbladder'(凡十,一臟取決於膽也) is correctly paraphrased as 'only one of ten organs, spleen, is determined by gallbladder'.(凡十,一臟取決於膽也), 8. The small intestine is an organ. which receives the materials digested and sends them out. This means that the function of transforming materials(化物) factually refers to that of separating clearity and blur(泌別淸濁). And it is also thought to have the function of ascending clearity and descending blur(升淸降濁), 9. A large intestine is a transmitting organ(傳導之官) from which a change comes out(變化出焉). the phrase 'change'(變化) in this sentence means both the intake of water and nutrition and the formation procedure of stool through excretion of mucocele.