• 동의생리병리학회지
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• 제18권2호
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• pp.553-560
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• 2004

In the present study, we investigated the anti-proliferative effects of aqueous extract of Wikyung-tang(WKT) on the growth of human lung carcinoma cell line A549. WKT treatment declined the cell viability and proliferation of A549 cells in a concentration-dependent manner. The anti-proliferative effects by WKT treatment in A549 cells was associated with morphological changes such as membrane shrinking and cell rounding up. WKT treatment induced an inhibition and/or degradation of apoptotic target proteins such poly(ADP-ribose) polymerase (PARP) and phospholipase C-γ1 (PLC-γ1). WKT treatment did not affect the levels of other Bcl-2 family gene products, such as Bcl-2, Bax and Bad. Western blot analysis and RT-PCT data revealed that the levels of tumor suppressor p53 and cyclin-dependent kinase inhibitor p21 were induced by WKT treatment in A549 cells. Additionally, WKT treatment induced the down-regulation of telomerase reverse transcriptase mRNA (hTERT) expression of A549 cells, however, the levels of other telomere-regulatory gene products were not affected. Taken together, these findings suggest that WKT-induced inhibition of human lung cancer cell proliferation is associated with the induction of apoptotic cell death via regulation of several major growth regulatory gene products and WKT may have therapeutic potential in human lung cancer.

• Animal cells and systems
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• 제16권5호
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• pp.366-375
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• 2012

Gallotannin (GT) is derived from plant poly phenol and is associated with biological actions in a wide range of cells. In this study, we evaluated the effect of GTon apoptosis and cyclooxygenase-2 (COX-2) expression and attempted to shed light on the mechanism of action in A549 human lung carcinoma cells. We found that GT dramatically induced apoptosis as demonstrated by expression of p53 and active caspase-3 via western blot analysis and fragmented DNA as detected by DNA fragmentation and DAPI staining. We also observed that GT significantly causes COX-2 expression in a dose-dependent manner determined by western blot analysis. Phosphorylation of Akt and p38 was considerably increased by GT in A549 human lung carcinoma cells. Inhibition of Akt and p38kinase with LY294002 or SB203580 suppressed GT-induced apoptosis and COX-2 expression. Furthermore, we have shown that prevention of COX-2 with NS398 or indomethacin does not any effects on apoptosis induced by GT. Taken together, our present results suggest that GT regulates apoptosis and COX-2 expression through Akt and p38kinase pathway in A549, human lung carcinoma cells.

• 한국환경보건학회지
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• 제5권1호
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• pp.18-20
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• 1978

An experimental study was conducted to determine the quantity of contamination of organophosphrous pesticides accumulated in each human tissues. The samples used for this experiment were spleen, lung, heart, liver and kindney and those tissues were homogenized by a blender. The homogenized materials was extracted with mixed solvent, acetone/benzene (1:1) and cleaned up on a activated carbon column and determined by gas chromatography using AFID supported on 5% QF-1. The average recovery rate was 94% and the results obtained are summarized as follows. 1) The quantities of Malathin accumulated in each tissues were 0.53 ppm in spleen, 0.42 ppm in lung, 0.34 ppm in kidney, 0.19 ppm in heart and 0.19 ppm in liver. 2) Residues of pesticides in chronic or acute intoxicated tissues were highest in the spleen, decreasing in order of the lung, kidney, heart, and liver. 3) According to the above resuk we can conclude that the most proper material in detecting the pesticide is the spleen.

• 한국식품영양과학회지
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• 제29권5호
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• pp.870-874
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• 2000

This study was designed to investigate the cytotoxic effect of methanol extract of garlic, onion and those mixture on two kinds of human lung cancer cell lines (NCI-H522, NCI-H596) using MTT assay. MeOH extract of garlic, onion and those mixture showed cytotoxic effect on both NCI_H522 and NCI-H596. The growth of the cancer cells exposed to medium containing garlic, onion extracts and those mixture was inhibited dose-dependently. The growth of NCI-H522 was inhibited more in the garlic extract than in the onion extract, but that of HCI-H596 was inhibited highese in the onion extract. IC50 values of garlic extract on NCI-H522 and NCI-H596 were 0.84 mg/mL, 0.88 mg/mL and those of onion extract were 1.04 and 0.79, respectively. and the mixture of garlic and onion extracts also inhibited the growth of both NCI-H522 and NCI-H596 cells.

• Journal of Chest Surgery
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• 제17권1호
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• pp.94-100
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• 1984

The purpose of present study is to investigate the significance of preoperative evaluation with perfusion lung scintigraphy in patients with lung cancer. Lung scans with the use of macroaggregated human serum albumin labeled with technetlum-99m were carried out in 35 patients with lung cancer before thoracotomy at Seoul National University Hospital during the period from November 1981 to September 1983. The relationship between size of the perfusion defect as seen by perfusion lung scan and size of the mass lesion as seen radiologically was correlated with the presence of regional adenopathy and resectability. Among patients with a larger perfusion defect than mass lesion on chest X-ray film.86% were found to have regional lymph node involvement with 29% resectability, whereas among patients in whom a larger defect was not present only 14% had such extension of the disease with 93% resectability. The relative pulmonary arterial perfusion of affected lung was calculated from the counts of radioactivity recorded from affected lung on both anterior and posterior scans expressed as a percentage of the total counts in the scan. The mean relative pulmonary arterial perfusion of the inoperable group [34\ulcorner%] is significantly different from both that of the pneumonectomy group [39\ulcorner%] and that of the lobectomy group [48\ulcorner%].(p<0.01)

• Journal of Pharmaceutical Investigation
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• 제31권2호
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• pp.73-80
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• 2001

Lung perfusion scanning, invariably combined with ventilation studies provides a reliable and non-invasive mean to diagnose lung related pathologies despite the availability of modern techniques such as angiography, magnetic resonance imaging, magnetic resonance angiography, and helical (spiral) computed tomography. The technique involves the generation of images by radiations emitted from radioisotopes introduced in to the lungs. Various radiopharmaceuticals have been proposed and designed to incorporate $Tc^{99m}$ in to macroparticulate form for lung perfusion imaging. However, most of these have associated difficulties such as reproducibility of the product with regards to particle size distribution and poor elimination from the lung capillary bed. $Tc^{99m}$ macroaggregated albumin $(Tc^{99m}-MAA)$ is used extensively for clinical lung perfusion imaging and is considered as the radiopharmaceutical of choice. It is non-toxic, safe, and being biodegradable, is easily eliminated from the lung capillary bed by proteolytic enzyme metabolism and by mechanical forces due to lung movement.

• 한국화재소방학회논문지
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• 제22권2호
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• pp.121-128
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• 2008

화재에 의한 흡입연기의 중장기 인체 유해성은 흡입연기가 폐에 침착되는 양과 밀접한 관련이 있다. 연기의 폐 내 침착량을 구하기 위해서는 인체 실험이 불가능한 만큼 폐모델을 이용한 실험이 필요하나 실제 폐형태에서 나타나는 연속적으로 감소되는 분지관의 제작상 어려움으로 인하여 하위 세대에서는 모델실험을 통한 침착 연구가 힘들다. 본 문제를 해결하기 위하여 이 연구에서는 아래로 갈수록 직경이 단계적으로 감소하는 구형 충전층을 이용한 폐모델을 개발하고 이를 폐침착 실험에 적용하였다. 실험장치는 각 입자크기별 호흡 패턴 변화에 따른 입자의 침착량을 측정하도록 구성되었으며 표준입자에 대한 실험 값을 실제 폐에 대한 결과와 비교함으로써 개발된 폐모델의 타당성을 검증하였다. 이 폐모델은 화재시 발생하는 여러 가지 연기입자의 흡입에 의한 인체 피해 연구에 도움이 될 것으로 생각된다.

• 대한의생명과학회지
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• 제29권4호
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• pp.336-343
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• 2023

Lung cancer is one of the cancers with high mortality and incidence rates worldwide. Although, various anticancer research efforts are underway to completely treat cancer, the challenge against it remains in the inability to eliminate cancer stem cells (CSCs), leading to difficulties in curing the cancer and resulting in recurrence. As a result, there is a growing interest in the discovery of new biomarkers and therapeutic molecules that can simultaneously target both cancer cells and CSCs. From this point of view, we focused on fibronectin leucine rich transmembrane protein 3 (FLRT3), one of the genes known to be present in human lung cells and the discovery from our previous cancer proteomic analysis study. This study aimed to evaluate the potential of FLRT3 as a specific therapeutic biomarker for lung cancer and Lung Cancer-derived-Stem Cells (LCSC). Also, to estimate the biological function of FLRT3 in cancer and LCSC, short hairpin RNA (shRNA) was generated and showed the ability of the decreased-cell migration and cell proliferation of lung cancer through ERK signaling pathway when FLRT3 was knock-downed. In conclusion, our study is the first to report that FLRT3 has the potential as therapeutic biomarker for the treatment of lung cancer and LCSC.

• Bulletin of the Korean Chemical Society
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• 제35권1호
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• pp.319-321
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• 2014

• Bulletin of the Korean Chemical Society
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• 제34권4호
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• pp.1293-1295
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• 2013