• Korean Journal of Agricultural Science
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• v.47 no.3
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• pp.673-681
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• 2020

Human enteric Adenovirus 41 (HueAdV-41) is a major waterborne virus that causes human gastroenteritis and is classified as a viral group I double-strand DNA virus, Adenoviridae. HueAdV-41 has been detected with the polymerase chain reaction (PCR) in various samples such as ground water. However, the PCR-based diagnostic method has problems such as reaction time, sensitivity, and specificity. Thus, the loop-mediated isothermal amplification (LAMP) assay has emerged as an excellent method for field applications. In this study, we developed a LAMP system that can rapidly detect HueAdV-41 with high specificity and sensitivity. HueAdV-41 specific LAMP primer sets were tested through a specific, non-specific selection and sensitivity test for three prepared LAMP primer sets, of which only one primer set and optimum reaction temperature were selected. The developed LAMP primer set condition was confirmed as 63℃, and the sensitivity was 1 copy. In addition, to confirm the system, a LAMP positive reaction was developed with the restriction enzyme Taq I (T/GCC). The developed method in this study was more specific, rapid (typically within 2 - 3 hours), and highly sensitive than that of the conventional PCR method. To evaluate and verify the developed LAMP assay, an artificial infection test was done with five cDNAs from groundwater samples, and the results were compared to those of the conventional PCR method. We expect the developed LAMP primer set will be used to diagnose HueAdV-41 from various samples.

• Journal of Advanced Navigation Technology
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• v.19 no.6
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• pp.492-498
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• 2015

With the rapid growth of technologies and demand of remotely piloted aircraft systems (RPASs), integration of such systems into the existing airspace is becoming an issue in many countries. To assess the impact of integrated operations of manned and remotely piloted aircraft (RPA), it is necessary to perform Human-in-The-Loop (HiTL) simulations of likely situations with an integrated simulation system. This paper defines several operational concepts for the integrated simulation. Several probable scenarios were developed including a traffic pattern at a small airport and an altitude maneuver at a route crossing. HiTL simulations were performed according to the developed scenarios. The simulation results are analyzed focusing on the impacts of different communication, safety, performance, and human machine interface (HMI) characteristics of RPA.

• Journal of the Ergonomics Society of Korea
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• v.31 no.4
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• pp.533-540
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• 2012

Objective: This paper targets a framework of a hand gesture based interface design. Background: While a modeling of contact-based interfaces has focused on users' ergonomic interface designs and real-time technologies, an implementation of a contactless interface needs error-free classifications as an essential prior condition. These trends made many research studies concentrate on the designs of feature vectors, learning models and their tests. Even though there have been remarkable advances in this field, the ignorance of ergonomics and users' cognitions result in several problems including a user's uneasy behaviors. Method: In order to incorporate compatibilities considering users' comfortable behaviors and device's classification abilities simultaneously, classification-oriented gestures are extracted using the suggested human-hand model and closed-loop classification procedures. Out of the extracted gestures, the compatibility-oriented gestures are acquired though human's ergonomic and cognitive experiments. Then, the obtained hand gestures are converted into a series of hand behaviors - Handycon - which is mapped into several functions in a mobile device. Results: This Handycon model guarantees users' easy behavior and helps fast understandings as well as the high classification rate. Conclusion and Application: The suggested framework contributes to develop a hand gesture-based contactless interface model considering compatibilities between human and device. The suggested procedures can be applied effectively into other contactless interface designs.

• Journal of the Korean Institute of Intelligent Systems
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• v.25 no.2
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• pp.97-104
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• 2015

A fuzzy controller and a 2-dimensional wire-driven heavy material lifting system helping human operator are proposed in this paper. The 2-dimensional wire-driven heavy material lifting system is a kind of human-assistive systems in which a human is involved in the control loop. Most of the existing human-assistive control systems cannot consider human operator's characteristic. To consider human operator's characteristic, human's operating motion and requirement of reducing operator's force to lift a heavy material are considered in the design process of the proposed fuzzy controller. The performance of the proposed system is verified by experiments.

• Archives of Pharmacal Research
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• v.22 no.3
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• pp.262-266
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• 1999

Dopamine transporter (DAT) plays the most important role in terminating the actions of dopamines released into the synaptic cleft. DAT is also the target of various psychotropic drugs such as cocaine and amphetamine. In this study were prepared DAT-specific antibodies using the 2nd extracellular loop of rat DAT as an antigen. The 2nd extracellular loop of the rat DAT was expressed in bacterial as a fusion protein with glutathione-S-transferase, and injected ito rabbits to raise antibodies. Produced antibodies clearly recognized the rat DAT in ELISA, immunoblotting, and immumoprecipitation. As expected from the high sequence homology between the rat and human DAT, the antibodies raised for the rat DAT cross-reacted with the human DAT in the immunoblotting. Considering the specificity for DAT with wide range of applications such as ELISA, immunoblotting, and immunoprecipitation, these antibodies would be valuable tool for understanding the pharmacological actions of dopamine transporter and drug addition.

• Genomics & Informatics
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• v.20 no.4
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• pp.46.1-46.7
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• 2022

Influenza A virus (IAV) is the most widespread pathogen causing human respiratory infections. Although polymerase chain reaction (PCR)-based methods are currently the most commonly used tools for IAV detection, PCR is not ideal for point-of-care testing. In this study, we aimed to develop a more rapid and sensitive method than PCR-based tools to detect IAV using loop-mediated isothermal amplification (LAMP) technology. We designed reverse-transcriptional (RT)-LAMP primers targeting the hemagglutinin gene. RNAs from reference H1N1 and H3N2 showed specific RT-LAMP signals with the designed primers. We optimized the reaction conditions and developed universal reaction conditions for both LAMP assays. Under these conditions, the detection limit was 50 copies for both RT-LAMP assays. There was no non-specific signal to 19 non-IAV respiratory viruses, such as influenza B virus, coronaviruses, and respiratory syncytial viruses. Regarding the reaction time, a positive signal was detected within 25 min after starting the reaction. In conclusion, our RT-LAMP assay has high sensitivity and specificity for the detection of the H1 and H3 subtypes, making it suitable for point-of-care IAV testing.

• Journal of Mechanical Science and Technology
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• v.19 no.11
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• pp.1998-2006
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• 2005

The lane departure avoidance systems have been considered promising to assist human drivers in AVCS (Advanced Vehicle Control System). In this paper, a lane departure monitoring and control system is developed and evaluated in the hardware-in-the-loop simulations. This system consists of lane sensing, lane departure monitoring and active steering control subsystems. The road image is obtained based on a vision sensor and the lane parameters are estimated using image processing and Kalman Filter technique. The active steering controller for avoiding the lane departure is designed based on the lane departure metric. The proposed lane departure avoidance system is realized in a steering HILS (hardware-in-the-loop simulation) tool and its performance is evaluated with a driver in the loop.

• Journal of Information Processing Systems
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• v.15 no.3
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• pp.593-603
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• 2019

In wearable healthcare systems, sensor devices can be deployed in places around the human body such as the stomach, back, arms, and legs. The sensors use tiny batteries, which have limited resources, and old sensor batteries must be replaced with new batteries. It is difficult to deploy sensor devices directly into the human body. Therefore, instead of replacing sensor batteries, increasing the lifetime of sensor devices is more efficient. A transmission power control (TPC) algorithm is a representative technique to increase the lifetime of sensor devices. Sensor devices using a TPC algorithm control their transmission power level (TPL) to reduce battery energy consumption. The TPC algorithm operates on a closed-loop mechanism that consists of two parts, such as sensor and sink devices. Most previous research considered only the sink part of devices in the closed-loop. If we consider both the sensor and sink parts of a closed-loop mechanism, sensor devices reduce energy consumption more than previous systems that only consider the sensor part. In this paper, we propose a new approach to consider both the sensor and sink as part of a closed-loop mechanism for efficient energy management of sensor devices. Our proposed approach judges the current channel condition based on the values of various body sensors. If the current channel is not optimal, sensor devices maintain their current TPL without communication to save the sensor's batteries. Otherwise, they find an optimal TPL. To compare performance with other TPC algorithms, we implemented a TPC algorithm and embedded it into sensor devices. Our experimental results show that our new algorithm is better than other TPC algorithms, such as linear, binary, hybrid, and ATPC.

• IEMEK Journal of Embedded Systems and Applications
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• v.9 no.4
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• pp.211-217
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• 2014

Cyber-Physical Systems(CPS) that mostly provides safety-critical and mission-critical services requires high reliability, so that system testing is an essential and important process. Hardware-In-the-Loop Simulation(HILS) is one of the extensively used techniques for testing hardware systems. However, most conventional HILS has problems that it is difficult to support a distributed operating environment and to reuse a HILS platform. In this paper, we introduce EcoHILS(ETRI CPS Open Human-Interactive hardware-in-the-Loop Simulator) in order to test CPS effectively. Moreover, feasibility tests and performance tests of EcoHILS are performed to confirm its effectiveness.

• Journal of the Korean Society of Safety
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• v.36 no.5
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• pp.10-17
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• 2021

The controversy over the risk of the human body being affected by electromagnetic fields emitted from 60 Hz power lines continues without end. There are currently no new studies or research progress being made in this direction that is notable, and the number of civil complaints is gradually increasing. The problem is that each study produces different results, among which the effect of exposure to magnetic fields on childhood leukemia is a major one. In Korea, an electrician who was maintaining a 22.9 kV power line died of leukemia, which has recently been recognized as an occupational disease. Methods to reduce magnetic fields from power lines include shielding with wire loops, incorporating split phases and compaction techniques, installing underground power lines, converting to high-voltage direct current (HVDC), and increasing the ground clearance of transmission towers. Depending on whether a separate power supply is needed or not, there are two types of wire loops: passive loop and active loop. Magnetic field reduction is currently done through underground power lines; however, the disadvantage of this process is high construction costs. Installing passive loops, with relatively low construction costs, leads to lower magnetic field reduction rates than installing underground cables and a weakness to not solving the landscape problem. This methodological study aims at designing methods and reducing the effects of 2-wire set loops-the simplest and most practical. Since the method proposed in this study has been designed after analyzing the distribution of complex electromagnetic fields near the expected loop installation location, a practical design can be implemented without the need for any difficult optimization programming.