• The korean journal of orthodontics
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• v.26 no.6
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• pp.667-676
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• 1996

We have examined the in vitro stage-related chondrogenic potential of human mandibular and limb bud mesenchyme cells using micromass culture. Our results indicate that limb bud mesenchyme cells as early as stage 16 by Carnegie system (37 days), well before the initiation of in vivo chondrogenesis, have chondrogenic potential which is expressed in micromass culture. These results are correlated with stage-related chondrogenic potential of human limb bud in vivo as a result of Alcian blue staining. The proliferation of chondrogenic cells increased in the first 3 days after culture and then decreased. These results were correlated with the cell cycle analysis of which the number of $G_0^1/G_1$ phase increased markedly after 3 days of culture, while the percentage of cells in S phase was decreased. On the other hand, it was rarely differentiated in the mandible. We examined the effects of two PKC modulators such as phorbol 12-myristate 13-acetate (PMA), a potent activator of PKC, and staurosporine (STSN), an inhibitor of PKC. PMA inhibited the chondrogenesis, whereas STSN promoted the chondrogenesis in a dose dependent manner. In addition, PMA exerted no inhibitory effect when the cells were pretreated for 24 h with STSN, implying that the chondrogenic events might be settled at an early step in vitro and FKC may act as a negative modulator. Collectively, these results demonstrate, for the first time, the stage-related chondrogenic potential of human mandibular and limb bud mesenchyme cells and the role of PKC during chondrogenesis in vitro & in vivo.

• Proceedings of the Korean Institute of Interior Design Conference
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• 2007.05a
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• pp.204-209
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• 2007

As people are getting interested in themselves ever than before, the value of culture industry which can satisfy their emotions has been considered much more important. According to this, in terms of the 'space', it is better to represent 'space' not just as a functional space, but the human-centered and making human and nature together. In this society, the most human-centered design can be achieved by pursuing value which is friendlier to nature. And it is sure that the interior design that completes life quality as well as emotions of human will be common in near future. There already has been a try 'Green interior' in subway station in variety ways, and now it is needed to make indoor display in Seoul subway station different in order to satisfy human emotions. If culture space in Seoul subway station applied eco-element interacts with indoor environment, it is surely expected that people have better feeling on subway station as well as help eliminate negative elements which underground have had. In culture space, there are many advantages for displaying space into 'nature', that is, natural space represents the best comfortable and agreeable space, and future-oriented design when it gets interacted with environment. Application of 'Green interior' in Seoul subway station makes special place ness by its symbol and image, so that it derives people to have more natural and comfortable movement distance in subway station, offers more comfortable and cozy place as well. Furthermore, it makes people very moved by experiencing the most special culture space that they have ever had.

• Journal of the Ergonomics Society of Korea
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• v.33 no.2
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• pp.155-165
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• 2014

Objective: The aim of this study is to develop an office chair enabling to keep working at reclined sitting posture. Background: Sedentary workers are supposed to change the posture frequently during long hours of sitting. A reclined sitting position has been recommended to reduce disc pressure. But slumped sitting posture caused by the buttock sliding forward without any adjustment of back reclining is commonly observed. The worker seems to have tendency to change the sitting posture maintaining working condition. We assumed the reason to be their hands movement away from the working space when tilting backward. Method: Slide mechanism allowing seat to move forward was designed to maintain the hand position in working space during reclining. A prototype was manufactured and tilting motion was analyzed using motion capture system. Four experiment chairs were tested including the manufactured prototype chair and three other commercial chairs. Results: A backward movements of the hand position were 13.0mm, 101.7mm, 156.1mm and 139.3mm at the prototype chair, compared to chair B, chair C and chair D, respectively. And the movement was remarkably small at the prototype chair. Conclusion: The developed seat sliding chair allows back tilting maintaining hand position at working space. We expect the user tilting back more often than normal tilting chair during seated work. But further investigation is required to figure out the effectiveness of the developed chair using prolonged working hours. Application: The developed office chair directly affects commercialization.

• Journal of Pharmaceutical Investigation
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• v.32 no.1
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• pp.21-26
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• 2002

The primary culture of human nasal epithelial cell monolayer was performed on a Transwell. The effect of various factors on the tight junction formation was observed in order to develop an in vitro experimental system for nasal transport studies. Human nasal epithelial cells, collected from human normal inferior turbinates, were plated onto diverse inserts. After 4 days, media of the apical surface was removed for air-liquid interface (ALI) culture. Morphological characteristics was observed by transmission electron microscopy (TEM). A polyester membrane of $0.4\;{\mu}m$ pore size was determined as the most effective insert based on the change in the transepithelial electric resistance (TEER) value as well as the $^{14}C-mannitol$ transport study. The ALI method was effective in developing the tight junction as observed in the further increase in the TEER value and reduction in the permeability coefficient $(P_{app})$ of $^{14}C-mannitol$ transport. Results of the transport study of a model drug, budesonide, showed that the primary culture system developed in this study could be further developed and applied for in vitro nasal transport studies.

• Journal of Korean Library and Information Science Society
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• v.5
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• pp.1-31
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• 1978

This thesis is to discuss and prove the identity of the acts of the library and the character of the library which regards these as its objects. And this study is analyzed in view of per ceptual problems and scientific problems and scientific problems, in order to make clear the character of the acts of the library. In these processes, some basic propositions are extracted. First, culture can be found in arranged order. Second, human being can create culture by the acquisition of accmulated culture. Third, an individual is purposely related with culture. Fourth, culture is antonomous to mankind. And in this thesis, library science is defined as the systematic formation of mankind's pursuing acts, and to identify this definition, some theoretical models of library science have been offered as follows : Model I 1. Theory of making culture assets informative. 2. Theory of making culture assets empirical; that of imformation treatment. 3. Theory of medial acts of librarians. Model II 4. Theory of organic systems of libraries. Model III 5. Theory of guiding school activities. 6. Theory of extending the inhabitants' interests of communities. 7. Theory of a kind of resources in social improvement. Model IV 8. Theory of a means of principal approach to the succession of culture. In conclusion, this study is to prove that acts of libraries are the universal ones of those human being that are gualified to be the unique means to the creation of culture and, library science is a science that shapes concepts and rules to describe, to make explanation and to foretell these acts.

• The Research Journal of the Costume Culture
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• v.26 no.6
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• pp.934-950
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• 2018

This study suggests a new perspective for designing men's tailored jackets by more carefully considering human muscle structure. For this study, we examined research regarding the construction of the tailored jacket that is based on costume history references, as well as research regarding human muscle structure that is based on human anatomy references and the analysis of recent fashion designs illustrating the human body image. Based on this research, we developed various tailored constructions that account for human muscle structure. These constructions are applied primarily to the backs of four tailored jackets, as the back of the jacket needs a mechanism to accommodate the wearer's movement. The following conclusions have been derived from the study: First, by developing the tailored garment structure that accounts for the muscle structure of the human body, we suggest a new design direction for tailored garments. Second, we propose a new type of tailored jacket structure for the back of the jacket that incorporates an artificial muscle structure to accommodate the wearer's activities. This new type of jacket indicates the potential for designs that use structure, particularly the structure of the human body. Finally, by using the embroidery technique, we changed the texture of the material into the shape of human muscle. Thus, we propose a design that uses three-dimensional volume to accounts for the shape of human body tissue.

• Journal of Embryo Transfer
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• v.19 no.1
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• pp.11-18
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• 2004

The aim of this study was to evaluate the improvement of testicular sperm motility following different culture conditions such as human follicular fluid (hFF) and temperature. Testicular tissues obtained from azoospermia (n=21) were minced into small pieces by blade and recovered sperm suspension were cultured in Ham's F10 with or without 40% hFF at different temperatures (Group I: 37$^{\circ}C$/with hFF, Group II: 32$^{\circ}C$/withGroup III: 37$^{\circ}C$/without, Group IV:32$^{\circ}C$ /without The motility and viability of sperm were monitored during culture for 48 hours. Initial motility of testicular sperm was 10.9$\pm$1.9%. After 24 hours culture, sperm motility was 23.5$\pm$2.1% (Group I), 8.1$\pm$1.1% (Group II), 10.4$\pm$ 1.4% (Group III) and 4.0$\pm$0.8% (Group IV), respectively. After 48 hours, the motility had been changed as 32$\pm$2.3% (Group I), 14.3$\pm$1.7% (Group II), 5.3 $\pm$1.4% (Group III) and 4.3$\pm$0.9% (Group IV). In hFF group (I and II), sperm motility of group I cultured at 37$^{\circ}C$ was higher than those of group II at 32$^{\circ}C$. But, sperm viability of group I cultured at 37$^{\circ}C$ was lower than those of group II at 32$^{\circ}C$ (54.4$\pm$4.1% vs. 59.4$\pm$3.7%) after cultured for 48 hours. We acquired the best motility of testicular sperm when performed in vitro culture for 48 hours in hFF supplemented medium at 37$^{\circ}C$. Increase of sperm motility by in vitro culture could be useful tool fur human TESE-ICSI program.

• International Journal of Advanced Culture Technology
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• v.5 no.1
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• pp.26-31
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• 2017

In this study, Emotion-DNA is constructed in the same way asthat the human DNA constructs the human body. Emotion-DNA is copied and translated in the same way as that the human DNA copies and translates itself. We made an attempt to embody the mind by Emotion-DNA like the symbols "A, T, G, C, U" that make up the chromosome of the human body. This is a diagram of the flow of emotions that the human body operates by literary works. These schemes present new directions for the therapeutic analysis of literary works and for the creation of therapeutic literary works. In this study, we analyzed the nominal Emotion-syllables as a framework of the structuring of emotional DNA. As a result, through the structuring of the emotional DNA, it was judged that the therapeutic action of the human body, which is included in the Rated Sijo among the literary works, can be more concrete and powerful than the works of other genres.

• Journal of the Korea Furniture Society
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• v.21 no.1
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• pp.1-16
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• 2010

Furniture is a medium to interconnect the living space to human, and it is the closest environmental element that limits the functions of the space, defines the style of the living, and adjusts the actions and behaviors of human. Therefore the furniture can be said to be one of the craft arts that reflects the culture and living style as they are according to the periodical, regional, and social stratum characteristics. Within this context, this study tries to suggest new design and living culture that can harmonize with the modern residence living culture and incorporate the traditional varnishing technique with lacquer that had been discontinued. Coming to the modern days, the varnishing with lacquer is getting attention again with its environment friendly characteristics and antibiotic functions, and is being applied to various applications. To inherit and maintain such an excellent traditional culture, I would like to improve the practicality by applying to modern furniture, and to contribute to develop and tell the world about the superiority of our culture.

• Proceedings of the Costume Culture Conference
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• 1999.06a
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• pp.5-10
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• 1999