• Proceedings of the KSR Conference
• /
• 2008.11b
• /
• pp.2040-2044
• /
• 2008

Recently, many safety measures are developing for the prevention of human error, which is main factors of railway accident. For the efficient management of human factors, many expertise on design, conditions, safety culture and staffing are required. But current safety management activities on safety critical works are focused on training, due to the limited resource and information. In order to establish railway human factors management, a systematic review model is required. Based on system engineering and nuclear industry model, a program review model is proposed in this study. The model includes operating experience review, task analysis, staffing and qualification, human reliability analysis, huma-system interface design, procedure development, training program, verification and validation, implementation and monitoring. Results can be applied for the review of safety measures relating to human factors.

• Journal of the Korea Society of Computer and Information
• /
• v.24 no.2
• /
• pp.201-207
• /
• 2019

The purpose of this study is to suggest some implications by empirical testing the relationships between children's self-perception and human rights recognition in local children's center. For this study, Total 438 children were sampled and surveyed. The analytical results are the followings. First, personal characteristics was shown to have a significant effect to human right recognition. Second, self-perception was shown to have a significant effect to human right recognition. Third, right guarantee of center was shown to moderate the relationship between self-perception and human right recognition. This study provides some theoretical and polcy implications basing on these analytical results.

• Journal of Periodontal and Implant Science
• /
• v.32 no.2
• /
• pp.389-402
• /
• 2002

BMP can induce ectopic bone formation when implanted into sites such as rat muscle and can greatly enhance healing of bony defects when applied exogenously. In addition, BMP stimulated osteoblastic differentiation in vitro in various types of cells. The aim of this study was to investigate the effect of recombinant human bone morphogenetic protein(rhBMP-2) on the proliferation and osteoblastic differentiation of human periodontal ligament cells and gingival fibroblasts. The cell number and alkaline phosphatase activity were measured in 3 experimental groups of human periodontal ligament cells and gingival fibroblasts (control group, rhBMP-2 50ng/ml group, and rhBMP-2 100ng/ml group) at 1 and 2 weeks after culture. At the same time, total RNA of cultured cells were extracted and reverse trascription polymerase chain reaction(RT-PCR) was performed to determine the expression of mRNA of bone matrix protein. RhBMP-2 had no effect on the cell proliferation of human periodontal ligament cells and gingival fibroblasts. Alkaline phosphatase activity was elevated significantly by rhBMP-2 in both cells. And periodontal ligament cells showed significantly higher alkaline phosphatase activity than gingival fibroblasts. ${\beta}$-actin, type I collagen, alkaline phosphatase, BMP-2 mRNA were expressed in all of the samples. Osteopontin, osteocalcin mRNA were expressed in all periodontal ligament cell groups, and rhBMP-2 50ng/ml group, rhBMP-2 100ng/ml group of 2 week culture period of gingival fibroblasts. Bone sialoprotein mRNA was only expressed in rhBMP-2 50ng/ml group and rhBMP-2 100ng/ml group of 2-week culture period. These results suggest that rhBMP-2 stimulates osteoblastic differentiation in human periodontal ligament cells and gingival fibroblasts in vitro.

• Reproductive and Developmental Biology
• /
• v.31 no.4
• /
• pp.267-272
• /
• 2007

Human embryonic stem (ES) cells are derived from the inner cell mass of the preimplantation embryo and have the capacity to differentiate into various types of cells in the body. Hence, these cells may potentially be an indefinite source of cells for cell therapy in various degenerative diseases including neuronal disorders. For clinical applications of human ES cells, directed differentiation of these cells would be necessary. The objective of this study is to develop the culture condition for the expansion of neural precursor cells derived from human ES cells. Human ES cells were able to differentiate into neural precursor cells upon a stepwise culture condition. Neural precursor cells were propagated up to 5000-fold in cell numbers over 12-week period of culture and evaluated for their characteristics. Expressions of sox1 and pax6 transcripts were dramatically up-regulated along the differentiation stages by RT-PCR analysis. In contrast, expressions of oct4 and nanog transcripts were completely disappeared in neural precursor cells. Expressions of nestin, pax6 and sox1 were also confirmed in neural precursor cells by immunocytochemical analysis. Upon differentiation, the expanded neural precursor cells differentiated into neurons, astrocytes, and oligodendrocytes. In immunocytochemical analysis, expressions of type III ${\beta}$-tubulin and MAP2ab were observed Presence of astrocytes and oligodendrocytes were also confirmed by expressions of GFAP and O4, respectively. Results of this study demonstrate the feasibility of long-term expansion of human ES cell-derived neural precursor cells in vitro, which can be a potential source of the cells for the treatment of neurodegenerative disorders.

• The Journal of the Convergence on Culture Technology
• /
• v.8 no.3
• /
• pp.15-23
• /
• 2022

This study is a non-equivalent control group quasi-experimental design to test the effectiveness of educational programs on human tissue donation for nursing students to change the perception of human tissue donation. The subjects of the study were 75 nursing students located in U city, 38 in the experimental group and 37 in the control group. The data were analyzed using SPSS/WIN 23.0. The average age was 22.39±0.75 years old, women 84.2% and there was no difference in general characteristics and prior data between the two groups. Knowledge (F=-8.921, p<).001, Attitude (F=-5.414, p<).001, perception of death (F=-3.075, p=).004) showed a significant difference, and the educational intervention of nursing students developed to promote human tissue donation showed a positive effect on human tissue donation. When programs to be applied to educational institutions that train not only medical personnel but also experts in other fields must be developed, the establishment and stability of the human tissue donation culture will be solidified. It is also suggested that health care campaigns and education in public educational institutions should be conducted together.

• Tuberculosis and Respiratory Diseases
• /
• v.50 no.2
• /
• pp.205-212
• /
• 2001

Background : The information on nasal transport and the metabolism of peptides have been obtained from pharmacokinetic investigations in experimental animals. However, there are no transport and metabolic studies of human nasal epithelial cells. In this study, the permeability characteristics and the metabolic properties of in vitro human nasal cell monolayers were investigated. Material and Methods : Normal human inferior nasal conchal tissue samples were obtained from patients undergoing endoscopic nasal cavitary surgery. The specimens were cultured in a transwell using an air-liquid Interface (ALI) culture, and the transepithelial electrical resistance (TEER) value of the blank filter and confluent cell monolayers were measured. To determine the % leakage of mannitol, $4{\mu}mol%$ $^{14}C$-labelled mannitol was added and the % leakage was measured every 10 minute for 1 hour. Result : Human nasal epithelial cells in the primary culture grew to a confluent monolayer within 7 days and expressed microvilli. The tight junction between the cells was confirmed by transmission electron microscopy. The TEER value of the blank filter, fifth day and seventh day reached $108.5\;ohm.cm^2$, $141\;ohm.cm^2$ and $177.5\;ohm.cm^2$, respectively. Transcellular % leakage of the $^{14}$-mannitol at 10, 20, 30, 40, 50 and 60 minutes was $35.67{\pm}5.43$, $34.42{\pm}5.60$, $32.75{\pm}5.71$, $31.76{\pm}4.22$, $30.96{\pm}3.49$ and $29.60{\pm}3.68\;%$, respectively. Conclusion : The human nasal epithelial monolayer using ALI culture techniques is suitable for a transcellular permeability study. The data suggests that human nasal epithelial cells In an ALI culture technique shows some promise for a nasal transport and metabolism study.

• The International Journal of Costume Culture
• /
• v.8 no.2
• /
• pp.85-96
• /
• 2005

The purposes of this study were to examine young women's fashion and their values in South Korea in the 1970s and to explore the dynamics of how the changes in values affected fashion. Fifteen Korean women who were college students in the 1970s were interviewed. With industrialization and acculturation to Western customs in the 1970s, Korean women's fashion reflected the permeation of new ideas and behaviors into the culture. New ideas of 'pursuing a career' or 'gaining professional success' rooted in the women's movement were most important and were reflected in the prevalence of casual and comfortable styles. However, 'having a good husband and being a good wife' was also important. In addition to the transition in gender roles, Korea was fast moving from a collectivist to an individualistic society and underwent the process of melding traditional and newly-adopted values in their acculturation to the West. This study helps us better understand Korea's acculturation process through dress and the dynamics involved between fashion and value changes.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.9 no.6
• /
• pp.423-427
• /
• 2004

A simple purification procedure of bioactive human granulocyte macrophage colony stimulating factor (hGM-CSF) secreted in rice cell suspension culture has previously been described. In this study the protein was purified to apparent homogeneity with an overall yield of $80.1\%$ by ammonium sulfate precipitation and a single chromatographic step involving FPLCanion exchange chromatography. The purified hGM-CSF revealed at least five glycosylated forms ranging from $21.5{\~}29$ kDa, and its biological activity was independent of the glycosylation pattern. This is the first purification report of recombinant hGM-CSF to apparent homogeneity from rice cell suspension cultures.

• Proceeding of Spring/Autumn Annual Conference of KHA
• /
• 2009.11a
• /
• pp.55-58
• /
• 2009

Traditional pile-buildings in Southeast Asia has high utility. Their floors are lifted above the ground to stave off humidity in the house, increase the amenity and hygienic condition, fend off fierce animals or harmful animals like rat, and protect the house from being submerged in flood waters. Such traditional pile-buildings have Austronesian saddle-backed roof commonly, and are built through various techniques such as joining and mortising, without use of nails. The roof has a considerably large proportional appearance, and for this formal characteristics, the roof is symbolized as boat on occasions. The roof has dual structures or is lifted in some cases to facilitate the ventilation, and the wall is formed to maximize the ventilation. This housing types the hierarchy of foundation, housing space, and roof, which implies religious symbolism that human is above animal and God is above human. However, housing types in Southeast Asia have very different detailed form and culture, depending on the ethnic and religious characteristics. As explained above, this study examines the common aspects and diversity based on the form and culture of traditional housing of Southeast Asia and provides useful basic academic data.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.9 no.1
• /
• pp.12-16
• /
• 2004

Partitioning of human granulocyte-macrophage colony stimulating factor (hGM-CSF) was achieved in the aqueous two-phase systems (ATPSs) using a crude extract of transgenic tobacco cell suspension culture. This study examined the effects of polyethylene glycol (PEG) molecular weight and concentration and the effects of sodium phosphate concentration in different PEG/sodium phosphate systems on the partition coefficient, K. The best ATPS system was 5% PEG 8,000/1.6 M sodium phosphate after 2 h of incubation at room temperature. In this system, hGM-CSF was partitioned in the PEG-rich phase with a yield of 57.99% and K$\_$hGM-CSF/ of 8.12. In another system, 3% PEG 10,000/1.6 M sodium phosphate, hGM-CSF was also partitioned primarily in the top phase with a yield of 45.66% and K$\_$hGM-CSF/ of 7.64 after 2 h of incubation at room temperature.