• Journal of Dairy Science and Biotechnology
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• 제29권1호
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• pp.49-54
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• 2011

담즙산 분해효소(Bile salt hydrolase, EC 3.5.1.24) 활성은 담즙산의 카르복실기와 결합되어 있는 아미노기(glycine or taurine)와의 amide 결합을 끊는 작용을 하며, 이 효소 활성은 사람이나 동물의 장내 미생물들에서 널리 분포하고 있다. 노인 분변으로부터 분리한 여러 균주의 Enterococcus faecalis중에서 BSH activity가 가장 높은 CU30-2를 선발하였다. BSH 유전자를 pET22b expression vector에 클로닝하여 Escherichia coli BL21(DE3) Gold를 이용하여 단백질을 발현하였다. 6x His-tag이 있는 BSH 효소를 $Ni^+$-NTA agarose column을 이용하여 분리 정제하였고, 6가지의 다른 담즙산염을 이용하여 기질 특이성을 비교하였다. E. faecalis CU30-2의 BSH 효소는 glycine이 결합된 담즙산염에 대한 효소활성이 taurine이 결합된 것에 대한 활성보다 약 50배 정도 높게 나타났다. 이 효소의 최적 pH와 온도는 각각 7.0과 40$^{\circ}C$로 확인되었다.

• Journal of Pharmaceutical Investigation
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• 제33권2호
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• pp.105-112
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• 2003

Selective COX (cyclooxygenase)-2 inhibitors including celecoxib have been shown to induce apoptosis and cell cycle changes in various tumor cells. New inhibitors are recently being developed as chemomodulating agents. We evaluated celecoxib and screened 150 synthetic compounds for anti-proliferative activities in vitro. Effects of celecoxib on COX activity, cell growth, cell cycle distribution, and apoptosis induction were determined in A549 COX-2 overexpressing human non-small cell lung cancer (NSCLC) cells. The COX inhibition of celecoxib increased with concentration up to 82% at $1\;{\mu}M$ after 24 hr exposure. Forty ${\mu}M$ and $50\;{\mu}M$ of ce1ecoxib induced $G_1$ arrest, and TUNEL-positive apoptotic cells, respectively. Among 150 compounds, several compounds were selected for having greater COX-2 inhibitory activity and higher selectivity than celecoxib with growth inhibitory activity. Celecoxib showed concentration-dependent COX inhibitory activity, and ability to induce cell cycle arrest and apoptosis in human NSCLC cells in vitro. Among synthetic analogues screened, several compounds showed promising in vitro activity as COX-2 inhibitory anticancer agents, which warrant further evaluation in vitro and in vivo.

• 한국통신학회논문지
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• 제17권4호
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• pp.323-335
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• 1992

본 논문에서는 인간의 시각특성을 반영한 적응 DCT 영상 부호화에 관하여 연구하였다. 인간의 시각은 특정한 중간 주파수 대역에 가장 민감하며, 저주파 대역과 고주파 대역에 대하여 상대적으로 덜 민감하다. 이러한 특성은 많은 심리시각적 실험을 통하여 MTF(Modulation transfer function)라는 수식으로 모델링되었다. DCT는 공간영역의 에너지를 주파수 영역으로 변환하므로 MTF를 효율적으로 이용할 수 있다. 또한 시각의 공간 마스킹 특성에 의하여 변화가 많은 영역의 잡음은 변화가 적은 영역의 잡음에 비하여 인간의 시각에 덜 민감하다. 제안된 부호화 방법은 시각의 공간주파수 반응 특성을 양자화 행렬로 채택하였으며, 영상의 변화도에 따라 변환 블럭을 차등양자화 함으로써 주관적 화질의 향상을 꾀하였다. 각 블럭의 변화도를 측정하기 위한 방법으로 공간 영역에서 간단한 연산을 행하였으며, 이에 따라 변화가 적은 블럭을 상대적으로 정확하게 양자화하였다. 실험결과 낮은 비트율에서 시각특성을 반영한 제안된 부호화 방법이 주관적으로 우수한 성능을 보임을 확인하였다.

• 대한의생명과학회지
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• 제12권4호
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• pp.399-403
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• 2006

Cervical cancer is one of the most prevalent cancers developed in women worldwide, and human papillomavirus (HPV) type 16 is the most common agent linked to human cerivical carcinoma. Viral oncogenes E6 and E7 are selectively ratined and expressed in carcinoma cells infected with human papillomavirus type 16 and cooperated with each other in immortalization and transformation of primary keratinocytes. Because of HPV oncogenesis mechanism was not completely solved, the more studies be required thoroughly. In the present study, to investigate the telomere independent role of telomerase in HPV oncogenesis, we constructed the E6 mutant, E7, E6/E7 and hTERT over-expressed stable cells with a telomerase negative cell line, SW13. Expressions of Inserted genes were measured by RT-PCR. E6, E7 and hTERT genes were well expressed in each cell lines comparing with the control groups. By analyzing the cell morphology under the microscope, hTERT clone size was a more smaller than the mock control but oncogene expressed clones were slightly lengthened the marginal region. In addition, hTERT cells has also, a tendency of brief dividing time compared to the mock control. To determine whether telomerase activity associated with a HPV oncogenesis by oncoprotein expression, we performed the PCR based TRAP assay and Northern blot analysis. In TRAP assay data, telomerase activities in hTERT and oncogene clones were more increased than the mock control. In addition, SW13/ E6/E7 cells appeared a extremely increased activity than any other clones. Induced TERT mRNA by E6/E7 wasn't, however, detected in Northern blotting. In conclusion, these findings suggest that telomerase activity closely associated the HPV oncogenesis and E6/E7 co-expression is a most important factor of telomerase activity.

• Journal of Periodontal and Implant Science
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• 제23권2호
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• pp.228-242
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• 1993

Some therapeutic agents and medicaments may lead to pathologic changes in the gingival tissue, especially on the cultured human gingival fibroblasts. The purpose of this study was to investigate on the effect of diphenylhydantoin, retinoic acid to the human gingival fibroblast. Human gingival fibroblasts were cultured from the healthy gingiva of patients with orthodontic patients. Gingival fibroblasts were trypsinized and transferred to the wells of 96 well microtest plates. Next day, the medium was removed, fibroblasts were washed with HBSS, and the washed cells were cultured in growth medium added 5 or $10{\mu}g/ml$ of diphenylhydantoin, $10^{-5}M$, $10^{-6}M$ and $10^{-7}M$ of retinoic acid and glycyrrhetinic acid. The passage number of cultured fibroblasts were fifth and eighth. The cell morphology was examined by inverted microscope, the cell number was counted by hemocytometer, and cell activity was measured by the growth and proliferatiton assay using MTT assay. The fifth experiments were performed and statistical significance was measured by ANOVA. The cell morphology in the presence of retinoic acid was round irrespective of the presence of diphenylhydantoin and glycyrrhetinic acid(Fig 2-6). The proliferation of cells was not changed by diphenylhydantoin(Table 1). The cell activity showed the tendency to increase at the concentration of $10{\mu}$'/, of diphenylhydantoin (Table 2). The cell activity in the presence of retinoic acid glycyrrhetinic acid was decreased, and the increased cell activity by diphenylhydantoin was decreased by retinoic acid and glycyrrhetinic acid at the concentration of $10^{-7}M$(Table 3-5). These results suggested that the increased cell activity by diphenylhydantoin might be modulated by retinoic acid and glycyrrhetinic acid.

• 미생물학회지
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• 제38권4호
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• pp.255-255
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• 2002

For rapid and sensitive measurement of antiviral activities, application of a recombinant virus containing firefly luciferase gene was attempted. Recombinant human cytomegalovirus (HCMV) containing luciferase gene driven by HCMV late gene pp28 promoter (HCMV/pp28-luc) was used to test the antiviral activities of three known compounds and the result was compared with results from the conventional plaque assay for measuring the production of infectious viruses. When human fibroblast cells were infected with HCMV/pp28-luc, luciferase activity was observed at 2 days after infection and reached maximum at 6 days after infection, whereas the production of infectious virus was maximal at 4 days after infection. The antiviral activities of ganciclovir, acyclovir, and papaverine were measured in HFF cells infected with HCMV/PP28-luc and the luciferase activity was compared with the infectious virus titers. Luciferase activity decreased as the concentration of ganciclovir or papaverine increased, while there was a slight decrease in luciferase activity with acyclovir. The level of the decrease in Luciferase activity was comparable to the level of decrease in the production of infectious virus. Therefore, the antiviral assay using recombinant virus HCMV/pp28-luc resulted in sensitivity similar to the conventional plaque assay with a significant reduction in assay time.

• 대한임상검사과학회지
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• 제39권1호
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• pp.1-6
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• 2007

Cervical cancer is one of the most prevalent cancers developed in women worldwide, and human papillomavirus(HPV) type 16 is the most common agent linked to human cerivical carcinoma. Viral oncogenes E6 and E7 are selectively retained and expressed in carcinoma cells infected with human papillomavirus type 16 and cooperate with each other in the immortalization and transformation of primary keratinocytes. Because the HPV oncogenesis mechanism was not completely solved, more thorough studies are required. ln the present study, we investigated the telomere independent role of telomerase in HPV oncogenesis, we constructed the E6 mutant, E7, E6/E7 and hTERT over-expressed stable cells with a telomerase negative cell line, SW13. Expressions of inserted genes were measured by RT-PCR. E6, E7 and hTERT genes were well expressed in each cell lines when compared with the control groups. By analyzing the cell morphology under the microscope, hTERT clone size was a smaller than the mock control but oncogene expressed clones had a slightly lengthened marginal region. In addition, hTERT cells also has a tendency of brief dividing time compared to the mock control. To determine whether telomerase activity was associated with a HPV oncogenesis by oncoprotein expression, we performed the PCR based TRAP assay and a Northern blot analysis. In TRAP assay data, telomerase activities in hTERT and oncogene clones increased compared to the mock control. In addition, SW13/E6/E7 cells showed an extremely increased activity compared to the other clones. Induced hTERT mRNA by E6/E7 wasn't, however, detected in Northern blotting. In conclusion, these findings suggest that telomerase activity is closely associated with the HPV oncogenesis and E6/E7 co-expression is a most important factor of telomerase activity.

• 정보과학회 논문지
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• 제43권7호
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• pp.781-785
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• 2016

실제 환경에서 사람의 일상적인 활동을 학습하는 기술은 스마트 비서나 자율지능 로봇과 같은 인지 지능 시스템 개발을 위해 필요한 핵심 기술이다. 일상을 예측하는 대다수의 연구들은 센서 데이터의 패턴과 일상 활동 사이의 직접적인 상관관계를 탐색하는 것에 집중하였다. 하지만 일상에서의 인간 활동은 하나의 레이블로 표현하기 어려운 다수의 사건 집합이고 또한 서술 가능한 특성을 지니고 있다. 본고에서는 일상을 구성하는 사건 요소들을 우선 인식하고, 이후 일상 활동을 학습 및 예측하는 방법을 제안한다. 제안하는 방법은 개인의 일상에서 웨어러블 장치와 스마트폰으로부터 수집된 일인칭 시점의 멀티 센서 데이터로부터 위치 좌표, 장면 영상, 그리고 신체적 움직임에 기인한 사건 요소들을 각각 인식한 뒤, 이 정보들이 특정 활동 내역에 따라 조합되는 규칙을 학습하여 최종적으로 사용자의 일상 활동을 예측한다. 두 명의 실험 참가자가 각각 2주간 수집한 센서 데이터를 이용하여 실험한 결과는 제안한 방법이 센서 데이터로부터 추출된 특징을 일차적으로 사용하여 분류하는 기존의 방법과 비교하여 향상된 성능을 보였다.

• 한국인터넷방송통신학회논문지
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• 제22권5호
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• pp.177-183
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• 2022

최근 COVID-19로 인해 홈 트레이닝의 관심도가 증가하고 있다. 이에 따라 HAR(human activity recognition) 기술을 홈 트레이닝에 적용한 연구가 진행되고 있다. 기존 HAR 분야의 논문에서는 동적인 자세보다는 앉기, 일어서기와 같은 정적인 자세들을 분석한다. 본 논문은 동적인 운동 자세를 분석하여 사용자의 운동 자세 정확도를 보여주는 딥러닝 모델을 제안한다. AI hub의 피트니스 이미지를 blaze pose를 사용하여 사람의 자세 데이터를 분석한다. 3개의 딥러닝 모델: RNN(recurrnet neural networks), LSTM(long short-term memory networks), CNN(convolution neural networks)에 대하여 실험을 진행한다. RNN, LSTM, CNN 모델의 f1-score는 각각 0.49, 0.87, 0.98로 CNN 모델이 가장 적합하다는 것을 확인하였다. 이후 연구로는, 다양한 학습 데이터를 사용하여 더 많은 운동 자세를 분석할 예정이다.

• 한국식품조리과학회지
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• 제23권1호통권97호
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• pp.90-98
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• 2007

The purpose of this study was to apply the activity-based costing (ABC) model to restaurant menu costing. The overhead cast of six entr${\acute{e}}$es in XYZ restaurant was calculated for all levels of activity. When comparing activity-based costing with traditional costing applied to BBQ pork rib and an assorted seafood platter, the total difference of costs between two items was 2,191 won in activity-based costing and 600 won in traditional costing. The average food cast percentage of the six entr${\acute{e}}$es was 27% using traditional costing, while the average activity-based cost percentage was 40%. Therefore, there was a 13% difference between the actual margin volume and the expected margin volume. The application of activity-based costing to the restaurant industry would be a milestone from a cost point of view as well as from a process point of view. In particular, the limitation that traditional costing only accounts for food costs could be overcome b considering the overhead cost as an important part of the cast structure. Furthermore, activity-based costing would not only help to reduce the costs associated with the process of analyzing the activities but it would also provide more accurate cost information for menu pricing.