• 대한인간공학회지
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• 제19권1호
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• pp.49-61
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• 2000

An experiment was designed to evaluate Fitts' law for the three-dimensional virtual pointing task and to compare the three input devices; Spaceball, Spacemouse, and 3D-Mouse. The result showed that Fitts law fitted poorly for the three-dimensional pointing tasks with relatively low coefficients of determinant. Three reasons, high degree-of-freedom, dynamic egocentric viewpoint change, and clutching problem were discussed to explain the poor fitness of Fitts' law. In terms of device comparison, the 3D-Mouse was superior to the other input devices. Also, the stereoscopic display significantly increased the performance. The results of this study can be used for the design of virtual control tasks and the selection of suitable input devices.

• IMMUNE NETWORK
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• 제14권1호
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• pp.1-6
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• 2014

Epstein-Barr virus (EBV) is etiologically associated with a variety of diseases including lymphoproliferative diseases, lymphomas, carcinomas, and autoimmune diseases. Humans are the only natural host of EBV and limited species of new-world monkeys can be infected with the virus in experimental conditions. Small animal models of EBV infection, required for evaluation of novel therapies and vaccines for EBV-associated diseases, have not been available. Recently the development of severely immunodeficient mouse strains enabled production of humanized mice in which human immune system components are reconstituted and express their normal functions. Humanized mice can serve as infection models for human-specific viruses such as EBV that target cells of the immune system. This review summarizes recent studies by the author's group addressing reproduction of EBV infection and pathogenesis in humanized mice.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2004년도 춘계학술발표대회
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• pp.195-195
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• 2004

To discover germ cell-specific transcripts, we prepared a cDNA library from adult testes of 35-day old mice and subtracted it with mRNA from the testes of juvenile mice. Real-time RT-PCR analysis indicated that 42 cDNA clones in the subtracted library were expressed more intensely in the adult testes than in the juvenile testes. One clone identified by subtraction is expressed preferentially in the late spermatid and is located on chromosome 17E3 in mouse and 2p22 in human. (omitted)

• Journal of Microbiology and Biotechnology
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• 제12권3호
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• pp.526-529
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• 2002

Lysis of erythrocytes isolated from human, rabbit, and mouse blood samples was investigated with the culture supernatant of Xenorhabdus nematophilus in a primary form. Prior to use, the culture supernatant of the bacteria was concentrated and the concentrate was dialyzed against Tris-HCl buffer (10 mM, pH 8.1) by ultrafiltration using PM-5 membrane with a molecular weight cut-off of 5,000. At $30^{\circ}C$, the supernatant exhibited no lytic activity towards three types of erythrocytes. However, at $4^{\circ}C$, the supernatant showed selective lytic activity towards rabbit erythrocytes within 90 min. yet did not lyze human or mouse erythrocytes. Microscopic examination clearly revealed that most of the rabbit erythrocytes had been fumed into ghost forms.

• Clinical and Experimental Reproductive Medicine
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• 제21권2호
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• pp.207-214
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• 1994

This study was carried out to develop the methodology of chromosome preparation from blood cultures in mammals which included human, mouse, cattle and pig. For karyotyping, 0.5-5.0ml of peripheral blood were collected and cultured. The satisfactory results were obtained from macroculture and microculture in all species. In culture, the patterns of cell growth were no difference among media except serum concentration and mitogen supplement. The presence of mitogen and fetal bovine serum in medium significantly affected the mitotic index. The optimal culture condition was 37$^{\circ}C$ for 3 days. And the concentration of colcemid and reincubation time also affected the chromosome morphology. In harvest, chromosome patterns were mainly affected on hypotonic treatment which included treated time and temperature, dropwise of fixative solution, and drying after slide preparation.

• Biomolecules & Therapeutics
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• 제4권4호
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• pp.334-338
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• 1996

Antigenicity of DA-3285, human recombinant erythropoietin which was produced from mammalian cells, was examined in guinea pigs and mice. In active systemic anaphylactic test, mild anaphylactic signs were observed in guinea pigs sensitized subcutaneously with DA-3285 or DA-3285 incorporated with complete Freund's adjuvant(CFA) when challenged with high dose(1000 IU/Kg) of DA-3285. Other groups showed negative responses. In mouse-rat passive cutaneous anaphylaxis test, 20% sera of mice immunized with DA-3285 or DA-3285 mixed with aluminum hydroxide(alum) showed mild positive responses. In the case of indirect haemagglutination reaction(IHA) test, when sheep red blood cells coated with DA-3285 was incubated with mouse serum, all the serum samples were showed negative responses. These results suggest that DA-3285 has a very weak antigenic potential and probably would not induce systemic allergic reactions in clinical uses.

• Archives of Pharmacal Research
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• 제23권4호
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• pp.288-301
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• 2000

A series of 2-alkyl, 2-aryl, and 2-piperazinyl benzimidazole-4,7-dione derivatives (7a-h) and 16m-o) were prepared, and their cytotoxicities were tested against three cancer cell lines (mouse lymphocytic leukemia cell line P388, and human gastric carcinoma cell lines SNU-1 and SNU-16). These compounds showed potent cytotoxicity against all of three cell lines tested, and especially SNU-16 was sensitive to them. 2-Aryl (7g,h) and 2-piperazinyl benzimidazole-4,7-dione derivative (I6 m) were more potent than mitomycin C against P388 and SNU-16. Among benzimidazole-4,7-dione derivatives with alkyl group at position 2, 7a had the most potent cytotoxicity against all of the cell lines tested.

• Applied Microscopy
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• 제50권
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• pp.11.1-11.3
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• 2020

The human turbinate-derived mesenchymal stem cells (hTMSCs), which were DiI-labeled and transplanted into the subretinal space in degenerating mouse retina, were observed in retinal vertical sections processed for rhodopsin (a marker for rod photoreceptor) by confocal microscope with differential interference contrast (DIC) filters. The images clearly demonstrated that DiI-labeled hTMSCs have rhodopsin-immunoreactive appendages, indicating differentiation of transplanted hTMSC into rod photoreceptor. Conclusively, the finding suggests therapeutic potential of hTMSCs in retinal degeneration.

• Clinical and Experimental Pediatrics
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• 제46권6호
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• pp.536-540
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• 2003

목 적 : 인유를 탐식한 폐포 대식세포는 면역세포화학 반응에 관여하며, 기존의 염색법에서보다 면역세포화학기법에 의해 잘 관찰될 수 있음을 규명하여, 인유 흡인을 확인할 수 있는 진단에 활용하고자 본 연구를 시행하였다. 방 법 : 총 64마리의 백서를 대상으로 소량(0.05 mL)의 모유를 코를 통하여 기도에 흡인시킨 후 지정 시간별로 기관을 도관한 다음, 기관지폐포 세척을 실시하여 얻은 세포에 대하여 면역세포화학적 염색과 Oil Red O 염색을 실시하고 폐포 대식세포들의 탐식 정도를 관찰하였다. 결 과 : 인유 흡인 2시간 후에 Lactalbumin에 대한 양성 대식세포가 관찰되었으며 8시간에 최고이었고 24시간에 감소하였으며 48시간에도 양성 대식세포가 관찰되었다. 그러나 Oil Red O 염색에서 폐포 대식세포들의 검출률은 미미하였다. 결 론 : 본 실험의 결과는 소량의 인유 흡인을 면역세포화학적 기법에 의하여 기관지폐포 세척 액에서 검출할 수 있다는 것을 시사하는 것이었다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2003년도 추계학술대회 논문요약집
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• pp.11-11
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• 2003